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The map label for this gene is nrfC [H]

Identifier: 197119565

GI number: 197119565

Start: 3684596

End: 3685378

Strand: Direct

Name: nrfC [H]

Synonym: Gbem_3195

Alternate gene names: 197119565

Gene position: 3684596-3685378 (Clockwise)

Preceding gene: 197119564

Following gene: 197119566

Centisome position: 79.84

GC content: 63.09

Gene sequence:

>783_bases
ATGATCGACAGAAGAACTTTTTGCAAGAACACGCTGCTCATCGCCGGGGGCCTCGCGCTCCCGCTCTCGGCGCTGGAACT
CTTCGACCCGAAGCGGCTTTTGGCCGAGAAGGACCAAAAAGGGGCGCGCTGGGTGTTCCTGGTGGACACCCGCAAATGCG
TCGGCTGCGGTTTCTGCGTCAAGGGGTGCAAGGTGGAGAACGAGGTTCCCTATGACGCCAACGTGACCCGCACCTGGGTC
GAGCGCTACCTGGTCACCAGCGACAGCAAGACCCACATCGACTCCCCCAAGGGGGCGCGCGACGGCTTCCCAAGGAAGGA
GGTCGAGATAGGGCACGGAAAGCTGGAGGAGGTCCGCGACGAGGATCTCGAGAAGGCGTTCTTCGTCCCCAAGCTCTGCA
ACCAGTGCGACAATCCCCCCTGCGTCCAGGTCTGCCCGGTGGGGGCGACCTACCAAACCGAGGACGGTGTGGTGCTGGTG
GACCGTAGCTGGTGCATCGGCTGCGGCTACTGCATCATGGGGTGCCCATACGGGGTCCGCTTCTTCCACCCGGTGTACCA
CGTGGCCGACAAGTGCAACTTCTGCTACCACCGGATCAGCAAGGGGATGCAGACCGCCTGCGTGGACAGCTGCGCTTTCG
GGGCCAGGCGCATCGGGAACCTCAGGGACCCCGAGGACCCGGTCACCAAGGTGATCATGACCGAGCGGGTCAACGTCCTC
AAAGAGGAGTACGGCACCAAGCCGCAGGTCTTCTACCTGGGGCTTTCCAAGGAGGTGAAGTAG

Upstream 100 bases:

>100_bases
GCGGCGTCATGAAGGGGGTCGATCCGAAGACGCACCACGTAGGCCTTGACTGCGTCACCTGCCATTGGCCGCACGACCCG
AGGAAGGAGGGGCACCAGAG

Downstream 100 bases:

>100_bases
CCATGATGGTGCACGGAGAAGCCTGGACCCTCAAAGAATTCTTCACCTACCCCAACGAGTACATCTACTGGACCATCCAG
ATCGTGATGTATCCCTTCAT

Product: oxidoreductase iron-sulfur cluster-binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MIDRRTFCKNTLLIAGGLALPLSALELFDPKRLLAEKDQKGARWVFLVDTRKCVGCGFCVKGCKVENEVPYDANVTRTWV
ERYLVTSDSKTHIDSPKGARDGFPRKEVEIGHGKLEEVRDEDLEKAFFVPKLCNQCDNPPCVQVCPVGATYQTEDGVVLV
DRSWCIGCGYCIMGCPYGVRFFHPVYHVADKCNFCYHRISKGMQTACVDSCAFGARRIGNLRDPEDPVTKVIMTERVNVL
KEEYGTKPQVFYLGLSKEVK

Sequences:

>Translated_260_residues
MIDRRTFCKNTLLIAGGLALPLSALELFDPKRLLAEKDQKGARWVFLVDTRKCVGCGFCVKGCKVENEVPYDANVTRTWV
ERYLVTSDSKTHIDSPKGARDGFPRKEVEIGHGKLEEVRDEDLEKAFFVPKLCNQCDNPPCVQVCPVGATYQTEDGVVLV
DRSWCIGCGYCIMGCPYGVRFFHPVYHVADKCNFCYHRISKGMQTACVDSCAFGARRIGNLRDPEDPVTKVIMTERVNVL
KEEYGTKPQVFYLGLSKEVK
>Mature_260_residues
MIDRRTFCKNTLLIAGGLALPLSALELFDPKRLLAEKDQKGARWVFLVDTRKCVGCGFCVKGCKVENEVPYDANVTRTWV
ERYLVTSDSKTHIDSPKGARDGFPRKEVEIGHGKLEEVRDEDLEKAFFVPKLCNQCDNPPCVQVCPVGATYQTEDGVVLV
DRSWCIGCGYCIMGCPYGVRFFHPVYHVADKCNFCYHRISKGMQTACVDSCAFGARRIGNLRDPEDPVTKVIMTERVNVL
KEEYGTKPQVFYLGLSKEVK

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=214, Percent_Identity=34.1121495327103, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI226510944, Length=195, Percent_Identity=33.8461538461538, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787872, Length=213, Percent_Identity=28.6384976525822, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787122, Length=214, Percent_Identity=27.5700934579439, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787740, Length=111, Percent_Identity=41.4414414414414, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787478, Length=110, Percent_Identity=40.9090909090909, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790326, Length=196, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787749, Length=178, Percent_Identity=29.7752808988764, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789370, Length=181, Percent_Identity=28.1767955801105, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082180, Length=90, Percent_Identity=42.2222222222222, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082179, Length=102, Percent_Identity=35.2941176470588, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29264; Mature: 29264

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
6.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRRTFCKNTLLIAGGLALPLSALELFDPKRLLAEKDQKGARWVFLVDTRKCVGCGFCV
CCCCHHHHCCEEEEECCHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCHHCCCHHH
KGCKVENEVPYDANVTRTWVERYLVTSDSKTHIDSPKGARDGFPRKEVEIGHGKLEEVRD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHH
EDLEKAFFVPKLCNQCDNPPCVQVCPVGATYQTEDGVVLVDRSWCIGCGYCIMGCPYGVR
HHHHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCEEEEECCCEEECCHHHHCCCCCHH
FFHPVYHVADKCNFCYHRISKGMQTACVDSCAFGARRIGNLRDPEDPVTKVIMTERVNVL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
KEEYGTKPQVFYLGLSKEVK
HHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MIDRRTFCKNTLLIAGGLALPLSALELFDPKRLLAEKDQKGARWVFLVDTRKCVGCGFCV
CCCCHHHHCCEEEEECCHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEECCCHHCCCHHH
KGCKVENEVPYDANVTRTWVERYLVTSDSKTHIDSPKGARDGFPRKEVEIGHGKLEEVRD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHH
EDLEKAFFVPKLCNQCDNPPCVQVCPVGATYQTEDGVVLVDRSWCIGCGYCIMGCPYGVR
HHHHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCCEEEEECCCEEECCHHHHCCCCCHH
FFHPVYHVADKCNFCYHRISKGMQTACVDSCAFGARRIGNLRDPEDPVTKVIMTERVNVL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
KEEYGTKPQVFYLGLSKEVK
HHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]