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The map label for this gene is HycB [C]

Identifier: 20094162

GI number: 20094162

Start: 693426

End: 693929

Strand: Direct

Name: HycB [C]

Synonym: MK0725

Alternate gene names: 20094162

Gene position: 693426-693929 (Clockwise)

Preceding gene: 20094161

Following gene: 20094163

Centisome position: 40.91

GC content: 65.08

Gene sequence:

>504_bases
TTGCCGGACGTTTTCGTCCTGGTCGTCGCCCCGGACAGGTGCACGGACTGCCGTAAGTGCGTCAACGCGTGCGAGCGCAC
CCACGGTCACGCCCGGATTTACAAGATCTCCGAGGATGCGCCCCCCGTCACGTGTCTTCAGTGTGACGACGCACCCTGCG
CGAACGTCTGCCCGGTGAACGCGATCGTGGAGGAGGGCGACTCGTGGATCGTCACCGAGGACTGCGTCGGCTGCGGTTTG
TGCGCCGTCGCGTGCCCCTTCGGCGCCATCGAAATGGTGAACGGCCGGGCCGACAAGTGCACCCTCTGCGTGGACGCCCC
CAAGAAAGTGCCCGCGTGCGTGGAGGCGTGTGATCGGGGCGCGCTGAAGTTGGTCTCGAAGTCGCAGGTCGCGGAGGAGA
AACGCGAACGGTTCGCCATCGAGATGGAACGGATATACCGGACGCTCGCGAAGCTCGAATCGGAGGAGGGGTCCCTTCGG
GGTGAAAGTGGGAGTCGTGGGTAA

Upstream 100 bases:

>100_bases
CGTCGACCTAGAGGAGGGGATCGAGCGAACCTGGGAGTGGATCCGTCGGAAAATCGCGTAGCCCTTTAATCCCCACCTCG
GAGGTCCTGGGGGTGCCGAG

Downstream 100 bases:

>100_bases
AGGGGGCGTCGGCAAGACCACCGTAGCCGCCGCTCTCGCCAAGGCTCTCACGGACGTAGGGTACGAGGTGCTGGCCGTAG
ACGCCGATCCCGACCCCGAC

Product: Fe-S-cluster-containing hydrogenase component

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MPDVFVLVVAPDRCTDCRKCVNACERTHGHARIYKISEDAPPVTCLQCDDAPCANVCPVNAIVEEGDSWIVTEDCVGCGL
CAVACPFGAIEMVNGRADKCTLCVDAPKKVPACVEACDRGALKLVSKSQVAEEKRERFAIEMERIYRTLAKLESEEGSLR
GESGSRG

Sequences:

>Translated_167_residues
MPDVFVLVVAPDRCTDCRKCVNACERTHGHARIYKISEDAPPVTCLQCDDAPCANVCPVNAIVEEGDSWIVTEDCVGCGL
CAVACPFGAIEMVNGRADKCTLCVDAPKKVPACVEACDRGALKLVSKSQVAEEKRERFAIEMERIYRTLAKLESEEGSLR
GESGSRG
>Mature_166_residues
PDVFVLVVAPDRCTDCRKCVNACERTHGHARIYKISEDAPPVTCLQCDDAPCANVCPVNAIVEEGDSWIVTEDCVGCGLC
AVACPFGAIEMVNGRADKCTLCVDAPKKVPACVEACDRGALKLVSKSQVAEEKRERFAIEMERIYRTLAKLESEEGSLRG
ESGSRG

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082180, Length=138, Percent_Identity=36.9565217391304, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082114, Length=180, Percent_Identity=32.7777777777778, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI2367245, Length=149, Percent_Identity=37.5838926174497, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1789067, Length=170, Percent_Identity=35.2941176470588, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1788811, Length=160, Percent_Identity=35.625, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI87082179, Length=153, Percent_Identity=31.3725490196078, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1789079, Length=189, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787749, Length=109, Percent_Identity=34.8623853211009, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI2367345, Length=166, Percent_Identity=31.9277108433735, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI226510944, Length=140, Percent_Identity=32.8571428571429, Blast_Score=60, Evalue=6e-11,
Organism=Escherichia coli, GI1787122, Length=152, Percent_Identity=30.2631578947368, Blast_Score=60, Evalue=8e-11,
Organism=Escherichia coli, GI1790326, Length=109, Percent_Identity=34.8623853211009, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 18042; Mature: 17911

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
11.4 %Cys+Met (Translated Protein)
9.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDVFVLVVAPDRCTDCRKCVNACERTHGHARIYKISEDAPPVTCLQCDDAPCANVCPVN
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCHH
AIVEEGDSWIVTEDCVGCGLCAVACPFGAIEMVNGRADKCTLCVDAPKKVPACVEACDRG
HHHHCCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHCCC
ALKLVSKSQVAEEKRERFAIEMERIYRTLAKLESEEGSLRGESGSRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDVFVLVVAPDRCTDCRKCVNACERTHGHARIYKISEDAPPVTCLQCDDAPCANVCPVN
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCHH
AIVEEGDSWIVTEDCVGCGLCAVACPFGAIEMVNGRADKCTLCVDAPKKVPACVEACDRG
HHHHCCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHCCC
ALKLVSKSQVAEEKRERFAIEMERIYRTLAKLESEEGSLRGESGSRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]