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The map label for this gene is kdtA [H]

Identifier: 197117239

GI number: 197117239

Start: 1001891

End: 1003192

Strand: Direct

Name: kdtA [H]

Synonym: Gbem_0847

Alternate gene names: 197117239

Gene position: 1001891-1003192 (Clockwise)

Preceding gene: 197117238

Following gene: 308535196

Centisome position: 21.71

GC content: 66.59

Gene sequence:

>1302_bases
ATGATCGACTTCATTTACAACCTGCTGCTCTGGCTGCTTTTGCCTTTGCTGGTCCCCTACCATGCCTACCGCTCCTTGAG
CCGGGGGAGGCGCACCGCCTTCATGGAGCGCTTCGGCGCCATCCCCGAAGCCGAGTTGGAGCCGCTCAAAGGCAAGAGGA
CCATCCTGGTCCATGCCGTCTCGGTGGGGGAGACCCTCGCCGCCCAACCGCTCCTCAAGGGGATCCGCTCGCGCTTCCCG
GAGCACCGGGTCGTGATCACCAACGTCACCGAGACCGGCCGCGGCGTCGCCCTCAAAAGCAACTCCGCCGATCTCTGCAT
CTATTTTCCCTTCGATTACCCCTTCGCCGTCCGCGCTGTCCTCAAGAAGGTGAGCCCCGACCTCGTCGTCATCATGGAGA
CCGAGATCTGGCCCAACTTCATCAAGGAGGCAGGGAGGCTCGGCATCCCGGTGCTCCTCGCCAACGGCCGCATCTCCGAC
CGCTCCCTTTCGCGCTACCTCCGTTTCTCATGGTTCTTCCGCCCCGTGCTGCAAAGGCTATCCGCCCTCTGCATGCAAAG
CGCCGAGGACGCAAGCCGCATCCAGGCCATAGGCGCGAGGCCTGAGACGGTCCACGTCGCGGGGAACCTGAAGTACGACA
TCCCGCTGCGGCCGAAGAACCCGGAGCAGGCGTCCGGGATCAAGGCGAAGTACGGCATCCCCGGAGGGGCTTTCGTCTTC
ACCGCCGCCAGCACCCACGAAGGTGAGGAGGTGTTCGTCCTGGAGGCGTACCGCATGCTCTTGTCGGCGCGGCCGGAGAG
TTTCCTCGTCCTCGCCCCGCGCCACCCGGAGCGCGCCGCGGGCGTCGCCGAACAGGTGAAAAAGAGCGGGTTCAGCTTCC
GGCGCCGCTCCCAGTTGGAGGCGGAACCCGCGCCGCAGCAGCCGGGAGAGGTGTTCATCCTGGATACGGTCGGCGAACTC
GCGGGCCTCTACGGCGCCTCGGACCTCGTTTTCGTCGGCGGTTCGCTGGTCCCGACCGGAGGACACAACCCGCTGGAGCC
CGCCGCCTGCGGCATCCCGGTCCTCTTCGGGCCGCACATGGAGAACTTCCGCGAGATCGCTGCCACCTTCCTCGCCAAGG
AAGCGGGCATTCAGCTCGTAGGAGCGGCTGAGCTGGGGGAGAAGCTGGTATCCCTTTCCGGGGACGCAGCCGGCCGGGAC
CGGATGGGGCGCAACGGAGCGGGGATCCTCGCCGAAAGCGCTGGCTCCACCACGCGTCACCTCGACGCGATGGCGGCATG
CCTGGGGGGAAGCCGTGGCTGA

Upstream 100 bases:

>100_bases
CTCTGGGAGAGGGGACACTGGATAGAACTGTAATTTGATCGCCTGCCAAGGCTTTGAGGCGCGGACAAAAGTCCGCATGC
CAGAGGGTGTTTTCGCTGCG

Downstream 100 bases:

>100_bases
TCTGGAATCCTATTTCAAGGAATTGATGGACGGCAAGCGCGCCGGGTGGAAGGACCGGCTCGCCCTCGCCGGACTGCGCC
TCGCTTCGCTCCCCTACGCC

Product: CMP-3-deoxy-D-manno-octulosonate--lipid A tetraacyldisaccharide 3-deoxy-D-manno-octulosonate transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIDFIYNLLLWLLLPLLVPYHAYRSLSRGRRTAFMERFGAIPEAELEPLKGKRTILVHAVSVGETLAAQPLLKGIRSRFP
EHRVVITNVTETGRGVALKSNSADLCIYFPFDYPFAVRAVLKKVSPDLVVIMETEIWPNFIKEAGRLGIPVLLANGRISD
RSLSRYLRFSWFFRPVLQRLSALCMQSAEDASRIQAIGARPETVHVAGNLKYDIPLRPKNPEQASGIKAKYGIPGGAFVF
TAASTHEGEEVFVLEAYRMLLSARPESFLVLAPRHPERAAGVAEQVKKSGFSFRRRSQLEAEPAPQQPGEVFILDTVGEL
AGLYGASDLVFVGGSLVPTGGHNPLEPAACGIPVLFGPHMENFREIAATFLAKEAGIQLVGAAELGEKLVSLSGDAAGRD
RMGRNGAGILAESAGSTTRHLDAMAACLGGSRG

Sequences:

>Translated_433_residues
MIDFIYNLLLWLLLPLLVPYHAYRSLSRGRRTAFMERFGAIPEAELEPLKGKRTILVHAVSVGETLAAQPLLKGIRSRFP
EHRVVITNVTETGRGVALKSNSADLCIYFPFDYPFAVRAVLKKVSPDLVVIMETEIWPNFIKEAGRLGIPVLLANGRISD
RSLSRYLRFSWFFRPVLQRLSALCMQSAEDASRIQAIGARPETVHVAGNLKYDIPLRPKNPEQASGIKAKYGIPGGAFVF
TAASTHEGEEVFVLEAYRMLLSARPESFLVLAPRHPERAAGVAEQVKKSGFSFRRRSQLEAEPAPQQPGEVFILDTVGEL
AGLYGASDLVFVGGSLVPTGGHNPLEPAACGIPVLFGPHMENFREIAATFLAKEAGIQLVGAAELGEKLVSLSGDAAGRD
RMGRNGAGILAESAGSTTRHLDAMAACLGGSRG
>Mature_433_residues
MIDFIYNLLLWLLLPLLVPYHAYRSLSRGRRTAFMERFGAIPEAELEPLKGKRTILVHAVSVGETLAAQPLLKGIRSRFP
EHRVVITNVTETGRGVALKSNSADLCIYFPFDYPFAVRAVLKKVSPDLVVIMETEIWPNFIKEAGRLGIPVLLANGRISD
RSLSRYLRFSWFFRPVLQRLSALCMQSAEDASRIQAIGARPETVHVAGNLKYDIPLRPKNPEQASGIKAKYGIPGGAFVF
TAASTHEGEEVFVLEAYRMLLSARPESFLVLAPRHPERAAGVAEQVKKSGFSFRRRSQLEAEPAPQQPGEVFILDTVGEL
AGLYGASDLVFVGGSLVPTGGHNPLEPAACGIPVLFGPHMENFREIAATFLAKEAGIQLVGAAELGEKLVSLSGDAAGRD
RMGRNGAGILAESAGSTTRHLDAMAACLGGSRG

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=430, Percent_Identity=39.5348837209302, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46970; Mature: 46970

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFIYNLLLWLLLPLLVPYHAYRSLSRGRRTAFMERFGAIPEAELEPLKGKRTILVHAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SVGETLAAQPLLKGIRSRFPEHRVVITNVTETGRGVALKSNSADLCIYFPFDYPFAVRAV
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEECCCCCHHHHHHH
LKKVSPDLVVIMETEIWPNFIKEAGRLGIPVLLANGRISDRSLSRYLRFSWFFRPVLQRL
HHHCCCCEEEEEECCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SALCMQSAEDASRIQAIGARPETVHVAGNLKYDIPLRPKNPEQASGIKAKYGIPGGAFVF
HHHHHHCCHHHHHHHHCCCCCCEEEEECCEEEECCCCCCCCCHHCCCEEECCCCCCEEEE
TAASTHEGEEVFVLEAYRMLLSARPESFLVLAPRHPERAAGVAEQVKKSGFSFRRRSQLE
EEECCCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCC
AEPAPQQPGEVFILDTVGELAGLYGASDLVFVGGSLVPTGGHNPLEPAACGIPVLFGPHM
CCCCCCCCCCEEEEECHHHHHHHCCCCCEEEECCEEECCCCCCCCCHHHCCCCEEECCCH
ENFREIAATFLAKEAGIQLVGAAELGEKLVSLSGDAAGRDRMGRNGAGILAESAGSTTRH
HHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHH
LDAMAACLGGSRG
HHHHHHHHCCCCC
>Mature Secondary Structure
MIDFIYNLLLWLLLPLLVPYHAYRSLSRGRRTAFMERFGAIPEAELEPLKGKRTILVHAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
SVGETLAAQPLLKGIRSRFPEHRVVITNVTETGRGVALKSNSADLCIYFPFDYPFAVRAV
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEECCCCCHHHHHHH
LKKVSPDLVVIMETEIWPNFIKEAGRLGIPVLLANGRISDRSLSRYLRFSWFFRPVLQRL
HHHCCCCEEEEEECCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SALCMQSAEDASRIQAIGARPETVHVAGNLKYDIPLRPKNPEQASGIKAKYGIPGGAFVF
HHHHHHCCHHHHHHHHCCCCCCEEEEECCEEEECCCCCCCCCHHCCCEEECCCCCCEEEE
TAASTHEGEEVFVLEAYRMLLSARPESFLVLAPRHPERAAGVAEQVKKSGFSFRRRSQLE
EEECCCCCCEEEHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCC
AEPAPQQPGEVFILDTVGELAGLYGASDLVFVGGSLVPTGGHNPLEPAACGIPVLFGPHM
CCCCCCCCCCEEEEECHHHHHHHCCCCCEEEECCEEECCCCCCCCCHHHCCCCEEECCCH
ENFREIAATFLAKEAGIQLVGAAELGEKLVSLSGDAAGRDRMGRNGAGILAESAGSTTRH
HHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHH
LDAMAACLGGSRG
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]