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The map label for this gene is gatD

Identifier: 209395932

GI number: 209395932

Start: 2842171

End: 2843211

Strand: Reverse

Name: gatD

Synonym: ECH74115_3070

Alternate gene names: 209395932

Gene position: 2843211-2842171 (Counterclockwise)

Preceding gene: 209397449

Following gene: 209396656

Centisome position: 51.03

GC content: 48.51

Gene sequence:

>1041_bases
ATGAAATCAGTGGTGAATGATACTGATGGTATCGTGCGCGTTGCAGAAAGCGTCATTCCTGAAATTAAACATCAGGATGA
GGTGCGGGTAAAAATTGCCAGCTCGGGCTTATGTGGTTCCGATTTACCCAGGATATTTAAAAATGGTGCACATTATTATC
CAATAACGTTAGGCCATGAATTTAGCGGCTATATTGATGCGGTAGGATCCGGTGTTGATGATTTACACCCTGGCGATGCG
GTTGCCTGTGTGCCGTTATTACCCTGTTTTACTTGTCCAGAGTGTTTGAAAGGGTTTTATTCCCAGTGCGCAAAATATGA
TTTTATTGGCTCACGGCGTGATGGTGGATTTGCCGAATATATTGTCGTTAAGCGAAAAAATGTCTTTGCTCTACCCACGG
ATATGCCTATTGAGGATGGGGCTTTTATTGAGCCGATTACCGTTGGCCTGCATGCTTTTCATTTAGCGCAAGGTTGTGAG
AATAAAAACGTTATTATTATTGGTGCCGGAACCATTGGCCTGCTGGCTATTCAGTGCGCTGTCGCGCTGGGAGCAAAGAG
TGTGACGGCTATCGACATTAGCTCAGAAAAACTGGCACTGGCAAAATCTTTCGGTGCGATGCAAACATTTAACAGTAGCG
AAATGAGCGCGCCGCAAATGCAGAGCGTTTTACGCGAACTGCGCTTTAATCAGCTTATCCTCGAGACGGCTGGCGTACCG
CAAACTGTCGAACTGGCGGTAGAGATTGCCGGTCCTCATGCCCAACTGGCACTGGTGGGCACGTTGCATCAGGATCTGCA
TTTAACATCGGCAACGTTTGGCAAAATATTGCGTAAAGAGCTGACGGTTATCGGCAGTTGGATGAACTACTCCAGCCCTT
GGCCGGGGCAGGAGTGGGAAACGGCGAGCCGGTTGCTGACAGAACGTAAGTTAAGCCTGGAGCCATTAATCGCTCACCGT
GGAAGCTTTGAAAGCTTCGCCCAGGCGGTGCGTGACATCGCTCGTAATGCTATGCCGGGCAAAGTGTTGCTCATTCCCTG
A

Upstream 100 bases:

>100_bases
GGCGTAGAGCGCGTGGCTTTATTAAACAAGAGAAAGTCGTTCTCGCAGAATAATTTTTACCTGAGGGGGAGGTTATCCCC
CTTAACAATTAGGAGTTTTT

Downstream 100 bases:

>100_bases
AACCGCGGGCCAGCGTGATGCTGGCCCGGTATTGTGCAAAACAGATCATTCACCAATGGTCCCCCTTCGTTTACACTAGC
CACAATTGAATATGGGTAAA

Product: galactitol-1-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDA
VACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHR
GSFESFAQAVRDIARNAMPGKVLLIP

Sequences:

>Translated_346_residues
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDA
VACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHR
GSFESFAQAVRDIARNAMPGKVLLIP
>Mature_346_residues
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDA
VACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHR
GSFESFAQAVRDIARNAMPGKVLLIP

Specific function: Converts galactitol 1-phosphate to tagatose 6-phosphate

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family

Homologues:

Organism=Homo sapiens, GI156627571, Length=351, Percent_Identity=27.3504273504274, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI4501929, Length=224, Percent_Identity=29.4642857142857, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI4501939, Length=338, Percent_Identity=24.8520710059172, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI156523966, Length=338, Percent_Identity=24.8520710059172, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4501933, Length=226, Percent_Identity=29.646017699115, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI34577061, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI71565154, Length=338, Percent_Identity=24.8520710059172, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI262073058, Length=234, Percent_Identity=26.0683760683761, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI71743840, Length=234, Percent_Identity=26.0683760683761, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788407, Length=346, Percent_Identity=100, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI1788073, Length=335, Percent_Identity=29.5522388059701, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787863, Length=333, Percent_Identity=31.5315315315315, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI226510992, Length=318, Percent_Identity=29.874213836478, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790045, Length=344, Percent_Identity=26.7441860465116, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790718, Length=336, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082125, Length=337, Percent_Identity=28.486646884273, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788075, Length=312, Percent_Identity=25.3205128205128, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786825, Length=317, Percent_Identity=25.8675078864353, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786518, Length=272, Percent_Identity=28.6764705882353, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081918, Length=227, Percent_Identity=30.8370044052863, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=325, Percent_Identity=29.5384615384615, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17562878, Length=229, Percent_Identity=32.3144104803493, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25146526, Length=341, Percent_Identity=24.9266862170088, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71997431, Length=350, Percent_Identity=24, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17565904, Length=323, Percent_Identity=25.077399380805, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988145, Length=214, Percent_Identity=29.9065420560748, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=354, Percent_Identity=29.0960451977401, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322619, Length=338, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6319955, Length=338, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6319258, Length=236, Percent_Identity=29.6610169491525, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6319257, Length=236, Percent_Identity=28.8135593220339, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=353, Percent_Identity=23.5127478753541, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319621, Length=290, Percent_Identity=27.2413793103448, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17137530, Length=204, Percent_Identity=34.3137254901961, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737897, Length=204, Percent_Identity=34.8039215686275, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45551930, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI221457811, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550770, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17737895, Length=232, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_ECO57 (P0A9S4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F90990
- PIR:   H85835
- RefSeq:   NP_288593.1
- RefSeq:   NP_310921.1
- ProteinModelPortal:   P0A9S4
- SMR:   P0A9S4
- EnsemblBacteria:   EBESCT00000027324
- EnsemblBacteria:   EBESCT00000055811
- GeneID:   916596
- GeneID:   956811
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3254
- KEGG:   ecs:ECs2894
- GeneTree:   EBGT00050000008795
- HOGENOM:   HBG753318
- OMA:   VCVEERP
- ProtClustDB:   PRK10309
- BioCyc:   ECOL83334:ECS2894-MONOMER
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11695

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N; SSF50129 GroES_like

EC number: =1.1.1.251

Molecular weight: Translated: 37391; Mature: 37391

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHE
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCC
FSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEY
CHHHHHHHCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCA
EEEECCCEEECCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
VALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
HHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWE
CEEEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TASRLLTERKLSLEPLIAHRGSFESFAQAVRDIARNAMPGKVLLIP
HHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITLGHE
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCC
FSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEY
CHHHHHHHCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEE
IVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCA
EEEECCCEEECCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
VALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVP
HHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
QTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWE
CEEEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TASRLLTERKLSLEPLIAHRGSFESFAQAVRDIARNAMPGKVLLIP
HHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796