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The map label for this gene is OppA4 [H]

Identifier: 20806867

GI number: 20806867

Start: 374750

End: 376501

Strand: Direct

Name: OppA4 [H]

Synonym: TTE0350

Alternate gene names: 20806867

Gene position: 374750-376501 (Clockwise)

Preceding gene: 20806866

Following gene: 20806868

Centisome position: 13.93

GC content: 39.5

Gene sequence:

>1752_bases
GTGCGAAGGCTCTTAAGTGTATTATTGGTTTTAATGTTGGTGACGGGTGCACTGGCAGGCTGTGGACAAAAGAGCCAGGA
AGCAGCTGATAAAACCCAGCAAAGTGAAACAGCCCAGCAACCTGCCCAAGAAACTACCTATCAGAGAAATGAAACTCTTT
ATGCTGGAGGCGGATTGTGGGCACCACCGAGCAACTGGAATCCGCTTACACCATGGAATGCAGTAACAGGTACAGTAGGA
TTGATTTACGAAACTTTGTTTAATTATGACCCGCTTAAAAACGAATTCATTCCATGGTTAGCCGAAAAAGGTGAATGGAC
CTCTGATAATACTTATCAAGTAACATTAAGAGATGGACTTACTTGGTCAGATGGTCAGCCTTTGACTTCAGAGGATGTAA
AATTCACTTTTGAAATAGCAAAAGAACACCCTGAAATCTATTATAGCCCAATGTGGCAATGGTTGCAATCTGTAGATACT
CCTGATAATAAGACTGTGGTATTTAAATTTTCAACTTCCCATTATCACGAATGGCAATATAACTTATATCAAATTCCTAT
TATACCAAAACATATTTGGCAGAATAAATCTAAAGATGATTTGCTGAATGGTGCTAATGAAAAGGCAATTGGTTCAGGAC
CTTACTTATTTGATACTTACAGCAATGACCGCATGGTATATAAGAGAAATGATAACTGGTGGGGTATAAAAGCTCTGGGA
CTTACTCCACAACCTAAGAGAATAGTGTACTTAATTGTTCCAAGTAATAACGTAGCTCTCGGAATGTTGATGAAAGGAGA
ACTGGATGTAAGTAATTTCTTCCTCCCTGGTATACCGACGTTAAAAGCTAATTATGGAATAACGACTTTCTATGACAATC
CACCATACATGATTCCTGATAATACAGTGTTTATGTTTATCAATGTGACTAAAGAACCTCTTAGCAATGTAGAACTAAGA
CGCGCCATGGCTTATGCTATAAATCCAAAAGTAATAGCTGAAAAGGTGTACGAAAATCAGGTTGAACCTTCAAATCCTCT
AGGTTTTGTGCCTGCTAAGTCTTGGGAAGAATATTATGATAAGAACGTAGTTTCACAGTATGGATTTAGCTATGATCCTA
ATAAAGCAAAAGCGATTTTGGATGCTGCAGGATTTAAATTGGGTAACGATGGAGTGAGGACAACTCCAGATGGCAAGAAG
TTTAAATTAGAAATTAGCGTTCCGTATGGTTGGACTGACTGGATGGAAGCAGCTAAGATTGTTGCAGACCAGTTAAAGGC
TGTAGGAATTGATGCAGAAGCAAAATTCCCTGATTATAGCAAATATTGGGAAGATTTAACTAAAGGGACATTTGATTTAT
CTTTCAACAACTTCGGAAGCCAAGTAACCTCAACACCATGGACGCTTTACAACTGGCTCTTCAATCCTATAACAGGAGAT
GCTCAATACAATGGTAACTTTGGGCGCTTTGATGTAGAAGGGTTGCAGGATATGGTGGCAGCTTTAAATGAAGCAAAACC
TGGAACAGATGAAGCTAAAAAGGCTGCTTCAAAATTGGAAGAAGTATTCTTAAAGAACATACCGGCCATACCGCTTTGGT
ACAATGGTCTCTGGTTCCAGGCATCAAATGCAGCATGGGAAAATTGGCCAACAGAACATGACCCATATGCTTACCCAGTA
ACTTGGGGAGGCAGATGGCAGACAGGCGGTACAATGATGATGATGAAAATAAAACCGAAAGCTTCGCAATAA

Upstream 100 bases:

>100_bases
GCCTTTTTAATCTAAAAATTGAGAAAGGGGTGATAAAAAGAAAAGGTATATTTAAAAAATTTATAGACTTTGAAAACTAT
TTCAAAAGGGGGATTGTGAG

Downstream 100 bases:

>100_bases
TAAATGGGGGTATATAGATGTTACGGTATTTGATTAAGAAATTGTACATCTACATTTTGACTTTCTTTTTTGCAGTGACA
ATCGACTGGCTGATCCCCAG

Product: ABC-type dipeptide/oligopeptide/nickel transport systems, periplasmic components

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MRRLLSVLLVLMLVTGALAGCGQKSQEAADKTQQSETAQQPAQETTYQRNETLYAGGGLWAPPSNWNPLTPWNAVTGTVG
LIYETLFNYDPLKNEFIPWLAEKGEWTSDNTYQVTLRDGLTWSDGQPLTSEDVKFTFEIAKEHPEIYYSPMWQWLQSVDT
PDNKTVVFKFSTSHYHEWQYNLYQIPIIPKHIWQNKSKDDLLNGANEKAIGSGPYLFDTYSNDRMVYKRNDNWWGIKALG
LTPQPKRIVYLIVPSNNVALGMLMKGELDVSNFFLPGIPTLKANYGITTFYDNPPYMIPDNTVFMFINVTKEPLSNVELR
RAMAYAINPKVIAEKVYENQVEPSNPLGFVPAKSWEEYYDKNVVSQYGFSYDPNKAKAILDAAGFKLGNDGVRTTPDGKK
FKLEISVPYGWTDWMEAAKIVADQLKAVGIDAEAKFPDYSKYWEDLTKGTFDLSFNNFGSQVTSTPWTLYNWLFNPITGD
AQYNGNFGRFDVEGLQDMVAALNEAKPGTDEAKKAASKLEEVFLKNIPAIPLWYNGLWFQASNAAWENWPTEHDPYAYPV
TWGGRWQTGGTMMMMKIKPKASQ

Sequences:

>Translated_583_residues
MRRLLSVLLVLMLVTGALAGCGQKSQEAADKTQQSETAQQPAQETTYQRNETLYAGGGLWAPPSNWNPLTPWNAVTGTVG
LIYETLFNYDPLKNEFIPWLAEKGEWTSDNTYQVTLRDGLTWSDGQPLTSEDVKFTFEIAKEHPEIYYSPMWQWLQSVDT
PDNKTVVFKFSTSHYHEWQYNLYQIPIIPKHIWQNKSKDDLLNGANEKAIGSGPYLFDTYSNDRMVYKRNDNWWGIKALG
LTPQPKRIVYLIVPSNNVALGMLMKGELDVSNFFLPGIPTLKANYGITTFYDNPPYMIPDNTVFMFINVTKEPLSNVELR
RAMAYAINPKVIAEKVYENQVEPSNPLGFVPAKSWEEYYDKNVVSQYGFSYDPNKAKAILDAAGFKLGNDGVRTTPDGKK
FKLEISVPYGWTDWMEAAKIVADQLKAVGIDAEAKFPDYSKYWEDLTKGTFDLSFNNFGSQVTSTPWTLYNWLFNPITGD
AQYNGNFGRFDVEGLQDMVAALNEAKPGTDEAKKAASKLEEVFLKNIPAIPLWYNGLWFQASNAAWENWPTEHDPYAYPV
TWGGRWQTGGTMMMMKIKPKASQ
>Mature_583_residues
MRRLLSVLLVLMLVTGALAGCGQKSQEAADKTQQSETAQQPAQETTYQRNETLYAGGGLWAPPSNWNPLTPWNAVTGTVG
LIYETLFNYDPLKNEFIPWLAEKGEWTSDNTYQVTLRDGLTWSDGQPLTSEDVKFTFEIAKEHPEIYYSPMWQWLQSVDT
PDNKTVVFKFSTSHYHEWQYNLYQIPIIPKHIWQNKSKDDLLNGANEKAIGSGPYLFDTYSNDRMVYKRNDNWWGIKALG
LTPQPKRIVYLIVPSNNVALGMLMKGELDVSNFFLPGIPTLKANYGITTFYDNPPYMIPDNTVFMFINVTKEPLSNVELR
RAMAYAINPKVIAEKVYENQVEPSNPLGFVPAKSWEEYYDKNVVSQYGFSYDPNKAKAILDAAGFKLGNDGVRTTPDGKK
FKLEISVPYGWTDWMEAAKIVADQLKAVGIDAEAKFPDYSKYWEDLTKGTFDLSFNNFGSQVTSTPWTLYNWLFNPITGD
AQYNGNFGRFDVEGLQDMVAALNEAKPGTDEAKKAASKLEEVFLKNIPAIPLWYNGLWFQASNAAWENWPTEHDPYAYPV
TWGGRWQTGGTMMMMKIKPKASQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=355, Percent_Identity=28.7323943661972, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789887, Length=421, Percent_Identity=25.1781472684086, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787551, Length=416, Percent_Identity=22.1153846153846, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66218; Mature: 66218

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLSVLLVLMLVTGALAGCGQKSQEAADKTQQSETAQQPAQETTYQRNETLYAGGGLW
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCC
APPSNWNPLTPWNAVTGTVGLIYETLFNYDPLKNEFIPWLAEKGEWTSDNTYQVTLRDGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCCCCCCEEEEEEECCC
TWSDGQPLTSEDVKFTFEIAKEHPEIYYSPMWQWLQSVDTPDNKTVVFKFSTSHYHEWQY
EECCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCEECEEE
NLYQIPIIPKHIWQNKSKDDLLNGANEKAIGSGPYLFDTYSNDRMVYKRNDNWWGIKALG
EEEEECCCHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEC
LTPQPKRIVYLIVPSNNVALGMLMKGELDVSNFFLPGIPTLKANYGITTFYDNPPYMIPD
CCCCCCEEEEEEECCCCEEEEEEEECCCCCCCEECCCCCCEECCCCEEEEECCCCEECCC
NTVFMFINVTKEPLSNVELRRAMAYAINPKVIAEKVYENQVEPSNPLGFVPAKSWEEYYD
CEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHH
KNVVSQYGFSYDPNKAKAILDAAGFKLGNDGVRTTPDGKKFKLEISVPYGWTDWMEAAKI
HHHHHHCCCCCCCCHHHHHHHHCCCEECCCCCEECCCCCEEEEEEECCCCCHHHHHHHHH
VADQLKAVGIDAEAKFPDYSKYWEDLTKGTFDLSFNNFGSQVTSTPWTLYNWLFNPITGD
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCC
AQYNGNFGRFDVEGLQDMVAALNEAKPGTDEAKKAASKLEEVFLKNIPAIPLWYNGLWFQ
CEECCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEEE
ASNAAWENWPTEHDPYAYPVTWGGRWQTGGTMMMMKIKPKASQ
ECCCCCCCCCCCCCCEEEEEEECCEEECCCEEEEEEECCCCCC
>Mature Secondary Structure
MRRLLSVLLVLMLVTGALAGCGQKSQEAADKTQQSETAQQPAQETTYQRNETLYAGGGLW
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCC
APPSNWNPLTPWNAVTGTVGLIYETLFNYDPLKNEFIPWLAEKGEWTSDNTYQVTLRDGL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCCCCCCEEEEEEECCC
TWSDGQPLTSEDVKFTFEIAKEHPEIYYSPMWQWLQSVDTPDNKTVVFKFSTSHYHEWQY
EECCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCEEEEEEECCCEECEEE
NLYQIPIIPKHIWQNKSKDDLLNGANEKAIGSGPYLFDTYSNDRMVYKRNDNWWGIKALG
EEEEECCCHHHHHCCCCHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEC
LTPQPKRIVYLIVPSNNVALGMLMKGELDVSNFFLPGIPTLKANYGITTFYDNPPYMIPD
CCCCCCEEEEEEECCCCEEEEEEEECCCCCCCEECCCCCCEECCCCEEEEECCCCEECCC
NTVFMFINVTKEPLSNVELRRAMAYAINPKVIAEKVYENQVEPSNPLGFVPAKSWEEYYD
CEEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHH
KNVVSQYGFSYDPNKAKAILDAAGFKLGNDGVRTTPDGKKFKLEISVPYGWTDWMEAAKI
HHHHHHCCCCCCCCHHHHHHHHCCCEECCCCCEECCCCCEEEEEEECCCCCHHHHHHHHH
VADQLKAVGIDAEAKFPDYSKYWEDLTKGTFDLSFNNFGSQVTSTPWTLYNWLFNPITGD
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCC
AQYNGNFGRFDVEGLQDMVAALNEAKPGTDEAKKAASKLEEVFLKNIPAIPLWYNGLWFQ
CEECCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEEE
ASNAAWENWPTEHDPYAYPVTWGGRWQTGGTMMMMKIKPKASQ
ECCCCCCCCCCCCCCEEEEEEECCEEECCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]