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The map label for this gene is Exo

Identifier: 20094004

GI number: 20094004

Start: 528389

End: 529435

Strand: Direct

Name: Exo

Synonym: MK0566

Alternate gene names: 20094004

Gene position: 528389-529435 (Clockwise)

Preceding gene: 20094003

Following gene: 20094006

Centisome position: 31.17

GC content: 59.6

Gene sequence:

>1047_bases
TTGGGACTAGCTGAACTCCGAGAACTGATCGAACCCGAAGAGACGGACCTGAGAGCCCTCGCCGGTCGGGAGATCGCTAT
CGACGCGTTCAACGCCCTGTATCAATTCCTGACCACGATCATGAAGGACGGACGACCTCTCATGGACTCGAGGGGCAGGA
TTACCAGCCACTTAAATGGCCTCCTGTATAGGACCGTGAACTTGGTCGAAGAGGGTATCAAGCCGGTATACGTGTTCGAT
GGTGAGCCCCCGGACCTGAAGCGTGAAACGCTGGAGCGTCGACGGGAACGGAAGGAGGAGGCGATGGAGAAACTGAGGCG
GGCCAAAACGAAGGAGGAGCGGGAGAAGTACGCCCGACAAGTCGCCAGACTCGACGAGTCGTTGGTGGAAGACGCGAAGA
GGCTGTTGGATCTCATGGGCATCCCGTGGGTACAGGCCCCCTCGGAAGGAGAGGCGCAGTGCGCGTATATGGCGAGGTGC
GGGGACGTATGGGCGACAGGCAGCCAAGACTACGACTCGCTGCTTTTCGGCAGCCCCAGGTTGGTTCGCAACATCACGAT
AGTCGGAAAGCGGAAGCATCCACACACCGGCGAGATCATAGAGGTCAAGCCCGAGATCATGAGGTTGGAGGACGTGCTCG
ACCAGCTGGGATTGGAATCGAGGGAGCAGCTGGTGGACCTAGCGATCCTTTTGGGCACGGACTACAACCCGGATGGAGTA
CCCGGGATTGGTCCGAAGCGCGCGCTGCAGTTGATCAGGAAGTACGGGTCGCTAGACGAGCTTAAGGACACCGACATCTG
GCCTAAGATCGAGCGGCACCTGCCGGTGGAACCGGAGAAGCTCAGAAGGCTCTTCCTCGAGCCGGAAGTTACGGACGACT
ACGAGCTAGACTGGGACGAACCCGACGAAGAGGGACTGGTCGAGTTCCTGGTTGAGGAGCGTGATTTCTCCGAGGATCGA
GTCCGCCGCGCCGTCGAGCGTCTGAAGGAGGCACTTCAGGAGTTGCGGAAGGGCGGTCGCCAAGAGACCCTGGACGCGTT
CTTCTGA

Upstream 100 bases:

>100_bases
TTCGATGGCGGTGCGAAACGCCTTCGACTTTGAGTAGGGGACAATAGCGCCACCCGGGTAGTTATAAGTTCGATCCACAG
GGGTTCCTGGAGGGGACAAT

Downstream 100 bases:

>100_bases
CCCGAGATTCCAGGCGGCGTCGATGGAGCTCAGGGGTGGATCTCCGAACGCTCAGCTCGAGGTTTGTTCAGAAACCCCCG
AGCACACCGAGTGGAGGCAC

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MGLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNGLLYRTVNLVEEGIKPVYVFD
GEPPDLKRETLERRRERKEEAMEKLRRAKTKEEREKYARQVARLDESLVEDAKRLLDLMGIPWVQAPSEGEAQCAYMARC
GDVWATGSQDYDSLLFGSPRLVRNITIVGKRKHPHTGEIIEVKPEIMRLEDVLDQLGLESREQLVDLAILLGTDYNPDGV
PGIGPKRALQLIRKYGSLDELKDTDIWPKIERHLPVEPEKLRRLFLEPEVTDDYELDWDEPDEEGLVEFLVEERDFSEDR
VRRAVERLKEALQELRKGGRQETLDAFF

Sequences:

>Translated_348_residues
MGLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNGLLYRTVNLVEEGIKPVYVFD
GEPPDLKRETLERRRERKEEAMEKLRRAKTKEEREKYARQVARLDESLVEDAKRLLDLMGIPWVQAPSEGEAQCAYMARC
GDVWATGSQDYDSLLFGSPRLVRNITIVGKRKHPHTGEIIEVKPEIMRLEDVLDQLGLESREQLVDLAILLGTDYNPDGV
PGIGPKRALQLIRKYGSLDELKDTDIWPKIERHLPVEPEKLRRLFLEPEVTDDYELDWDEPDEEGLVEFLVEERDFSEDR
VRRAVERLKEALQELRKGGRQETLDAFF
>Mature_347_residues
GLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNGLLYRTVNLVEEGIKPVYVFDG
EPPDLKRETLERRRERKEEAMEKLRRAKTKEEREKYARQVARLDESLVEDAKRLLDLMGIPWVQAPSEGEAQCAYMARCG
DVWATGSQDYDSLLFGSPRLVRNITIVGKRKHPHTGEIIEVKPEIMRLEDVLDQLGLESREQLVDLAILLGTDYNPDGVP
GIGPKRALQLIRKYGSLDELKDTDIWPKIERHLPVEPEKLRRLFLEPEVTDDYELDWDEPDEEGLVEFLVEERDFSEDRV
RRAVERLKEALQELRKGGRQETLDAFF

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily

Homologues:

Organism=Homo sapiens, GI4758356, Length=358, Percent_Identity=42.4581005586592, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI194018535, Length=256, Percent_Identity=32.8125, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI194018531, Length=256, Percent_Identity=32.8125, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI51988900, Length=304, Percent_Identity=27.3026315789474, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI284055276, Length=343, Percent_Identity=25.9475218658892, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI284172361, Length=343, Percent_Identity=25.9475218658892, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI284055278, Length=343, Percent_Identity=25.9475218658892, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17510005, Length=364, Percent_Identity=40.3846153846154, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322736, Length=365, Percent_Identity=39.7260273972603, Blast_Score=246, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6321697, Length=197, Percent_Identity=30.4568527918782, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324607, Length=270, Percent_Identity=23.7037037037037, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320469, Length=203, Percent_Identity=26.1083743842365, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17647423, Length=356, Percent_Identity=41.5730337078652, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24658219, Length=275, Percent_Identity=28, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI78706850, Length=179, Percent_Identity=28.4916201117318, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78706852, Length=179, Percent_Identity=28.4916201117318, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78706854, Length=179, Percent_Identity=28.4916201117318, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FEN_METKA (Q8TXU4)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613851.1
- ProteinModelPortal:   Q8TXU4
- SMR:   Q8TXU4
- GeneID:   1476667
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0566
- NMPDR:   fig|190192.1.peg.564
- HOGENOM:   HBG316651
- OMA:   DYDSLLF
- ProtClustDB:   PRK03980
- BioCyc:   MKAN190192:MK0566-MONOMER
- HAMAP:   MF_00614
- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR019974
- InterPro:   IPR006085
- InterPro:   IPR006084
- PANTHER:   PTHR11081
- PRINTS:   PR00853
- SMART:   SM00279
- SMART:   SM00484
- SMART:   SM00485
- TIGRFAMs:   TIGR03674

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N; SSF47807 5_3_exo_C

EC number: 3.-.-.-

Molecular weight: Translated: 40252; Mature: 40121

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00841 XPG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNG
CCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LLYRTVNLVEEGIKPVYVFDGEPPDLKRETLERRRERKEEAMEKLRRAKTKEEREKYARQ
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
VARLDESLVEDAKRLLDLMGIPWVQAPSEGEAQCAYMARCGDVWATGSQDYDSLLFGSPR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCHHHHHCCCCH
LVRNITIVGKRKHPHTGEIIEVKPEIMRLEDVLDQLGLESREQLVDLAILLGTDYNPDGV
HHHEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
PGIGPKRALQLIRKYGSLDELKDTDIWPKIERHLPVEPEKLRRLFLEPEVTDDYELDWDE
CCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
PDEEGLVEFLVEERDFSEDRVRRAVERLKEALQELRKGGRQETLDAFF
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
GLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNG
CHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LLYRTVNLVEEGIKPVYVFDGEPPDLKRETLERRRERKEEAMEKLRRAKTKEEREKYARQ
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
VARLDESLVEDAKRLLDLMGIPWVQAPSEGEAQCAYMARCGDVWATGSQDYDSLLFGSPR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCHHHHHCCCCH
LVRNITIVGKRKHPHTGEIIEVKPEIMRLEDVLDQLGLESREQLVDLAILLGTDYNPDGV
HHHEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
PGIGPKRALQLIRKYGSLDELKDTDIWPKIERHLPVEPEKLRRLFLEPEVTDDYELDWDE
CCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCC
PDEEGLVEFLVEERDFSEDRVRRAVERLKEALQELRKGGRQETLDAFF
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014