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The map label for this gene is MurD_2 [C]

Identifier: 20094483

GI number: 20094483

Start: 1013993

End: 1015405

Strand: Direct

Name: MurD_2 [C]

Synonym: MK1047

Alternate gene names: 20094483

Gene position: 1013993-1015405 (Clockwise)

Preceding gene: 20094481

Following gene: 20094484

Centisome position: 59.82

GC content: 66.31

Gene sequence:

>1413_bases
TTGAAGCGGGTTATAGTTGTCGGAGCCGGCAGTGCGGGACGGTCGGTGGCGCGGCTGCTGAACCACGTGGGGTACGACGT
CGTGATCAACGACATCCGCGATTGGGAGGACTTCACCACGGAGGAGCGCGAGTATCTGGAGGTTCTCGAGCGCGAGGGCG
TCGAGGTGGCCCTCGGGGGACACGATCGGGAGCTCTTCGAGTCGGCCGACGCGGCCTTCGTGTCCCCGGCGATCCCCGAG
GACGCCGAGGGTAGGAAGCTCGCGGAGCTGGTCCCCGAGGAGATCACCGTCGAGGACGTTGCCGGGCTGTTGGGAGACCT
GTTCCCCGTACCCGCCATCGGGATCACAGGAACGAACGGGAAGACCACCACGACCTGGGCGGTGGCGCATCTCCTGGAGG
CCTGCGGGTACGAGGTCTGGCGGTGCTCGTCCCTCGACAGGCACCTCGTCATCGAGGCGATAGTGGACGGCGTGGTGACG
GGTGAGATCGAGGGCTACGACGTGGCCGTCGTGGAGCTCCCACACGGGACGATCAGACTGGCGGCCGGTATCGAGCTGGA
CGTCGGGGTGCTGACGAACGTCTCACCGGAGCACTTGGACGAGTTCGGCGGTTCCTTCGAGCGGTACGTTCGTCGGAAAC
TCACGATAACTGAAATGAGTGAAGTGCTGGTGGCCGGCTACGACTGCGAGGAGCTGCGGAAGCGCCTGAACGACGCGGTC
TGGTACTCCGTGCGGGATCCCGACGCCGACTACTACGGACGTAGGGAGAACGGACGTCTCGTGGTGAAGGGACCCGACGC
CTCGGTGGAAGCCGACTTCCACCTGATCGGTTACTACGTAGAGAACTGCACGGGAGCCGTCGCCGCCTGTCTAGAGTTCG
GCGCCGATCCCGAGGGGGTCGCCGAGGGTCTCGCCACGTTCGAGGGCGTTCCCGGGCGCATGGAGCTGCTGGGGGAGTTC
TCGGGTCGTGTAGCCTACATCGACGCGGCACACAATCCGGACGGTCTGAAGGCGTCGCTTCCACCGTTCCGGGAGCTCGC
GGACGAACGGGGCGGAAGACTGCTCGTCAGCGTGGACAATCCCGACACCGTGACCGAGCGCGACAAGTTCGAGTTCGGGC
GGATAGTAGGGGAGTACGCCGATTTCGTGGCGGCCAGCGGGTACAACGAGACCCTGGAGCGGCTCGACCGGTCGGCCGCC
GAGGAGGTAGTGAAGGGCGCCGAGTCGGCGGGTTGCGAGGGGGTGGCCGTAGACTCGGTCCGGGAGGCTGCCGAGGAGGT
GGCCGCACGGTCCGAGGAGGGAGACGTGTTGCTCCACGTGGGGCCGGGGGTGGTGAACGCGTACGATCGGGTCAAGCGCG
CCTTCCTGCGCGGTCTGACCTCGGTGCTGGGGGAACCTTCGGATGAAGCATGA

Upstream 100 bases:

>100_bases
ACACGTAGCCGTCGACGCGACCGGCGGCACCGTTTAAGGTAACCCGGCGGGTCTCGACGGGTCTCAAGGCGGATCCCGCC
CCGCCGGGAGGGTGAACGTG

Downstream 100 bases:

>100_bases
GATCGTCCGGCAGGCTCTCATCTACGCCTGCGAGGAGATGGGGGCCCTGCTCCGCCGTAGCGCCTTCTCCCCGAACATCC
GCGAGCGGGAGGATTTCTCC

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKRVIVVGAGSAGRSVARLLNHVGYDVVINDIRDWEDFTTEEREYLEVLEREGVEVALGGHDRELFESADAAFVSPAIPE
DAEGRKLAELVPEEITVEDVAGLLGDLFPVPAIGITGTNGKTTTTWAVAHLLEACGYEVWRCSSLDRHLVIEAIVDGVVT
GEIEGYDVAVVELPHGTIRLAAGIELDVGVLTNVSPEHLDEFGGSFERYVRRKLTITEMSEVLVAGYDCEELRKRLNDAV
WYSVRDPDADYYGRRENGRLVVKGPDASVEADFHLIGYYVENCTGAVAACLEFGADPEGVAEGLATFEGVPGRMELLGEF
SGRVAYIDAAHNPDGLKASLPPFRELADERGGRLLVSVDNPDTVTERDKFEFGRIVGEYADFVAASGYNETLERLDRSAA
EEVVKGAESAGCEGVAVDSVREAAEEVAARSEEGDVLLHVGPGVVNAYDRVKRAFLRGLTSVLGEPSDEA

Sequences:

>Translated_470_residues
MKRVIVVGAGSAGRSVARLLNHVGYDVVINDIRDWEDFTTEEREYLEVLEREGVEVALGGHDRELFESADAAFVSPAIPE
DAEGRKLAELVPEEITVEDVAGLLGDLFPVPAIGITGTNGKTTTTWAVAHLLEACGYEVWRCSSLDRHLVIEAIVDGVVT
GEIEGYDVAVVELPHGTIRLAAGIELDVGVLTNVSPEHLDEFGGSFERYVRRKLTITEMSEVLVAGYDCEELRKRLNDAV
WYSVRDPDADYYGRRENGRLVVKGPDASVEADFHLIGYYVENCTGAVAACLEFGADPEGVAEGLATFEGVPGRMELLGEF
SGRVAYIDAAHNPDGLKASLPPFRELADERGGRLLVSVDNPDTVTERDKFEFGRIVGEYADFVAASGYNETLERLDRSAA
EEVVKGAESAGCEGVAVDSVREAAEEVAARSEEGDVLLHVGPGVVNAYDRVKRAFLRGLTSVLGEPSDEA
>Mature_470_residues
MKRVIVVGAGSAGRSVARLLNHVGYDVVINDIRDWEDFTTEEREYLEVLEREGVEVALGGHDRELFESADAAFVSPAIPE
DAEGRKLAELVPEEITVEDVAGLLGDLFPVPAIGITGTNGKTTTTWAVAHLLEACGYEVWRCSSLDRHLVIEAIVDGVVT
GEIEGYDVAVVELPHGTIRLAAGIELDVGVLTNVSPEHLDEFGGSFERYVRRKLTITEMSEVLVAGYDCEELRKRLNDAV
WYSVRDPDADYYGRRENGRLVVKGPDASVEADFHLIGYYVENCTGAVAACLEFGADPEGVAEGLATFEGVPGRMELLGEF
SGRVAYIDAAHNPDGLKASLPPFRELADERGGRLLVSVDNPDTVTERDKFEFGRIVGEYADFVAASGYNETLERLDRSAA
EEVVKGAESAGCEGVAVDSVREAAEEVAARSEEGDVLLHVGPGVVNAYDRVKRAFLRGLTSVLGEPSDEA

Specific function: Cell Wall Formation. Catalyzes The Addition Of Glutamate To The Nucleotide Precursor Udp-N-Acetylmuramoyl-L- Alanine (Uma). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013221
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: 6.3.2.9

Molecular weight: Translated: 50850; Mature: 50850

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVIVVGAGSAGRSVARLLNHVGYDVVINDIRDWEDFTTEEREYLEVLEREGVEVALGG
CCEEEEEECCCCHHHHHHHHHHCCHHEEECCHHCHHHCCCHHHHHHHHHHHCCCEEEECC
HDRELFESADAAFVSPAIPEDAEGRKLAELVPEEITVEDVAGLLGDLFPVPAIGITGTNG
CCHHHHHCCCCCEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCC
KTTTTWAVAHLLEACGYEVWRCSSLDRHLVIEAIVDGVVTGEIEGYDVAVVELPHGTIRL
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCEECCCCCCEEEEEECCCCEEEE
AAGIELDVGVLTNVSPEHLDEFGGSFERYVRRKLTITEMSEVLVAGYDCEELRKRLNDAV
EECCEEECHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHE
WYSVRDPDADYYGRRENGRLVVKGPDASVEADFHLIGYYVENCTGAVAACLEFGADPEGV
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCHHHH
AEGLATFEGVPGRMELLGEFSGRVAYIDAAHNPDGLKASLPPFRELADERGGRLLVSVDN
HHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEEEECC
PDTVTERDKFEFGRIVGEYADFVAASGYNETLERLDRSAAEEVVKGAESAGCEGVAVDSV
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCEEHHHH
REAAEEVAARSEEGDVLLHVGPGVVNAYDRVKRAFLRGLTSVLGEPSDEA
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRVIVVGAGSAGRSVARLLNHVGYDVVINDIRDWEDFTTEEREYLEVLEREGVEVALGG
CCEEEEEECCCCHHHHHHHHHHCCHHEEECCHHCHHHCCCHHHHHHHHHHHCCCEEEECC
HDRELFESADAAFVSPAIPEDAEGRKLAELVPEEITVEDVAGLLGDLFPVPAIGITGTNG
CCHHHHHCCCCCEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCC
KTTTTWAVAHLLEACGYEVWRCSSLDRHLVIEAIVDGVVTGEIEGYDVAVVELPHGTIRL
CCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCEECCCCCCEEEEEECCCCEEEE
AAGIELDVGVLTNVSPEHLDEFGGSFERYVRRKLTITEMSEVLVAGYDCEELRKRLNDAV
EECCEEECHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHE
WYSVRDPDADYYGRRENGRLVVKGPDASVEADFHLIGYYVENCTGAVAACLEFGADPEGV
EEEECCCCCCCCCCCCCCEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCHHHH
AEGLATFEGVPGRMELLGEFSGRVAYIDAAHNPDGLKASLPPFRELADERGGRLLVSVDN
HHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCEEEEEECC
PDTVTERDKFEFGRIVGEYADFVAASGYNETLERLDRSAAEEVVKGAESAGCEGVAVDSV
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCEEHHHH
REAAEEVAARSEEGDVLLHVGPGVVNAYDRVKRAFLRGLTSVLGEPSDEA
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Potassium phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {MgATP2-}} 0.03 {UDP-N-acetylmuramoyl-L-Ala}} 0.0075 {UDP-N-acetylmuramoyl-L-Ala}} 0.0055 {UDP-N-acetylmuramoyl-L-Ala}} 0.01 {dihydrouridine} 0.28 {D-Glu}} 0.13 {D-Glu}} 0.055 {D-Glu}} 0.2 {1-phospho-N-acetylmuramoyl-L-Ala}} [C

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanine; glutamate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: Pentasodium salt; 1-Phospho-N-acetyl muramoyl -L-Ala-D-Glu; 2, 4, 6-Trinitrobenzenesulfonic acid; D-2-Aminoadipic acid; D-Asp; D-erythro-3-Methyl glutamic acid; D-erythro-4-Methyl glutamic acid; D-Gln; D-Pyroglutamic acid; DL-2-Amino-4-phosphonobutyric ac

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]