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The map label for this gene is irlS [H]

Identifier: 197119977

GI number: 197119977

Start: 4134840

End: 4136309

Strand: Direct

Name: irlS [H]

Synonym: Gbem_3616

Alternate gene names: 197119977

Gene position: 4134840-4136309 (Clockwise)

Preceding gene: 197119976

Following gene: 197119978

Centisome position: 89.59

GC content: 66.19

Gene sequence:

>1470_bases
ATGTCCTCGACGAGACCGCCTGAACCCCGCCGCTCCACCTCCATCACCGCGCGCCTGGTCGCCTTCTACCTGATCAGCAC
CCTTTTGATACTCCTTTGCACCAACTGGTTCCAGTTCAAGGCGCTCTCCAAGGACCTCAACTACGAGGACAACGATTTCG
TGCTGGAAAGGATCAACTCGCTGCGCGACATCGTGGCCCGGCACCCGGACGCGCTGAGGGACCAGATACCGATCGACAGG
CCGGGGCAGCCCGTGCGGCACCTGGTTCGGATCCAGGACGCGGAGGGGCGCACCCTGCTGGAGTCGCCGCAGATGTCGGA
GTTGGCGGTGGGGCTTTTCCCGCCGGCGGTGACCGCGCAGGAAAACATCGGCCGCAGCGCCAAGTACCGCGCGTCCAATC
ACAGGCGCTACCTCCTGAACGCTGCCTGGGCCGGGAAGGAGGGCGATCCGCACTTCCGGCTGTTGCAGGTGGCGCTGGAC
ATAACCAATGAAGACGCCCTGATTTCCAAGTACCTGCTGCGTACCGTGGCCGCGGTGGCCATAGGGCTGCTGCTCGCTGC
GGGGCTCGGGGTCCTCATCGCCCGCCGGGGCTTGAGGCCGCTGCAGGAGATGGCGGCAAAGGTGGCCCGGATCACCGAGG
CCGACCTGCACCAACGGGTCGGGACGACGACGAGCTGGCCTAACGAGCTGGACCAGTTGACCGCGGCTCTCGACGCCATG
CTGGCCCGGCTGGAGGAATCCTTCGCACGGCTGTCGGAGTTTTCAGCAAACCTGGCCCACGAGCTGCGCACGCCGATCAA
CAACCTGCGCGGCGAAGCCGAGGTCGCGCTGTCCCGGGCACGCTCCGAAGAGGAATACCGTCGCATCATCGAGTCGAGCA
TCGAGGAGTACGAGCGGCTGGCCCGCATGGTGGGGGACATCCTGTTCCTGGCCCGGCCGGACCGCGCCCACGAAAAGCGC
CTGATCGACGCGAGGCAGGCCTTGGAAACGCTGGCGGAGTACTACAGCAACGTTGCGGAGGAGCAGCAGACTTCCATCTC
GGTGGAGGGGAGCGGCGACATCTGCGTAGACCCCAACCTGTTCCAACGCGCCGTGGGGAACCTCATCTCGAACGCGCTGC
ACTACACCCCGAAGTCCGGGCGCATCTCCCTGAAGATCGACCATAAGGAGGACGGCTCGGTCGCCATCTCCGTCGCCGAC
AACGGCATGGGGGTGGACGAAGCGGAGCTGCCGCGCGTCTTCGATCGCTTCTACCGCTCGCCGCAGGCCCGCCTGGTGTA
CAACCAGGGGAGCGGTCTCGGGCTCGCCATCGTCCGTTCCATCATGACCCTGCATGGCGGCAGCGTCTCTGTCGCCAGCG
CTCCCGGGATAGGGACCACCGTCACCCTCAGTTTTGCACCTCCCCCGCCGGGTGCAGTTGCAGAACAAGTCCCCCCCTCC
CTTGACGGGAGGGGGACGGGGGGGGGGTGA

Upstream 100 bases:

>100_bases
ACCGACACCAACGTGGTGGACGTGGCGATCAGAAGGCTCAGGAAGAAGGTCGACGACCCCTTCCCGGTCAAACTCATCCA
AACCATCAGGGGAGTAGGCT

Downstream 100 bases:

>100_bases
AGCACCGGCAGCCGGCGCCATACATCTTTTTCACGAGACTTTTCCCCGCAATTTGGCTAGCATGGCCCACTTGAAGCTAA
GCAATAAGACCGCCGCCACA

Product: HAMP domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSSTRPPEPRRSTSITARLVAFYLISTLLILLCTNWFQFKALSKDLNYEDNDFVLERINSLRDIVARHPDALRDQIPIDR
PGQPVRHLVRIQDAEGRTLLESPQMSELAVGLFPPAVTAQENIGRSAKYRASNHRRYLLNAAWAGKEGDPHFRLLQVALD
ITNEDALISKYLLRTVAAVAIGLLLAAGLGVLIARRGLRPLQEMAAKVARITEADLHQRVGTTTSWPNELDQLTAALDAM
LARLEESFARLSEFSANLAHELRTPINNLRGEAEVALSRARSEEEYRRIIESSIEEYERLARMVGDILFLARPDRAHEKR
LIDARQALETLAEYYSNVAEEQQTSISVEGSGDICVDPNLFQRAVGNLISNALHYTPKSGRISLKIDHKEDGSVAISVAD
NGMGVDEAELPRVFDRFYRSPQARLVYNQGSGLGLAIVRSIMTLHGGSVSVASAPGIGTTVTLSFAPPPPGAVAEQVPPS
LDGRGTGGG

Sequences:

>Translated_489_residues
MSSTRPPEPRRSTSITARLVAFYLISTLLILLCTNWFQFKALSKDLNYEDNDFVLERINSLRDIVARHPDALRDQIPIDR
PGQPVRHLVRIQDAEGRTLLESPQMSELAVGLFPPAVTAQENIGRSAKYRASNHRRYLLNAAWAGKEGDPHFRLLQVALD
ITNEDALISKYLLRTVAAVAIGLLLAAGLGVLIARRGLRPLQEMAAKVARITEADLHQRVGTTTSWPNELDQLTAALDAM
LARLEESFARLSEFSANLAHELRTPINNLRGEAEVALSRARSEEEYRRIIESSIEEYERLARMVGDILFLARPDRAHEKR
LIDARQALETLAEYYSNVAEEQQTSISVEGSGDICVDPNLFQRAVGNLISNALHYTPKSGRISLKIDHKEDGSVAISVAD
NGMGVDEAELPRVFDRFYRSPQARLVYNQGSGLGLAIVRSIMTLHGGSVSVASAPGIGTTVTLSFAPPPPGAVAEQVPPS
LDGRGTGGG
>Mature_488_residues
SSTRPPEPRRSTSITARLVAFYLISTLLILLCTNWFQFKALSKDLNYEDNDFVLERINSLRDIVARHPDALRDQIPIDRP
GQPVRHLVRIQDAEGRTLLESPQMSELAVGLFPPAVTAQENIGRSAKYRASNHRRYLLNAAWAGKEGDPHFRLLQVALDI
TNEDALISKYLLRTVAAVAIGLLLAAGLGVLIARRGLRPLQEMAAKVARITEADLHQRVGTTTSWPNELDQLTAALDAML
ARLEESFARLSEFSANLAHELRTPINNLRGEAEVALSRARSEEEYRRIIESSIEEYERLARMVGDILFLARPDRAHEKRL
IDARQALETLAEYYSNVAEEQQTSISVEGSGDICVDPNLFQRAVGNLISNALHYTPKSGRISLKIDHKEDGSVAISVADN
GMGVDEAELPRVFDRFYRSPQARLVYNQGSGLGLAIVRSIMTLHGGSVSVASAPGIGTTVTLSFAPPPPGAVAEQVPPSL
DGRGTGGG

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=479, Percent_Identity=29.8538622129436, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788279, Length=287, Percent_Identity=34.1463414634146, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=27.6752767527675, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790861, Length=227, Percent_Identity=29.9559471365639, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=26.271186440678, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=28.7671232876712, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788549, Length=228, Percent_Identity=27.6315789473684, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790346, Length=315, Percent_Identity=25.3968253968254, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=28.3783783783784, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693157, Length=240, Percent_Identity=27.5, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790551, Length=324, Percent_Identity=27.4691358024691, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=25.9414225941423, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789403, Length=309, Percent_Identity=28.8025889967638, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789149, Length=278, Percent_Identity=23.7410071942446, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786912, Length=247, Percent_Identity=27.5303643724696, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082128, Length=282, Percent_Identity=24.113475177305, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787894, Length=295, Percent_Identity=26.4406779661017, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87081816, Length=252, Percent_Identity=28.1746031746032, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787374, Length=289, Percent_Identity=24.9134948096886, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53680; Mature: 53549

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTRPPEPRRSTSITARLVAFYLISTLLILLCTNWFQFKALSKDLNYEDNDFVLERINS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHH
LRDIVARHPDALRDQIPIDRPGQPVRHLVRIQDAEGRTLLESPQMSELAVGLFPPAVTAQ
HHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHCCCCHHHHHHHCCCCCCCCH
ENIGRSAKYRASNHRRYLLNAAWAGKEGDPHFRLLQVALDITNEDALISKYLLRTVAAVA
HHCCCCHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IGLLLAAGLGVLIARRGLRPLQEMAAKVARITEADLHQRVGTTTSWPNELDQLTAALDAM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LARLEESFARLSEFSANLAHELRTPINNLRGEAEVALSRARSEEEYRRIIESSIEEYERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ARMVGDILFLARPDRAHEKRLIDARQALETLAEYYSNVAEEQQTSISVEGSGDICVDPNL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCHH
FQRAVGNLISNALHYTPKSGRISLKIDHKEDGSVAISVADNGMGVDEAELPRVFDRFYRS
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
PQARLVYNQGSGLGLAIVRSIMTLHGGSVSVASAPGIGTTVTLSFAPPPPGAVAEQVPPS
CCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCCCHHHHCCCC
LDGRGTGGG
CCCCCCCCC
>Mature Secondary Structure 
SSTRPPEPRRSTSITARLVAFYLISTLLILLCTNWFQFKALSKDLNYEDNDFVLERINS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHH
LRDIVARHPDALRDQIPIDRPGQPVRHLVRIQDAEGRTLLESPQMSELAVGLFPPAVTAQ
HHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHCCCCHHHHHHHCCCCCCCCH
ENIGRSAKYRASNHRRYLLNAAWAGKEGDPHFRLLQVALDITNEDALISKYLLRTVAAVA
HHCCCCHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IGLLLAAGLGVLIARRGLRPLQEMAAKVARITEADLHQRVGTTTSWPNELDQLTAALDAM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LARLEESFARLSEFSANLAHELRTPINNLRGEAEVALSRARSEEEYRRIIESSIEEYERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ARMVGDILFLARPDRAHEKRLIDARQALETLAEYYSNVAEEQQTSISVEGSGDICVDPNL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCHH
FQRAVGNLISNALHYTPKSGRISLKIDHKEDGSVAISVADNGMGVDEAELPRVFDRFYRS
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
PQARLVYNQGSGLGLAIVRSIMTLHGGSVSVASAPGIGTTVTLSFAPPPPGAVAEQVPPS
CCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCCCCHHHHCCCC
LDGRGTGGG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]