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The map label for this gene is wcaJ [H]

Identifier: 197118153

GI number: 197118153

Start: 2038679

End: 2040058

Strand: Direct

Name: wcaJ [H]

Synonym: Gbem_1768

Alternate gene names: 197118153

Gene position: 2038679-2040058 (Clockwise)

Preceding gene: 197118152

Following gene: 197118154

Centisome position: 44.17

GC content: 59.42

Gene sequence:

>1380_bases
ATGAACCTCCGTGTAGCCTTCAACCTTTTCACCGATGCCCTCATGGCTCTGGCCGCACTGGTTTTTGCGGCCGGCGTCCG
TTTCGGCCGCTTCGACCTGCCCGAAAACTGGGAGGCGGAGCAGGGGGTTGCTGCCGGCACGATCTTCGTGGGGGTCACCC
TTTTTTCCTCCTACCTCATGGAGGTGCACAGTCTCCCCAAGGAGAGCCGCAAACGCGAGATCTTTGCGGCCTGTTTGCAG
TCCGGCTGCGCCGCCTTTTTCCTGCTCTCCGTGCTCTACTATCTGGACCCGAGCCTCATGCTGGGGCGTGGCGTCCTTTT
CTTTGCCCTTGGCTTTTTCATCATCTTCCAGTTCGCGTGGTACGCCATTTCCGGAATTGAAGCCCGCACCGTTCCCTTCG
CGCAGCGCGTGCTGATCCTTGGCACTGGCGACCTTGCCTGCCAGCTGGGAGGACTGATCACCTCGCAGGGAGGTTCCTTC
ACCCTGGCCGGCTACCTTGAATGCGGTGAAGGGATGCCTGCGGCAACGGTGCACCAGTCCGAAACCTTCAATTCCCAGGT
GATGCCCGTTGAGGGGGACCTGTTGCAGACCGCGCGCAACCACAGGGTCTCCATCATCGTGGTGGCGCTTGCCGAGAGGA
GGGGAGTCCTGCCGTTGCAGGAGATGATGCGCTGCAAGCTGAACGGTATAGAGATCGTCGACGCTCCCACCTTTTACGAG
ATCGTACAGGGAAAGCTCATGCTTGAAGAGATGACGCCGAGCTGGATCATCTTTTCCTCCGGGTTTCGCAGGAACGCACT
CATCAACGTCTACAAGCGTTGCGTCGACATCCTCCTTTCAGTCGTCGGGCTCCTGCTGGCCGCCCCCCTTTTCCCCTTAA
TCGCCCTGGCCATCAAGCTGGATTCCCCGGGGCCGCTCTTCTTTAAACAGGTGCGCGTCGGCACCGGCGAAAAGCCGTTT
CTTTTGTACAAGTTCCGCTCCATGTGCCAGGACGCCGAGCGAAACGGCGCCGTCTGGGCAGCGAAAAACGACGCGCGGGT
CACCCGCGTAGGAAAGTTTCTGCGCAACTCGCGTATCGACGAGATTCCCCAGCTCTACAACGTGCTCAAGGGGGAGATGA
GCTTCATAGGCCCCAGGCCTGAGCGCCCCGAGTTCGTGGAGAACCTCAAGAAGGATATTTACTACTACTCGAAACGGCAC
ACCATCAAACCCGGGCTCACCGGGTGGGCCCAGGTCCGCTACCCCTACGGCGCCACGGTTGAGGACGCCAAGGAAAAGCT
CCGCTACGATCTCTACTACATCAAGAACCTCTCCTTCCTTCTTGACACGCAGATCATCTTCGAGACAGTGAAGGTGGTTT
TGTTTGGCCGGGGGCGGTAG

Upstream 100 bases:

>100_bases
CTGTTGCGCCGAGTTTATGGCTACGACCGGCAGCGATGCCGTCATACAGTTTCCTGTTCTTGTAGAAGGCCACTATAACC
GGCAGACCGGGAATAAGAGT

Downstream 100 bases:

>100_bases
GAGGGGAGGAGGTGCCCAGATGAAACTGAAAGTCCTGCTATTGTCCGTCTTTCTCATCACGTGCATGCCCCTTTTTGCCC
TGGCGGCTGACTACCAGATC

Product: glycosyltransferase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNLRVAFNLFTDALMALAALVFAAGVRFGRFDLPENWEAEQGVAAGTIFVGVTLFSSYLMEVHSLPKESRKREIFAACLQ
SGCAAFFLLSVLYYLDPSLMLGRGVLFFALGFFIIFQFAWYAISGIEARTVPFAQRVLILGTGDLACQLGGLITSQGGSF
TLAGYLECGEGMPAATVHQSETFNSQVMPVEGDLLQTARNHRVSIIVVALAERRGVLPLQEMMRCKLNGIEIVDAPTFYE
IVQGKLMLEEMTPSWIIFSSGFRRNALINVYKRCVDILLSVVGLLLAAPLFPLIALAIKLDSPGPLFFKQVRVGTGEKPF
LLYKFRSMCQDAERNGAVWAAKNDARVTRVGKFLRNSRIDEIPQLYNVLKGEMSFIGPRPERPEFVENLKKDIYYYSKRH
TIKPGLTGWAQVRYPYGATVEDAKEKLRYDLYYIKNLSFLLDTQIIFETVKVVLFGRGR

Sequences:

>Translated_459_residues
MNLRVAFNLFTDALMALAALVFAAGVRFGRFDLPENWEAEQGVAAGTIFVGVTLFSSYLMEVHSLPKESRKREIFAACLQ
SGCAAFFLLSVLYYLDPSLMLGRGVLFFALGFFIIFQFAWYAISGIEARTVPFAQRVLILGTGDLACQLGGLITSQGGSF
TLAGYLECGEGMPAATVHQSETFNSQVMPVEGDLLQTARNHRVSIIVVALAERRGVLPLQEMMRCKLNGIEIVDAPTFYE
IVQGKLMLEEMTPSWIIFSSGFRRNALINVYKRCVDILLSVVGLLLAAPLFPLIALAIKLDSPGPLFFKQVRVGTGEKPF
LLYKFRSMCQDAERNGAVWAAKNDARVTRVGKFLRNSRIDEIPQLYNVLKGEMSFIGPRPERPEFVENLKKDIYYYSKRH
TIKPGLTGWAQVRYPYGATVEDAKEKLRYDLYYIKNLSFLLDTQIIFETVKVVLFGRGR
>Mature_459_residues
MNLRVAFNLFTDALMALAALVFAAGVRFGRFDLPENWEAEQGVAAGTIFVGVTLFSSYLMEVHSLPKESRKREIFAACLQ
SGCAAFFLLSVLYYLDPSLMLGRGVLFFALGFFIIFQFAWYAISGIEARTVPFAQRVLILGTGDLACQLGGLITSQGGSF
TLAGYLECGEGMPAATVHQSETFNSQVMPVEGDLLQTARNHRVSIIVVALAERRGVLPLQEMMRCKLNGIEIVDAPTFYE
IVQGKLMLEEMTPSWIIFSSGFRRNALINVYKRCVDILLSVVGLLLAAPLFPLIALAIKLDSPGPLFFKQVRVGTGEKPF
LLYKFRSMCQDAERNGAVWAAKNDARVTRVGKFLRNSRIDEIPQLYNVLKGEMSFIGPRPERPEFVENLKKDIYYYSKRH
TIKPGLTGWAQVRYPYGATVEDAKEKLRYDLYYIKNLSFLLDTQIIFETVKVVLFGRGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=181, Percent_Identity=44.7513812154696, Blast_Score=158, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51429; Mature: 51429

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRVAFNLFTDALMALAALVFAAGVRFGRFDLPENWEAEQGVAAGTIFVGVTLFSSYLM
CCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVHSLPKESRKREIFAACLQSGCAAFFLLSVLYYLDPSLMLGRGVLFFALGFFIIFQFAW
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
YAISGIEARTVPFAQRVLILGTGDLACQLGGLITSQGGSFTLAGYLECGEGMPAATVHQS
HHHCCCCCCCCCHHCEEEEEECCHHHHHHCCEEECCCCCEEEEEEEECCCCCCCCEEECC
ETFNSQVMPVEGDLLQTARNHRVSIIVVALAERRGVLPLQEMMRCKLNGIEIVDAPTFYE
CCCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCEEEECCCHHHH
IVQGKLMLEEMTPSWIIFSSGFRRNALINVYKRCVDILLSVVGLLLAAPLFPLIALAIKL
HHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
DSPGPLFFKQVRVGTGEKPFLLYKFRSMCQDAERNGAVWAAKNDARVTRVGKFLRNSRID
CCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHH
EIPQLYNVLKGEMSFIGPRPERPEFVENLKKDIYYYSKRHTIKPGLTGWAQVRYPYGATV
HHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCH
EDAKEKLRYDLYYIKNLSFLLDTQIIFETVKVVLFGRGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLRVAFNLFTDALMALAALVFAAGVRFGRFDLPENWEAEQGVAAGTIFVGVTLFSSYLM
CCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVHSLPKESRKREIFAACLQSGCAAFFLLSVLYYLDPSLMLGRGVLFFALGFFIIFQFAW
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
YAISGIEARTVPFAQRVLILGTGDLACQLGGLITSQGGSFTLAGYLECGEGMPAATVHQS
HHHCCCCCCCCCHHCEEEEEECCHHHHHHCCEEECCCCCEEEEEEEECCCCCCCCEEECC
ETFNSQVMPVEGDLLQTARNHRVSIIVVALAERRGVLPLQEMMRCKLNGIEIVDAPTFYE
CCCCCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCEEEECCCHHHH
IVQGKLMLEEMTPSWIIFSSGFRRNALINVYKRCVDILLSVVGLLLAAPLFPLIALAIKL
HHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
DSPGPLFFKQVRVGTGEKPFLLYKFRSMCQDAERNGAVWAAKNDARVTRVGKFLRNSRID
CCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHH
EIPQLYNVLKGEMSFIGPRPERPEFVENLKKDIYYYSKRHTIKPGLTGWAQVRYPYGATV
HHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCH
EDAKEKLRYDLYYIKNLSFLLDTQIIFETVKVVLFGRGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]