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The map label for this gene is ArgG

Identifier: 20094378

GI number: 20094378

Start: 901574

End: 902758

Strand: Direct

Name: ArgG

Synonym: MK0942

Alternate gene names: 20094378

Gene position: 901574-902758 (Clockwise)

Preceding gene: 20094375

Following gene: 20094382

Centisome position: 53.19

GC content: 66.33

Gene sequence:

>1185_bases
ATGTCGCGGGTGGTTCTCGCGTATTCAGGAGGCTTGGATACCTCGGTTTGCATCGAGCTCCTGCGGGAGCGGTACGGGTA
CGACGAGGTGATCACGGTGACCGCGGACGTGGGTCAGCCGGAGGAGGAGCTGCGGGAGGCGGAGGAGAAGGCACGGAAGC
TCGCGGATGAGCACTTCACGGTCGACTGTGTGGACGAGTTCGTGTGTGAGTACTGCTGGCGAGCCGTGAAGGCGAACGCG
ACGTACGAGGGGTACCCTCTGAGCACGGCGCTGGCCCGACCGCTGATCGCGCAGAAGGTCCTGGAAGTGGCGCGTGATGT
GGACGCGGACGCGGTCGCACACGGGTGCACGGGGAAGGGCAACGACCAGTTCCGGTTCGAGTCGTATTTGCGGGCGCACG
GCGGTGAGGAGTTCGAGATCGTGGCTCCGGTTCGGGACCTGAACCTCACGCGCGACGAGGAGATCGCGTACGCCGAGGAG
CGGGGTATCCCCGTCCCGGTGGACGTCGACAGTCCCTACAGCGTGGACGAGAACCTATGGGGTCGATCGATCGAGGGCGG
CGTCCTGGAGGACCCCTCCGAGGAACCGCCGGAGGAGGTCTTCGAGTGGACCGTCAGTCCCGCGGAGGCCCCGGACGAGC
CCGAGGTCGTGGAGATCGAGTTCGAGGACGGCGTGCCCGTGGAGGTGGACGGGCGGGACGACCCGGTCGAGATCGTCCGG
TACCTGAACGAGACCGCGGGCGAGCACGGCGTGGGTCGCATCGATATCATCGAGGACCGGGTGATCGGGCTGAAGTCACG
CGAGGTGTACGAGGCACCGGCGGCGGTGACGCTGCTCGAGGCGCACCGGGCGCTGGAGGCGTTCACCCTGACACGCCGGG
AGCTGTCGCTGAAGGCCTCGCTGGAGGAGGAGTGGGCGCGACTCGTCTACGACGGGCTGTGGTTCAACCCCCTGCGCGAG
CACCTGGAGGCCTTCTTCGACTCGACGCAGCGGCACGTGGAGGGGACCGTGCGCGTGAAGCTGTTCAAGGGCTCCGCGAC
CGTGATCTCACGGGAGTCCCCGCGAGCGCTCTACGAGGAGGAGTTGATATCGTACGAAGAAAAGCAGTTCGATCAGCGGA
CGGCCGAAGGAGCCGTGAAGCTTCACGGACTCCAGGAACGGTTGGCTTTAAGGCGTCGTGGGTGA

Upstream 100 bases:

>100_bases
CGGGAATCACGCCGCAGCAGGATTCGAAACAGGAGGGCTCCCCCCACGGAGCGGTGGACGTTTTAACGTCCGAGGTCACG
GGGCGCCGGGGGTGCGTGCG

Downstream 100 bases:

>100_bases
GGCTCGGCGCGCTCCGTTCCGGTCATCCCCGCGGTCCCCGATGCCGCCTTCCTCATCCGAACGTCACCCGGCGTTACAGG
TGCTCCCGTATCCTGTCGTA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSRVVLAYSGGLDTSVCIELLRERYGYDEVITVTADVGQPEEELREAEEKARKLADEHFTVDCVDEFVCEYCWRAVKANA
TYEGYPLSTALARPLIAQKVLEVARDVDADAVAHGCTGKGNDQFRFESYLRAHGGEEFEIVAPVRDLNLTRDEEIAYAEE
RGIPVPVDVDSPYSVDENLWGRSIEGGVLEDPSEEPPEEVFEWTVSPAEAPDEPEVVEIEFEDGVPVEVDGRDDPVEIVR
YLNETAGEHGVGRIDIIEDRVIGLKSREVYEAPAAVTLLEAHRALEAFTLTRRELSLKASLEEEWARLVYDGLWFNPLRE
HLEAFFDSTQRHVEGTVRVKLFKGSATVISRESPRALYEEELISYEEKQFDQRTAEGAVKLHGLQERLALRRRG

Sequences:

>Translated_394_residues
MSRVVLAYSGGLDTSVCIELLRERYGYDEVITVTADVGQPEEELREAEEKARKLADEHFTVDCVDEFVCEYCWRAVKANA
TYEGYPLSTALARPLIAQKVLEVARDVDADAVAHGCTGKGNDQFRFESYLRAHGGEEFEIVAPVRDLNLTRDEEIAYAEE
RGIPVPVDVDSPYSVDENLWGRSIEGGVLEDPSEEPPEEVFEWTVSPAEAPDEPEVVEIEFEDGVPVEVDGRDDPVEIVR
YLNETAGEHGVGRIDIIEDRVIGLKSREVYEAPAAVTLLEAHRALEAFTLTRRELSLKASLEEEWARLVYDGLWFNPLRE
HLEAFFDSTQRHVEGTVRVKLFKGSATVISRESPRALYEEELISYEEKQFDQRTAEGAVKLHGLQERLALRRRG
>Mature_393_residues
SRVVLAYSGGLDTSVCIELLRERYGYDEVITVTADVGQPEEELREAEEKARKLADEHFTVDCVDEFVCEYCWRAVKANAT
YEGYPLSTALARPLIAQKVLEVARDVDADAVAHGCTGKGNDQFRFESYLRAHGGEEFEIVAPVRDLNLTRDEEIAYAEER
GIPVPVDVDSPYSVDENLWGRSIEGGVLEDPSEEPPEEVFEWTVSPAEAPDEPEVVEIEFEDGVPVEVDGRDDPVEIVRY
LNETAGEHGVGRIDIIEDRVIGLKSREVYEAPAAVTLLEAHRALEAFTLTRRELSLKASLEEEWARLVYDGLWFNPLREH
LEAFFDSTQRHVEGTVRVKLFKGSATVISRESPRALYEEELISYEEKQFDQRTAEGAVKLHGLQERLALRRRG

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=396, Percent_Identity=42.1717171717172, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI16950633, Length=396, Percent_Identity=42.1717171717172, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1789563, Length=396, Percent_Identity=28.2828282828283, Blast_Score=111, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=391, Percent_Identity=39.1304347826087, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI21358151, Length=411, Percent_Identity=40.3892944038929, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_METKA (Q8TWU0)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614225.1
- ProteinModelPortal:   Q8TWU0
- SMR:   Q8TWU0
- GeneID:   1477043
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0942
- NMPDR:   fig|190192.1.peg.938
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- BioCyc:   MKAN190192:MK0942-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44476; Mature: 44345

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 35-35 BINDING 85-85 BINDING 115-115 BINDING 117-117 BINDING 121-121 BINDING 121-121 BINDING 122-122 BINDING 125-125 BINDING 174-174 BINDING 183-183 BINDING 258-258 BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVVLAYSGGLDTSVCIELLRERYGYDEVITVTADVGQPEEELREAEEKARKLADEHFT
CCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
VDCVDEFVCEYCWRAVKANATYEGYPLSTALARPLIAQKVLEVARDVDADAVAHGCTGKG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
NDQFRFESYLRAHGGEEFEIVAPVRDLNLTRDEEIAYAEERGIPVPVDVDSPYSVDENLW
CCCHHHHHHHHHCCCCCEEEEECHHHCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCC
GRSIEGGVLEDPSEEPPEEVFEWTVSPAEAPDEPEVVEIEFEDGVPVEVDGRDDPVEIVR
CCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHH
YLNETAGEHGVGRIDIIEDRVIGLKSREVYEAPAAVTLLEAHRALEAFTLTRRELSLKAS
HHHHHCCCCCCCEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEEWARLVYDGLWFNPLREHLEAFFDSTQRHVEGTVRVKLFKGSATVISRESPRALYEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCHHHHHH
ELISYEEKQFDQRTAEGAVKLHGLQERLALRRRG
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRVVLAYSGGLDTSVCIELLRERYGYDEVITVTADVGQPEEELREAEEKARKLADEHFT
CEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
VDCVDEFVCEYCWRAVKANATYEGYPLSTALARPLIAQKVLEVARDVDADAVAHGCTGKG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
NDQFRFESYLRAHGGEEFEIVAPVRDLNLTRDEEIAYAEERGIPVPVDVDSPYSVDENLW
CCCHHHHHHHHHCCCCCEEEEECHHHCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCC
GRSIEGGVLEDPSEEPPEEVFEWTVSPAEAPDEPEVVEIEFEDGVPVEVDGRDDPVEIVR
CCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHH
YLNETAGEHGVGRIDIIEDRVIGLKSREVYEAPAAVTLLEAHRALEAFTLTRRELSLKAS
HHHHHCCCCCCCEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEEWARLVYDGLWFNPLREHLEAFFDSTQRHVEGTVRVKLFKGSATVISRESPRALYEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCHHHHHH
ELISYEEKQFDQRTAEGAVKLHGLQERLALRRRG
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014