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The map label for this gene is thiK [H]

Identifier: 209395825

GI number: 209395825

Start: 1465958

End: 1466782

Strand: Direct

Name: thiK [H]

Synonym: ECH74115_1486

Alternate gene names: 209395825

Gene position: 1465958-1466782 (Clockwise)

Preceding gene: 209400489

Following gene: 209396392

Centisome position: 26.31

GC content: 52.85

Gene sequence:

>825_bases
GTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTTTCATCCCGTCACGAC
GTTTAATAGTGGGCTTAGTGGCGGTAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATC
CTGATGCGCCGCAGTCCGCGTTCTTGCACCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCAT
TTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGAGGTAAAAACGTATTTGCCGGATACCAACGAACTGGC
AGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGC
AGCAAAGCGATCCGGCGCGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAAGCGCGGGAACCACGGCCT
TTACGCTTAAGTCCATTGCATATGGATGTCCACGCCGGAAATTTAGTGCATAGCGCGTCAGGGTTAAAACTCATCGACTG
GGAGTATGCAGGAGATGGTGATATCGCGCTGGAACTGGCGGCGGTGTGGGTGGAAAATACTGAACAGCACCGGCAATTGG
TCAATGACTATGCCACTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTG
ATGCTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATGACACCTG
GCGGCAGCTATTAATAAAACAATAA

Upstream 100 bases:

>100_bases
TACGTGCTGTACTCCAGCGCCTCTGGCAACGTTAACGCTCCGACCCTACAAATGCAGCTGATGCTGGTGCAGACGGGCGA
AATTATCTGGTCAGGTAAAG

Downstream 100 bases:

>100_bases
GGAGAGCAGTGTGGGTCCAGTAATGTTGGATGTCGAAGGTTACGAACTGGACGCGGAAGAGCGTGAAATACTGGCGCATC
CGCTGGTGGGAGGGCTGATT

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLHQYRALSQLPACIAPKPH
LYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ

Sequences:

>Translated_274_residues
MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLHQYRALSQLPACIAPKPH
LYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
>Mature_273_residues
PFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSAFLHQYRALSQLPACIAPKPHL
YLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPL
RLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLLM
LKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=274, Percent_Identity=98.9051094890511, Blast_Score=554, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 32421; Mature: 32290

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSA
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHH
FLHQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFG
HHHHHHHHHHCCHHCCCCCCEEEECEEEEEECCCCCEECCCCCHHHHHHHHHHHCCCCCC
WRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCC
GLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
CCEEEEEEECCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
HHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHH
FLHQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFG
HHHHHHHHHHCCHHCCCCCCEEEECEEEEEECCCCCEECCCCCHHHHHHHHHHHCCCCCC
WRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCC
GLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
CCEEEEEEECCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
HHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA