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The map label for this gene is pheT

Identifier: 20094256

GI number: 20094256

Start: 779435

End: 781207

Strand: Direct

Name: pheT

Synonym: MK0820

Alternate gene names: 20094256

Gene position: 779435-781207 (Clockwise)

Preceding gene: 20094253

Following gene: 20094257

Centisome position: 45.99

GC content: 60.58

Gene sequence:

>1773_bases
TTGCCCGTGGTCACGATCCACTACGATAAGCTCGTGAAAATACTCGGAAGGGAAGTCTCGTTCGAGGAGCTAGCTCATAA
TCTCATCCCGATGCTCGGTAGCGATGTGGAGCGAATCGACGAGCGTGAAATGGTTATCGAGACAGAGTTCTTTCCGAACC
GTCCCGACCTGTACAGCGTGGAGGGTGTTGCGCGGGCACTCAAGGGATTTCTGGGGATAGAGACGGGCATACCTGAGTAC
AACGTTCGTCGCTCGGACGTGGAGGCACGGGTCGAAGAGAGCGTACTCGACGCGCGCCCCTGCCTTGCGGTCGCCGTCGT
CCGCGGCGTCGAGTTCGAGGACGAGCGTGACTTGGAGCACCTCATGGAGTTCCAGGAGCACCTCCACTGGGTGATAGGGA
GGGACAGGAAAAAGGCTGCGATCGGTATCCACGACTTCGAAGCGGTAGAGCCACCTCTCAGGTACTTCCTCGCGGATCCG
AACGACAGGTCATGGGCGTTCGAGCCACTCGACCACCCGGGCGAAGAGATGACGCCTGCCGAGGTCCTCAGACGGCACGA
AAAGGGCCGGCAGTACGCTCACCTGGTGTCCGACGGGGCCCCGATCCTCGCGGACGAGGAGGGAGTGATCTCGTTCCCTC
CGGTGATAAACTCGGAGCGCACCAGGGTCACGCAGGACACGACGGACCTGCTCATCGACGTGACCGGTACAGATTGGCGA
TCCGTGTTGGACGCGCTTCACGTGATCGTGTGCAACCTGGCCGAACGCGGCGCGGAAATCCTTACCGTTGAGATACTGGG
GGCCTACGAGCGGACGACTCCAACGATGGAACTCGATGTATGGGACGTTCCCGTGAGCGAGGCTCGGAAACTGTTGGGGA
TCGATCTCTCCGGCGAGCAGCTGGAGGAGCTGCTGGAACGCGCTCGGCACGGGGCGATCTTCGTTCCCGAGGGTGAGCGT
GAGCTCCGGGAGTACCCGCTCCCGGATGTGTACCACATCGAGGCTGATTGGCGTGAGATCCCTCCGATACTCTACGAGGA
GGACGTGGTACGGGTCTTCGTAGGGCCCCGGCGGACGAACATCCTTCACGAGTGGGACCTGATCGAAGACGCGGGGATCA
TGTACAATTACGACCGGTTCGAGCCCACGGTACCCGACTTCTACACGCCATCACGTGCCGATAGGGAGCGCGAATTCATC
AACGTCGTGCGTGATACCCTCGCGAGGATGAAGTTCGTCGAGGTGAACTCCCTGACTCTCATCAGTCCAGAAGAGAACTA
TCGGAAAATGCGCCTCGAACCGGACGGCCGCGCGGTGAAGTTAGCTAATCCGATTCAGAAGGAGTACACGATCGTACGAA
CGTGGATCCTCCCGTCTCTGATGCGTTTCCTCGCGGACAACAAGCACCGTCCGTATCCGCAACGGGTGTTCGAGCTCGGG
GAGGTGATCGAACGGGACGAGGACGCGGAGACCGGTGCCAAGGACCGGTGGAAACTGGCGCTGGCGATCGCCGGCCCCGG
TGTGGGCTTCTCCGAGATTAAGTCCGTGGTGGAGGCACTACTGCGCGAACTGGACGTCACCGGGTGGGAGATTACCGAGA
GAAAACACCGATCATTTATTAATGGAAGGTGCGCCGCCGTACTCGCCGACGGGCGGGAGCTGGGTTTCTTCGGTGAAATT
CATCCCGAAGTACTGACTGAGTTCGACCTCGAGGTGCCCGTAGTGGGTGGGGAGTTCGACGTCGCGGCCCTTAGAACGGC
CGCCGGGTGGTGA

Upstream 100 bases:

>100_bases
TCCTCATCGGCTCGGCCACCGCCGTAGGTCCTCATCCCCGACTCGACCGGCGAGCTAACAGTATTAACGGTGTACCCGGG
ACGTTCGGGGGTGTCGGAGG

Downstream 100 bases:

>100_bases
CACTTGGAGCAGATAGTGAAGAACGCGCTGGAGCGCGTCGAACGAGAGCAGGGGGGCGACGACCCGCCGTCGTCCCGGGG
GACCGACGACGATCAGGAGT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MPVVTIHYDKLVKILGREVSFEELAHNLIPMLGSDVERIDEREMVIETEFFPNRPDLYSVEGVARALKGFLGIETGIPEY
NVRRSDVEARVEESVLDARPCLAVAVVRGVEFEDERDLEHLMEFQEHLHWVIGRDRKKAAIGIHDFEAVEPPLRYFLADP
NDRSWAFEPLDHPGEEMTPAEVLRRHEKGRQYAHLVSDGAPILADEEGVISFPPVINSERTRVTQDTTDLLIDVTGTDWR
SVLDALHVIVCNLAERGAEILTVEILGAYERTTPTMELDVWDVPVSEARKLLGIDLSGEQLEELLERARHGAIFVPEGER
ELREYPLPDVYHIEADWREIPPILYEEDVVRVFVGPRRTNILHEWDLIEDAGIMYNYDRFEPTVPDFYTPSRADREREFI
NVVRDTLARMKFVEVNSLTLISPEENYRKMRLEPDGRAVKLANPIQKEYTIVRTWILPSLMRFLADNKHRPYPQRVFELG
EVIERDEDAETGAKDRWKLALAIAGPGVGFSEIKSVVEALLRELDVTGWEITERKHRSFINGRCAAVLADGRELGFFGEI
HPEVLTEFDLEVPVVGGEFDVAALRTAAGW

Sequences:

>Translated_590_residues
MPVVTIHYDKLVKILGREVSFEELAHNLIPMLGSDVERIDEREMVIETEFFPNRPDLYSVEGVARALKGFLGIETGIPEY
NVRRSDVEARVEESVLDARPCLAVAVVRGVEFEDERDLEHLMEFQEHLHWVIGRDRKKAAIGIHDFEAVEPPLRYFLADP
NDRSWAFEPLDHPGEEMTPAEVLRRHEKGRQYAHLVSDGAPILADEEGVISFPPVINSERTRVTQDTTDLLIDVTGTDWR
SVLDALHVIVCNLAERGAEILTVEILGAYERTTPTMELDVWDVPVSEARKLLGIDLSGEQLEELLERARHGAIFVPEGER
ELREYPLPDVYHIEADWREIPPILYEEDVVRVFVGPRRTNILHEWDLIEDAGIMYNYDRFEPTVPDFYTPSRADREREFI
NVVRDTLARMKFVEVNSLTLISPEENYRKMRLEPDGRAVKLANPIQKEYTIVRTWILPSLMRFLADNKHRPYPQRVFELG
EVIERDEDAETGAKDRWKLALAIAGPGVGFSEIKSVVEALLRELDVTGWEITERKHRSFINGRCAAVLADGRELGFFGEI
HPEVLTEFDLEVPVVGGEFDVAALRTAAGW
>Mature_589_residues
PVVTIHYDKLVKILGREVSFEELAHNLIPMLGSDVERIDEREMVIETEFFPNRPDLYSVEGVARALKGFLGIETGIPEYN
VRRSDVEARVEESVLDARPCLAVAVVRGVEFEDERDLEHLMEFQEHLHWVIGRDRKKAAIGIHDFEAVEPPLRYFLADPN
DRSWAFEPLDHPGEEMTPAEVLRRHEKGRQYAHLVSDGAPILADEEGVISFPPVINSERTRVTQDTTDLLIDVTGTDWRS
VLDALHVIVCNLAERGAEILTVEILGAYERTTPTMELDVWDVPVSEARKLLGIDLSGEQLEELLERARHGAIFVPEGERE
LREYPLPDVYHIEADWREIPPILYEEDVVRVFVGPRRTNILHEWDLIEDAGIMYNYDRFEPTVPDFYTPSRADREREFIN
VVRDTLARMKFVEVNSLTLISPEENYRKMRLEPDGRAVKLANPIQKEYTIVRTWILPSLMRFLADNKHRPYPQRVFELGE
VIERDEDAETGAKDRWKLALAIAGPGVGFSEIKSVVEALLRELDVTGWEITERKHRSFINGRCAAVLADGRELGFFGEIH
PEVLTEFDLEVPVVGGEFDVAALRTAAGW

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B5 domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=621, Percent_Identity=27.6972624798712, Blast_Score=225, Evalue=1e-58,
Organism=Homo sapiens, GI24308207, Length=186, Percent_Identity=32.7956989247312, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533403, Length=556, Percent_Identity=28.2374100719424, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323089, Length=632, Percent_Identity=25.7911392405063, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI21355211, Length=624, Percent_Identity=25.4807692307692, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_METKA (Q8TX56)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614103.1
- ProteinModelPortal:   Q8TX56
- SMR:   Q8TX56
- GeneID:   1476921
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0820
- NMPDR:   fig|190192.1.peg.816
- HOGENOM:   HBG497667
- OMA:   LLTRMCL
- ProtClustDB:   PRK09616
- BioCyc:   MKAN190192:MK0820-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00284
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR004531
- InterPro:   IPR022918
- Gene3D:   G3DSA:3.30.56.20
- SMART:   SM00873
- SMART:   SM00874
- TIGRFAMs:   TIGR00471

Pfam domain/function: PF03483 B3_4; PF03484 B5; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 67512; Mature: 67381

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS51483 B5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVTIHYDKLVKILGREVSFEELAHNLIPMLGSDVERIDEREMVIETEFFPNRPDLYSV
CCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCCCCCH
EGVARALKGFLGIETGIPEYNVRRSDVEARVEESVLDARPCLAVAVVRGVEFEDERDLEH
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHH
LMEFQEHLHWVIGRDRKKAAIGIHDFEAVEPPLRYFLADPNDRSWAFEPLDHPGEEMTPA
HHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCHHHEECCCCCCCEEECCCCCCCCCCCHH
EVLRRHEKGRQYAHLVSDGAPILADEEGVISFPPVINSERTRVTQDTTDLLIDVTGTDWR
HHHHHHHHCCHHHHHHCCCCCEEECCCCCEECCCCCCCCCCEEECCCCEEEEEECCCCHH
SVLDALHVIVCNLAERGAEILTVEILGAYERTTPTMELDVWDVPVSEARKLLGIDLSGEQ
HHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCEEEEEECCCHHHHHHHHCCCCCHHH
LEELLERARHGAIFVPEGERELREYPLPDVYHIEADWREIPPILYEEDVVRVFVGPRRTN
HHHHHHHHHCCEEEECCCHHHHHHCCCCCEEEECCCHHHCCCCCCCCCEEEEEECCCCCC
ILHEWDLIEDAGIMYNYDRFEPTVPDFYTPSRADREREFINVVRDTLARMKFVEVNSLTL
CEECCCCHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCEEE
ISPEENYRKMRLEPDGRAVKLANPIQKEYTIVRTWILPSLMRFLADNKHRPYPQRVFELG
ECCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EVIERDEDAETGAKDRWKLALAIAGPGVGFSEIKSVVEALLRELDVTGWEITERKHRSFI
HHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
NGRCAAVLADGRELGFFGEIHPEVLTEFDLEVPVVGGEFDVAALRTAAGW
CCCEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure 
PVVTIHYDKLVKILGREVSFEELAHNLIPMLGSDVERIDEREMVIETEFFPNRPDLYSV
CEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCCCCCH
EGVARALKGFLGIETGIPEYNVRRSDVEARVEESVLDARPCLAVAVVRGVEFEDERDLEH
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHH
LMEFQEHLHWVIGRDRKKAAIGIHDFEAVEPPLRYFLADPNDRSWAFEPLDHPGEEMTPA
HHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCHHHEECCCCCCCEEECCCCCCCCCCCHH
EVLRRHEKGRQYAHLVSDGAPILADEEGVISFPPVINSERTRVTQDTTDLLIDVTGTDWR
HHHHHHHHCCHHHHHHCCCCCEEECCCCCEECCCCCCCCCCEEECCCCEEEEEECCCCHH
SVLDALHVIVCNLAERGAEILTVEILGAYERTTPTMELDVWDVPVSEARKLLGIDLSGEQ
HHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCEEEEEECCCHHHHHHHHCCCCCHHH
LEELLERARHGAIFVPEGERELREYPLPDVYHIEADWREIPPILYEEDVVRVFVGPRRTN
HHHHHHHHHCCEEEECCCHHHHHHCCCCCEEEECCCHHHCCCCCCCCCEEEEEECCCCCC
ILHEWDLIEDAGIMYNYDRFEPTVPDFYTPSRADREREFINVVRDTLARMKFVEVNSLTL
CEECCCCHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCEEE
ISPEENYRKMRLEPDGRAVKLANPIQKEYTIVRTWILPSLMRFLADNKHRPYPQRVFELG
ECCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EVIERDEDAETGAKDRWKLALAIAGPGVGFSEIKSVVEALLRELDVTGWEITERKHRSFI
HHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
NGRCAAVLADGRELGFFGEIHPEVLTEFDLEVPVVGGEFDVAALRTAAGW
CCCEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014