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The map label for this gene is ThrS

Identifier: 20093982

GI number: 20093982

Start: 507396

End: 509270

Strand: Direct

Name: ThrS

Synonym: MK0544

Alternate gene names: 20093982

Gene position: 507396-509270 (Clockwise)

Preceding gene: 20093980

Following gene: 20093984

Centisome position: 29.94

GC content: 57.97

Gene sequence:

>1875_bases
GTGAGGTTACTGTTCATTCACGCGGACGAGATGTCGTTCGAGGCGAGGCAGAAGACCAAGATCGCCGAGGAAGAGCCGCC
TATTAAGGAAGCGGAAGTTGAGGACTGTCTGGTAGTCTTCGCGGCGGTCCAAGAAGCTGATGAAGAGAATCCAAAGGCCA
TCGCCGAGGCGGCCGTAGAGGAGATCGAAGACGTTGCGGGTGAATTGAAGGCCGACAGGATAGTCTTGTACCCGTACGCT
CATCTAGCCGACGATCTCGCTTCCCCGGACGTGGCCGTCGAGGTTCTGAAGAGAATGGAAGGGCTCCTGAAGGAGCGCGG
GTACGAGGTCGTGAGAGCCCCCTTCGGGTGGTACAAGGCATTCCGATTGGCGTGCAAGGGGCATCCACTCTCGGAGCTCT
CCAGGACGGTAACCCCCGAGGCCGCCGAGGAAGCTGAGGAGGAGAAGATCGAGTCCGAGTTCTTGGTGTACATGGACGGG
GAATTGATACCGGTAGAAGAAGTGGATCTCTCGGAACTTCCCGAGGACTTCAGGCACCTAGTGATGCACGAGTTGGGTGA
AGAGCGTGAAACGGGCGACGAAGAGCCCGCACACGTGAAGCTGATGCGGGAGAAGGAAATCTGCGACCACGAGCCGGCAG
CGGACGTTGGACACGTACGGTGGTACCCGAAGGGTCACGTGGTCAGGAGATGCCTGGCGGAGTACGTGGAGAACCTCATG
GCCGATCTAGGAGCGGCGGTCGTCGAGACACCCGTGATGTACGATCTGAGTGAGGACGCTATCCGGGAGCACGCCGACAA
GTTCGGGGAGCGACAGTATCGGATAAGGGCTGGAAACCGGGCACTGATGCTGAGGTACGCGGCCTGCTTCGGAGCCTTCA
GGCTACTAGCGGACACCACGCTCAGCCGGCGTCACCTACCCTTGAAGATCTACGAACTATCACAAAGCTTCAGGTTAGAG
CAGAGTGGTGAGGTGGTCGGACTGAAGCGGTTACGGGCGTTCACTATGCCCGATCTCCACACCGTTTGCGCCGACATGGA
CGAGGCCGTGGAAGAGTTCCTCGAGCAAGCGAAACTATGCTTGGAGGTAGGGCTGGACCTCGGGTTGGAATATGAGGTAG
TGTTCAGGACCACGGAGAAGTTCCTGAAGGAGAGGAAAGAAGTCCTAGAGGAACTGGCCGAGGCCATGGAAAAAGCGTAC
GGTGACGCGAAGCCCGTACTGGTGGAGGTACTGCCGGAACGTAAACACTACTGGGAATGTAAGGTGGACTTCGCGTTCAT
TGATAGCCTCGGAAGACCCATCGAGAACCCGACGGTACAGATCGACGTCGAGAGTGGGAGGCGCTTCGGAATCACGTACG
CGGACGAGTCGGGCGATGAACGTCATCCAGTGATATTACACTGCTCACCGACAGGGAGCCTTGAACGTGTAATATGCGCG
ATCCTCGAGGGCCAGTACAAGCGGTTCGAGCAGGAGGGTAAGCTGCCAACTCTACCGACGTGGCTGTCGCCGGTACAGGC
GCGTGTGATTCCGGTTTCAGAGAAAGTGCTGGAAGAAGCTGAGAAAGTCTTCGAAGAGCTGAAAAGTGAGGGGTTCCGAG
TCGACCTGGACGACCGCGATGAGCCGGTGGGACGTAAGATCCGAGATGCGGGTGAGGAGTGGGTACCGTACGTCATCGTG
ATTGGTGAGGAGGAAGTGAAGAAAGGCACGCTCTCGGTAACGATACGGGAGGAATCCACGTTGAAAGAACAGCGGCGTGA
AGAGATGACGTTAGAGGAGCTCGTAGAGCGGTTGGAGCGCGAAACGGAGGGCAAACCGAGGGTCCCACTAACGATACCGG
ATCGCCTCAGTCGGCGTCCGCGGTTCGGCAGGTAA

Upstream 100 bases:

>100_bases
GAGGTGTTGGGCACTTCCGGCAGTGAGTGAACACTTCTTCCCCCACGTGGGTCCGATGATGCTATCAGATTGTTCCCGAG
GTCCGACCGGGGCCGGCTAA

Downstream 100 bases:

>100_bases
CTCACGTCTCCCTCTCCATCGCCACAATACCCGTCCTAGCGATTTCGACGATGTTACCGATATCCTTCATCAACTCAAGG
AGGGATTCGACATCCTGCGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MRLLFIHADEMSFEARQKTKIAEEEPPIKEAEVEDCLVVFAAVQEADEENPKAIAEAAVEEIEDVAGELKADRIVLYPYA
HLADDLASPDVAVEVLKRMEGLLKERGYEVVRAPFGWYKAFRLACKGHPLSELSRTVTPEAAEEAEEEKIESEFLVYMDG
ELIPVEEVDLSELPEDFRHLVMHELGEERETGDEEPAHVKLMREKEICDHEPAADVGHVRWYPKGHVVRRCLAEYVENLM
ADLGAAVVETPVMYDLSEDAIREHADKFGERQYRIRAGNRALMLRYAACFGAFRLLADTTLSRRHLPLKIYELSQSFRLE
QSGEVVGLKRLRAFTMPDLHTVCADMDEAVEEFLEQAKLCLEVGLDLGLEYEVVFRTTEKFLKERKEVLEELAEAMEKAY
GDAKPVLVEVLPERKHYWECKVDFAFIDSLGRPIENPTVQIDVESGRRFGITYADESGDERHPVILHCSPTGSLERVICA
ILEGQYKRFEQEGKLPTLPTWLSPVQARVIPVSEKVLEEAEKVFEELKSEGFRVDLDDRDEPVGRKIRDAGEEWVPYVIV
IGEEEVKKGTLSVTIREESTLKEQRREEMTLEELVERLERETEGKPRVPLTIPDRLSRRPRFGR

Sequences:

>Translated_624_residues
MRLLFIHADEMSFEARQKTKIAEEEPPIKEAEVEDCLVVFAAVQEADEENPKAIAEAAVEEIEDVAGELKADRIVLYPYA
HLADDLASPDVAVEVLKRMEGLLKERGYEVVRAPFGWYKAFRLACKGHPLSELSRTVTPEAAEEAEEEKIESEFLVYMDG
ELIPVEEVDLSELPEDFRHLVMHELGEERETGDEEPAHVKLMREKEICDHEPAADVGHVRWYPKGHVVRRCLAEYVENLM
ADLGAAVVETPVMYDLSEDAIREHADKFGERQYRIRAGNRALMLRYAACFGAFRLLADTTLSRRHLPLKIYELSQSFRLE
QSGEVVGLKRLRAFTMPDLHTVCADMDEAVEEFLEQAKLCLEVGLDLGLEYEVVFRTTEKFLKERKEVLEELAEAMEKAY
GDAKPVLVEVLPERKHYWECKVDFAFIDSLGRPIENPTVQIDVESGRRFGITYADESGDERHPVILHCSPTGSLERVICA
ILEGQYKRFEQEGKLPTLPTWLSPVQARVIPVSEKVLEEAEKVFEELKSEGFRVDLDDRDEPVGRKIRDAGEEWVPYVIV
IGEEEVKKGTLSVTIREESTLKEQRREEMTLEELVERLERETEGKPRVPLTIPDRLSRRPRFGR
>Mature_624_residues
MRLLFIHADEMSFEARQKTKIAEEEPPIKEAEVEDCLVVFAAVQEADEENPKAIAEAAVEEIEDVAGELKADRIVLYPYA
HLADDLASPDVAVEVLKRMEGLLKERGYEVVRAPFGWYKAFRLACKGHPLSELSRTVTPEAAEEAEEEKIESEFLVYMDG
ELIPVEEVDLSELPEDFRHLVMHELGEERETGDEEPAHVKLMREKEICDHEPAADVGHVRWYPKGHVVRRCLAEYVENLM
ADLGAAVVETPVMYDLSEDAIREHADKFGERQYRIRAGNRALMLRYAACFGAFRLLADTTLSRRHLPLKIYELSQSFRLE
QSGEVVGLKRLRAFTMPDLHTVCADMDEAVEEFLEQAKLCLEVGLDLGLEYEVVFRTTEKFLKERKEVLEELAEAMEKAY
GDAKPVLVEVLPERKHYWECKVDFAFIDSLGRPIENPTVQIDVESGRRFGITYADESGDERHPVILHCSPTGSLERVICA
ILEGQYKRFEQEGKLPTLPTWLSPVQARVIPVSEKVLEEAEKVFEELKSEGFRVDLDDRDEPVGRKIRDAGEEWVPYVIV
IGEEEVKKGTLSVTIREESTLKEQRREEMTLEELVERLERETEGKPRVPLTIPDRLSRRPRFGR

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=428, Percent_Identity=28.2710280373832, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI38570111, Length=392, Percent_Identity=28.3163265306122, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI20070344, Length=411, Percent_Identity=25.7907542579075, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI1788013, Length=393, Percent_Identity=26.972010178117, Blast_Score=146, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71984184, Length=392, Percent_Identity=27.5510204081633, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71984192, Length=392, Percent_Identity=27.5510204081633, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6322654, Length=419, Percent_Identity=24.1050119331742, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6322112, Length=422, Percent_Identity=24.8815165876777, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583842, Length=420, Percent_Identity=26.4285714285714, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI28574141, Length=420, Percent_Identity=26.4285714285714, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24583839, Length=420, Percent_Identity=26.4285714285714, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_METKA (Q8TXW5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613829.1
- ProteinModelPortal:   Q8TXW5
- SMR:   Q8TXW5
- GeneID:   1476645
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0544
- NMPDR:   fig|190192.1.peg.542
- HOGENOM:   HBG392335
- OMA:   ETPVMYD
- ProtClustDB:   PRK03991
- BioCyc:   MKAN190192:MK0544-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- ProDom:   PD016189
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED; SSF52954 Anticodon_bd

EC number: =6.1.1.3

Molecular weight: Translated: 71463; Mature: 71463

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLFIHADEMSFEARQKTKIAEEEPPIKEAEVEDCLVVFAAVQEADEENPKAIAEAAVE
CEEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EIEDVAGELKADRIVLYPYAHLADDLASPDVAVEVLKRMEGLLKERGYEVVRAPFGWYKA
HHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
FRLACKGHPLSELSRTVTPEAAEEAEEEKIESEFLVYMDGELIPVEEVDLSELPEDFRHL
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCEECCCCCCHHHCCHHHHHH
VMHELGEERETGDEEPAHVKLMREKEICDHEPAADVGHVRWYPKGHVVRRCLAEYVENLM
HHHHHCCHHCCCCCCCHHEEHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
ADLGAAVVETPVMYDLSEDAIREHADKFGERQYRIRAGNRALMLRYAACFGAFRLLADTT
HHHHHHHHCCCCEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LSRRHLPLKIYELSQSFRLEQSGEVVGLKRLRAFTMPDLHTVCADMDEAVEEFLEQAKLC
HHCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LEVGLDLGLEYEVVFRTTEKFLKERKEVLEELAEAMEKAYGDAKPVLVEVLPERKHYWEC
HHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEE
KVDFAFIDSLGRPIENPTVQIDVESGRRFGITYADESGDERHPVILHCSPTGSLERVICA
EEHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHH
ILEGQYKRFEQEGKLPTLPTWLSPVQARVIPVSEKVLEEAEKVFEELKSEGFRVDLDDRD
HHHHHHHHHHHCCCCCCCCHHHCCHHHEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCC
EPVGRKIRDAGEEWVPYVIVIGEEEVKKGTLSVTIREESTLKEQRREEMTLEELVERLER
CCCHHHHHHCCCCCCCEEEEECCHHHCCCEEEEEEECHHHHHHHHHHHCCHHHHHHHHHH
ETEGKPRVPLTIPDRLSRRPRFGR
HCCCCCCCCEECCHHHCCCCCCCC
>Mature Secondary Structure
MRLLFIHADEMSFEARQKTKIAEEEPPIKEAEVEDCLVVFAAVQEADEENPKAIAEAAVE
CEEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EIEDVAGELKADRIVLYPYAHLADDLASPDVAVEVLKRMEGLLKERGYEVVRAPFGWYKA
HHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
FRLACKGHPLSELSRTVTPEAAEEAEEEKIESEFLVYMDGELIPVEEVDLSELPEDFRHL
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCEECCCCCCHHHCCHHHHHH
VMHELGEERETGDEEPAHVKLMREKEICDHEPAADVGHVRWYPKGHVVRRCLAEYVENLM
HHHHHCCHHCCCCCCCHHEEHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
ADLGAAVVETPVMYDLSEDAIREHADKFGERQYRIRAGNRALMLRYAACFGAFRLLADTT
HHHHHHHHCCCCEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LSRRHLPLKIYELSQSFRLEQSGEVVGLKRLRAFTMPDLHTVCADMDEAVEEFLEQAKLC
HHCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LEVGLDLGLEYEVVFRTTEKFLKERKEVLEELAEAMEKAYGDAKPVLVEVLPERKHYWEC
HHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEE
KVDFAFIDSLGRPIENPTVQIDVESGRRFGITYADESGDERHPVILHCSPTGSLERVICA
EEHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHH
ILEGQYKRFEQEGKLPTLPTWLSPVQARVIPVSEKVLEEAEKVFEELKSEGFRVDLDDRD
HHHHHHHHHHHCCCCCCCCHHHCCHHHEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCC
EPVGRKIRDAGEEWVPYVIVIGEEEVKKGTLSVTIREESTLKEQRREEMTLEELVERLER
CCCHHHHHHCCCCCCCEEEEECCHHHCCCEEEEEEECHHHHHHHHHHHCCHHHHHHHHHH
ETEGKPRVPLTIPDRLSRRPRFGR
HCCCCCCCCEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014