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The map label for this gene is ThiI_1 [H]

Identifier: 20094215

GI number: 20094215

Start: 739816

End: 740862

Strand: Direct

Name: ThiI_1 [H]

Synonym: MK0778

Alternate gene names: 20094215

Gene position: 739816-740862 (Clockwise)

Preceding gene: 20094208

Following gene: 20094216

Centisome position: 43.65

GC content: 60.17

Gene sequence:

>1047_bases
ATGAAGCCGGTGACCTTGATTAGGCCCTCAGGTGAGGTGACGGCTAAGAGCGCGCCCGTCCGAAAGACGTTACTGGGTGA
ACTGGCGCGGCGGTTAGAGCGGAGGCTCGGCGAGGTGGTCCGGGTACGCGGGCCGAGGCTAATAGTCCCGGGCCACCATC
CGAACGCCGCTTCGGAGTTCGGTGTCGAAGGTGCGCTACCGGGATACAAGGTGAGACCGAAACCGGCCACAATACTCAAA
GCCACGCGACGCGCGTTGCGGGAGTGCCCAGAGGAGATCAGGGTGGACGTCCGGTCACACCACGACGGCGTTTCGGGACA
TGCGCTGTTCGAGTCCGTGAGGGAGATCGTCGAGTCTACCGGCCGTAAGACTAGCAAGCGTGGGTCCCGACTGCTGGTGG
AGGTCTATCCCGACGTCGCTTACGTCTGCGGACCCGAGAAAGCCGGTCCCGGTGGTCTTCCTGTGGGCACGCAGGGGCGT
GCGCTGTGCTTACTTTCTGGAGGCTTCGACAGTCCCGTGGCCACGTGGATGGTTATGCGTAGAGGACTCGAGTGCAAAGG
GTTGCACTTTTTAGTCACGGAGTCGGAGCTTGAAGCGGCCCGTGAGAACGAGCGAGTCCTCCGACGCTGGTGCCCGGACT
TCGATCTCCTAGTGGACGAGTCGCACCGTGAGTTCCTGGAGACCGCCGAGGAGCGACTGACCGGACGACTCAAACGATAC
CTGTGCGTTGTTTGCAAGATGAGGATGCTCGAGCGTGCCTCTCAATGGGCCGATCGGCTCGGGTGTGATTGTATTGTCAC
CGGTGACTCATTGGGTCAAGTGGCGAGTCAAACCGTATGGAACCTCAAGCTGGAGGAGTCTCAAGTGAATGGTATCGTAC
TACGTCCGTTGGTCGGTCTGAACAAACCCGAGATAGTCCGATACGGACGTAGGATAGGGGTCCCTGAACGCGATCCGGGA
CGATGTCCGTTCGTTCCGGAGAGACTTATCGTTAAACCCAGGAAACGGGAGGCGTTGCGCGCGTATCGGGAGGTGATTAA
GGGATGA

Upstream 100 bases:

>100_bases
ACCCGCCCGTAAAACGGCCTTCGGGGGCGATGAGGACACCCGAGGCCTCGGGGAACGATGACCGGAGCGGTGGGCGGCCG
AGCTTGTAGGGGGTAGTTCC

Downstream 100 bases:

>100_bases
TCATTAACTCCGTCGGTAGAAAGGTGCTCTAACGTCTGAGAATTTGAGGGGTGGTGTCCATAGGCTTTGAGAGAAGAAGT
CGCGTCCAGCCCTCAAGGGC

Product: thiamine biosynthesis ATP pyrophosphatase

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKPVTLIRPSGEVTAKSAPVRKTLLGELARRLERRLGEVVRVRGPRLIVPGHHPNAASEFGVEGALPGYKVRPKPATILK
ATRRALRECPEEIRVDVRSHHDGVSGHALFESVREIVESTGRKTSKRGSRLLVEVYPDVAYVCGPEKAGPGGLPVGTQGR
ALCLLSGGFDSPVATWMVMRRGLECKGLHFLVTESELEAARENERVLRRWCPDFDLLVDESHREFLETAEERLTGRLKRY
LCVVCKMRMLERASQWADRLGCDCIVTGDSLGQVASQTVWNLKLEESQVNGIVLRPLVGLNKPEIVRYGRRIGVPERDPG
RCPFVPERLIVKPRKREALRAYREVIKG

Sequences:

>Translated_348_residues
MKPVTLIRPSGEVTAKSAPVRKTLLGELARRLERRLGEVVRVRGPRLIVPGHHPNAASEFGVEGALPGYKVRPKPATILK
ATRRALRECPEEIRVDVRSHHDGVSGHALFESVREIVESTGRKTSKRGSRLLVEVYPDVAYVCGPEKAGPGGLPVGTQGR
ALCLLSGGFDSPVATWMVMRRGLECKGLHFLVTESELEAARENERVLRRWCPDFDLLVDESHREFLETAEERLTGRLKRY
LCVVCKMRMLERASQWADRLGCDCIVTGDSLGQVASQTVWNLKLEESQVNGIVLRPLVGLNKPEIVRYGRRIGVPERDPG
RCPFVPERLIVKPRKREALRAYREVIKG
>Mature_348_residues
MKPVTLIRPSGEVTAKSAPVRKTLLGELARRLERRLGEVVRVRGPRLIVPGHHPNAASEFGVEGALPGYKVRPKPATILK
ATRRALRECPEEIRVDVRSHHDGVSGHALFESVREIVESTGRKTSKRGSRLLVEVYPDVAYVCGPEKAGPGGLPVGTQGR
ALCLLSGGFDSPVATWMVMRRGLECKGLHFLVTESELEAARENERVLRRWCPDFDLLVDESHREFLETAEERLTGRLKRY
LCVVCKMRMLERASQWADRLGCDCIVTGDSLGQVASQTVWNLKLEESQVNGIVLRPLVGLNKPEIVRYGRRIGVPERDPG
RCPFVPERLIVKPRKREALRAYREVIKG

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=208, Percent_Identity=31.7307692307692, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 38941; Mature: 38941

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVTLIRPSGEVTAKSAPVRKTLLGELARRLERRLGEVVRVRGPRLIVPGHHPNAASEF
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHC
GVEGALPGYKVRPKPATILKATRRALRECPEEIRVDVRSHHDGVSGHALFESVREIVEST
CCCCCCCCCEECCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GRKTSKRGSRLLVEVYPDVAYVCGPEKAGPGGLPVGTQGRALCLLSGGFDSPVATWMVMR
CCCCHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
RGLECKGLHFLVTESELEAARENERVLRRWCPDFDLLVDESHREFLETAEERLTGRLKRY
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHHH
LCVVCKMRMLERASQWADRLGCDCIVTGDSLGQVASQTVWNLKLEESQVNGIVLRPLVGL
HHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHEEEECCCCCCCEEEECCCCC
NKPEIVRYGRRIGVPERDPGRCPFVPERLIVKPRKREALRAYREVIKG
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPVTLIRPSGEVTAKSAPVRKTLLGELARRLERRLGEVVRVRGPRLIVPGHHPNAASEF
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHC
GVEGALPGYKVRPKPATILKATRRALRECPEEIRVDVRSHHDGVSGHALFESVREIVEST
CCCCCCCCCEECCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GRKTSKRGSRLLVEVYPDVAYVCGPEKAGPGGLPVGTQGRALCLLSGGFDSPVATWMVMR
CCCCHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
RGLECKGLHFLVTESELEAARENERVLRRWCPDFDLLVDESHREFLETAEERLTGRLKRY
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHHHHHHHHHHHHHHHH
LCVVCKMRMLERASQWADRLGCDCIVTGDSLGQVASQTVWNLKLEESQVNGIVLRPLVGL
HHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHEEEECCCCCCCEEEECCCCC
NKPEIVRYGRRIGVPERDPGRCPFVPERLIVKPRKREALRAYREVIKG
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]