Click here to switch to the map view.

The map label for this gene is metK-3 [H]

Identifier: 197118110

GI number: 197118110

Start: 1988949

End: 1990157

Strand: Direct

Name: metK-3 [H]

Synonym: Gbem_1725

Alternate gene names: 197118110

Gene position: 1988949-1990157 (Clockwise)

Preceding gene: 197118109

Following gene: 197118111

Centisome position: 43.1

GC content: 66.09

Gene sequence:

>1209_bases
ATGTCCGAGCAGCGCTTTTTATTCACCTCCGAATCCGTCACCGAGGGGCATCCCGACAAGCTGGCGGACCAGATTTCCGA
TGCCGTTCTCGATGCCCTGCTGGCGCAGGATCCCGACTCCCGCGTGGCTTGCGAGACCAGCGTGACTACCGGACTCGTGC
TCCTCTTCGGCGAGGTCTCCAGCCAGGCGCGCGTCAACTTCGCCCGCATCGCCCGCGACACCATCGCCCAGGTCGGCTAC
ACCGACACGAAATTCGGGATAGACGCGGAGCACGCCGCGGTGCTGGTCTCTCTCGACCAGCAGTCCGCCGACATCGCCCA
AGGGGTCGACGTGGCGCTGGAGCAGCGCAAGGGTAGCGATCACGACGGCTACCTCGACTCGGTAGGTGCCGGGGACCAGG
GGATGGTCTTCGGCTTCGCCACCGACGAGACGCCGGAGTACCTTCCCACCAGCATCGCCCTGGCGCACAAGCTCGCGCGC
CGGCTCACCGAGGCCCGCAAGTCCGGCGAAATCCCTTATCTCCGTCCGGACGGAAAGACACAGGTGACGGTCGAGTATCG
GGGGGACACCCCGGTACGGGTCGACACGGTGGTCATCTCCGCCCAGCACGACCCCGAGGTAGAGATCGAGCAGATCCGCG
AGGACCTTGTCGCCAAGGTGATCGAAAAGGTCGTGCCCGAAGGACTCCTGGACGACAAGACCCGCATCTTCGTGAACCCT
ACCGGCCGTTTCGTCATCGGCGGACCGCACGGAGACGCCGGGCTCACCGGCAGGAAGATCATCGTGGACAGCTACGGCGG
TTTCTCCCGCCACGGAGGGGGAGCCTTCTCCGGGAAGGACTCCACCAAGGTCGACCGCTCCGGCGCCTATGCCGCCCGCT
ACGTCGCCAAGAACCTGGTCGCCGCCGGGCTCGCCAAGAAGGTGGAGGTGCAGATCGCCTACGCCATCGGCGTCGCCCGC
CCGGTTTCCATCTTCGTCGACAGCTTTGGGACCGGCGTCCTCCGCAACGAGGAACTCGCGGGGCTCATCTCCAGCCATTT
CGACCTGAGGCCGGCGGCGATCATAGAAGAGCTGGGGCTCAAGCGCCCCATCTACCGCCAGACCGCAGCCTACGGCCATT
TCGGGAGGCCCGATCTCGATCTTCCGTGGGAGCGGACCGACAAGGCGCAAGCCTTGCGCGATGCGGCTCGCGGCGCGGCA
ACCAACTGA

Upstream 100 bases:

>100_bases
GAGCTGACCAGACAAACTGGAGGCCAGGCTGTAACCGTTTACCTACGGTTATGCCTGGCCTCTTTTTTTTCCGGCAGCCG
AGACAAAAGGAGAAGCGACC

Downstream 100 bases:

>100_bases
TAGAGGTTCCAGAAGGAGGGACATCATGGCAACAGCGATACCCGGAGACCTGATCGAGCTACTGAACGATCCCGCCGCGA
CCAAGGTGCTGGCCACCGTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSEQRFLFTSESVTEGHPDKLADQISDAVLDALLAQDPDSRVACETSVTTGLVLLFGEVSSQARVNFARIARDTIAQVGY
TDTKFGIDAEHAAVLVSLDQQSADIAQGVDVALEQRKGSDHDGYLDSVGAGDQGMVFGFATDETPEYLPTSIALAHKLAR
RLTEARKSGEIPYLRPDGKTQVTVEYRGDTPVRVDTVVISAQHDPEVEIEQIREDLVAKVIEKVVPEGLLDDKTRIFVNP
TGRFVIGGPHGDAGLTGRKIIVDSYGGFSRHGGGAFSGKDSTKVDRSGAYAARYVAKNLVAAGLAKKVEVQIAYAIGVAR
PVSIFVDSFGTGVLRNEELAGLISSHFDLRPAAIIEELGLKRPIYRQTAAYGHFGRPDLDLPWERTDKAQALRDAARGAA
TN

Sequences:

>Translated_402_residues
MSEQRFLFTSESVTEGHPDKLADQISDAVLDALLAQDPDSRVACETSVTTGLVLLFGEVSSQARVNFARIARDTIAQVGY
TDTKFGIDAEHAAVLVSLDQQSADIAQGVDVALEQRKGSDHDGYLDSVGAGDQGMVFGFATDETPEYLPTSIALAHKLAR
RLTEARKSGEIPYLRPDGKTQVTVEYRGDTPVRVDTVVISAQHDPEVEIEQIREDLVAKVIEKVVPEGLLDDKTRIFVNP
TGRFVIGGPHGDAGLTGRKIIVDSYGGFSRHGGGAFSGKDSTKVDRSGAYAARYVAKNLVAAGLAKKVEVQIAYAIGVAR
PVSIFVDSFGTGVLRNEELAGLISSHFDLRPAAIIEELGLKRPIYRQTAAYGHFGRPDLDLPWERTDKAQALRDAARGAA
TN
>Mature_401_residues
SEQRFLFTSESVTEGHPDKLADQISDAVLDALLAQDPDSRVACETSVTTGLVLLFGEVSSQARVNFARIARDTIAQVGYT
DTKFGIDAEHAAVLVSLDQQSADIAQGVDVALEQRKGSDHDGYLDSVGAGDQGMVFGFATDETPEYLPTSIALAHKLARR
LTEARKSGEIPYLRPDGKTQVTVEYRGDTPVRVDTVVISAQHDPEVEIEQIREDLVAKVIEKVVPEGLLDDKTRIFVNPT
GRFVIGGPHGDAGLTGRKIIVDSYGGFSRHGGGAFSGKDSTKVDRSGAYAARYVAKNLVAAGLAKKVEVQIAYAIGVARP
VSIFVDSFGTGVLRNEELAGLISSHFDLRPAAIIEELGLKRPIYRQTAAYGHFGRPDLDLPWERTDKAQALRDAARGAAT
N

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=56.1855670103093, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI5174529, Length=394, Percent_Identity=55.3299492385787, Blast_Score=420, Evalue=1e-117,
Organism=Escherichia coli, GI1789311, Length=394, Percent_Identity=56.0913705583756, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=56.2982005141388, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17538494, Length=394, Percent_Identity=55.5837563451777, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25145633, Length=407, Percent_Identity=54.2997542997543, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=401, Percent_Identity=53.8653366583541, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=394, Percent_Identity=51.7766497461929, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=53.8461538461538, Blast_Score=354, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=356, Percent_Identity=51.4044943820225, Blast_Score=347, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=316, Percent_Identity=54.746835443038, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=57.4712643678161, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=77.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323209, Length=384, Percent_Identity=56.7708333333333, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320710, Length=395, Percent_Identity=54.9367088607595, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24580527, Length=383, Percent_Identity=51.9582245430809, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=383, Percent_Identity=51.9582245430809, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=383, Percent_Identity=52.2193211488251, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=383, Percent_Identity=52.2193211488251, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=383, Percent_Identity=52.2193211488251, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=383, Percent_Identity=52.2193211488251, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=383, Percent_Identity=52.2193211488251, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=383, Percent_Identity=52.2193211488251, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=50.5405405405405, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580539, Length=128, Percent_Identity=50, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43278; Mature: 43147

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRFLFTSESVTEGHPDKLADQISDAVLDALLAQDPDSRVACETSVTTGLVLLFGEVS
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCEEEEEECCC
SQARVNFARIARDTIAQVGYTDTKFGIDAEHAAVLVSLDQQSADIAQGVDVALEQRKGSD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCC
HDGYLDSVGAGDQGMVFGFATDETPEYLPTSIALAHKLARRLTEARKSGEIPYLRPDGKT
CCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCE
QVTVEYRGDTPVRVDTVVISAQHDPEVEIEQIREDLVAKVIEKVVPEGLLDDKTRIFVNP
EEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
TGRFVIGGPHGDAGLTGRKIIVDSYGGFSRHGGGAFSGKDSTKVDRSGAYAARYVAKNLV
CCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AAGLAKKVEVQIAYAIGVARPVSIFVDSFGTGVLRNEELAGLISSHFDLRPAAIIEELGL
HHHCCHHEEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC
KRPIYRQTAAYGHFGRPDLDLPWERTDKAQALRDAARGAATN
CCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEQRFLFTSESVTEGHPDKLADQISDAVLDALLAQDPDSRVACETSVTTGLVLLFGEVS
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCEEEEEECCC
SQARVNFARIARDTIAQVGYTDTKFGIDAEHAAVLVSLDQQSADIAQGVDVALEQRKGSD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCC
HDGYLDSVGAGDQGMVFGFATDETPEYLPTSIALAHKLARRLTEARKSGEIPYLRPDGKT
CCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCE
QVTVEYRGDTPVRVDTVVISAQHDPEVEIEQIREDLVAKVIEKVVPEGLLDDKTRIFVNP
EEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
TGRFVIGGPHGDAGLTGRKIIVDSYGGFSRHGGGAFSGKDSTKVDRSGAYAARYVAKNLV
CCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AAGLAKKVEVQIAYAIGVARPVSIFVDSFGTGVLRNEELAGLISSHFDLRPAAIIEELGL
HHHCCHHEEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCC
KRPIYRQTAAYGHFGRPDLDLPWERTDKAQALRDAARGAATN
CCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA