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The map label for this gene is cysW [H]

Identifier: 197118123

GI number: 197118123

Start: 2002468

End: 2003349

Strand: Direct

Name: cysW [H]

Synonym: Gbem_1738

Alternate gene names: 197118123

Gene position: 2002468-2003349 (Clockwise)

Preceding gene: 197118122

Following gene: 197118124

Centisome position: 43.39

GC content: 61.45

Gene sequence:

>882_bases
ATGCAGGTAGGCAGGGTATCGAAAAAGGAAAAACAAAGAGGAGGGGGGAGCACCTCGGAACCCGCGTGGGTGCGCTACCT
GCTGGTGACGGTGGCGCTTATATTCCTGGCTCTCTTCCTGCTGCTCCCTTTGGCGGCGGTCTTCGTGCAGGCGCTGGAAA
AGGGGTGGGAGGCCTACTTCGAAGCGCTCAAGGAACCGGATACGCTGTCGGCCATCCGCCTCACCCTGACTACGGCCGCG
GTCTGCGTCCCTCTGAACCTCTTCTTCGGGATCGTCGCTTCCTGGGCCATCGCCAAGTTCAACTTCCGCGGCAAGAGCTT
CCTGATCACGCTGATAGACCTGCCGTTCTCGGTTTCCCCGGTGGTCTCGGGTCTCATCTATGTGCTCATCTTCGGGCTGC
AAGGGTGGCTCGGCCCCTGGCTGCAGGACCACAACATCAAGATTATCTTCGCGGTCCCGGGGATTGTCCTCGCCACCGTC
TTCGTCACCTTCCCGTTCGTCGCGCGTGAACTGATCCCTCTCATGGAGGCGCAGGGGCGCGAGGAGGAAGAGGCGGCGCT
GACGCTTGGGGCTAGCGGGCTGCAGACCTTTTTCCGGGTCACCTTCCCCAACATCAAGTGGGGCATCCTCTACGGCGTCA
TCCTCTGCAACGCGAGGGCGATGGGGGAATTCGGCGCCGTCTCGGTCGTGTCGGGGCATGTGCGCGGCTCGACCAATACC
ATCCCCCTCCAGGTGGAGATCCTCTACAACGAATACAGTTACGCGGCGGCCTTTGCCGTCGCCTCGCTCCTTGCGCTGCT
GGCGCTGGTGACCCTAGCCGTAAAGACTGCGGCCGAGTGGAAAATGCAACAGACCGTAACGGTCTCGGAAAAAGAAAGGT
AG

Upstream 100 bases:

>100_bases
TGCTCGTGGTGTCGTTTCTGATGCTTCTTTGCATCAATCTTTTGCAGAAGTGGAGCAGGCGTTTCGCGGATTAGGGGCGT
AGCCTCGGGAGGGACGGAAG

Downstream 100 bases:

>100_bases
TCCCATGAGCATCGATATCGAGGCGGTCAGCAAGACATTCGGCAGCTTTAGCGCACTGAGCAACGTGAGCCTTAAGGTCC
CCTCCGGGGAACTGGTGGCG

Product: sulfate ABC transporter membrane protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MQVGRVSKKEKQRGGGSTSEPAWVRYLLVTVALIFLALFLLLPLAAVFVQALEKGWEAYFEALKEPDTLSAIRLTLTTAA
VCVPLNLFFGIVASWAIAKFNFRGKSFLITLIDLPFSVSPVVSGLIYVLIFGLQGWLGPWLQDHNIKIIFAVPGIVLATV
FVTFPFVARELIPLMEAQGREEEEAALTLGASGLQTFFRVTFPNIKWGILYGVILCNARAMGEFGAVSVVSGHVRGSTNT
IPLQVEILYNEYSYAAAFAVASLLALLALVTLAVKTAAEWKMQQTVTVSEKER

Sequences:

>Translated_293_residues
MQVGRVSKKEKQRGGGSTSEPAWVRYLLVTVALIFLALFLLLPLAAVFVQALEKGWEAYFEALKEPDTLSAIRLTLTTAA
VCVPLNLFFGIVASWAIAKFNFRGKSFLITLIDLPFSVSPVVSGLIYVLIFGLQGWLGPWLQDHNIKIIFAVPGIVLATV
FVTFPFVARELIPLMEAQGREEEEAALTLGASGLQTFFRVTFPNIKWGILYGVILCNARAMGEFGAVSVVSGHVRGSTNT
IPLQVEILYNEYSYAAAFAVASLLALLALVTLAVKTAAEWKMQQTVTVSEKER
>Mature_293_residues
MQVGRVSKKEKQRGGGSTSEPAWVRYLLVTVALIFLALFLLLPLAAVFVQALEKGWEAYFEALKEPDTLSAIRLTLTTAA
VCVPLNLFFGIVASWAIAKFNFRGKSFLITLIDLPFSVSPVVSGLIYVLIFGLQGWLGPWLQDHNIKIIFAVPGIVLATV
FVTFPFVARELIPLMEAQGREEEEAALTLGASGLQTFFRVTFPNIKWGILYGVILCNARAMGEFGAVSVVSGHVRGSTNT
IPLQVEILYNEYSYAAAFAVASLLALLALVTLAVKTAAEWKMQQTVTVSEKER

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=271, Percent_Identity=49.4464944649446, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1788764, Length=216, Percent_Identity=35.1851851851852, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1786980, Length=220, Percent_Identity=31.8181818181818, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787369, Length=185, Percent_Identity=28.6486486486486, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32048; Mature: 32048

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVGRVSKKEKQRGGGSTSEPAWVRYLLVTVALIFLALFLLLPLAAVFVQALEKGWEAYF
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALKEPDTLSAIRLTLTTAAVCVPLNLFFGIVASWAIAKFNFRGKSFLITLIDLPFSVSP
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHH
VVSGLIYVLIFGLQGWLGPWLQDHNIKIIFAVPGIVLATVFVTFPFVARELIPLMEAQGR
HHHHHHHHHHHHHHHHHCCCEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEEEAALTLGASGLQTFFRVTFPNIKWGILYGVILCNARAMGEFGAVSVVSGHVRGSTNT
CHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHEEECCCCCCCCE
IPLQVEILYNEYSYAAAFAVASLLALLALVTLAVKTAAEWKMQQTVTVSEKER
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MQVGRVSKKEKQRGGGSTSEPAWVRYLLVTVALIFLALFLLLPLAAVFVQALEKGWEAYF
CCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALKEPDTLSAIRLTLTTAAVCVPLNLFFGIVASWAIAKFNFRGKSFLITLIDLPFSVSP
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHH
VVSGLIYVLIFGLQGWLGPWLQDHNIKIIFAVPGIVLATVFVTFPFVARELIPLMEAQGR
HHHHHHHHHHHHHHHHHCCCEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEEEAALTLGASGLQTFFRVTFPNIKWGILYGVILCNARAMGEFGAVSVVSGHVRGSTNT
CHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCCCCHHEEECCCCCCCCE
IPLQVEILYNEYSYAAAFAVASLLALLALVTLAVKTAAEWKMQQTVTVSEKER
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]