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The map label for this gene is cysU [H]

Identifier: 197118122

GI number: 197118122

Start: 2001611

End: 2002441

Strand: Direct

Name: cysU [H]

Synonym: Gbem_1737

Alternate gene names: 197118122

Gene position: 2001611-2002441 (Clockwise)

Preceding gene: 197118117

Following gene: 197118123

Centisome position: 43.37

GC content: 61.37

Gene sequence:

>831_bases
ATGTCCTGGTCAAGCGGGAAAAATAACGTACTACCCGGTTTCACCCCGGCACTCGGGTATACCGTCTTCTACCTGAGCCT
CATCGTCCTCATCCCCCTTTCCGCACTCTTCTTCAAGACCTCGACCCTTTCCTGGGACGACTTCGTCACTGCCGTGAGCG
CGCCTCGGGTGCTTGCTTCCTACCGCGTCACCTTCGGGGCTTCGCTCGCCGCTGCTGTCGTCAACGGCTTCTTCGGCGTT
CTGGTCGCCTGGGTGCTGGTGCGCTACCGCTTCCCCGGCAAGAAGCTCATCGACGCGCTGGTCGATCTTCCCTTCGCCCT
CCCCACGGCGGTAGCAGGCATCACCCTGGCAAGCATCTATTCCTCGAACGGCTGGCTCGGGCGCTACCTGGAGCCGCGCG
GGATCAAGATCGCCTTCACTCCTCTGGGCATCATCATCGCCATGATCTTCATCGGCCTTCCTTTCGTGGTCCGCACGGTG
CAGCCGGTCCTGGAAGAGCTGGACCACGAAATCGAGGAAGCCGCCTCCTGTCTGGGAGCCGGCCGCTGGCAGACCTTCCG
CAGGGTGCTGCTGCCGACCATGCTTCCGGCCATACTGACCGGGTTCGCACTGGCGTTCGCCCGGGCAGTGGGGGAGTACG
GCTCCATCATCTTCATCGCGGGGAACATGCCGATGGTCTCCGAGATAACGCCGCTGCTCATCATCACCAAGCTGGAGCAG
TACGATTACGCGGGCGCTACCGCCATCGCGACCGTAATGCTCGTGGTGTCGTTTCTGATGCTTCTTTGCATCAATCTTTT
GCAGAAGTGGAGCAGGCGTTTCGCGGATTAG

Upstream 100 bases:

>100_bases
CTTGACAAAAGGACTGCGGTGGTGGTAGGTTTTGTCCACTAATTCGATGGGGTAACCATTTCGACCATCGGCTTTAGCTG
CAAAGAACGGAGGAGGGGAC

Downstream 100 bases:

>100_bases
GGGCGTAGCCTCGGGAGGGACGGAAGATGCAGGTAGGCAGGGTATCGAAAAAGGAAAAACAAAGAGGAGGGGGGAGCACC
TCGGAACCCGCGTGGGTGCG

Product: sulfate ABC transporter membrane protein CysU

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSWSSGKNNVLPGFTPALGYTVFYLSLIVLIPLSALFFKTSTLSWDDFVTAVSAPRVLASYRVTFGASLAAAVVNGFFGV
LVAWVLVRYRFPGKKLIDALVDLPFALPTAVAGITLASIYSSNGWLGRYLEPRGIKIAFTPLGIIIAMIFIGLPFVVRTV
QPVLEELDHEIEEAASCLGAGRWQTFRRVLLPTMLPAILTGFALAFARAVGEYGSIIFIAGNMPMVSEITPLLIITKLEQ
YDYAGATAIATVMLVVSFLMLLCINLLQKWSRRFAD

Sequences:

>Translated_276_residues
MSWSSGKNNVLPGFTPALGYTVFYLSLIVLIPLSALFFKTSTLSWDDFVTAVSAPRVLASYRVTFGASLAAAVVNGFFGV
LVAWVLVRYRFPGKKLIDALVDLPFALPTAVAGITLASIYSSNGWLGRYLEPRGIKIAFTPLGIIIAMIFIGLPFVVRTV
QPVLEELDHEIEEAASCLGAGRWQTFRRVLLPTMLPAILTGFALAFARAVGEYGSIIFIAGNMPMVSEITPLLIITKLEQ
YDYAGATAIATVMLVVSFLMLLCINLLQKWSRRFAD
>Mature_275_residues
SWSSGKNNVLPGFTPALGYTVFYLSLIVLIPLSALFFKTSTLSWDDFVTAVSAPRVLASYRVTFGASLAAAVVNGFFGVL
VAWVLVRYRFPGKKLIDALVDLPFALPTAVAGITLASIYSSNGWLGRYLEPRGIKIAFTPLGIIIAMIFIGLPFVVRTVQ
PVLEELDHEIEEAASCLGAGRWQTFRRVLLPTMLPAILTGFALAFARAVGEYGSIIFIAGNMPMVSEITPLLIITKLEQY
DYAGATAIATVMLVVSFLMLLCINLLQKWSRRFAD

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=268, Percent_Identity=52.2388059701493, Blast_Score=258, Evalue=2e-70,
Organism=Escherichia coli, GI87082099, Length=251, Percent_Identity=33.0677290836653, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786980, Length=200, Percent_Identity=36, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1786254, Length=225, Percent_Identity=27.5555555555556, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787369, Length=162, Percent_Identity=32.0987654320988, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787368, Length=232, Percent_Identity=26.7241379310345, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30110; Mature: 29979

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWSSGKNNVLPGFTPALGYTVFYLSLIVLIPLSALFFKTSTLSWDDFVTAVSAPRVLAS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YRVTFGASLAAAVVNGFFGVLVAWVLVRYRFPGKKLIDALVDLPFALPTAVAGITLASIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
SSNGWLGRYLEPRGIKIAFTPLGIIIAMIFIGLPFVVRTVQPVLEELDHEIEEAASCLGA
CCCCCEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRWQTFRRVLLPTMLPAILTGFALAFARAVGEYGSIIFIAGNMPMVSEITPLLIITKLEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
YDYAGATAIATVMLVVSFLMLLCINLLQKWSRRFAD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SWSSGKNNVLPGFTPALGYTVFYLSLIVLIPLSALFFKTSTLSWDDFVTAVSAPRVLAS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YRVTFGASLAAAVVNGFFGVLVAWVLVRYRFPGKKLIDALVDLPFALPTAVAGITLASIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
SSNGWLGRYLEPRGIKIAFTPLGIIIAMIFIGLPFVVRTVQPVLEELDHEIEEAASCLGA
CCCCCEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRWQTFRRVLLPTMLPAILTGFALAFARAVGEYGSIIFIAGNMPMVSEITPLLIITKLEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
YDYAGATAIATVMLVVSFLMLLCINLLQKWSRRFAD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]