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The map label for this gene is truC

Identifier: 209397015

GI number: 209397015

Start: 3749799

End: 3750581

Strand: Reverse

Name: truC

Synonym: ECH74115_4054

Alternate gene names: 209397015

Gene position: 3750581-3749799 (Counterclockwise)

Preceding gene: 209395886

Following gene: 209397840

Centisome position: 67.31

GC content: 53.77

Gene sequence:

>783_bases
ATGCTGGAAATACTCTATCAGGATGAATGGCTGGTTGCGGTAAATAAACCCTCCGGCTGGCTGGTTCACCGTAGCTGGCT
GGATCGCGACGAGAAAGTAGTGGTCATGCAAACCGTGCGTGACCAGATAGGCCAGCATGTTTTTACTGCTCATCGTCTGG
ACCGACCCACTTCTGGTGTGTTGTTGATGGGATTATCCAGCGAGGCCGGACGGCTGCTGGCACAACAGTTTGAACAGCAC
CAAATCCAGAAACGTTACCATGCGATTGTGCGCGGCTGGTTGATGGAAGAAGCGGTGCTGGATTATCCACTGGTGGAAGA
ACTGGACAAAATCGCTGATAAATTTGCCCGCGAAGATAAAGGCCCGCAGCCAGCAGTGACGCATTATCGCGGTCTGGCGA
CCGTAGAAATGCCGGTAGCGACCGGACGTTACCCGACCACGCGCTACGGCCTGGTGGAACTGGAGCCGAAAACCGGACGC
AAACATCAGCTTCGCCGACATCTTGCCCATTTGCGTCATCCGATTATTGGCGATAGCAAACATGGCGATTTACGCCAGAA
TCGCAGCGGTGCTGAGCATTTTGGTCTTCAGCGGTTAATGCTGCATGCCAGTCAGTTGTCATTGACGCATCCTTTTACTG
GTGAGCCGCTGACTATTCACGCGGGCCTGGACGACACCTGGATGCAGGCGTTATCACAATTTGGCTGGCGCGGTCTGCTT
CCTGAAAATGAAAGGGTTGAGTTCAGTGCGCCATCGGGTCAGGATGGAGAGAGAAGTTCCTAA

Upstream 100 bases:

>100_bases
CCTATTATGAAATGGCGCTGGCGACGGACCATCCGCAACGCGCGCTTATTCTGGCTGAGTTGGAAAAACTGGATGCTCTT
TTTGCGGATGATGCGAGCTA

Downstream 100 bases:

>100_bases
TCCAGGGAGAAAACGGTATGGCGGAAATTGGTATTTTTGTCGGCACCATGTACGGAAATTCGCTGTTAGTGGCGGAAGAA
GCAGAAGCAATTCTGACCGC

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQH
QIQKRYHAIVRGWLMEEAVLDYPLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLTIHAGLDDTWMQALSQFGWRGLL
PENERVEFSAPSGQDGERSS

Sequences:

>Translated_260_residues
MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQH
QIQKRYHAIVRGWLMEEAVLDYPLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLTIHAGLDDTWMQALSQFGWRGLL
PENERVEFSAPSGQDGERSS
>Mature_260_residues
MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQH
QIQKRYHAIVRGWLMEEAVLDYPLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLTIHAGLDDTWMQALSQFGWRGLL
PENERVEFSAPSGQDGERSS

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family

Homologues:

Organism=Homo sapiens, GI221316667, Length=186, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221316663, Length=204, Percent_Identity=30.8823529411765, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI23308689, Length=178, Percent_Identity=29.2134831460674, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1789155, Length=260, Percent_Identity=99.6153846153846, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=33.4763948497854, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1786244, Length=224, Percent_Identity=34.8214285714286, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788946, Length=237, Percent_Identity=28.2700421940928, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320168, Length=180, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324506, Length=187, Percent_Identity=26.7379679144385, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583449, Length=186, Percent_Identity=31.7204301075269, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583447, Length=186, Percent_Identity=31.7204301075269, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583445, Length=186, Percent_Identity=31.7204301075269, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921090, Length=179, Percent_Identity=31.2849162011173, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUC_ECO57 (Q8X6T6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91085
- PIR:   E85930
- RefSeq:   NP_289346.1
- RefSeq:   NP_311678.1
- ProteinModelPortal:   Q8X6T6
- SMR:   Q8X6T6
- EnsemblBacteria:   EBESCT00000024905
- EnsemblBacteria:   EBESCT00000055867
- GeneID:   916545
- GeneID:   957078
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4107
- KEGG:   ecs:ECs3651
- GeneTree:   EBGT00050000009216
- HOGENOM:   HBG750656
- OMA:   GCDRLML
- ProtClustDB:   PRK11112
- BioCyc:   ECOL83334:ECS3651-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 29709; Mature: 29709

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01129 PSI_RLU

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGV
CCEEEEECCEEEEEECCCCEEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDYPLVEELDKIADKFAREDK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
GPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGRKHQLRRHLAHLRHPIIGDSK
CCCCHHHHHCCCEEEEECCCCCCCCCCEECEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLTIHAGLDDTWMQALSQFGWRGLL
CCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCC
PENERVEFSAPSGQDGERSS
CCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGV
CCEEEEECCEEEEEECCCCEEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDYPLVEELDKIADKFAREDK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
GPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGRKHQLRRHLAHLRHPIIGDSK
CCCCHHHHHCCCEEEEECCCCCCCCCCEECEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLTIHAGLDDTWMQALSQFGWRGLL
CCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCC
PENERVEFSAPSGQDGERSS
CCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796