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The map label for this gene is LeuB_2 [H]

Identifier: 20094651

GI number: 20094651

Start: 1204838

End: 1205845

Strand: Direct

Name: LeuB_2 [H]

Synonym: MK1215

Alternate gene names: 20094651

Gene position: 1204838-1205845 (Clockwise)

Preceding gene: 20094650

Following gene: 20094652

Centisome position: 71.08

GC content: 67.26

Gene sequence:

>1008_bases
GTGAACGCGTCGCCGACCGTCGTGGTAATCCCCGGGGACGGGATCGGGCCGGAAGTCATCGACGCGGCCCTGAAGGTCGT
GCGGGCGGTTCTGGGTGACGAACTCGAGATCGTGGAGGAACAGGCCGGGTACTCGCTGTGGAAGAAGAGGGGAGTCACGA
TCGAGGACGAGACGATCGAGCGCTGCCGCGAGGCGGACGCCATGCTCTTCGGCGCGTGCACGACTCCGGAGGACCCGGAG
GCCAAGAGTCCCATCGTGACGCTGCGGAAAGAGCTCGGACTCTACGCCAACGTCCGTCCCGCTCGCTCCTGGCCCGTACC
GCGACCTGTCGACACCGAGTTCGACCTGGTGATCGTCCGGGAGAACACCGAGGGTCTCTACACCGGATGCGAGTGCGAGA
TCCACGACGGTGTTACCGTCGCCCTGCGTAAGATCTCCGAGGAGGGTACGCGCAGGGTCGCTGAGGTCGCCTGCGATCTC
GCCGAGGAGCGCTCCGGTCGCGTGACGATAGTGCACAAGGCCAACGTGCTGAAGCTCACGTGCGGAACGTTCAAGCGCGT
CGCGGCGGAAACCGTGGAGCGTCGCGGTCTGGAGTGGGACGACGAGTACGTGGACGCGGCGGCGTACAAGCTGGTGCGTG
AGCCGGACAGCTTCGACGTGATCCTCACCTCCAACCTGTTCGGGGACATACTCTCGGACCTGGCGGCGGGTCTGATGGGG
AGCCTCGGGCTCGCACCCAGCGCGAACCTGGGGGACGACGCCGCCCTATTCGAGCCTGTACACGGGTCGGCACCCGACAT
CGCGGGTAAGGGGATCGCGAACCCGGTGGCGGCGATCCTCTCGGCCGCCATGATGCTGGATCACCTCGGGTACGGGGAGG
AGGCTCGGATCATCGAGCGTGCCGTCGAGGAGGTGCTGCGCGAGGGCGTGAGGACTCCCGACCTCGGCGGTTCCGCGACC
ACGGAGGAGGTGGCCGAGGCGATCGCGGAGCGTGTGGCTACCGGGTGA

Upstream 100 bases:

>100_bases
GACGGTCGGATACCGCCTAAGTTCCCACGATAACGGCCGATGACGAGCCTGCCCCGGCGGGATCGGTGACGAGGATTGGC
CTTTCCGAGGCCCGACGGGG

Downstream 100 bases:

>100_bases
GCGGGGGGCGTAGGGCGACCGGCTCGTACCTGGGATCACCCCCGAGCTCCACGAGGGCCACCTCGGGTAGCTCCTTAACC
CTGCGTGCGAACTCCTCGCT

Product: isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-isopropylmalate dehydrogenase; 3-IPM-DH; IMDH; IPMDH; Beta-IPM dehydrogenase; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNASPTVVVIPGDGIGPEVIDAALKVVRAVLGDELEIVEEQAGYSLWKKRGVTIEDETIERCREADAMLFGACTTPEDPE
AKSPIVTLRKELGLYANVRPARSWPVPRPVDTEFDLVIVRENTEGLYTGCECEIHDGVTVALRKISEEGTRRVAEVACDL
AEERSGRVTIVHKANVLKLTCGTFKRVAAETVERRGLEWDDEYVDAAAYKLVREPDSFDVILTSNLFGDILSDLAAGLMG
SLGLAPSANLGDDAALFEPVHGSAPDIAGKGIANPVAAILSAAMMLDHLGYGEEARIIERAVEEVLREGVRTPDLGGSAT
TEEVAEAIAERVATG

Sequences:

>Translated_335_residues
MNASPTVVVIPGDGIGPEVIDAALKVVRAVLGDELEIVEEQAGYSLWKKRGVTIEDETIERCREADAMLFGACTTPEDPE
AKSPIVTLRKELGLYANVRPARSWPVPRPVDTEFDLVIVRENTEGLYTGCECEIHDGVTVALRKISEEGTRRVAEVACDL
AEERSGRVTIVHKANVLKLTCGTFKRVAAETVERRGLEWDDEYVDAAAYKLVREPDSFDVILTSNLFGDILSDLAAGLMG
SLGLAPSANLGDDAALFEPVHGSAPDIAGKGIANPVAAILSAAMMLDHLGYGEEARIIERAVEEVLREGVRTPDLGGSAT
TEEVAEAIAERVATG
>Mature_335_residues
MNASPTVVVIPGDGIGPEVIDAALKVVRAVLGDELEIVEEQAGYSLWKKRGVTIEDETIERCREADAMLFGACTTPEDPE
AKSPIVTLRKELGLYANVRPARSWPVPRPVDTEFDLVIVRENTEGLYTGCECEIHDGVTVALRKISEEGTRRVAEVACDL
AEERSGRVTIVHKANVLKLTCGTFKRVAAETVERRGLEWDDEYVDAAAYKLVREPDSFDVILTSNLFGDILSDLAAGLMG
SLGLAPSANLGDDAALFEPVHGSAPDIAGKGIANPVAAILSAAMMLDHLGYGEEARIIERAVEEVLREGVRTPDLGGSAT
TEEVAEAIAERVATG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate, which decarboxylates to 4-methyl-2-oxopentanoate (2-oxoisocaproate). Also catalyzes the oxidative decarboxylation of 3-methylmal

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=40.2985074626866, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI4758582, Length=335, Percent_Identity=38.2089552238806, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI28178838, Length=317, Percent_Identity=38.801261829653, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI28178816, Length=333, Percent_Identity=34.5345345345345, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI28178821, Length=339, Percent_Identity=33.6283185840708, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI28178819, Length=203, Percent_Identity=34.4827586206897, Blast_Score=111, Evalue=7e-25,
Organism=Escherichia coli, GI1788101, Length=357, Percent_Identity=37.5350140056022, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI87081683, Length=364, Percent_Identity=37.6373626373626, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1787381, Length=372, Percent_Identity=32.7956989247312, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71986051, Length=334, Percent_Identity=38.622754491018, Blast_Score=215, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17550882, Length=342, Percent_Identity=35.9649122807018, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17505779, Length=331, Percent_Identity=34.1389728096677, Blast_Score=170, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI25144293, Length=340, Percent_Identity=34.7058823529412, Blast_Score=170, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6322097, Length=351, Percent_Identity=43.8746438746439, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324709, Length=335, Percent_Identity=40.2985074626866, Blast_Score=206, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324291, Length=339, Percent_Identity=36.8731563421829, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=37.465564738292, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24643268, Length=335, Percent_Identity=41.4925373134328, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24643270, Length=330, Percent_Identity=42.1212121212121, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24661184, Length=337, Percent_Identity=37.0919881305638, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI161078633, Length=328, Percent_Identity=33.8414634146341, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24650122, Length=328, Percent_Identity=33.8414634146341, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI161078635, Length=328, Percent_Identity=33.8414634146341, Blast_Score=174, Evalue=5e-44,
Organism=Drosophila melanogaster, GI161078637, Length=328, Percent_Identity=33.8414634146341, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI161078639, Length=326, Percent_Identity=33.7423312883436, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI281362242, Length=334, Percent_Identity=33.8323353293413, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24648872, Length=334, Percent_Identity=33.8323353293413, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI20130355, Length=329, Percent_Identity=27.6595744680851, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45550436, Length=256, Percent_Identity=25.78125, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85; =1.1.1.83 [H]

Molecular weight: Translated: 35909; Mature: 35909

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASPTVVVIPGDGIGPEVIDAALKVVRAVLGDELEIVEEQAGYSLWKKRGVTIEDETIE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
RCREADAMLFGACTTPEDPEAKSPIVTLRKELGLYANVRPARSWPVPRPVDTEFDLVIVR
HHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCEEEEE
ENTEGLYTGCECEIHDGVTVALRKISEEGTRRVAEVACDLAEERSGRVTIVHKANVLKLT
ECCCCCEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEE
CGTFKRVAAETVERRGLEWDDEYVDAAAYKLVREPDSFDVILTSNLFGDILSDLAAGLMG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHH
SLGLAPSANLGDDAALFEPVHGSAPDIAGKGIANPVAAILSAAMMLDHLGYGEEARIIER
HCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
AVEEVLREGVRTPDLGGSATTEEVAEAIAERVATG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNASPTVVVIPGDGIGPEVIDAALKVVRAVLGDELEIVEEQAGYSLWKKRGVTIEDETIE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
RCREADAMLFGACTTPEDPEAKSPIVTLRKELGLYANVRPARSWPVPRPVDTEFDLVIVR
HHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCEEEEE
ENTEGLYTGCECEIHDGVTVALRKISEEGTRRVAEVACDLAEERSGRVTIVHKANVLKLT
ECCCCCEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEE
CGTFKRVAAETVERRGLEWDDEYVDAAAYKLVREPDSFDVILTSNLFGDILSDLAAGLMG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHH
SLGLAPSANLGDDAALFEPVHGSAPDIAGKGIANPVAAILSAAMMLDHLGYGEEARIIER
HCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
AVEEVLREGVRTPDLGGSATTEEVAEAIAERVATG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]