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The map label for this gene is 20093972

Identifier: 20093972

GI number: 20093972

Start: 498533

End: 499327

Strand: Direct

Name: 20093972

Synonym: MK0534

Alternate gene names: NA

Gene position: 498533-499327 (Clockwise)

Preceding gene: 20093971

Following gene: 20093974

Centisome position: 29.41

GC content: 60.13

Gene sequence:

>795_bases
TTGAGAACCAACGTTTACCCAAGGGGCTGTGAGGATCGATTGCGCTTCGCCGCACTCTACTCCCACCAGTTCGGTAAGAG
GATGGTAGCCAACCTACTGAACGAACCCGGTCACTGCAGGGCTTGCGGGCCTGTATGCGACGGGTGCAAGTACGACATGT
ATTCGCTCGTCGACTCCCTCGTCGCCGTTGAGGAGCTACCCAAACCCGAAGAGTTACCTCCGTTCGTCGACGAGCCCGAG
GATCACCTCCCCGAACTACCGCCCGTCGATGTGCTGGTGGCGATCGGATTGCATCCCGACCTCCTAGTAGCGCTGTCCGA
AGTATACGAGATTAAGGCACTGATAGTGCCCGTCGAGGAACCCGACTGGATCGACCCGTGGATCGAAGAGAAACTACGAG
AGGTATGCGAAGAGAACAGCGTAGAGCTCACTGTGGCGAGGCCAGGATGCGACCTGGAACCGTCGGGTCCCGTAACCGAA
GCGTTCTGCGACGCGGGCATGATCGGGAGGCCTAAGCTGTTCATGAAGGTCGAGGACGGCGTCGTGATCGACGTCCATGT
AGTACGATCGGCGCCGTGCGGTTGCACGTGGTTCGTGGCTAAACGACTGGTCGGTGTGGACGCTGATCCCGAAGAGGTGA
AGGCTACAGTTTCCGAGGCACATCACTCGTACCCCTGCACGGCTTCGATGGAGGTCGACAAGCACGTGGGGGATACCCTG
CTCCACGTAGCGGGCCGGTTGCACATCGAGGCGGCCCTCCGAGCGCTCGAGGAAGCGACGGATACCGACGCCTGA

Upstream 100 bases:

>100_bases
GACCCCATCTTGAAGACGAGTCCTACAGCGATTCCCAAGGATCGCTTCCTCATCGCGAGATCACTCGAGACCTGAGGACA
AACCCTCATTCTCCCCGCCC

Downstream 100 bases:

>100_bases
CGGGTATCTCACGCCCCGCCCAGTACTCGCTCCAAAACTTCCCGGAACAGTCTCCACTTATCCCCGTGCAGACTGTACTC
CCTGCGCACGTCGTCCTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRTNVYPRGCEDRLRFAALYSHQFGKRMVANLLNEPGHCRACGPVCDGCKYDMYSLVDSLVAVEELPKPEELPPFVDEPE
DHLPELPPVDVLVAIGLHPDLLVALSEVYEIKALIVPVEEPDWIDPWIEEKLREVCEENSVELTVARPGCDLEPSGPVTE
AFCDAGMIGRPKLFMKVEDGVVIDVHVVRSAPCGCTWFVAKRLVGVDADPEEVKATVSEAHHSYPCTASMEVDKHVGDTL
LHVAGRLHIEAALRALEEATDTDA

Sequences:

>Translated_264_residues
MRTNVYPRGCEDRLRFAALYSHQFGKRMVANLLNEPGHCRACGPVCDGCKYDMYSLVDSLVAVEELPKPEELPPFVDEPE
DHLPELPPVDVLVAIGLHPDLLVALSEVYEIKALIVPVEEPDWIDPWIEEKLREVCEENSVELTVARPGCDLEPSGPVTE
AFCDAGMIGRPKLFMKVEDGVVIDVHVVRSAPCGCTWFVAKRLVGVDADPEEVKATVSEAHHSYPCTASMEVDKHVGDTL
LHVAGRLHIEAALRALEEATDTDA
>Mature_264_residues
MRTNVYPRGCEDRLRFAALYSHQFGKRMVANLLNEPGHCRACGPVCDGCKYDMYSLVDSLVAVEELPKPEELPPFVDEPE
DHLPELPPVDVLVAIGLHPDLLVALSEVYEIKALIVPVEEPDWIDPWIEEKLREVCEENSVELTVARPGCDLEPSGPVTE
AFCDAGMIGRPKLFMKVEDGVVIDVHVVRSAPCGCTWFVAKRLVGVDADPEEVKATVSEAHHSYPCTASMEVDKHVGDTL
LHVAGRLHIEAALRALEEATDTDA

Specific function: Is able to catalyze the biosynthesis of dTMP using dUMP, tetrahydrofolate and formaldehyde in vitro, i.e. a reaction equivalent to that catalyzed by bacterial thymidylate synthases (EC 2.1.1.45). However, M.jannaschii like most methanogenic Archaea lacks

COG id: COG1810

COG function: function code S; Uncharacterized protein conserved in archaea

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003745 [H]

Pfam domain/function: PF02593 DUF166 [H]

EC number: NA

Molecular weight: Translated: 29102; Mature: 29102

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTNVYPRGCEDRLRFAALYSHQFGKRMVANLLNEPGHCRACGPVCDGCKYDMYSLVDSL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHH
VAVEELPKPEELPPFVDEPEDHLPELPPVDVLVAIGLHPDLLVALSEVYEIKALIVPVEE
HHHHHCCCCCCCCCCCCCCHHHCCCCCCHHEEEEECCCHHHHHHHHHHHHEEEEEEECCC
PDWIDPWIEEKLREVCEENSVELTVARPGCDLEPSGPVTEAFCDAGMIGRPKLFMKVEDG
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCC
VVIDVHVVRSAPCGCTWFVAKRLVGVDADPEEVKATVSEAHHSYPCTASMEVDKHVGDTL
EEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LHVAGRLHIEAALRALEEATDTDA
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTNVYPRGCEDRLRFAALYSHQFGKRMVANLLNEPGHCRACGPVCDGCKYDMYSLVDSL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHH
VAVEELPKPEELPPFVDEPEDHLPELPPVDVLVAIGLHPDLLVALSEVYEIKALIVPVEE
HHHHHCCCCCCCCCCCCCCHHHCCCCCCHHEEEEECCCHHHHHHHHHHHHEEEEEEECCC
PDWIDPWIEEKLREVCEENSVELTVARPGCDLEPSGPVTEAFCDAGMIGRPKLFMKVEDG
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCC
VVIDVHVVRSAPCGCTWFVAKRLVGVDADPEEVKATVSEAHHSYPCTASMEVDKHVGDTL
EEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LHVAGRLHIEAALRALEEATDTDA
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]