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The map label for this gene is argG

Identifier: 197119999

GI number: 197119999

Start: 4161970

End: 4163190

Strand: Direct

Name: argG

Synonym: Gbem_3638

Alternate gene names: 197119999

Gene position: 4161970-4163190 (Clockwise)

Preceding gene: 197119998

Following gene: 197120000

Centisome position: 90.18

GC content: 60.36

Gene sequence:

>1221_bases
ATGGCAAAGAAAGAAGTGAAAAAGATCGTCCTCGCCTACTCCGGCGGGCTTGACACCTCCATCATCCTCAAATGGCTCAA
AAACGAGTACGGCTGCGAAGTGGTCACCTTCTCCGCTGACCTGGGACAGGGAGACGAGCTGGAGCCGGTTCGCGAGAAGG
CGTTCAAGACGGGCGCCGACAAGGTCTACATCGACGACCTGCGCGAAGAGTTCGTGCGCGACTTCGTGTACCCGATGTTC
CGCGCCAACGCGATCTACGAAGGGTCTTATCTCCTCGGCACCTCCATAGCGCGCCCGCTGATCGCTAAGCGCCAGATGGA
AATCGCCAAGATCGAAGGGTGCGACGCGGTCTCTCACGGCGCCACCGGCAAGGGTAACGACCAGGTTCGCTTCGAGCTCG
CCTACTACCACTTCAACCCCGGCATCACCGTCGTGGCCCCGTGGAGGGAATGGAAGCTCAACTCCCGCCAGGCGCTGATC
AACTACGCGAAGAGAAACGACATCCCGATCCCGATCACCAAGAAGCGCCCCTGGTCTTCCGACCGGAACCTTTTGCACAT
CTCCTTCGAAGGCGGCATCCTGGAGGATACCTGGCTGGAGCCCCCCGAGAACATGTTCGTGCTGACCAAGGCTCCGGAGA
AGGCGCCGAACAAGCCGCAGTACATCGAGATCGAGTTCGAAAAAGGGAACGCGGTGGCTGTCGACGGCGTGCGCATGTCC
CCGGCTGAGCTTTTGGCTCACCTGAACACCATCGGCGGCGAGCACGGCATCGGCCGCGTCGACCTCCTGGAGAACCGTTC
GGTCGGCATGAAGTCCCGCGGCGTGTACGAGACCCCGGGCGGCACCATCCTGCGCGAGGCGCACATGGCCGTCGAGCAGA
TCACCATGGACCGTGAAGTCATGCACCTGAGGGACTCCCTGATCCCGCGCTACGCAGAGATGATCTACAACGGCTACTGG
TTCTCACCGGAGCGCGAGATGATGCAGTGCATGATCGACGAATCCCAGAAGACCGTGAACGGCGTGGCAAGGCTGAAGCT
CTACAAAGGTCACTGCCGCACCGTAGGCAGGAAATCCGAGAGCGACTCGCTCTTCAACCTCGACTTCGCCACCTTCGAGA
AGGATCAGGTCTACAACCAGGCGGACGCCGAGGGCTTCATCAAGCTGAACTCCCTGAGGCTCAGGATCCGTTCGCTCATG
CTGGCCAACAAGAACAAGTAA

Upstream 100 bases:

>100_bases
AGAACCGGCTGCACGTGCAAAAGGCCATCATGGCCATTTTGATGAAATAATCGGACCGATCCAAACGATCCGACCGATCA
CACAAAGATAAGGAGAACCC

Downstream 100 bases:

>100_bases
GGCAACTGGCAGTACAGGAGGGTCCATAGGGGCACGAAACCTTTCGTGCCCCTATCTCGCTTTATATGGAGCAAAACGAG
TTCAAGGCGGGCCATATTGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MAKKEVKKIVLAYSGGLDTSIILKWLKNEYGCEVVTFSADLGQGDELEPVREKAFKTGADKVYIDDLREEFVRDFVYPMF
RANAIYEGSYLLGTSIARPLIAKRQMEIAKIEGCDAVSHGATGKGNDQVRFELAYYHFNPGITVVAPWREWKLNSRQALI
NYAKRNDIPIPITKKRPWSSDRNLLHISFEGGILEDTWLEPPENMFVLTKAPEKAPNKPQYIEIEFEKGNAVAVDGVRMS
PAELLAHLNTIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREVMHLRDSLIPRYAEMIYNGYW
FSPEREMMQCMIDESQKTVNGVARLKLYKGHCRTVGRKSESDSLFNLDFATFEKDQVYNQADAEGFIKLNSLRLRIRSLM
LANKNK

Sequences:

>Translated_406_residues
MAKKEVKKIVLAYSGGLDTSIILKWLKNEYGCEVVTFSADLGQGDELEPVREKAFKTGADKVYIDDLREEFVRDFVYPMF
RANAIYEGSYLLGTSIARPLIAKRQMEIAKIEGCDAVSHGATGKGNDQVRFELAYYHFNPGITVVAPWREWKLNSRQALI
NYAKRNDIPIPITKKRPWSSDRNLLHISFEGGILEDTWLEPPENMFVLTKAPEKAPNKPQYIEIEFEKGNAVAVDGVRMS
PAELLAHLNTIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREVMHLRDSLIPRYAEMIYNGYW
FSPEREMMQCMIDESQKTVNGVARLKLYKGHCRTVGRKSESDSLFNLDFATFEKDQVYNQADAEGFIKLNSLRLRIRSLM
LANKNK
>Mature_405_residues
AKKEVKKIVLAYSGGLDTSIILKWLKNEYGCEVVTFSADLGQGDELEPVREKAFKTGADKVYIDDLREEFVRDFVYPMFR
ANAIYEGSYLLGTSIARPLIAKRQMEIAKIEGCDAVSHGATGKGNDQVRFELAYYHFNPGITVVAPWREWKLNSRQALIN
YAKRNDIPIPITKKRPWSSDRNLLHISFEGGILEDTWLEPPENMFVLTKAPEKAPNKPQYIEIEFEKGNAVAVDGVRMSP
AELLAHLNTIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREVMHLRDSLIPRYAEMIYNGYWF
SPEREMMQCMIDESQKTVNGVARLKLYKGHCRTVGRKSESDSLFNLDFATFEKDQVYNQADAEGFIKLNSLRLRIRSLML
ANKNK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=396, Percent_Identity=49.4949494949495, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI16950633, Length=396, Percent_Identity=49.4949494949495, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1789563, Length=409, Percent_Identity=24.9388753056235, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324514, Length=402, Percent_Identity=43.0348258706468, Blast_Score=358, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21358151, Length=399, Percent_Identity=43.609022556391, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_GEOBB (B5ED13)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002140426.1
- ProteinModelPortal:   B5ED13
- SMR:   B5ED13
- GeneID:   6780502
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_3638
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 46170; Mature: 46038

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 39-39 BINDING 90-90 BINDING 95-95 BINDING 120-120 BINDING 122-122 BINDING 126-126 BINDING 126-126 BINDING 127-127 BINDING 130-130 BINDING 179-179 BINDING 188-188 BINDING 264-264 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKEVKKIVLAYSGGLDTSIILKWLKNEYGCEVVTFSADLGQGDELEPVREKAFKTGAD
CCHHHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCC
KVYIDDLREEFVRDFVYPMFRANAIYEGSYLLGTSIARPLIAKRQMEIAKIEGCDAVSHG
EEEHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHHEECCCCCCCCC
ATGKGNDQVRFELAYYHFNPGITVVAPWREWKLNSRQALINYAKRNDIPIPITKKRPWSS
CCCCCCCEEEEEEEEEEECCCEEEEECHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
DRNLLHISFEGGILEDTWLEPPENMFVLTKAPEKAPNKPQYIEIEFEKGNAVAVDGVRMS
CCCEEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCCC
PAELLAHLNTIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREV
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
MHLRDSLIPRYAEMIYNGYWFSPEREMMQCMIDESQKTVNGVARLKLYKGHCRTVGRKSE
HHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHCCCCCC
SDSLFNLDFATFEKDQVYNQADAEGFIKLNSLRLRIRSLMLANKNK
CCCEEEEEHHHCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKKEVKKIVLAYSGGLDTSIILKWLKNEYGCEVVTFSADLGQGDELEPVREKAFKTGAD
CHHHHHEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCC
KVYIDDLREEFVRDFVYPMFRANAIYEGSYLLGTSIARPLIAKRQMEIAKIEGCDAVSHG
EEEHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHHEECCCCCCCCC
ATGKGNDQVRFELAYYHFNPGITVVAPWREWKLNSRQALINYAKRNDIPIPITKKRPWSS
CCCCCCCEEEEEEEEEEECCCEEEEECHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
DRNLLHISFEGGILEDTWLEPPENMFVLTKAPEKAPNKPQYIEIEFEKGNAVAVDGVRMS
CCCEEEEEECCCEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCCC
PAELLAHLNTIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREV
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
MHLRDSLIPRYAEMIYNGYWFSPEREMMQCMIDESQKTVNGVARLKLYKGHCRTVGRKSE
HHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHCCCCCC
SDSLFNLDFATFEKDQVYNQADAEGFIKLNSLRLRIRSLMLANKNK
CCCEEEEEHHHCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA