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The map label for this gene is yafV [C]

Identifier: 197119871

GI number: 197119871

Start: 4021724

End: 4022503

Strand: Direct

Name: yafV [C]

Synonym: Gbem_3509

Alternate gene names: 197119871

Gene position: 4021724-4022503 (Clockwise)

Preceding gene: 197119863

Following gene: 197119872

Centisome position: 87.14

GC content: 64.87

Gene sequence:

>780_bases
ATGCAAAAAAGCGTCAAGGCTGCTGCGGTCCAATTCAACATCGCGCTTGCGGACGTGGACCAAAACCTCGCCTATGCCGA
AAAAGCGCTGCGCCGCCTGGCCGCCCAGGGGGTGGAGCTGGCCGTATTGCCGGAAATGTGGAGCTGCGGCTTCGCCTACC
GGGAGCTGAACCAGCTGGCCACGCGCACCCCGGGGATCGTCGAGAAGCTGCAGGCGCTCTCAGCCGAACTCTCCCTGACC
GTCATCGGCAGCCTGCCTGAGCCGCACGGAGACAAGGTCTTCAACACCGCCTACGTAGTGGACCGCGGCAGGGTCGCAGG
AAGCTACCGCAAGATGCACCTCTTCTCGCTGATGGGGGAGGACCGCCACCTGGATCGCGGCGACTCGATCCTTTTGGCGC
AGACCAGCGTGGGGAGGATCGGCGTCATGATCTGCTACGACCTCCGCTTCCCGGAACTGGCGCGCAGGCTGGCGGTCGAC
GGCGCCGACATCATCGTCGTCCCCGGTGAGTGGCCCAAGCCGCGCGAAGAGCACTGGCGGGGACTTTTGAAGGGTCGCGC
CATAGAGAACCAGCTCTTCGTGATAGCGGCCAACGCCTGCGGGGTCATCGGCAAACTCGACTTCTTCGGCTCCAGCATGA
TCCTCGGCCCCAAGGGGGAGGTCCTCGCGGAGGCGGGGTACGAGAACGCGGAGCCGACGGCTCTGCTCGACCCGGCCGAG
ATGGAAAAGTGGCGCCAGAGCATAACCTGCTTCCAGGACCGGCGCCCGGAGTGCTATTAA

Upstream 100 bases:

>100_bases
CAGGGACTCGGATTACACTCCGAAACTCGCCGACCGCCGGTGGCCGCCATGTCCGGCCAGACTTGACATGCGGCGATTCT
TTTCCTGAAGGAGGGAGAAC

Downstream 100 bases:

>100_bases
ATAGAGCCCGGGGGCTGTGAAGGGTAACCCCGCGCCCCCGTGCGCGCTTTCTTTACAGTTGACTTCGCATTGCGTAAAGC
ATATTTTCCCTGTTTGATCA

Product: nitrilase/amidohydrolase superfamily protein

Products: NA

Alternate protein names: ORF 5 [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MQKSVKAAAVQFNIALADVDQNLAYAEKALRRLAAQGVELAVLPEMWSCGFAYRELNQLATRTPGIVEKLQALSAELSLT
VIGSLPEPHGDKVFNTAYVVDRGRVAGSYRKMHLFSLMGEDRHLDRGDSILLAQTSVGRIGVMICYDLRFPELARRLAVD
GADIIVVPGEWPKPREEHWRGLLKGRAIENQLFVIAANACGVIGKLDFFGSSMILGPKGEVLAEAGYENAEPTALLDPAE
MEKWRQSITCFQDRRPECY

Sequences:

>Translated_259_residues
MQKSVKAAAVQFNIALADVDQNLAYAEKALRRLAAQGVELAVLPEMWSCGFAYRELNQLATRTPGIVEKLQALSAELSLT
VIGSLPEPHGDKVFNTAYVVDRGRVAGSYRKMHLFSLMGEDRHLDRGDSILLAQTSVGRIGVMICYDLRFPELARRLAVD
GADIIVVPGEWPKPREEHWRGLLKGRAIENQLFVIAANACGVIGKLDFFGSSMILGPKGEVLAEAGYENAEPTALLDPAE
MEKWRQSITCFQDRRPECY
>Mature_259_residues
MQKSVKAAAVQFNIALADVDQNLAYAEKALRRLAAQGVELAVLPEMWSCGFAYRELNQLATRTPGIVEKLQALSAELSLT
VIGSLPEPHGDKVFNTAYVVDRGRVAGSYRKMHLFSLMGEDRHLDRGDSILLAQTSVGRIGVMICYDLRFPELARRLAVD
GADIIVVPGEWPKPREEHWRGLLKGRAIENQLFVIAANACGVIGKLDFFGSSMILGPKGEVLAEAGYENAEPTALLDPAE
MEKWRQSITCFQDRRPECY

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=268, Percent_Identity=32.8358208955224, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI297632350, Length=265, Percent_Identity=32.8301886792453, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI297632348, Length=265, Percent_Identity=32.8301886792453, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI5031947, Length=265, Percent_Identity=32.8301886792453, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1786412, Length=251, Percent_Identity=29.4820717131474, Blast_Score=110, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17556280, Length=259, Percent_Identity=30.1158301158301, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323383, Length=279, Percent_Identity=29.0322580645161, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322335, Length=307, Percent_Identity=23.7785016286645, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17933642, Length=261, Percent_Identity=31.0344827586207, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355835, Length=234, Percent_Identity=28.2051282051282, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 28633; Mature: 28633

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSVKAAAVQFNIALADVDQNLAYAEKALRRLAAQGVELAVLPEMWSCGFAYRELNQLA
CCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHCCHHHHHHHHHH
TRTPGIVEKLQALSAELSLTVIGSLPEPHGDKVFNTAYVVDRGRVAGSYRKMHLFSLMGE
HCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCC
DRHLDRGDSILLAQTSVGRIGVMICYDLRFPELARRLAVDGADIIVVPGEWPKPREEHWR
CCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
GLLKGRAIENQLFVIAANACGVIGKLDFFGSSMILGPKGEVLAEAGYENAEPTALLDPAE
HHHHCCEECCEEEEEEECCCCCEECHHHCCCEEEECCCCCHHHHCCCCCCCCCEEECHHH
MEKWRQSITCFQDRRPECY
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQKSVKAAAVQFNIALADVDQNLAYAEKALRRLAAQGVELAVLPEMWSCGFAYRELNQLA
CCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHCCHHHHHHHHHH
TRTPGIVEKLQALSAELSLTVIGSLPEPHGDKVFNTAYVVDRGRVAGSYRKMHLFSLMGE
HCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCC
DRHLDRGDSILLAQTSVGRIGVMICYDLRFPELARRLAVDGADIIVVPGEWPKPREEHWR
CCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
GLLKGRAIENQLFVIAANACGVIGKLDFFGSSMILGPKGEVLAEAGYENAEPTALLDPAE
HHHHCCEECCEEEEEEECCCCCEECHHHCCCEEEECCCCCHHHHCCCCCCCCCEEECHHH
MEKWRQSITCFQDRRPECY
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8359673 [H]