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The map label for this gene is truD

Identifier: 20094114

GI number: 20094114

Start: 633524

End: 634726

Strand: Direct

Name: truD

Synonym: MK0676

Alternate gene names: 20094114

Gene position: 633524-634726 (Clockwise)

Preceding gene: 20094113

Following gene: 20094116

Centisome position: 37.38

GC content: 63.67

Gene sequence:

>1203_bases
ATGGAAAAGCCCTCGAGGAACACCTACATTCCCCTGACCATCGTCGGTGAGTGTCCCGAGTGCCGCGAGTACCACCGAGG
GCGGGAGTGTCCCGAGTGCGGGCAGGATCTACGCCGACCGCGGCTTCGACCTCAGTTCGGGGGTCGCGGTCCACATACGC
TGTTCTACCTGGAGAAGTACGACTGGGACACCATGAAGGCCGTACGTAGGATCGCACAGGCCTTGAGGAAGCACCACCGT
CACTTCGGAATCGCCGGCATGAAGGACAAGCGGGCCGTCACCTCCCAGCGGGTAACCGTGCGGGGGGTACCTCCCGGAGT
CCTGGCCCGGCTCAGGATCCGCGACCTCAAGATCGTACCGATGGGCCGTGCTCGACGCAAGCTACGTCCCGGAGACCTGT
GGGGAAACAGGTTCGTTATCACCGTGCGTGGCGCGAAGGTACGTCGACTCCCCGAAGCCCTGAGGACGGTTCGGGAGCTC
GGAGGCGTGCCCAACTACTACGGTCTCCAGCGGTTCGGCAGCCGACGCCCCGTCACCCACGTCGTCGGAAAGTACGTGGT
ACTCGAGGACTGGGAGAAAGCCGTCAAGACCTTCCTGACCCTCGAATACCCCCGCGAGTCACCTGAAGCCCTCGAGGCTC
GACGGTGGTTGAAGGAACACTGGGGGGAGTTCAAGGAAGCACTACGGAGGTTCCCCAAGTTCCTCGACTACGAGCGTCAC
ATCCTCGAGCACTTGGCCCGACATCCGCATGACTATATCAACGCCTTCCGTCGCCTACCGATGTGGATACGTAGAATGTT
CGTGCACGCTTACCAGTCGTACCTGTTCAACCGCATCCTCTCGGAGAGGATCGCCCGGGGTCTCCCGGTCCACCGGCCCG
TCGAGGGGGACGTGACCCAGGACGGCCTGCCCACCGTCCCCCTCCCCGGGTTCCGCACGGAGCTCAGCGACGGCCCACAG
GGCGAAATAGAGCGTGAGGTGTTGGAGGAGGAGGGCGTGAGGCTGGAGGATTTCGAAATAAAGAGCATGCCGGAGCTCTC
GGCCGGCGGAGACCGGAAGCCGGCGTTGCTCCGAGTGTACGGGCTGCGTGCCGAGGCGATCGGTGACGATACCGTACGGT
TCACGTTCTCGCTGCCTCGTGGCGGTTACGCGACCACCGTCCTGCGGGAGCTCTTAGGGTCGGAGGGAGTGTACGCCGAC
TAG

Upstream 100 bases:

>100_bases
GACGCTGAGGGACGTGGAGGAGTACGTCGGAATACGGGAGCGGGTGATCGATCGAGGTGTCGGAGGGTCGTGCAGGGACA
GGGTGGAGGACTTCCTGGTC

Downstream 100 bases:

>100_bases
TACTCGTAGAAGGCGGCGCTACCGCACTCCTCGCACAGCCCCGCGAACCGCAGCTTTCCACGCTCTCGCTTGGCCCTCTT
GTACTCCTCCTTCGTGCAGT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MEKPSRNTYIPLTIVGECPECREYHRGRECPECGQDLRRPRLRPQFGGRGPHTLFYLEKYDWDTMKAVRRIAQALRKHHR
HFGIAGMKDKRAVTSQRVTVRGVPPGVLARLRIRDLKIVPMGRARRKLRPGDLWGNRFVITVRGAKVRRLPEALRTVREL
GGVPNYYGLQRFGSRRPVTHVVGKYVVLEDWEKAVKTFLTLEYPRESPEALEARRWLKEHWGEFKEALRRFPKFLDYERH
ILEHLARHPHDYINAFRRLPMWIRRMFVHAYQSYLFNRILSERIARGLPVHRPVEGDVTQDGLPTVPLPGFRTELSDGPQ
GEIEREVLEEEGVRLEDFEIKSMPELSAGGDRKPALLRVYGLRAEAIGDDTVRFTFSLPRGGYATTVLRELLGSEGVYAD

Sequences:

>Translated_400_residues
MEKPSRNTYIPLTIVGECPECREYHRGRECPECGQDLRRPRLRPQFGGRGPHTLFYLEKYDWDTMKAVRRIAQALRKHHR
HFGIAGMKDKRAVTSQRVTVRGVPPGVLARLRIRDLKIVPMGRARRKLRPGDLWGNRFVITVRGAKVRRLPEALRTVREL
GGVPNYYGLQRFGSRRPVTHVVGKYVVLEDWEKAVKTFLTLEYPRESPEALEARRWLKEHWGEFKEALRRFPKFLDYERH
ILEHLARHPHDYINAFRRLPMWIRRMFVHAYQSYLFNRILSERIARGLPVHRPVEGDVTQDGLPTVPLPGFRTELSDGPQ
GEIEREVLEEEGVRLEDFEIKSMPELSAGGDRKPALLRVYGLRAEAIGDDTVRFTFSLPRGGYATTVLRELLGSEGVYAD
>Mature_400_residues
MEKPSRNTYIPLTIVGECPECREYHRGRECPECGQDLRRPRLRPQFGGRGPHTLFYLEKYDWDTMKAVRRIAQALRKHHR
HFGIAGMKDKRAVTSQRVTVRGVPPGVLARLRIRDLKIVPMGRARRKLRPGDLWGNRFVITVRGAKVRRLPEALRTVREL
GGVPNYYGLQRFGSRRPVTHVVGKYVVLEDWEKAVKTFLTLEYPRESPEALEARRWLKEHWGEFKEALRRFPKFLDYERH
ILEHLARHPHDYINAFRRLPMWIRRMFVHAYQSYLFNRILSERIARGLPVHRPVEGDVTQDGLPTVPLPGFRTELSDGPQ
GEIEREVLEEEGVRLEDFEIKSMPELSAGGDRKPALLRVYGLRAEAIGDDTVRFTFSLPRGGYATTVLRELLGSEGVYAD

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI50727002, Length=411, Percent_Identity=26.7639902676399, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI148762985, Length=422, Percent_Identity=25.3554502369668, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI148762961, Length=422, Percent_Identity=25.3554502369668, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI148762948, Length=422, Percent_Identity=25.3554502369668, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789102, Length=128, Percent_Identity=35.15625, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17557232, Length=389, Percent_Identity=28.5347043701799, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324817, Length=277, Percent_Identity=33.9350180505415, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355799, Length=245, Percent_Identity=34.6938775510204, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_METKA (Q8TXJ7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613961.1
- ProteinModelPortal:   Q8TXJ7
- SMR:   Q8TXJ7
- GeneID:   1476777
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0676
- NMPDR:   fig|190192.1.peg.674
- HOGENOM:   HBG541221
- OMA:   AYIGYQR
- BioCyc:   MKAN190192:MK0676-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 46488; Mature: 46488

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 89-89

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPSRNTYIPLTIVGECPECREYHRGRECPECGQDLRRPRLRPQFGGRGPHTLFYLEKY
CCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
DWDTMKAVRRIAQALRKHHRHFGIAGMKDKRAVTSQRVTVRGVPPGVLARLRIRDLKIVP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEECCCHHHHHHHHHHCEEEEC
MGRARRKLRPGDLWGNRFVITVRGAKVRRLPEALRTVRELGGVPNYYGLQRFGSRRPVTH
CCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
VVGKYVVLEDWEKAVKTFLTLEYPRESPEALEARRWLKEHWGEFKEALRRFPKFLDYERH
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEHLARHPHDYINAFRRLPMWIRRMFVHAYQSYLFNRILSERIARGLPVHRPVEGDVTQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DGLPTVPLPGFRTELSDGPQGEIEREVLEEEGVRLEDFEIKSMPELSAGGDRKPALLRVY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEE
GLRAEAIGDDTVRFTFSLPRGGYATTVLRELLGSEGVYAD
CCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEKPSRNTYIPLTIVGECPECREYHRGRECPECGQDLRRPRLRPQFGGRGPHTLFYLEKY
CCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
DWDTMKAVRRIAQALRKHHRHFGIAGMKDKRAVTSQRVTVRGVPPGVLARLRIRDLKIVP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEECCCHHHHHHHHHHCEEEEC
MGRARRKLRPGDLWGNRFVITVRGAKVRRLPEALRTVRELGGVPNYYGLQRFGSRRPVTH
CCHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
VVGKYVVLEDWEKAVKTFLTLEYPRESPEALEARRWLKEHWGEFKEALRRFPKFLDYERH
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILEHLARHPHDYINAFRRLPMWIRRMFVHAYQSYLFNRILSERIARGLPVHRPVEGDVTQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DGLPTVPLPGFRTELSDGPQGEIEREVLEEEGVRLEDFEIKSMPELSAGGDRKPALLRVY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEE
GLRAEAIGDDTVRFTFSLPRGGYATTVLRELLGSEGVYAD
CCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014