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The map label for this gene is hpnA [H]

Identifier: 197119725

GI number: 197119725

Start: 3863541

End: 3864530

Strand: Direct

Name: hpnA [H]

Synonym: Gbem_3361

Alternate gene names: 197119725

Gene position: 3863541-3864530 (Clockwise)

Preceding gene: 197119724

Following gene: 197119726

Centisome position: 83.71

GC content: 64.04

Gene sequence:

>990_bases
ATGAAAGCATTCGTCACAGGCGCCACCGGGTTCATCGGCGCGAGCATCGTGCGCGAATTGTTGAAGGACGGCTGGGAGGT
ACGGGTGCTGGCCCGGCCCGGCTCGGACCGCCGCAACCTCTTCGGGCTCGACGTGGAGATCCGGGAAGGGGATCTGAGCG
ATCGCGAAGCGCTGGTGCAGGCGCTCAGCGGCTGTCAGGCGCTGTTCCACGCCGCCGCCGACTACCGGCTCTGGACACGC
ACGCCGCAGGCCATGTACGATGTCAACGTCAAAGGGACCCGGGCCATACTGTCGGCGGCTCTCGCGGCGGGGATCGAGAA
GGTGGTCTACACCAGCAGCGTCGGGACCCTGGGCACCCCCGGCGACGGCACCCCCGGCGACGAGGGCACCCCGGTGGATT
TTTGCCACATGGTGGGGGACTACAAAAAGAGCAAGTTCCTCGCCGAGCGGGCCGCGGAATCGTTTCTGGAGAAGGGGTTG
CCGCTCGTGATCGTGAACCCATCGACCCCGGTGGGGCCGATGGATGTGAAGCCTACGCCTACGGGAAAGATCATAGTCGA
CTTCCTGAACGGCCGGATGCCCGCCTACCTGGACACCGGGCTGAACCTGATAGACGTAGAGGCGTGCGCCCGGGGTCATA
TCCTGGCGGCGCAGAAGGGGCGAGTCGGGGAAAAGTACATCCTTGGCAACCGCAACCTGACCCTGGCCGAGATATTCGAG
ATTCTCTCCGGCATCACCGGTCTCAAGGCGCCGCGGGTGAAGCTCCCCTACTATCCGATACTCATGGCCGCCTATGCGAA
CCATGCACTGTCGGCCGTGACCGGGAAAGAGCCGCTGATACCGCTTGCCGGAGTGCAGATGGCAGCGAAGTTCATGTATT
TCGATTCGGGGAAGGCGGTGAGCGAGTTGGGGTTGCCGCTTTGTCCCGTGGAAGGCGCGCTGGAGCGAGCCGTAGAATGG
TTCCGCAGCAACGGCTACGTAAACCGGTGA

Upstream 100 bases:

>100_bases
TTCATGATCAAGTACCACATCTACCGCAACTGCTTCCCGCTCATGGCCCTGGGGACCTACCGCACCCTGACCAGGACGCA
GCCGTGACCGGAGCGCGCGG

Downstream 100 bases:

>100_bases
AGCAAAAGCCGTAGACGTTGGACATTGAGTTTCTTTTTTTGCATTGATATCGCTACGGGTTCTCAAGGAGATCCATGTAC
AAACTGCTAAGCAAGATCAA

Product: NAD-dependent nucleoside diphosphate-sugar epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKAFVTGATGFIGASIVRELLKDGWEVRVLARPGSDRRNLFGLDVEIREGDLSDREALVQALSGCQALFHAAADYRLWTR
TPQAMYDVNVKGTRAILSAALAAGIEKVVYTSSVGTLGTPGDGTPGDEGTPVDFCHMVGDYKKSKFLAERAAESFLEKGL
PLVIVNPSTPVGPMDVKPTPTGKIIVDFLNGRMPAYLDTGLNLIDVEACARGHILAAQKGRVGEKYILGNRNLTLAEIFE
ILSGITGLKAPRVKLPYYPILMAAYANHALSAVTGKEPLIPLAGVQMAAKFMYFDSGKAVSELGLPLCPVEGALERAVEW
FRSNGYVNR

Sequences:

>Translated_329_residues
MKAFVTGATGFIGASIVRELLKDGWEVRVLARPGSDRRNLFGLDVEIREGDLSDREALVQALSGCQALFHAAADYRLWTR
TPQAMYDVNVKGTRAILSAALAAGIEKVVYTSSVGTLGTPGDGTPGDEGTPVDFCHMVGDYKKSKFLAERAAESFLEKGL
PLVIVNPSTPVGPMDVKPTPTGKIIVDFLNGRMPAYLDTGLNLIDVEACARGHILAAQKGRVGEKYILGNRNLTLAEIFE
ILSGITGLKAPRVKLPYYPILMAAYANHALSAVTGKEPLIPLAGVQMAAKFMYFDSGKAVSELGLPLCPVEGALERAVEW
FRSNGYVNR
>Mature_329_residues
MKAFVTGATGFIGASIVRELLKDGWEVRVLARPGSDRRNLFGLDVEIREGDLSDREALVQALSGCQALFHAAADYRLWTR
TPQAMYDVNVKGTRAILSAALAAGIEKVVYTSSVGTLGTPGDGTPGDEGTPVDFCHMVGDYKKSKFLAERAAESFLEKGL
PLVIVNPSTPVGPMDVKPTPTGKIIVDFLNGRMPAYLDTGLNLIDVEACARGHILAAQKGRVGEKYILGNRNLTLAEIFE
ILSGITGLKAPRVKLPYYPILMAAYANHALSAVTGKEPLIPLAGVQMAAKFMYFDSGKAVSELGLPLCPVEGALERAVEW
FRSNGYVNR

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=345, Percent_Identity=28.9855072463768, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8393516, Length=345, Percent_Identity=28.9855072463768, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI310132178, Length=337, Percent_Identity=25.5192878338279, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI310113012, Length=337, Percent_Identity=25.5192878338279, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI239745448, Length=337, Percent_Identity=25.5192878338279, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI116268111, Length=360, Percent_Identity=25.2777777777778, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71987463, Length=336, Percent_Identity=28.2738095238095, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321437, Length=347, Percent_Identity=24.207492795389, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 35372; Mature: 35372

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFVTGATGFIGASIVRELLKDGWEVRVLARPGSDRRNLFGLDVEIREGDLSDREALVQ
CCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHH
ALSGCQALFHAAADYRLWTRTPQAMYDVNVKGTRAILSAALAAGIEKVVYTSSVGTLGTP
HHHHHHHHHHHHHCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GDGTPGDEGTPVDFCHMVGDYKKSKFLAERAAESFLEKGLPLVIVNPSTPVGPMDVKPTP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TGKIIVDFLNGRMPAYLDTGLNLIDVEACARGHILAAQKGRVGEKYILGNRNLTLAEIFE
CCCEEEEECCCCCCCHHHCCCCEEEHHHHCCCCEEEECCCCCCCEEEECCCCCCHHHHHH
ILSGITGLKAPRVKLPYYPILMAAYANHALSAVTGKEPLIPLAGVQMAAKFMYFDSGKAV
HHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCHH
SELGLPLCPVEGALERAVEWFRSNGYVNR
HHCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKAFVTGATGFIGASIVRELLKDGWEVRVLARPGSDRRNLFGLDVEIREGDLSDREALVQ
CCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHH
ALSGCQALFHAAADYRLWTRTPQAMYDVNVKGTRAILSAALAAGIEKVVYTSSVGTLGTP
HHHHHHHHHHHHHCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GDGTPGDEGTPVDFCHMVGDYKKSKFLAERAAESFLEKGLPLVIVNPSTPVGPMDVKPTP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
TGKIIVDFLNGRMPAYLDTGLNLIDVEACARGHILAAQKGRVGEKYILGNRNLTLAEIFE
CCCEEEEECCCCCCCHHHCCCCEEEHHHHCCCCEEEECCCCCCCEEEECCCCCCHHHHHH
ILSGITGLKAPRVKLPYYPILMAAYANHALSAVTGKEPLIPLAGVQMAAKFMYFDSGKAV
HHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCHH
SELGLPLCPVEGALERAVEWFRSNGYVNR
HHCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]