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The map label for this gene is fumB [H]

Identifier: 197117279

GI number: 197117279

Start: 1041097

End: 1042722

Strand: Reverse

Name: fumB [H]

Synonym: Gbem_0889

Alternate gene names: 197117279

Gene position: 1042722-1041097 (Counterclockwise)

Preceding gene: 197117280

Following gene: 197117275

Centisome position: 22.59

GC content: 63.84

Gene sequence:

>1626_bases
ATGGCAACTCCAGAGTTCAAGTACCAGGAAGCCTTCCCGCTCGGACCGGACAGCACCAAGTACCGCCTGATCCCGGATTC
CAAGCAGTACGTGTCGGTGTCCAACTTCGAGGGGCAGGAGATCCTCAAGGTGGCGCCCGAGGCGCTTACCATCGCGGCCA
ACGCCGCCATGAAGGACCTCTCCTTCATGCTGCGCCCGGAGCACAATGAGCAGGTAGCCAAGATCCTCGCCGACCCGGAA
GCCTCCCAAAACGACAAGGGTGTCGCCCTCGCCTTCCTGAGAAACGCAGAGGTCGCAGCGCAGCTCGAACTCCCCATCTG
CCAGGACACCGGCACCGCCATCGTCATGGGCAAGAAAGGGCAGCAGGTCTGGACCGGCGCCAACGACGAGGAGTACCTCT
CCAAGGGGGTCTACAAGACCTACACCGAGGAGAACCTGCGCTACTCCCAGACCGTGGCTCTGGACATGTACAAGGAGATC
AACACCGGCACCAACCTCCCGGCCCAGATCGACGTCTACGCGACCAAAGGGGCCGAGTACAAGTTCCTTTTCATGGCCAA
GGGGGGCGGCTCCGCCAACAAGACCTATCTCTACCAGGAGACCAAGGCGCTCCTTAACCCGGACAGCCTGATCAAGTTTC
TGGTCGCCAAGATGAAGACCCTCGGGACCGCGGCCTGCCCGCCGTACCACTTGGCCTTCGCCATCGGCGGCACCTCCGCC
GAGGCGTGCCTGAAGACCGTGAAGCTCGCCAGCGCCAAATACCTGGACGCCCTCCCCACCTCCGGCAACGACGGTGGCCA
GGCGTTCCGCGACGTCGAGCTCGAGCAGCAGCTCCTCAAGGCGGCCCAGGAATCCGGCATCGGCGCGCAGTTCGGCGGCA
AGTACTTCGCCCACGACGTGCGCATCGTCCGCCTGCCCCGCCACGGCGCCTCCTGCCCGGTCGGCATGGGGGTCTCCTGC
TCCGCTGACCGCAACATCAAGGCGAAGATCAACAAGGAAGGGATCTGGGTCGAGCAGATGGACGACAACCCGGGCCGCCT
GATCCCCGAGCGTTTCCGCGGCAAGCACGAGCACGGCGTCAGGATCAACCTGAACCAGCCGCTCAAGGACGTCCTTGCCG
AGTTGACCAAGCACCCGGTCTCCACGCCGCTGCTCCTCTCCGGAACCATCGTGGTCGGCCGCGACATCGCCCACGCCAAG
TTCAAGGAGATACTCGACTCCGGCAAGCCGCTTCCCGAGTACCTGAAGAACCACCCGATCTACTACGCGGGCCCGGCGAA
GACCCCGAAAGGGAAGCCCTCCGGCTCCTTCGGCCCCACCACCGCCGGTCGCATGGACTCCTACGTCGACCTCCTGCAGG
CAAACGGCGGCTCCATGATCATGATCGCCAAGGGCAACCGCTCCCAGCAGGTCACCGACGCCTGTAAGAAGCACGGCGGC
TTCTACCTGGGCTCCATCGGCGGCCCGGCTGCCATCCTGGCCGAGGAGAACATCAAGAAGGTCGAGTGCATCGACTTCCC
CGAGCTCGGCATGGAGGCGGTTTGGAAGATAGAGGTTGAGGACTTCCCGGCGTTCATCCTGGTGGACGACAAGGGGAACG
ACTTCTTCAAGCAGCTGGGCATCTAG

Upstream 100 bases:

>100_bases
ATTCCGGGATACTGTATACAAATTTTTCCTGCCTTCAGTCCCGGCAGGAGCCGATAGAGTAGATTGTCACCATCATCCCA
GCAAAAGGAGAAGAGAATCG

Downstream 100 bases:

>100_bases
GCGCCTTCGCGCCTAGGGCTACGGCCCCCAAGCTGTAACAGCGAAACGGAAAAGCCCCCGTTGCCGTCAAGGCAGCGGGG
GCTTTTCCGTTGGAGGAGTA

Product: class I fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MATPEFKYQEAFPLGPDSTKYRLIPDSKQYVSVSNFEGQEILKVAPEALTIAANAAMKDLSFMLRPEHNEQVAKILADPE
ASQNDKGVALAFLRNAEVAAQLELPICQDTGTAIVMGKKGQQVWTGANDEEYLSKGVYKTYTEENLRYSQTVALDMYKEI
NTGTNLPAQIDVYATKGAEYKFLFMAKGGGSANKTYLYQETKALLNPDSLIKFLVAKMKTLGTAACPPYHLAFAIGGTSA
EACLKTVKLASAKYLDALPTSGNDGGQAFRDVELEQQLLKAAQESGIGAQFGGKYFAHDVRIVRLPRHGASCPVGMGVSC
SADRNIKAKINKEGIWVEQMDDNPGRLIPERFRGKHEHGVRINLNQPLKDVLAELTKHPVSTPLLLSGTIVVGRDIAHAK
FKEILDSGKPLPEYLKNHPIYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
FYLGSIGGPAAILAEENIKKVECIDFPELGMEAVWKIEVEDFPAFILVDDKGNDFFKQLGI

Sequences:

>Translated_541_residues
MATPEFKYQEAFPLGPDSTKYRLIPDSKQYVSVSNFEGQEILKVAPEALTIAANAAMKDLSFMLRPEHNEQVAKILADPE
ASQNDKGVALAFLRNAEVAAQLELPICQDTGTAIVMGKKGQQVWTGANDEEYLSKGVYKTYTEENLRYSQTVALDMYKEI
NTGTNLPAQIDVYATKGAEYKFLFMAKGGGSANKTYLYQETKALLNPDSLIKFLVAKMKTLGTAACPPYHLAFAIGGTSA
EACLKTVKLASAKYLDALPTSGNDGGQAFRDVELEQQLLKAAQESGIGAQFGGKYFAHDVRIVRLPRHGASCPVGMGVSC
SADRNIKAKINKEGIWVEQMDDNPGRLIPERFRGKHEHGVRINLNQPLKDVLAELTKHPVSTPLLLSGTIVVGRDIAHAK
FKEILDSGKPLPEYLKNHPIYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
FYLGSIGGPAAILAEENIKKVECIDFPELGMEAVWKIEVEDFPAFILVDDKGNDFFKQLGI
>Mature_540_residues
ATPEFKYQEAFPLGPDSTKYRLIPDSKQYVSVSNFEGQEILKVAPEALTIAANAAMKDLSFMLRPEHNEQVAKILADPEA
SQNDKGVALAFLRNAEVAAQLELPICQDTGTAIVMGKKGQQVWTGANDEEYLSKGVYKTYTEENLRYSQTVALDMYKEIN
TGTNLPAQIDVYATKGAEYKFLFMAKGGGSANKTYLYQETKALLNPDSLIKFLVAKMKTLGTAACPPYHLAFAIGGTSAE
ACLKTVKLASAKYLDALPTSGNDGGQAFRDVELEQQLLKAAQESGIGAQFGGKYFAHDVRIVRLPRHGASCPVGMGVSCS
ADRNIKAKINKEGIWVEQMDDNPGRLIPERFRGKHEHGVRINLNQPLKDVLAELTKHPVSTPLLLSGTIVVGRDIAHAKF
KEILDSGKPLPEYLKNHPIYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGGF
YLGSIGGPAAILAEENIKKVECIDFPELGMEAVWKIEVEDFPAFILVDDKGNDFFKQLGI

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=540, Percent_Identity=72.4074074074074, Blast_Score=834, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=540, Percent_Identity=72.2222222222222, Blast_Score=826, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=28.4916201117318, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 58891; Mature: 58760

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPEFKYQEAFPLGPDSTKYRLIPDSKQYVSVSNFEGQEILKVAPEALTIAANAAMKDL
CCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHCCCCEEEEHHHHHHHH
SFMLRPEHNEQVAKILADPEASQNDKGVALAFLRNAEVAAQLELPICQDTGTAIVMGKKG
HHEECCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEECCEECCCCCEEEEECCC
QQVWTGANDEEYLSKGVYKTYTEENLRYSQTVALDMYKEINTGTNLPAQIDVYATKGAEY
CEEECCCCHHHHHHCCCHHHHCCCCCCCHHEEHHHHHHHHCCCCCCCEEEEEEEECCCCE
KFLFMAKGGGSANKTYLYQETKALLNPDSLIKFLVAKMKTLGTAACPPYHLAFAIGGTSA
EEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
EACLKTVKLASAKYLDALPTSGNDGGQAFRDVELEQQLLKAAQESGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
RIVRLPRHGASCPVGMGVSCSADRNIKAKINKEGIWVEQMDDNPGRLIPERFRGKHEHGV
EEEEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEECCCCCCCCCCHHHCCCCCCCE
RINLNQPLKDVLAELTKHPVSTPLLLSGTIVVGRDIAHAKFKEILDSGKPLPEYLKNHPI
EEECCCCHHHHHHHHHCCCCCCCEEEECEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCE
YYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCC
FYLGSIGGPAAILAEENIKKVECIDFPELGMEAVWKIEVEDFPAFILVDDKGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
I
C
>Mature Secondary Structure 
ATPEFKYQEAFPLGPDSTKYRLIPDSKQYVSVSNFEGQEILKVAPEALTIAANAAMKDL
CCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCHHHHHHCCCCEEEEHHHHHHHH
SFMLRPEHNEQVAKILADPEASQNDKGVALAFLRNAEVAAQLELPICQDTGTAIVMGKKG
HHEECCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEECCEECCCCCEEEEECCC
QQVWTGANDEEYLSKGVYKTYTEENLRYSQTVALDMYKEINTGTNLPAQIDVYATKGAEY
CEEECCCCHHHHHHCCCHHHHCCCCCCCHHEEHHHHHHHHCCCCCCCEEEEEEEECCCCE
KFLFMAKGGGSANKTYLYQETKALLNPDSLIKFLVAKMKTLGTAACPPYHLAFAIGGTSA
EEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
EACLKTVKLASAKYLDALPTSGNDGGQAFRDVELEQQLLKAAQESGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
RIVRLPRHGASCPVGMGVSCSADRNIKAKINKEGIWVEQMDDNPGRLIPERFRGKHEHGV
EEEEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEECCCCCCCCCCHHHCCCCCCCE
RINLNQPLKDVLAELTKHPVSTPLLLSGTIVVGRDIAHAKFKEILDSGKPLPEYLKNHPI
EEECCCCHHHHHHHHHCCCCCCCEEEECEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCE
YYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCC
FYLGSIGGPAAILAEENIKKVECIDFPELGMEAVWKIEVEDFPAFILVDDKGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]