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The map label for this gene is PRI1 [H]

Identifier: 20094024

GI number: 20094024

Start: 545004

End: 545954

Strand: Direct

Name: PRI1 [H]

Synonym: MK0586

Alternate gene names: 20094024

Gene position: 545004-545954 (Clockwise)

Preceding gene: 20094023

Following gene: 20094025

Centisome position: 32.15

GC content: 59.1

Gene sequence:

>951_bases
TTGGACTTCTATTCGCCAACCTTCCACAGCCGAGAGTGTGAAACTGCGGGCGCGAGGAAGATGGACGTGAAGACGTTAGT
CGGAGAGTACTACGCCTCACTTCCCAGCGGCGTGTTACGAGAGTTCGTGGAAGACCCGAGCTCCCGCGAGTGGGCGTACA
CCTTCCTAAAGGGACGGGATATCGTATTCACCCGAAAGCGGAGGTTGGACGAGGTCTCAGGGTTCTTAGAGCTGTATCAC
TCGACTGGGAAGTTCAAGAACCCGAAATCGTGGGAGGGACTCCTCGGCTGGGATCTGGTGATCGACGTGGACGCGGAGCT
TCCGGAGGAGCCCGAGGCGTTCCTCAAATCGCTGGGGAGGCTACTCAAGGATGTAGTGATAGCATGCGACGAGTTACGTC
GGGCGTTAGGGTTCCCACGACCGGACGTGGTTAATTTCTCTGGATCCAAGGGGTTTCACGTGAGGTACTTCGACAGCACC
GTGCGACGGTGGCTGAGATGGGACTTACACGAGCGTCGAGGTATAAAGCCGGGCGAGATCATCCAGCGGGTGGGTCGTGG
AGTCGTATGGTTGGCACGTGAGGGTTTCGTGGCTGGGGACAGAGTGCGTGCACTCCGCGAGGGGCTCTTGGATGACTCGA
TGTACGATCTCAAGAGACTGATCCGATGCGTCGGATCGATGAACGTCAAGTCGCTACTCCCCGCAGTGCCGGTGTGGTCG
CGAGAGGGGGGTCGGTGGGTGGACTTTCGGGACGAGGTCCTAGGAATGAACGATTTGGAGCTGGGATGCCTGGTCGCCCA
CCGCACGTTGACCTGGCCCGGCCGTGGGGGATTGGGCGTAGTACTTTCCCGCGTACTCGACTTAGACACGGATGCAGATC
CGGAGGATCCAAGCGACTTCGTCGAGGTATGGGGGAACGTGATGTCGGTGCTGGGGGAGCTTAAGCCGTGA

Upstream 100 bases:

>100_bases
ATCGAGCTAATTCCTCTACTTTCATTGAGTGAACGAGGGTAATAGTAGACGGTTATGGCGCGCGATGGCAGATAAGTAAC
CAAATAACGTCCTTTTTTAT

Downstream 100 bases:

>100_bases
GGTTCGTGGTGCCGTTCGCGGATAGGGGGGATCGTAAGACCCGGCTGTCCTCGTGCATGGACGAGGAGACGCGTGAGCGG
TTCGCGTTGGCGATGCTAAG

Product: DNA primase, small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MDFYSPTFHSRECETAGARKMDVKTLVGEYYASLPSGVLREFVEDPSSREWAYTFLKGRDIVFTRKRRLDEVSGFLELYH
STGKFKNPKSWEGLLGWDLVIDVDAELPEEPEAFLKSLGRLLKDVVIACDELRRALGFPRPDVVNFSGSKGFHVRYFDST
VRRWLRWDLHERRGIKPGEIIQRVGRGVVWLAREGFVAGDRVRALREGLLDDSMYDLKRLIRCVGSMNVKSLLPAVPVWS
REGGRWVDFRDEVLGMNDLELGCLVAHRTLTWPGRGGLGVVLSRVLDLDTDADPEDPSDFVEVWGNVMSVLGELKP

Sequences:

>Translated_316_residues
MDFYSPTFHSRECETAGARKMDVKTLVGEYYASLPSGVLREFVEDPSSREWAYTFLKGRDIVFTRKRRLDEVSGFLELYH
STGKFKNPKSWEGLLGWDLVIDVDAELPEEPEAFLKSLGRLLKDVVIACDELRRALGFPRPDVVNFSGSKGFHVRYFDST
VRRWLRWDLHERRGIKPGEIIQRVGRGVVWLAREGFVAGDRVRALREGLLDDSMYDLKRLIRCVGSMNVKSLLPAVPVWS
REGGRWVDFRDEVLGMNDLELGCLVAHRTLTWPGRGGLGVVLSRVLDLDTDADPEDPSDFVEVWGNVMSVLGELKP
>Mature_316_residues
MDFYSPTFHSRECETAGARKMDVKTLVGEYYASLPSGVLREFVEDPSSREWAYTFLKGRDIVFTRKRRLDEVSGFLELYH
STGKFKNPKSWEGLLGWDLVIDVDAELPEEPEAFLKSLGRLLKDVVIACDELRRALGFPRPDVVNFSGSKGFHVRYFDST
VRRWLRWDLHERRGIKPGEIIQRVGRGVVWLAREGFVAGDRVRALREGLLDDSMYDLKRLIRCVGSMNVKSLLPAVPVWS
REGGRWVDFRDEVLGMNDLELGCLVAHRTLTWPGRGGLGVVLSRVLDLDTDADPEDPSDFVEVWGNVMSVLGELKP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.-

Molecular weight: Translated: 35978; Mature: 35978

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFYSPTFHSRECETAGARKMDVKTLVGEYYASLPSGVLREFVEDPSSREWAYTFLKGRD
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEHHHCCCC
IVFTRKRRLDEVSGFLELYHSTGKFKNPKSWEGLLGWDLVIDVDAELPEEPEAFLKSLGR
EEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LLKDVVIACDELRRALGFPRPDVVNFSGSKGFHVRYFDSTVRRWLRWDLHERRGIKPGEI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHH
IQRVGRGVVWLAREGFVAGDRVRALREGLLDDSMYDLKRLIRCVGSMNVKSLLPAVPVWS
HHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCC
REGGRWVDFRDEVLGMNDLELGCLVAHRTLTWPGRGGLGVVLSRVLDLDTDADPEDPSDF
CCCCCEECCHHHHCCCCCCHHHHHHHHCEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
VEVWGNVMSVLGELKP
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFYSPTFHSRECETAGARKMDVKTLVGEYYASLPSGVLREFVEDPSSREWAYTFLKGRD
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEHHHCCCC
IVFTRKRRLDEVSGFLELYHSTGKFKNPKSWEGLLGWDLVIDVDAELPEEPEAFLKSLGR
EEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LLKDVVIACDELRRALGFPRPDVVNFSGSKGFHVRYFDSTVRRWLRWDLHERRGIKPGEI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHH
IQRVGRGVVWLAREGFVAGDRVRALREGLLDDSMYDLKRLIRCVGSMNVKSLLPAVPVWS
HHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCC
REGGRWVDFRDEVLGMNDLELGCLVAHRTLTWPGRGGLGVVLSRVLDLDTDADPEDPSDF
CCCCCEECCHHHHCCCCCCHHHHHHHHCEECCCCCCCHHHHHHHHHHCCCCCCCCCHHHH
VEVWGNVMSVLGELKP
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]