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The map label for this gene is asnB-5 [H]

Identifier: 197118163

GI number: 197118163

Start: 2051103

End: 2052992

Strand: Direct

Name: asnB-5 [H]

Synonym: Gbem_1778

Alternate gene names: 197118163

Gene position: 2051103-2052992 (Clockwise)

Preceding gene: 197118162

Following gene: 197118164

Centisome position: 44.44

GC content: 64.87

Gene sequence:

>1890_bases
ATGTGTGGCATAGCCGGCTTCTTCAGAACCCTCTGTCCGGAGGGGGACACGCGCCTTTTGCGGCGGATGGGGGAGGTGAT
CAGCCACCGCGGTCCCGACGCCTCCGGCGAATTCCTCGATGAAGAGGTGGGGCTTTCCCACAGAAGGCTAAGCATCCTGG
ACCTTTCCCCCCTGGGGAACCAGCCGATGCACTCCCTGGACGGAAGGTACGTCATCGTCTTCAACGGCGAGATCTACAAC
TTCCTGGAATTGCGCGGGGAACTGGAGCGCGAAGGGGTCTCGTTCAGGAGCAGGACCGATACCGAGGTGATACTTGCCCT
CTACGCCAGGGAAGGGGTGGCGTGCTTGAAGCGCCTGAACGGCATGTTCGCTTTCGCCCTTTGGGACCGGGACACAAAGA
CGCTCCTTTTGGCTCGGGACCGGATCGGCAAGAAGCCTTTGTACTACTACCATGCCGGCGGGGATCGGATCGCCTTCGCC
TCGGAGATAAAGTCGCTCCTCGAGGTCCCGGGGGTGGAGCGGCAGGTTGAGCCGACGGCGGTGGCGGACTACCTGAAGTA
CCTCTACATCCCGGCTCCGAAGACCATCTACCGGCGTCTCTACAAGCTCCCCCCCGCCCACTGCCTGGAACTCAAGGCGG
GAGAAGAGCCACGGATCCGCGAGTATTGGGATGTGGAGTTCAAGGCCGACTACGGGCTGACCCCCGAGGCGGCGGAAGAA
GAGCTTTTGGACCTGCTGCAAAAGGCGACCGCCTGCCGCATGATCGCCGACGTGCCGCTGGGGGCCTTTCTTTCCGGCGG
GGTCGACTCCAGCGCCGTGGTGGCGCTCATGGCGCGCGCGGCGCAGGAGCCGGTGAAAACCTGCTCCATAGGCTTCGACG
ACAAGCGCCACGACGAGACCCCCTACGCCCGCGAGGTGGCACGGCTTTTTGCAACCGAGCACAAGGAGTACCGGGTCAGC
GCCGACCTGGCCGGGACGGTGGCCCTCTTGCCTCGCTTCTTCGATGAGCCGTTCGCCGACTCTTCGGCGCTTCCCACCTA
CCACGTCTCGCGCCTGGCGCGGCAGTCGGTGACGGTGGCGCTCGCTGGAGACGGCGGCGACGAGAGCTTCGCTGGGTACC
AGAAGTACGCCATCGAGCTGAAGGAGGATCTGGCGCGGCGCGCGGTCCCCAGGGCGCTTCTTTCCCTGATCGGCAAGGCG
GCAGGCGCCGCGTCGCACCCGCTTTTGAGAAAGGTGCGCTCCCTCACCACGAGCGCCCTTATGGAGCCGGCCGCAGCCTA
CTACCGGACCAACACCTTCGTGGAGGACGACCTTCTGGCGGAACTTTTGTCGGAGCCTTTCCGGCGGGCCTGCGCCGGTT
ACGACCCCGCCGAGCAGACCACGCGCTACTTCAGCCGGATGCAGGGGGCGGATCACGTGACCGCCATGCTCTACACCGAC
CTGAAGAGCTACCTCCCGGGGGACATCCTGGTCAAGGTGGACCGGATGAGCATGGCGCACTCGCTCGAGGTGAGGGCCCC
CTTTCTGGACTACCGGATCGTCGAGTTCGCCGCGAGGCTTCCCTCCAGCTGGAAGATCTCCGGCGGCGAGAAGAAGATCA
TCCTCAGGAAGGCCTTCGGGCGCCTGCTCCCGGACAGCGTGCTGAACCGCCCCAAGCAGGGGTTCACCGTGCCGCTGGAC
ACCTGGTTCAAAAAGGAGCTCAGGGAATTCGCCGGCAGCCGGCTTTTGGACGACCCGACGCTGTCGCAGTACCTCTCCCT
GAACGTGGTGAGGCGGGTCTGGGAGGAGCACCAGCAGGACAGGGCCCGGCACGGCACGCTCCTTTGGAGCCTGTTGATGC
TGGCGCTCTGGCACCAGGAGTACCAGGGAGGCGCACCATGTTCGAAGTGA

Upstream 100 bases:

>100_bases
TTTGCCACTACTTCTATATGCTGATGGCCGCCTCGCTGCTCGGGGGGTACCGCGGTGTTACCGGTAGACAGAAGGTGACC
TGGCGTGGGGGGACGGCCTG

Downstream 100 bases:

>100_bases
TCAGAAAGGACCTGTCCCGGCTCGACCCTGAGGGCAGCGCCTCGCCGAGGACGCTCGTAGCGGGGCTTCTCTCCCAGGGG
TTCCAGGCCATCCTCGTCTA

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MCGIAGFFRTLCPEGDTRLLRRMGEVISHRGPDASGEFLDEEVGLSHRRLSILDLSPLGNQPMHSLDGRYVIVFNGEIYN
FLELRGELEREGVSFRSRTDTEVILALYAREGVACLKRLNGMFAFALWDRDTKTLLLARDRIGKKPLYYYHAGGDRIAFA
SEIKSLLEVPGVERQVEPTAVADYLKYLYIPAPKTIYRRLYKLPPAHCLELKAGEEPRIREYWDVEFKADYGLTPEAAEE
ELLDLLQKATACRMIADVPLGAFLSGGVDSSAVVALMARAAQEPVKTCSIGFDDKRHDETPYAREVARLFATEHKEYRVS
ADLAGTVALLPRFFDEPFADSSALPTYHVSRLARQSVTVALAGDGGDESFAGYQKYAIELKEDLARRAVPRALLSLIGKA
AGAASHPLLRKVRSLTTSALMEPAAAYYRTNTFVEDDLLAELLSEPFRRACAGYDPAEQTTRYFSRMQGADHVTAMLYTD
LKSYLPGDILVKVDRMSMAHSLEVRAPFLDYRIVEFAARLPSSWKISGGEKKIILRKAFGRLLPDSVLNRPKQGFTVPLD
TWFKKELREFAGSRLLDDPTLSQYLSLNVVRRVWEEHQQDRARHGTLLWSLLMLALWHQEYQGGAPCSK

Sequences:

>Translated_629_residues
MCGIAGFFRTLCPEGDTRLLRRMGEVISHRGPDASGEFLDEEVGLSHRRLSILDLSPLGNQPMHSLDGRYVIVFNGEIYN
FLELRGELEREGVSFRSRTDTEVILALYAREGVACLKRLNGMFAFALWDRDTKTLLLARDRIGKKPLYYYHAGGDRIAFA
SEIKSLLEVPGVERQVEPTAVADYLKYLYIPAPKTIYRRLYKLPPAHCLELKAGEEPRIREYWDVEFKADYGLTPEAAEE
ELLDLLQKATACRMIADVPLGAFLSGGVDSSAVVALMARAAQEPVKTCSIGFDDKRHDETPYAREVARLFATEHKEYRVS
ADLAGTVALLPRFFDEPFADSSALPTYHVSRLARQSVTVALAGDGGDESFAGYQKYAIELKEDLARRAVPRALLSLIGKA
AGAASHPLLRKVRSLTTSALMEPAAAYYRTNTFVEDDLLAELLSEPFRRACAGYDPAEQTTRYFSRMQGADHVTAMLYTD
LKSYLPGDILVKVDRMSMAHSLEVRAPFLDYRIVEFAARLPSSWKISGGEKKIILRKAFGRLLPDSVLNRPKQGFTVPLD
TWFKKELREFAGSRLLDDPTLSQYLSLNVVRRVWEEHQQDRARHGTLLWSLLMLALWHQEYQGGAPCSK
>Mature_629_residues
MCGIAGFFRTLCPEGDTRLLRRMGEVISHRGPDASGEFLDEEVGLSHRRLSILDLSPLGNQPMHSLDGRYVIVFNGEIYN
FLELRGELEREGVSFRSRTDTEVILALYAREGVACLKRLNGMFAFALWDRDTKTLLLARDRIGKKPLYYYHAGGDRIAFA
SEIKSLLEVPGVERQVEPTAVADYLKYLYIPAPKTIYRRLYKLPPAHCLELKAGEEPRIREYWDVEFKADYGLTPEAAEE
ELLDLLQKATACRMIADVPLGAFLSGGVDSSAVVALMARAAQEPVKTCSIGFDDKRHDETPYAREVARLFATEHKEYRVS
ADLAGTVALLPRFFDEPFADSSALPTYHVSRLARQSVTVALAGDGGDESFAGYQKYAIELKEDLARRAVPRALLSLIGKA
AGAASHPLLRKVRSLTTSALMEPAAAYYRTNTFVEDDLLAELLSEPFRRACAGYDPAEQTTRYFSRMQGADHVTAMLYTD
LKSYLPGDILVKVDRMSMAHSLEVRAPFLDYRIVEFAARLPSSWKISGGEKKIILRKAFGRLLPDSVLNRPKQGFTVPLD
TWFKKELREFAGSRLLDDPTLSQYLSLNVVRRVWEEHQQDRARHGTLLWSLLMLALWHQEYQGGAPCSK

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=397, Percent_Identity=29.7229219143577, Blast_Score=113, Evalue=7e-25,
Organism=Homo sapiens, GI168229252, Length=397, Percent_Identity=29.7229219143577, Blast_Score=113, Evalue=7e-25,
Organism=Homo sapiens, GI168229250, Length=397, Percent_Identity=29.7229219143577, Blast_Score=113, Evalue=7e-25,
Organism=Homo sapiens, GI296010848, Length=370, Percent_Identity=30.2702702702703, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI296010852, Length=294, Percent_Identity=28.9115646258503, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI296010850, Length=294, Percent_Identity=28.9115646258503, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1786889, Length=416, Percent_Identity=29.8076923076923, Blast_Score=143, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71993933, Length=410, Percent_Identity=30.2439024390244, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25147557, Length=410, Percent_Identity=30.2439024390244, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI25147560, Length=354, Percent_Identity=30.5084745762712, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560178, Length=410, Percent_Identity=25.3658536585366, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321563, Length=414, Percent_Identity=28.2608695652174, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=416, Percent_Identity=27.6442307692308, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45553209, Length=397, Percent_Identity=30.4785894206549, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70718; Mature: 70718

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGFFRTLCPEGDTRLLRRMGEVISHRGPDASGEFLDEEVGLSHRRLSILDLSPLGN
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC
QPMHSLDGRYVIVFNGEIYNFLELRGELEREGVSFRSRTDTEVILALYAREGVACLKRLN
CCHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GMFAFALWDRDTKTLLLARDRIGKKPLYYYHAGGDRIAFASEIKSLLEVPGVERQVEPTA
CCEEEEEECCCCCEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHH
VADYLKYLYIPAPKTIYRRLYKLPPAHCLELKAGEEPRIREYWDVEFKADYGLTPEAAEE
HHHHHHHHCCCCCHHHHHHHHHCCCHHEEECCCCCCCCCHHHCCEEEECCCCCCCCHHHH
ELLDLLQKATACRMIADVPLGAFLSGGVDSSAVVALMARAAQEPVKTCSIGFDDKRHDET
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PYAREVARLFATEHKEYRVSADLAGTVALLPRFFDEPFADSSALPTYHVSRLARQSVTVA
HHHHHHHHHHHHCCHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEEE
LAGDGGDESFAGYQKYAIELKEDLARRAVPRALLSLIGKAAGAASHPLLRKVRSLTTSAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MEPAAAYYRTNTFVEDDLLAELLSEPFRRACAGYDPAEQTTRYFSRMQGADHVTAMLYTD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
LKSYLPGDILVKVDRMSMAHSLEVRAPFLDYRIVEFAARLPSSWKISGGEKKIILRKAFG
HHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHHH
RLLPDSVLNRPKQGFTVPLDTWFKKELREFAGSRLLDDPTLSQYLSLNVVRRVWEEHQQD
HCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RARHGTLLWSLLMLALWHQEYQGGAPCSK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCGIAGFFRTLCPEGDTRLLRRMGEVISHRGPDASGEFLDEEVGLSHRRLSILDLSPLGN
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC
QPMHSLDGRYVIVFNGEIYNFLELRGELEREGVSFRSRTDTEVILALYAREGVACLKRLN
CCHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GMFAFALWDRDTKTLLLARDRIGKKPLYYYHAGGDRIAFASEIKSLLEVPGVERQVEPTA
CCEEEEEECCCCCEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHH
VADYLKYLYIPAPKTIYRRLYKLPPAHCLELKAGEEPRIREYWDVEFKADYGLTPEAAEE
HHHHHHHHCCCCCHHHHHHHHHCCCHHEEECCCCCCCCCHHHCCEEEECCCCCCCCHHHH
ELLDLLQKATACRMIADVPLGAFLSGGVDSSAVVALMARAAQEPVKTCSIGFDDKRHDET
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PYAREVARLFATEHKEYRVSADLAGTVALLPRFFDEPFADSSALPTYHVSRLARQSVTVA
HHHHHHHHHHHHCCHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCEEEE
LAGDGGDESFAGYQKYAIELKEDLARRAVPRALLSLIGKAAGAASHPLLRKVRSLTTSAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
MEPAAAYYRTNTFVEDDLLAELLSEPFRRACAGYDPAEQTTRYFSRMQGADHVTAMLYTD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
LKSYLPGDILVKVDRMSMAHSLEVRAPFLDYRIVEFAARLPSSWKISGGEKKIILRKAFG
HHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEHHHHHHH
RLLPDSVLNRPKQGFTVPLDTWFKKELREFAGSRLLDDPTLSQYLSLNVVRRVWEEHQQD
HCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RARHGTLLWSLLMLALWHQEYQGGAPCSK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]