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The map label for this gene is dmeF [H]

Identifier: 197120049

GI number: 197120049

Start: 4214223

End: 4215230

Strand: Direct

Name: dmeF [H]

Synonym: Gbem_3688

Alternate gene names: 197120049

Gene position: 4214223-4215230 (Clockwise)

Preceding gene: 197120048

Following gene: 197120050

Centisome position: 91.31

GC content: 61.21

Gene sequence:

>1008_bases
ATGCATGCCCACGCCGACACCCATCTGGACCAGAGCATAACGGGTCGCTTCAGGTACGCCATAATCCTTACCGCTATCAC
GCTCGTTGCCGAACTGGCGGGGGGGCTTTGGACCAACTCCCTGGCCCTGCTCTCCGACGCTGCACACGTCTTCCTCGACC
TCTTCGCGCTGCTTCTTTCCCTGGGCGCCATCAAGCTCGCCTCCTACCCGGTCAGCGAGACCCGCACCTTCGGCTGGCAT
CGCATGGAGGTTTTCGCCTCCTTCATAAACGGCAGCACCGTGTTTCTCATCGCCGGGATCATCAGCTACGAGGCGCTAGT
ACGCTTCATCCATCCCGAAGAGGTGAAGAGCCTGGAGATGCTAATCATCGCCTTCGTCGGCCTGGTGATGAACCTCATCG
CGGCGGGCGCCCTCCACTCGCACTCACACGACGACCTGAACGTGCACAGCGCCTTTCTGCACGTGATCGGCGACGCCGCC
GCCTCAGTCGGCGTGATCATCGGCGGCATCATCATGTACTTCACCAACTGGTACCTTCTCGATGCAGTCATCTCCATCGG
CATCGGCTTCATCATCTTCTGGGGTTCGTGGCGGGTGATGCGCGAGTCAGTGCATATCCTTTTGGAAGGGGTTCCCCGCG
GCGTCGAGGTGGAACAGGTTTCGGCCGCTATCCTGGAGGTCGAAGGGGTGGAAGAGGTCCATCACGTCAATATCTGGACC
ATCTGCTCCCACATCCTGGCGTTGTCCGGGCACATCGTGGTTCCTCCCACGTTCAAAGGCGAGCATGGCCCAATCCTCAG
GAGGATCGAGGAGCGGCTTTTCGAGCGTTTCCACATCTCCCACACCACGCTCCAGTTGGAAACCACCCGTTGCACCGACA
CGGAGGGGGCGAAGCAGTTCCGCCACCGCCCGCGCGCGGCGTCTTTGGCCCATGCCCATGCCCATCCGCACCACCACCCC
CATGGCACTTGCCAGGGGGGGCATCACCATCACGACCACACACCTTAA

Upstream 100 bases:

>100_bases
GAATACCAGGAGCGCCCGATCTACTCCTTGAAGGCTGCCATAGGTTTCTAACAACCGTCAGTCGGGAGGGGGCTTTTGCC
CCCTCCCGCCGGGGTACACC

Downstream 100 bases:

>100_bases
CCCCTCGGACGGTAGATAATGCTTGATTACAACCTGATCGTCGACGCAGCCCAGAGACTCAAAAAAAGGGTGCGCCGGAC
GGAACTGATCCACGCTCAAC

Product: cobalt/zinc/iron/cadmium/nickel efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MHAHADTHLDQSITGRFRYAIILTAITLVAELAGGLWTNSLALLSDAAHVFLDLFALLLSLGAIKLASYPVSETRTFGWH
RMEVFASFINGSTVFLIAGIISYEALVRFIHPEEVKSLEMLIIAFVGLVMNLIAAGALHSHSHDDLNVHSAFLHVIGDAA
ASVGVIIGGIIMYFTNWYLLDAVISIGIGFIIFWGSWRVMRESVHILLEGVPRGVEVEQVSAAILEVEGVEEVHHVNIWT
ICSHILALSGHIVVPPTFKGEHGPILRRIEERLFERFHISHTTLQLETTRCTDTEGAKQFRHRPRAASLAHAHAHPHHHP
HGTCQGGHHHHDHTP

Sequences:

>Translated_335_residues
MHAHADTHLDQSITGRFRYAIILTAITLVAELAGGLWTNSLALLSDAAHVFLDLFALLLSLGAIKLASYPVSETRTFGWH
RMEVFASFINGSTVFLIAGIISYEALVRFIHPEEVKSLEMLIIAFVGLVMNLIAAGALHSHSHDDLNVHSAFLHVIGDAA
ASVGVIIGGIIMYFTNWYLLDAVISIGIGFIIFWGSWRVMRESVHILLEGVPRGVEVEQVSAAILEVEGVEEVHHVNIWT
ICSHILALSGHIVVPPTFKGEHGPILRRIEERLFERFHISHTTLQLETTRCTDTEGAKQFRHRPRAASLAHAHAHPHHHP
HGTCQGGHHHHDHTP
>Mature_335_residues
MHAHADTHLDQSITGRFRYAIILTAITLVAELAGGLWTNSLALLSDAAHVFLDLFALLLSLGAIKLASYPVSETRTFGWH
RMEVFASFINGSTVFLIAGIISYEALVRFIHPEEVKSLEMLIIAFVGLVMNLIAAGALHSHSHDDLNVHSAFLHVIGDAA
ASVGVIIGGIIMYFTNWYLLDAVISIGIGFIIFWGSWRVMRESVHILLEGVPRGVEVEQVSAAILEVEGVEEVHHVNIWT
ICSHILALSGHIVVPPTFKGEHGPILRRIEERLFERFHISHTTLQLETTRCTDTEGAKQFRHRPRAASLAHAHAHPHHHP
HGTCQGGHHHHDHTP

Specific function: Involved in divalent cation and potassium homeostasis in the cell. Catalyzes the active efflux of zinc, cadmium and cobalt, in exchange for potassium and H(+) ions [H]

COG id: COG1230

COG function: function code P; Co/Zn/Cd efflux system component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily [H]

Homologues:

Organism=Homo sapiens, GI52352805, Length=306, Percent_Identity=30.718954248366, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI21359890, Length=307, Percent_Identity=29.9674267100977, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI21361112, Length=294, Percent_Identity=30.952380952381, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI289803013, Length=290, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI289803009, Length=290, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI289803007, Length=290, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI289803003, Length=290, Percent_Identity=29.3103448275862, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI64762489, Length=274, Percent_Identity=29.9270072992701, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI14210520, Length=219, Percent_Identity=28.7671232876712, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI20070323, Length=287, Percent_Identity=25.7839721254355, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI222080086, Length=314, Percent_Identity=25.1592356687898, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI40255009, Length=314, Percent_Identity=25.1592356687898, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI52351208, Length=210, Percent_Identity=27.6190476190476, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786966, Length=278, Percent_Identity=34.8920863309352, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI193210990, Length=288, Percent_Identity=27.7777777777778, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17555794, Length=310, Percent_Identity=29.6774193548387, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17555796, Length=285, Percent_Identity=30.5263157894737, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564488, Length=298, Percent_Identity=28.1879194630872, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17550442, Length=126, Percent_Identity=32.5396825396825, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17550440, Length=126, Percent_Identity=32.5396825396825, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324892, Length=167, Percent_Identity=30.5389221556886, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323899, Length=145, Percent_Identity=35.1724137931034, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320411, Length=181, Percent_Identity=32.5966850828729, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583858, Length=322, Percent_Identity=28.8819875776398, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656728, Length=349, Percent_Identity=28.9398280802292, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24656724, Length=349, Percent_Identity=28.9398280802292, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24656720, Length=349, Percent_Identity=28.9398280802292, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24656715, Length=349, Percent_Identity=28.9398280802292, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21357035, Length=349, Percent_Identity=28.9398280802292, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28574430, Length=144, Percent_Identity=28.4722222222222, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24584409, Length=144, Percent_Identity=28.4722222222222, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585900, Length=180, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585898, Length=180, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585896, Length=180, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921672, Length=180, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24645863, Length=109, Percent_Identity=35.7798165137615, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 37047; Mature: 37047

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAHADTHLDQSITGRFRYAIILTAITLVAELAGGLWTNSLALLSDAAHVFLDLFALLLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGAIKLASYPVSETRTFGWHRMEVFASFINGSTVFLIAGIISYEALVRFIHPEEVKSLEM
HCHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LIIAFVGLVMNLIAAGALHSHSHDDLNVHSAFLHVIGDAAASVGVIIGGIIMYFTNWYLL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVISIGIGFIIFWGSWRVMRESVHILLEGVPRGVEVEQVSAAILEVEGVEEVHHVNIWT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
ICSHILALSGHIVVPPTFKGEHGPILRRIEERLFERFHISHTTLQLETTRCTDTEGAKQF
HHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
RHRPRAASLAHAHAHPHHHPHGTCQGGHHHHDHTP
HCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHAHADTHLDQSITGRFRYAIILTAITLVAELAGGLWTNSLALLSDAAHVFLDLFALLLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGAIKLASYPVSETRTFGWHRMEVFASFINGSTVFLIAGIISYEALVRFIHPEEVKSLEM
HCHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LIIAFVGLVMNLIAAGALHSHSHDDLNVHSAFLHVIGDAAASVGVIIGGIIMYFTNWYLL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAVISIGIGFIIFWGSWRVMRESVHILLEGVPRGVEVEQVSAAILEVEGVEEVHHVNIWT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
ICSHILALSGHIVVPPTFKGEHGPILRRIEERLFERFHISHTTLQLETTRCTDTEGAKQF
HHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
RHRPRAASLAHAHAHPHHHPHGTCQGGHHHHDHTP
HCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377; 9099864 [H]