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The map label for this gene is aroP

Identifier: 209397094

GI number: 209397094

Start: 124648

End: 126018

Strand: Reverse

Name: aroP

Synonym: ECH74115_0118

Alternate gene names: 209397094

Gene position: 126018-124648 (Counterclockwise)

Preceding gene: 209399999

Following gene: 209398277

Centisome position: 2.26

GC content: 52.52

Gene sequence:

>1371_bases
ATGGAAGGTCAACAGCACGGCGAGCAGCTAAAGCGCGGCCTTAAAAACCGCCATATTCAGCTTATCGCGCTGGGTGGCGC
GATAGGGACAGGGTTATTCCTGGGTAGCGCCTCCGTAATACAGTCCGCAGGGCCAGGGATTATCCTGGGTTACGCCATTG
CTGGTTTTATCGCCTTTCTGATCATGCGTCAGCTGGGTGAAATGGTGGTCGAAGAACCTGTCGCAGGCTCCTTTAGCCAC
TTTGCTTATAAATACTGGGGCAGCTTTGCTGGCTTCGCTTCTGGCTGGAACTACTGGGTACTGTACGTTTTAGTTGCCAT
GGCAGAGCTGACTGCTGTGGGTAAATACATTCAGTTCTGGTATCCGGAAATCCCAACCTGGGTTTCTGCCGCCGTGTTCT
TTGTGGTGATTAACGCCATCAACCTGACCAACGTAACAGTGTTTGGTGAGATGGAGTTCTGGTTTGCCATTATCAAAGTT
ATTGCGGTAGTAGCGATGATCATCTTCGGCGGCTGGCTGCTGTTCAGTGGTAACGGCGGTCCGCAGGCAAGCGTTAGCAA
CCTGTGGGATCAGGGCGGTTTCCTGCCGCACGGCTTCACCGGGCTGGTGATGATGATGGCGATTATCATGTTCTCGTTCG
GTGGTCTGGAACTGGTGGGGATCACCGCAGCAGAAGCTGATAACCCGGAGCAAAGTATCCCGAAAGCAACTAACCAGGTT
ATCTACCGCATCCTGATTTTCTATATTGGTTCGTTAGCCGTTCTGCTCTCACTGATGCCGTGGACCCGCGTTACCGCCGA
TACCAGTCCGTTTGTGCTGATCTTCCACGAGTTAGGCGATACCTTTGTGGCGAATGCGCTGAACATCGTGGTACTGACTG
CGGCGCTCTCCGTGTACAACAGCTGCGTATATTGCAACAGCCGTATGCTGTTTGGTCTGGCACAACAGGGTAACGCGCCA
AAAGCGCTGGCGTCTGTCGATAAACGCGGCGTACCGGTTAACACCATTCTGGTGTCTGCGCTGGTTACAGCATTGTGCGT
ATTGATTAACTATCTTGCTCCGGAATCCGCATTTGGCCTGTTAATGGCACTGGTGGTATCCGCACTGGTGATCAACTGGG
CGATGATCAGTCTGGCGCATATGAAGTTCCGTCGCGCCAAGCAGGAACAAGGCGTGGTAACTCGCTTCCCTGCTCTGCTT
TATCCGCTGGGTAACTGGATCTGCCTGCTGTTTATGGCGGTGGTACTGGTGATTATGCTGATGACCCCAGGAATGGCGAT
TTCGGTATACCTGATCCCGGTATGGCTGGTGGTGTTAGGTATCGGCTATCTGTTTAAAGAGAAAACCGCAAAAGCCGTAA
AAGCACATTAA

Upstream 100 bases:

>100_bases
CTTCTTTGATGTAAACAAATTAATACAACAAACGGAATTGCAAACTTACACACGCATCACTGCGTAGATCAAAAAAACAA
TCACCGCACGAGGTTTCATG

Downstream 100 bases:

>100_bases
TCTCTCTACGCCCTCACCCGTACAGGGTGAGGGCAATAATCTTTACACCAACGCCCCGTAAATCGTCAGTAGCGCAATCA
CCACCACGACCACGAACGAG

Product: aromatic amino acid transporter

Products: L-tryptophan [Cytoplasm]; Proton [Cytoplasm]; Proton [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: General aromatic amino acid permease

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSH
FAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIKV
IAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAP
KALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALL
YPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKTAKAVKAH

Sequences:

>Translated_456_residues
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSH
FAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIKV
IAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAP
KALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALL
YPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKTAKAVKAH
>Mature_456_residues
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSH
FAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIKV
IAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAP
KALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALL
YPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKTAKAVKAH

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of the aromatic amino acids (phenylalanine, tyrosine, and tryptophan)

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family

Homologues:

Organism=Homo sapiens, GI258645169, Length=430, Percent_Identity=23.2558139534884, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI258614005, Length=431, Percent_Identity=22.737819025522, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI110347453, Length=356, Percent_Identity=23.876404494382, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI258614003, Length=431, Percent_Identity=22.737819025522, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI181337167, Length=292, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4507047, Length=388, Percent_Identity=21.6494845360825, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1786302, Length=456, Percent_Identity=99.1228070175439, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=430, Percent_Identity=65.1162790697674, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1790653, Length=461, Percent_Identity=40.1301518438178, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1786602, Length=453, Percent_Identity=43.4878587196468, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI48994972, Length=431, Percent_Identity=40.1392111368909, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI87081915, Length=456, Percent_Identity=36.4035087719298, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI87081708, Length=465, Percent_Identity=35.2688172043011, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1789017, Length=432, Percent_Identity=35.8796296296296, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1788480, Length=392, Percent_Identity=35.969387755102, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI87081869, Length=173, Percent_Identity=26.0115606936416, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17531343, Length=380, Percent_Identity=25.5263157894737, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17533459, Length=376, Percent_Identity=22.6063829787234, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320772, Length=449, Percent_Identity=35.6347438752784, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324061, Length=440, Percent_Identity=33.6363636363636, Blast_Score=235, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6324924, Length=456, Percent_Identity=33.1140350877193, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324553, Length=398, Percent_Identity=33.9195979899498, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6320717, Length=406, Percent_Identity=32.2660098522167, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6322892, Length=431, Percent_Identity=34.338747099768, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321053, Length=423, Percent_Identity=31.2056737588652, Blast_Score=203, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319824, Length=402, Percent_Identity=31.592039800995, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324990, Length=449, Percent_Identity=30.5122494432071, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324059, Length=440, Percent_Identity=32.9545454545455, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321629, Length=400, Percent_Identity=30.25, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319543, Length=398, Percent_Identity=30.9045226130653, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6320251, Length=405, Percent_Identity=29.3827160493827, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6319542, Length=406, Percent_Identity=28.5714285714286, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319608, Length=418, Percent_Identity=28.7081339712919, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322967, Length=399, Percent_Identity=29.8245614035088, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324981, Length=417, Percent_Identity=28.537170263789, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320364, Length=472, Percent_Identity=26.6949152542373, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24667468, Length=406, Percent_Identity=24.1379310344828, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI221512776, Length=370, Percent_Identity=22.1621621621622, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666159, Length=370, Percent_Identity=22.1621621621622, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI161077963, Length=418, Percent_Identity=23.2057416267943, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROP_ECO57 (Q8X968)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85494
- PIR:   D90643
- RefSeq:   NP_285808.1
- RefSeq:   NP_308143.1
- ProteinModelPortal:   Q8X968
- EnsemblBacteria:   EBESCT00000026597
- EnsemblBacteria:   EBESCT00000057940
- GeneID:   913640
- GeneID:   956805
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0122
- KEGG:   ecs:ECs0116
- GeneTree:   EBGT00050000009004
- HOGENOM:   HBG492579
- OMA:   YKYWGNF
- ProtClustDB:   PRK10238
- BioCyc:   ECOL83334:ECS0116-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 49533; Mature: 49533

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x23b57dac)-; HASH(0x238ec428)-; HASH(0x23b4b4bc)-; HASH(0x2387ac40)-; HASH(0x246b0984)-; HASH(0x23a3c580)-; HASH(0x23921318)-; HASH(0x23af40b8)-; HASH(0x238763e0)-; HASH(0x23b56404)-; HASH(0x2393eb20)-; HASH(0x23a4a068)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFL
CCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHH
IMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFW
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
YPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNGG
CCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
PQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHCCHHHHHH
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHHH
SCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGL
HHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAVVLVIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTPGMAISVYLIPVWLVVLGIGYLFKEKTAKAVKAH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAFL
CCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHH
IMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQFW
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
YPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNGG
CCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
PQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQV
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHCCHHHHHH
IYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVYN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHHHH
SCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFGL
HHHHCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAVVLVIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTPGMAISVYLIPVWLVVLGIGYLFKEKTAKAVKAH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-tryptophan [Periplasm]; Proton [Periplasm]; Proton [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] Proton [Periplasm] + L-tyrosine [Periplasm] = Proton [Cytoplasm] + L-tyrosine [Cytoplasm] Proton [Periplasm] + L-phenylalanine [Periplasm] = Proton [Cytoplasm] +

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796