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The map label for this gene is pncA [H]

Identifier: 20093942

GI number: 20093942

Start: 466093

End: 466626

Strand: Direct

Name: pncA [H]

Synonym: MK0504

Alternate gene names: 20093942

Gene position: 466093-466626 (Clockwise)

Preceding gene: 20093941

Following gene: 20093943

Centisome position: 27.5

GC content: 59.74

Gene sequence:

>534_bases
TTGCGCGCACTACTGATCGTCGATATGATCCGCGACTTCGTAGAGGAGGGAGCCCCGCTGGAGGTCCCGAAGGCACGTCG
ACTCGTACCCCGTATCGCCCGTCTCGCGGATGAGTTCAGGGAGCGAGGTGATCTGGTCGTTCACGTGTGGGACGAGCACT
ATCCGGACGATCCGGAGTTCAAGGTGTGGGGCGAACACGCCGTTGCGGGGACGGAGGGTGCGGAACCGGTAGAAGAACTG
AAACCGGAGGACGGCGACTTAGTGGTGCGGAAGCGGAAGTATAGCGGGTTCTACGGCACGTCTTTGGACTACGACCTGCG
CTCCCGGAACGTGAAAGAAATCTACCTCACGGGAGTGTGCACAGATATCTGTGTACTCTTCACATGCGCGGACGCGCTCA
TGCGAGGGTACCGAGTGTACGTAGTGAGGGACTGCGTGGCGTCGCTTGAGGAGGAATCCCACCGGTTCGCTTTGAAGCAC
ATGGAAAAGCTCGGGGCGGAGGTAGTCGATTCGGAGGAGATACTGGGGGACTGA

Upstream 100 bases:

>100_bases
CCGAGGAGTCCGCAGGTGGAGGCTGGCCTGCGCCGATGTGGTGGGCGCCCGCGCTTCTGGGAGTCGCGGCCGCACTGCTC
CGGTCGCGGGGGAGATGAAA

Downstream 100 bases:

>100_bases
CCGCCGTGAAGAGGATACTGGCGTTGGTGGACGGTGAGCACTACATCCCCGTAACTCGGGAGGCGTTAGAGACTGTGGAG
GAATTGGACCTCGGAGAGCT

Product: nicotinamidase-like amidase

Products: nicotinate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MRALLIVDMIRDFVEEGAPLEVPKARRLVPRIARLADEFRERGDLVVHVWDEHYPDDPEFKVWGEHAVAGTEGAEPVEEL
KPEDGDLVVRKRKYSGFYGTSLDYDLRSRNVKEIYLTGVCTDICVLFTCADALMRGYRVYVVRDCVASLEEESHRFALKH
MEKLGAEVVDSEEILGD

Sequences:

>Translated_177_residues
MRALLIVDMIRDFVEEGAPLEVPKARRLVPRIARLADEFRERGDLVVHVWDEHYPDDPEFKVWGEHAVAGTEGAEPVEEL
KPEDGDLVVRKRKYSGFYGTSLDYDLRSRNVKEIYLTGVCTDICVLFTCADALMRGYRVYVVRDCVASLEEESHRFALKH
MEKLGAEVVDSEEILGD
>Mature_177_residues
MRALLIVDMIRDFVEEGAPLEVPKARRLVPRIARLADEFRERGDLVVHVWDEHYPDDPEFKVWGEHAVAGTEGAEPVEEL
KPEDGDLVVRKRKYSGFYGTSLDYDLRSRNVKEIYLTGVCTDICVLFTCADALMRGYRVYVVRDCVASLEEESHRFALKH
MEKLGAEVVDSEEILGD

Specific function: Unknown

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=199, Percent_Identity=28.643216080402, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI87081970, Length=211, Percent_Identity=30.8056872037915, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI87081992, Length=190, Percent_Identity=27.8947368421053, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1786811, Length=178, Percent_Identity=25.2808988764045, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.19

Molecular weight: Translated: 20231; Mature: 20231

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALLIVDMIRDFVEEGAPLEVPKARRLVPRIARLADEFRERGDLVVHVWDEHYPDDPEF
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCE
KVWGEHAVAGTEGAEPVEELKPEDGDLVVRKRKYSGFYGTSLDYDLRSRNVKEIYLTGVC
EEECCEEECCCCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCEEEEEHHHH
TDICVLFTCADALMRGYRVYVVRDCVASLEEESHRFALKHMEKLGAEVVDSEEILGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MRALLIVDMIRDFVEEGAPLEVPKARRLVPRIARLADEFRERGDLVVHVWDEHYPDDPEF
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCE
KVWGEHAVAGTEGAEPVEELKPEDGDLVVRKRKYSGFYGTSLDYDLRSRNVKEIYLTGVC
EEECCEEECCCCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHCCCEEEEEHHHH
TDICVLFTCADALMRGYRVYVVRDCVASLEEESHRFALKHMEKLGAEVVDSEEILGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]