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The map label for this gene is ybfF [C]

Identifier: 20094316

GI number: 20094316

Start: 830924

End: 831646

Strand: Direct

Name: ybfF [C]

Synonym: MK0880

Alternate gene names: 20094316

Gene position: 830924-831646 (Clockwise)

Preceding gene: 20094315

Following gene: 20094317

Centisome position: 49.02

GC content: 56.85

Gene sequence:

>723_bases
ATGGTCGACGTCAGCGTGATATTGCCCACGTACAACGAGCGCGAGAACCTTCCAAGGGTGATTCCCAAGATCGAGGAGGT
AGTGGAGGAAGAAGGCTGGACCGCTGAGATTCTGGTAGTCGACGACAACTCACCCGACGGAACGGCGGAAGTGGCCCGAG
AACTTTCCAGACAGTACGGTAACATCAAGGTGATCGTGCGCGAGGAGAAGCCGGGATTAGGTCTAGCGTACAGGAGAGGG
TTCCGCGAGGCTAGGGGCGAGGTGATAGTATGTATGGACGCCGACGGTCAACACCCACCAGAGTGTCTTCCTAACATCGT
GAATCCGGTACTCGATGGGGAGTGCGACTTCGGATTAGGGTCCAGGTACGTCGAAGGGTCCGTCGTTGAGAACTTCCCGT
GGTACCGTAAGCTGAACTCTTGGGGTGCACGCGTTGTTGCACGCTTGTTCCTCAAACTTCCGTACCGCGATCCGACGAGC
GGGTTCCGTGCTATTTCCCGGAAGATACTGACCGAGAGCCGACCGTTCGTGTCCGAAGGTTTCGAGATACAGGTCGAGAC
GCTCGCGAAGGCCCACCACATGGGCTACACCGTGCAGGAGTACCCGTTCTTGTTCCGACCCAGAGAGCGGGGATCCTCTA
ACGTGAATATCCGTCAGATACTACGGTACCTCAGAGGAGTGTGGAGGATCAGGAAGGACTTGAAGCAGCGTGGGCTCCTG
TAA

Upstream 100 bases:

>100_bases
CCTACACCGTTTCACCGGTTGGATCCGGAGAAGCCCGTGCACGTTTTCAGGTCGGGTGTCCGCTCGTTCACCCTCATAGC
ATCGCTGGGGGACGGGGATC

Downstream 100 bases:

>100_bases
ACGTTTTCACATACCTGCTCCGGATGGCTCGAGGGCAGAAGATTGCGCGTTGTGGTGGTAGGAGGCGGAGCCGCCGGCGT
CGTAGCCGCTCGGACTGCCC

Product: cell wall biosynthesis glycosyltransferase

Products: GDP; dolichyl D-mannosyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MVDVSVILPTYNERENLPRVIPKIEEVVEEEGWTAEILVVDDNSPDGTAEVARELSRQYGNIKVIVREEKPGLGLAYRRG
FREARGEVIVCMDADGQHPPECLPNIVNPVLDGECDFGLGSRYVEGSVVENFPWYRKLNSWGARVVARLFLKLPYRDPTS
GFRAISRKILTESRPFVSEGFEIQVETLAKAHHMGYTVQEYPFLFRPRERGSSNVNIRQILRYLRGVWRIRKDLKQRGLL

Sequences:

>Translated_240_residues
MVDVSVILPTYNERENLPRVIPKIEEVVEEEGWTAEILVVDDNSPDGTAEVARELSRQYGNIKVIVREEKPGLGLAYRRG
FREARGEVIVCMDADGQHPPECLPNIVNPVLDGECDFGLGSRYVEGSVVENFPWYRKLNSWGARVVARLFLKLPYRDPTS
GFRAISRKILTESRPFVSEGFEIQVETLAKAHHMGYTVQEYPFLFRPRERGSSNVNIRQILRYLRGVWRIRKDLKQRGLL
>Mature_240_residues
MVDVSVILPTYNERENLPRVIPKIEEVVEEEGWTAEILVVDDNSPDGTAEVARELSRQYGNIKVIVREEKPGLGLAYRRG
FREARGEVIVCMDADGQHPPECLPNIVNPVLDGECDFGLGSRYVEGSVVENFPWYRKLNSWGARVVARLFLKLPYRDPTS
GFRAISRKILTESRPFVSEGFEIQVETLAKAHHMGYTVQEYPFLFRPRERGSSNVNIRQILRYLRGVWRIRKDLKQRGLL

Specific function: Unknown

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4503363, Length=229, Percent_Identity=35.8078602620087, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI7019323, Length=261, Percent_Identity=29.1187739463602, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1788588, Length=140, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71999402, Length=226, Percent_Identity=38.4955752212389, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325441, Length=231, Percent_Identity=36.7965367965368, Blast_Score=135, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24585265, Length=226, Percent_Identity=35.3982300884956, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24582769, Length=257, Percent_Identity=28.7937743190661, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.4.1.83

Molecular weight: Translated: 27559; Mature: 27559

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDVSVILPTYNERENLPRVIPKIEEVVEEEGWTAEILVVDDNSPDGTAEVARELSRQYG
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
NIKVIVREEKPGLGLAYRRGFREARGEVIVCMDADGQHPPECLPNIVNPVLDGECDFGLG
CEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
SRYVEGSVVENFPWYRKLNSWGARVVARLFLKLPYRDPTSGFRAISRKILTESRPFVSEG
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHCC
FEIQVETLAKAHHMGYTVQEYPFLFRPRERGSSNVNIRQILRYLRGVWRIRKDLKQRGLL
CEEEHHHHHHHHHCCCCHHHCCEEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVDVSVILPTYNERENLPRVIPKIEEVVEEEGWTAEILVVDDNSPDGTAEVARELSRQYG
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
NIKVIVREEKPGLGLAYRRGFREARGEVIVCMDADGQHPPECLPNIVNPVLDGECDFGLG
CEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
SRYVEGSVVENFPWYRKLNSWGARVVARLFLKLPYRDPTSGFRAISRKILTESRPFVSEG
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHCC
FEIQVETLAKAHHMGYTVQEYPFLFRPRERGSSNVNIRQILRYLRGVWRIRKDLKQRGLL
CEEEHHHHHHHHHCCCCHHHCCEEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPmannose; dolichyl phosphate

Specific reaction: GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]