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The map label for this gene is CelF [H]

Identifier: 20806854

GI number: 20806854

Start: 356265

End: 357575

Strand: Direct

Name: CelF [H]

Synonym: TTE0337

Alternate gene names: 20806854

Gene position: 356265-357575 (Clockwise)

Preceding gene: 20806853

Following gene: 20806855

Centisome position: 13.25

GC content: 34.55

Gene sequence:

>1311_bases
ATGTCTAAAAAAGATTTAAAAATTGCAGTTATAGGGGGTGGTTCTAGTTATACCCCTGAGCTTATTGAAGGGTTTATCAA
AAGATATGAAGAATTACCCGTAAGAGATATTTATTTAATGGACATAGAAGAAGGCAAAGAAAAACTTGAGATTGTTGGCG
GTCTTGCAAAAAGAATGGTAGAAAAAGCTGGGGTTGGTATAAACATTCATCTGACTTTAGACAGACGTGAAGCCATAAAA
GATGCAGATTTTGTTGTTACTCAATTCAGAGTAGGAGGAATAGATGCGAGAATTAGAGATGAAAAAATTCCTTTAAAGTA
TGATGTTATAGGACAAGAAACAACTGGACCCGGTGGTTTTGCAAAAGCACAGAGAACAATACCAGTTATACTAGATGTAT
GCAAAGACATAGAAGAACTTGCACCAAATGCGTGGCTTATCAATTTTACAAATCCTGCAGGTGTAATAACAGAAACGATT
TTAAAGCATACAAACGTAAAAGCGATCGGATTATGTAATGTACCTATAGGTATGGTACACGGTGTTGCAAAAATGCTTGA
GGTTGATTCAAAAAGAGTGTATATAGATTTTGCAGGGCTTAATCATTTAGTATGGGGTACACACATTTACTTAGATGGAG
AAGATATAACAGAAAAATTAATAGATAGTTTTGCAGGTGGTAAGTCTTTATCAATGAAAAACATACCAGAACTTCCTTGG
GATCCCGAATTTATCAAATCTCTTGGAATGTACCCTTGTCCTTATCATAGGTATTACTATTTAACTGATCAAATGTTAAA
AGAGCAAAAGAAAGAAGCTGCTACAGTAGGAACAAGAGGGGAAGTCGTTAAGAAATTAGAGAAAGAACTATTCGAACTAT
ATAAAGATCCGAATTTAAACGTAAAACCTCCTCAATTAGAAAAAAGAGGAGGAGCACACTATTCTGATGCTGCTTGTTCT
TTGATAAGTTCAATATATAATGATAAGAAAGATATACATGTGGTTAATGTGAGAAACAATGGGACGATTGCAGATTTGCC
GGATGATGTGGTAATAGAAACAAATGCAGTAGTTGATAAAAATGGTGCTCATCCTATAACTATTGGGCATGTATCACCAA
AAATAAGGGGTTTGATGCAAGTTGTTAAAGCATATGAAGAACTTACAATTGAAGCAGGGGTAAGAGGGGATTATTATGCG
GCTTTACAAGCGTTAACAATACATCCTTTAGTGCCATCTGCCACTATTGCTAAAAAGATACTTGATGATATAATTGAACA
GAATAAAGAATATTTACCGCAATATAAGTAA

Upstream 100 bases:

>100_bases
GCTGAGGATCATCTCATGAATGCTATCCTTGCGAAAGAGTTGATAACTGAAATGATTGAGCTCTATAAAATTATTTATCA
AACAAAGGGGGCTACTTATA

Downstream 100 bases:

>100_bases
TAATAGAAATTACCTCTCTTTGTTTTCTAGAATTATGTTTCAAAATCACTTTAACATGAGGAGTGAAATTCCTATGTCTA
AAGAATACAGATTTCCAGAA

Product: alpha-galactosidase/6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSKKDLKIAVIGGGSSYTPELIEGFIKRYEELPVRDIYLMDIEEGKEKLEIVGGLAKRMVEKAGVGINIHLTLDRREAIK
DADFVVTQFRVGGIDARIRDEKIPLKYDVIGQETTGPGGFAKAQRTIPVILDVCKDIEELAPNAWLINFTNPAGVITETI
LKHTNVKAIGLCNVPIGMVHGVAKMLEVDSKRVYIDFAGLNHLVWGTHIYLDGEDITEKLIDSFAGGKSLSMKNIPELPW
DPEFIKSLGMYPCPYHRYYYLTDQMLKEQKKEAATVGTRGEVVKKLEKELFELYKDPNLNVKPPQLEKRGGAHYSDAACS
LISSIYNDKKDIHVVNVRNNGTIADLPDDVVIETNAVVDKNGAHPITIGHVSPKIRGLMQVVKAYEELTIEAGVRGDYYA
ALQALTIHPLVPSATIAKKILDDIIEQNKEYLPQYK

Sequences:

>Translated_436_residues
MSKKDLKIAVIGGGSSYTPELIEGFIKRYEELPVRDIYLMDIEEGKEKLEIVGGLAKRMVEKAGVGINIHLTLDRREAIK
DADFVVTQFRVGGIDARIRDEKIPLKYDVIGQETTGPGGFAKAQRTIPVILDVCKDIEELAPNAWLINFTNPAGVITETI
LKHTNVKAIGLCNVPIGMVHGVAKMLEVDSKRVYIDFAGLNHLVWGTHIYLDGEDITEKLIDSFAGGKSLSMKNIPELPW
DPEFIKSLGMYPCPYHRYYYLTDQMLKEQKKEAATVGTRGEVVKKLEKELFELYKDPNLNVKPPQLEKRGGAHYSDAACS
LISSIYNDKKDIHVVNVRNNGTIADLPDDVVIETNAVVDKNGAHPITIGHVSPKIRGLMQVVKAYEELTIEAGVRGDYYA
ALQALTIHPLVPSATIAKKILDDIIEQNKEYLPQYK
>Mature_435_residues
SKKDLKIAVIGGGSSYTPELIEGFIKRYEELPVRDIYLMDIEEGKEKLEIVGGLAKRMVEKAGVGINIHLTLDRREAIKD
ADFVVTQFRVGGIDARIRDEKIPLKYDVIGQETTGPGGFAKAQRTIPVILDVCKDIEELAPNAWLINFTNPAGVITETIL
KHTNVKAIGLCNVPIGMVHGVAKMLEVDSKRVYIDFAGLNHLVWGTHIYLDGEDITEKLIDSFAGGKSLSMKNIPELPWD
PEFIKSLGMYPCPYHRYYYLTDQMLKEQKKEAATVGTRGEVVKKLEKELFELYKDPNLNVKPPQLEKRGGAHYSDAACSL
ISSIYNDKKDIHVVNVRNNGTIADLPDDVVIETNAVVDKNGAHPITIGHVSPKIRGLMQVVKAYEELTIEAGVRGDYYAA
LQALTIHPLVPSATIAKKILDDIIEQNKEYLPQYK

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=441, Percent_Identity=48.0725623582766, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1790560, Length=463, Percent_Identity=24.8380129589633, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 48552; Mature: 48421

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKDLKIAVIGGGSSYTPELIEGFIKRYEELPVRDIYLMDIEEGKEKLEIVGGLAKRMV
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHHH
EKAGVGINIHLTLDRREAIKDADFVVTQFRVGGIDARIRDEKIPLKYDVIGQETTGPGGF
HHHCCCEEEEEEECHHHHHCCHHEEEEEEEECCCCCEEECCCCCEEEEECCCCCCCCCCH
AKAQRTIPVILDVCKDIEELAPNAWLINFTNPAGVITETILKHTNVKAIGLCNVPIGMVH
HHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHH
GVAKMLEVDSKRVYIDFAGLNHLVWGTHIYLDGEDITEKLIDSFAGGKSLSMKNIPELPW
HHHHHHHCCCCEEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCC
DPEFIKSLGMYPCPYHRYYYLTDQMLKEQKKEAATVGTRGEVVKKLEKELFELYKDPNLN
CHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VKPPQLEKRGGAHYSDAACSLISSIYNDKKDIHVVNVRNNGTIADLPDDVVIETNAVVDK
CCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEECCCEEEC
NGAHPITIGHVSPKIRGLMQVVKAYEELTIEAGVRGDYYAALQALTIHPLVPSATIAKKI
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEECCCCCCHHHHHHH
LDDIIEQNKEYLPQYK
HHHHHHCCHHHCCCCC
>Mature Secondary Structure 
SKKDLKIAVIGGGSSYTPELIEGFIKRYEELPVRDIYLMDIEEGKEKLEIVGGLAKRMV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHHH
EKAGVGINIHLTLDRREAIKDADFVVTQFRVGGIDARIRDEKIPLKYDVIGQETTGPGGF
HHHCCCEEEEEEECHHHHHCCHHEEEEEEEECCCCCEEECCCCCEEEEECCCCCCCCCCH
AKAQRTIPVILDVCKDIEELAPNAWLINFTNPAGVITETILKHTNVKAIGLCNVPIGMVH
HHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHH
GVAKMLEVDSKRVYIDFAGLNHLVWGTHIYLDGEDITEKLIDSFAGGKSLSMKNIPELPW
HHHHHHHCCCCEEEEEECCCCEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCC
DPEFIKSLGMYPCPYHRYYYLTDQMLKEQKKEAATVGTRGEVVKKLEKELFELYKDPNLN
CHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
VKPPQLEKRGGAHYSDAACSLISSIYNDKKDIHVVNVRNNGTIADLPDDVVIETNAVVDK
CCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEECCCEEEC
NGAHPITIGHVSPKIRGLMQVVKAYEELTIEAGVRGDYYAALQALTIHPLVPSATIAKKI
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEECCCCCCHHHHHHH
LDDIIEQNKEYLPQYK
HHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]