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The map label for this gene is HyuB

Identifier: 20094484

GI number: 20094484

Start: 1015395

End: 1016936

Strand: Direct

Name: HyuB

Synonym: MK1048

Alternate gene names: NA

Gene position: 1015395-1016936 (Clockwise)

Preceding gene: 20094483

Following gene: 20094485

Centisome position: 59.91

GC content: 66.54

Gene sequence:

>1542_bases
ATGAAGCATGAGATCGTCCGGCAGGCTCTCATCTACGCCTGCGAGGAGATGGGGGCCCTGCTCCGCCGTAGCGCCTTCTC
CCCGAACATCCGCGAGCGGGAGGATTTCTCCTGCGCGATCTACGACGCGGACGGTGAGCTGATAGCCCAGGCGGAGCACA
TTCCGGTGCATCTCGGATCGATGAGGTGGGCCGTACAGGCCGTCCTCGATGAGTTCGGGTCCTCCGAGATGGAACCGGGA
GACGCCTTCCTGCTCAACGACCCGTACGCCGGAGGTACCCATCTCCCCGATCTCACCGTCGTCGCTCCCGCGTTCCACGA
CGATGAGCTCGTGGCGTTCGCCGCAGTTCGTGCGCACCACGCCGACGTAGGTGGGAAGGTCCCAGGGAGCATGCCTCACG
ACGCCGAGGACGTGTACTCGGAGGGGCTCCGGATTCCCCCGATCAAGGTCGCCGAGGACGGTGAGCCCCGAGACGACGTG
CTACGCCTCCTCGAGGCGAACTCCCGGGGCGGTGAGGAGCGGCGGTACGACCTCACCGCTCAGATCGCCGCGGCGTTCCG
AGGATGCCGCCAGGTGAGGAGGATCATCGACGAGCACGGACGGGAAGCCTGGGAGAGGGCCTGCGAGTGGTGTAAGGACT
ACGCCGAGCGTCGCATGCGCGTGGCCATCGAGCGGATCCCCGACGGCGAGTACAGCGGGGAGGATTACCTGGAGGGCGAC
GGGATCTCGGAGGACCCCGTGCGTATCTCCGTGACCGTGCGCGTGGAGGGGGACGAGGTGACCGTCGACTTCACCGGGAC
CGACGAGCAGACTCGGGGTCCCGTGAACGCGCCTCTGCCGGTCACGTACTCCGCGGTCTTCTTCGCCCTCAAGGCGGCCC
TGGACCCGGAGACACCCGTTTCAGAGGGGACGTACAGGCCTATCGAGGTGATCGCGCCACGCGGAACGGTGGTCAACCCC
GAGCCGCCGGCCCCGGTATGCGCGGGCAACGTGGAGACTTCCCAACGGATCGTCGATGCCATCCTGGATGCACTACGTGA
GGCGATCCCTAGCCTACCGGCCCACTCGCACGGCTCCATGAACAACGTGGCCATCGGCGGGGTCGGATTCGCGTACTACG
AGACCGTGGGCGGTGGGGCCGGCGCATCCCCGGACTGTGACGGGGAGAGCGCCGTGCACGTGTACATGACCAACACCGCG
AACACTCCGGTCGAGCACGTGGAGCGCGAGTATCCGATCCGGATCCTCGAGCATACCGTCCGGCGTGGGAGTGGGGGCGA
GGGTAAGTACCGCGGCGGCGACGGTATCATCAAGCGATACCTCGCCCTGGAGCGCTGTCGTGTGACCGTGATAGGCGACC
GGACCATGCACCCGCCGCGCGGCGTCGACGGAGGGAAACCGGGGAAGACCTCCGAGTACGAAGTCGAGCGATCGAACGGG
AAGGTAGAGAGGCTAGGTCCGAAGGACTCGACCGTGCTCGAACCCGGTGACATGTTGATCGTGCGCACCGCTGGCGGTGG
AGGTTACGGAAAGGGGGAGTGA

Upstream 100 bases:

>100_bases
GACGTGTTGCTCCACGTGGGGCCGGGGGTGGTGAACGCGTACGATCGGGTCAAGCGCGCCTTCCTGCGCGGTCTGACCTC
GGTGCTGGGGGAACCTTCGG

Downstream 100 bases:

>100_bases
ACGGGGAATGGAGATCTCGGAGTTTCAGCGTCTGATGGACGAGCTGTACGGGGAGAAGGATCGGAAGCGCGGTCCCGAAC
GGACGTTGCTATGGCTCGTG

Product: N-methylhydantoinase B

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKHEIVRQALIYACEEMGALLRRSAFSPNIREREDFSCAIYDADGELIAQAEHIPVHLGSMRWAVQAVLDEFGSSEMEPG
DAFLLNDPYAGGTHLPDLTVVAPAFHDDELVAFAAVRAHHADVGGKVPGSMPHDAEDVYSEGLRIPPIKVAEDGEPRDDV
LRLLEANSRGGEERRYDLTAQIAAAFRGCRQVRRIIDEHGREAWERACEWCKDYAERRMRVAIERIPDGEYSGEDYLEGD
GISEDPVRISVTVRVEGDEVTVDFTGTDEQTRGPVNAPLPVTYSAVFFALKAALDPETPVSEGTYRPIEVIAPRGTVVNP
EPPAPVCAGNVETSQRIVDAILDALREAIPSLPAHSHGSMNNVAIGGVGFAYYETVGGGAGASPDCDGESAVHVYMTNTA
NTPVEHVEREYPIRILEHTVRRGSGGEGKYRGGDGIIKRYLALERCRVTVIGDRTMHPPRGVDGGKPGKTSEYEVERSNG
KVERLGPKDSTVLEPGDMLIVRTAGGGGYGKGE

Sequences:

>Translated_513_residues
MKHEIVRQALIYACEEMGALLRRSAFSPNIREREDFSCAIYDADGELIAQAEHIPVHLGSMRWAVQAVLDEFGSSEMEPG
DAFLLNDPYAGGTHLPDLTVVAPAFHDDELVAFAAVRAHHADVGGKVPGSMPHDAEDVYSEGLRIPPIKVAEDGEPRDDV
LRLLEANSRGGEERRYDLTAQIAAAFRGCRQVRRIIDEHGREAWERACEWCKDYAERRMRVAIERIPDGEYSGEDYLEGD
GISEDPVRISVTVRVEGDEVTVDFTGTDEQTRGPVNAPLPVTYSAVFFALKAALDPETPVSEGTYRPIEVIAPRGTVVNP
EPPAPVCAGNVETSQRIVDAILDALREAIPSLPAHSHGSMNNVAIGGVGFAYYETVGGGAGASPDCDGESAVHVYMTNTA
NTPVEHVEREYPIRILEHTVRRGSGGEGKYRGGDGIIKRYLALERCRVTVIGDRTMHPPRGVDGGKPGKTSEYEVERSNG
KVERLGPKDSTVLEPGDMLIVRTAGGGGYGKGE
>Mature_513_residues
MKHEIVRQALIYACEEMGALLRRSAFSPNIREREDFSCAIYDADGELIAQAEHIPVHLGSMRWAVQAVLDEFGSSEMEPG
DAFLLNDPYAGGTHLPDLTVVAPAFHDDELVAFAAVRAHHADVGGKVPGSMPHDAEDVYSEGLRIPPIKVAEDGEPRDDV
LRLLEANSRGGEERRYDLTAQIAAAFRGCRQVRRIIDEHGREAWERACEWCKDYAERRMRVAIERIPDGEYSGEDYLEGD
GISEDPVRISVTVRVEGDEVTVDFTGTDEQTRGPVNAPLPVTYSAVFFALKAALDPETPVSEGTYRPIEVIAPRGTVVNP
EPPAPVCAGNVETSQRIVDAILDALREAIPSLPAHSHGSMNNVAIGGVGFAYYETVGGGAGASPDCDGESAVHVYMTNTA
NTPVEHVEREYPIRILEHTVRRGSGGEGKYRGGDGIIKRYLALERCRVTVIGDRTMHPPRGVDGGKPGKTSEYEVERSNG
KVERLGPKDSTVLEPGDMLIVRTAGGGGYGKGE

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=532, Percent_Identity=38.3458646616541, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI133901900, Length=530, Percent_Identity=36.9811320754717, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI133901902, Length=502, Percent_Identity=37.0517928286853, Blast_Score=311, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6322634, Length=530, Percent_Identity=34.9056603773585, Blast_Score=325, Evalue=8e-90,
Organism=Drosophila melanogaster, GI45550492, Length=522, Percent_Identity=36.7816091954023, Blast_Score=306, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 55710; Mature: 55710

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHEIVRQALIYACEEMGALLRRSAFSPNIREREDFSCAIYDADGELIAQAEHIPVHLGS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCCEEECH
MRWAVQAVLDEFGSSEMEPGDAFLLNDPYAGGTHLPDLTVVAPAFHDDELVAFAAVRAHH
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
ADVGGKVPGSMPHDAEDVYSEGLRIPPIKVAEDGEPRDDVLRLLEANSRGGEERRYDLTA
CCCCCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
QIAAAFRGCRQVRRIIDEHGREAWERACEWCKDYAERRMRVAIERIPDGEYSGEDYLEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GISEDPVRISVTVRVEGDEVTVDFTGTDEQTRGPVNAPLPVTYSAVFFALKAALDPETPV
CCCCCCEEEEEEEEECCCEEEEEEECCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
SEGTYRPIEVIAPRGTVVNPEPPAPVCAGNVETSQRIVDAILDALREAIPSLPAHSHGSM
CCCCCCCEEEECCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NNVAIGGVGFAYYETVGGGAGASPDCDGESAVHVYMTNTANTPVEHVEREYPIRILEHTV
CCEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCHHHHHHHH
RRGSGGEGKYRGGDGIIKRYLALERCRVTVIGDRTMHPPRGVDGGKPGKTSEYEVERSNG
HCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCC
KVERLGPKDSTVLEPGDMLIVRTAGGGGYGKGE
CEEECCCCCCCEECCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKHEIVRQALIYACEEMGALLRRSAFSPNIREREDFSCAIYDADGELIAQAEHIPVHLGS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCCEEECH
MRWAVQAVLDEFGSSEMEPGDAFLLNDPYAGGTHLPDLTVVAPAFHDDELVAFAAVRAHH
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHH
ADVGGKVPGSMPHDAEDVYSEGLRIPPIKVAEDGEPRDDVLRLLEANSRGGEERRYDLTA
CCCCCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
QIAAAFRGCRQVRRIIDEHGREAWERACEWCKDYAERRMRVAIERIPDGEYSGEDYLEGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GISEDPVRISVTVRVEGDEVTVDFTGTDEQTRGPVNAPLPVTYSAVFFALKAALDPETPV
CCCCCCEEEEEEEEECCCEEEEEEECCCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
SEGTYRPIEVIAPRGTVVNPEPPAPVCAGNVETSQRIVDAILDALREAIPSLPAHSHGSM
CCCCCCCEEEECCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NNVAIGGVGFAYYETVGGGAGASPDCDGESAVHVYMTNTANTPVEHVEREYPIRILEHTV
CCEEECCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCHHHHHHHH
RRGSGGEGKYRGGDGIIKRYLALERCRVTVIGDRTMHPPRGVDGGKPGKTSEYEVERSNG
HCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCC
KVERLGPKDSTVLEPGDMLIVRTAGGGGYGKGE
CEEECCCCCCCEECCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]