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The map label for this gene is FrvX3 [H]

Identifier: 20806952

GI number: 20806952

Start: 453878

End: 454861

Strand: Direct

Name: FrvX3 [H]

Synonym: TTE0443

Alternate gene names: 20806952

Gene position: 453878-454861 (Clockwise)

Preceding gene: 20806951

Following gene: 20806956

Centisome position: 16.88

GC content: 40.75

Gene sequence:

>984_bases
ATGAGTGTGAATGTGGAACTTATAAAAAAGCTAACACAGGCTTTTGGACCTTCGGGAAGTGAGGAGAAGGTTTTTGAGAT
TATAAGAGAGGAAGTCAAAGGTTTTTGCGATGAAATTACTCATGATGCGATGGGAAACATGATATGTGTGAAAAAGGGAA
AAGGGAAAAAGATAATGGTGGCTGCTCATGCTGATGAAATAGGCATTATGGTTACGCATATTGAAGAGGAAGGCTTCTTG
AGGTTCACAACGATAGGTGGCGTTTATGTAGAGCACCTTGTAGGAAGAAGAGTTAAGTTTAAAAATGGTACAGTAGGAGT
AATTGGAGTAGAGCATTTGGAGGATAAAAAGGATTTTAAACTGGAGAAGCTTTACATAGATATAGGTGCAAAAGATAAAA
AAGAGGCAGAAGAGCTTGTCAAAATAGGGGAGAGTGGAAGCTTCGTAGGAGAGTTTGTAGAAGCTGGAGATAGGTTAATT
TCCAAGGCTTTTGACGACAGGATAGGATGCTATGTGGCGATTGAAGCGCTAAAAAACGTGAAGACAGAAAATGAGCTGTA
TTTTGTTTTCACAGTGCAAGAAGAGGTAGGCTTAAGAGGTGCTACTACAGCCGCTTACAGTATTAATCCTGATTTTGCTA
TTGCTGTGGATGTAACTGCAACGGGAGATACTCCTAAAGCGAAGAAAATGGCGGTAGCTCTCGGTAAAGGTGCAGCAATA
AAGGTAATGGACAGATCGATAATTGTGAGCCCTTCTGTGAGAGATATGATGATTGAAGTGGCGAAAGAGAACAGTATTCC
GTATCAATTAGAAATACTGGAATTTGGCGGTACTGATGCAGGAGCGATTCACCTTTCAAGAGGGGGAGTACCCTCTGGAG
TGATTTCTATTCCCACAAGGTATGTTCACAGCGTTTCAGAGATGGTGGATAAGAAGGACGTAGAGGCAAGTATTAACCTC
CTGATAAAAATACTGGAAAAATAA

Upstream 100 bases:

>100_bases
GATACATCCATTCTTTCAACTCTGTAGCTAAGCTGAGTGACTTTGAAAATACAGTTAAGCTTGTGGACTTAGTGATTAAA
AATATTGAGGAGGTATTGAA

Downstream 100 bases:

>100_bases
AGCAAAAGACCCTGCTTTTTAAGCGGGGTCTTTTTATTTATAGGCTTATGCACTTTCACTGGAGGTGCTATTTTTTGAAT
TTACAAGCCAGTTGACTATA

Product: cellulase M-like protein

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSVNVELIKKLTQAFGPSGSEEKVFEIIREEVKGFCDEITHDAMGNMICVKKGKGKKIMVAAHADEIGIMVTHIEEEGFL
RFTTIGGVYVEHLVGRRVKFKNGTVGVIGVEHLEDKKDFKLEKLYIDIGAKDKKEAEELVKIGESGSFVGEFVEAGDRLI
SKAFDDRIGCYVAIEALKNVKTENELYFVFTVQEEVGLRGATTAAYSINPDFAIAVDVTATGDTPKAKKMAVALGKGAAI
KVMDRSIIVSPSVRDMMIEVAKENSIPYQLEILEFGGTDAGAIHLSRGGVPSGVISIPTRYVHSVSEMVDKKDVEASINL
LIKILEK

Sequences:

>Translated_327_residues
MSVNVELIKKLTQAFGPSGSEEKVFEIIREEVKGFCDEITHDAMGNMICVKKGKGKKIMVAAHADEIGIMVTHIEEEGFL
RFTTIGGVYVEHLVGRRVKFKNGTVGVIGVEHLEDKKDFKLEKLYIDIGAKDKKEAEELVKIGESGSFVGEFVEAGDRLI
SKAFDDRIGCYVAIEALKNVKTENELYFVFTVQEEVGLRGATTAAYSINPDFAIAVDVTATGDTPKAKKMAVALGKGAAI
KVMDRSIIVSPSVRDMMIEVAKENSIPYQLEILEFGGTDAGAIHLSRGGVPSGVISIPTRYVHSVSEMVDKKDVEASINL
LIKILEK
>Mature_326_residues
SVNVELIKKLTQAFGPSGSEEKVFEIIREEVKGFCDEITHDAMGNMICVKKGKGKKIMVAAHADEIGIMVTHIEEEGFLR
FTTIGGVYVEHLVGRRVKFKNGTVGVIGVEHLEDKKDFKLEKLYIDIGAKDKKEAEELVKIGESGSFVGEFVEAGDRLIS
KAFDDRIGCYVAIEALKNVKTENELYFVFTVQEEVGLRGATTAAYSINPDFAIAVDVTATGDTPKAKKMAVALGKGAAIK
VMDRSIIVSPSVRDMMIEVAKENSIPYQLEILEFGGTDAGAIHLSRGGVPSGVISIPTRYVHSVSEMVDKKDVEASINLL
IKILEK

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=334, Percent_Identity=36.2275449101796, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI87082417, Length=350, Percent_Identity=31.1428571428571, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788727, Length=334, Percent_Identity=29.940119760479, Blast_Score=157, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 35673; Mature: 35542

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNVELIKKLTQAFGPSGSEEKVFEIIREEVKGFCDEITHDAMGNMICVKKGKGKKIMV
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEE
AAHADEIGIMVTHIEEEGFLRFTTIGGVYVEHLVGRRVKFKNGTVGVIGVEHLEDKKDFK
EECCCCEEEEEEEECCCCCEEEEEECHHHHHHHHCCEEEECCCCEEEEEHHHCCCCCCCE
LEKLYIDIGAKDKKEAEELVKIGESGSFVGEFVEAGDRLISKAFDDRIGCYVAIEALKNV
EEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHCC
KTENELYFVFTVQEEVGLRGATTAAYSINPDFAIAVDVTATGDTPKAKKMAVALGKGAAI
CCCCEEEEEEEEEHHCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHEEEEECCCCEE
KVMDRSIIVSPSVRDMMIEVAKENSIPYQLEILEFGGTDAGAIHLSRGGVPSGVISIPTR
EEECCEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCEECCHH
YVHSVSEMVDKKDVEASINLLIKILEK
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVNVELIKKLTQAFGPSGSEEKVFEIIREEVKGFCDEITHDAMGNMICVKKGKGKKIMV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEE
AAHADEIGIMVTHIEEEGFLRFTTIGGVYVEHLVGRRVKFKNGTVGVIGVEHLEDKKDFK
EECCCCEEEEEEEECCCCCEEEEEECHHHHHHHHCCEEEECCCCEEEEEHHHCCCCCCCE
LEKLYIDIGAKDKKEAEELVKIGESGSFVGEFVEAGDRLISKAFDDRIGCYVAIEALKNV
EEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEHHHHCC
KTENELYFVFTVQEEVGLRGATTAAYSINPDFAIAVDVTATGDTPKAKKMAVALGKGAAI
CCCCEEEEEEEEEHHCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHEEEEECCCCEE
KVMDRSIIVSPSVRDMMIEVAKENSIPYQLEILEFGGTDAGAIHLSRGGVPSGVISIPTR
EEECCEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCEECCHH
YVHSVSEMVDKKDVEASINLLIKILEK
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA