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The map label for this gene is htrB [H]

Identifier: 197117238

GI number: 197117238

Start: 1000843

End: 1001772

Strand: Direct

Name: htrB [H]

Synonym: Gbem_0846

Alternate gene names: 197117238

Gene position: 1000843-1001772 (Clockwise)

Preceding gene: 197117237

Following gene: 197117239

Centisome position: 21.69

GC content: 65.16

Gene sequence:

>930_bases
ATGTTGAAACAGATCCGATGGTACCTGGAGACCGCGCTGTTCGTTGCGGTCTCCTTTGCTGTGGCGCTCCTGCCGGACCG
GCTGGCGCTGGCCGCAGGACGGGGAATCGGCCGGCTCTTCTTTGCCCTGGTGCAGGGGCGGCGGCGGATAGCCATCGAAA
ACATCGCGGCGAGCCTCCCCTTTCTGGAAAGCCAGCCCGGCTGGCGCGGCGGACCCCCCGAACAGTTGGCGCGGGAAACC
TTCGAGAACCTGGGCTGCTGCGTGGTCGAGGTCTGCAAGCTCTACCGCGGCAAGGGACAGGGGCTCATCGACTCGGTCGA
ATTCAGGGGGCTGGAGCACTACGAGGCTGCGGCCGCCAAGGGGAAGGGTGTCGCCTTCATCACCGCCCACTGCGGCAACT
GGGAGCTTCTGGCCCTTTCCTTCGGCCAGCGCTACCACGACATCTCCGTAGTCGCCCGGCGCCAGGACAACCCGTACCTG
AACGCGATGATAGAAAAGATCCGCAAGGCGTACGGCAACGGCGTCATCTATAAGGACGGGGCGCTCCGCTCCATGTTCGC
AGCGCTCAAGAAGAAGGAGATCGTGGGGATTCTGATCGATCAGGCGGTGCACCCGGACGGCGGGATACTGGTCGACTTCC
TGGGGCGTCCGGCGTGGGCCATCCGGCTCCCGGCGCTCATAGGGCGCAAGAGCGGCGCACCGCTGGTGCCCGGTTTCATC
CATCGCGAGGGAACCAGGAACATCATCACCCTCTATCCGGAGTACCCCGTTTCCACCCTGGAGGACCCGGAGGAGGCTGC
GGCTGAGGACGCGCGGGGGCTGACGCGGTTCATCGAGGAATACGTGATACAGCACCCCACGCAGTGGTACTGGGTGCACA
AGCGCTGGAAGAATTCCCCAGCCGCGGCGGACCGGGCTGCCGGGAAATAG

Upstream 100 bases:

>100_bases
CTCTACAGCCGTCTGCACGGGATGCAGTTCAAGGCATAGGAAACGGAATAGAGGAACCCCCTTTCGGAAGGGGAGGGGGA
TTTGATTGAGAGGCTTTTCG

Downstream 100 bases:

>100_bases
CAAACTTCTCCCCTCGCCCTCTGGGAGAGGGGACACTGGATAGAACTGTAATTTGATCGCCTGCCAAGGCTTTGAGGCGC
GGACAAAAGTCCGCATGCCA

Product: lipid A biosynthesis lauroyl/myristoyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MLKQIRWYLETALFVAVSFAVALLPDRLALAAGRGIGRLFFALVQGRRRIAIENIAASLPFLESQPGWRGGPPEQLARET
FENLGCCVVEVCKLYRGKGQGLIDSVEFRGLEHYEAAAAKGKGVAFITAHCGNWELLALSFGQRYHDISVVARRQDNPYL
NAMIEKIRKAYGNGVIYKDGALRSMFAALKKKEIVGILIDQAVHPDGGILVDFLGRPAWAIRLPALIGRKSGAPLVPGFI
HREGTRNIITLYPEYPVSTLEDPEEAAAEDARGLTRFIEEYVIQHPTQWYWVHKRWKNSPAAADRAAGK

Sequences:

>Translated_309_residues
MLKQIRWYLETALFVAVSFAVALLPDRLALAAGRGIGRLFFALVQGRRRIAIENIAASLPFLESQPGWRGGPPEQLARET
FENLGCCVVEVCKLYRGKGQGLIDSVEFRGLEHYEAAAAKGKGVAFITAHCGNWELLALSFGQRYHDISVVARRQDNPYL
NAMIEKIRKAYGNGVIYKDGALRSMFAALKKKEIVGILIDQAVHPDGGILVDFLGRPAWAIRLPALIGRKSGAPLVPGFI
HREGTRNIITLYPEYPVSTLEDPEEAAAEDARGLTRFIEEYVIQHPTQWYWVHKRWKNSPAAADRAAGK
>Mature_309_residues
MLKQIRWYLETALFVAVSFAVALLPDRLALAAGRGIGRLFFALVQGRRRIAIENIAASLPFLESQPGWRGGPPEQLARET
FENLGCCVVEVCKLYRGKGQGLIDSVEFRGLEHYEAAAAKGKGVAFITAHCGNWELLALSFGQRYHDISVVARRQDNPYL
NAMIEKIRKAYGNGVIYKDGALRSMFAALKKKEIVGILIDQAVHPDGGILVDFLGRPAWAIRLPALIGRKSGAPLVPGFI
HREGTRNIITLYPEYPVSTLEDPEEAAAEDARGLTRFIEEYVIQHPTQWYWVHKRWKNSPAAADRAAGK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=302, Percent_Identity=25.4966887417219, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 34336; Mature: 34336

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQIRWYLETALFVAVSFAVALLPDRLALAAGRGIGRLFFALVQGRRRIAIENIAASLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCC
FLESQPGWRGGPPEQLARETFENLGCCVVEVCKLYRGKGQGLIDSVEFRGLEHYEAAAAK
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHCC
GKGVAFITAHCGNWELLALSFGQRYHDISVVARRQDNPYLNAMIEKIRKAYGNGVIYKDG
CCCEEEEEEECCCEEEEEEECCCHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCEEEECC
ALRSMFAALKKKEIVGILIDQAVHPDGGILVDFLGRPAWAIRLPALIGRKSGAPLVPGFI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECHHHHCCCCCCCCCCHHH
HREGTRNIITLYPEYPVSTLEDPEEAAAEDARGLTRFIEEYVIQHPTQWYWVHKRWKNSP
HCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCC
AAADRAAGK
CCCHHCCCC
>Mature Secondary Structure
MLKQIRWYLETALFVAVSFAVALLPDRLALAAGRGIGRLFFALVQGRRRIAIENIAASLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCC
FLESQPGWRGGPPEQLARETFENLGCCVVEVCKLYRGKGQGLIDSVEFRGLEHYEAAAAK
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHCC
GKGVAFITAHCGNWELLALSFGQRYHDISVVARRQDNPYLNAMIEKIRKAYGNGVIYKDG
CCCEEEEEEECCCEEEEEEECCCHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCEEEECC
ALRSMFAALKKKEIVGILIDQAVHPDGGILVDFLGRPAWAIRLPALIGRKSGAPLVPGFI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECHHHHCCCCCCCCCCHHH
HREGTRNIITLYPEYPVSTLEDPEEAAAEDARGLTRFIEEYVIQHPTQWYWVHKRWKNSP
HCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCC
AAADRAAGK
CCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]