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The map label for this gene is miaB

Identifier: 197117156

GI number: 197117156

Start: 896013

End: 897338

Strand: Direct

Name: miaB

Synonym: Gbem_0761

Alternate gene names: 197117156

Gene position: 896013-897338 (Clockwise)

Preceding gene: 197117152

Following gene: 308535187

Centisome position: 19.41

GC content: 61.46

Gene sequence:

>1326_bases
GTGAACCAAGCAAAAAAGCTGTACCTGGAAACCTTCGGTTGCCAGATGAATGTAAGTGATTCAGAGAAGATAGTTACCCT
GATGAAGGGGATGGGATACCAGCAGACGCAGGACCCGGTCGATGCCGACCTGGTTTTATTGAACACCTGCAGCATCAGGG
CTACCGCCGAACAGCGGGTCTACGGGCACTTGGGGAAGTTCAAGTCGATCAAGAAGACAAAGCCGGGGCTCATCATCGGC
GTTGGCGGCTGCGTGGCCCAGCAGGAGGGGGAAAAGCTCCTCAAGAAAGCGCCTTTCGTAAACCTTGTCTTCGGCACCCA
TAACCTGCACCTGCTGCAGGGAATGGTCGCGGCCGCCGAGGAAGGGAAGCGGAGCAGCCAGACCGACTTTCTCGACGACG
AAAAGCGGTTCGATCTCTTCCCGCACTCCGAGGCGGAAGGGGGAGTGACCCGGTTCGTCACCGTGATGCAGGGATGCGAT
AATTTCTGCGCCTACTGCATCGTCCCGCACGTGCGCGGCCGCGAGATCAGCAGGAGCGCCGCCAAGGTGGTCGAAGAGGT
ACGGGCCCTAGCCGATTCCGGCGTCACCGAGGTGACCCTTCTGGGACAGAACGTGAACTCTTATTGCTCCAAGCAGCCGG
GGGAGCCGGATTTCCCGGACCTTTTGCGCCTGGTGGCGCAGGTCGATGGCATCGAGCGTATCCGGTTCACCACCTCGCAT
CCCAAGGACATGTCGCCAAGGCTCATTGAGTGCTTCGCCGATCTTCCCAAGCTTGCCCCCCACATTCACCTTCCCGCCCA
GTCGGGAAGCGACCGGGTGCTTGAGCGGATGAACCGCGGCTACACGGCGCAGCAATACCTGGCCAAGGTGGCGGCGCTCA
AAGAGGCCTGCCCCGCGATCCAGTTCACCGGCGACATGATCGTCGGCTTTCCAGGCGAGGACGAGGCGGCCTTTCAGGAC
ACCATGGCATTGATGGAGCAGGTGCAGTACGCCGATCTCTTCTCCTTCATCTACTCGGCGCGCCCGGGAACCAAAGCCGC
CGAGTATGCGGACGATGCGACCCGTGCCGAGAAGCAGGGGCGCCTGGAAAGGTTGCAGGCGGCCCAGAAGAAGACCACTT
TGGCTAGGAACCGGAGCCTGGAGGGGACGGTGCAAAAGGTGCTGGTGGAGGGTTTGAGCAGCACAGGCGACTCCCTCTTC
GGCAGGACCGGCGGCAACCGCGGCACCGTCATGGCGGGGGACCCCTCCCTGGCCGGACGGGTGTTGGACGTGAAGATAGT
GGAAGGGTTGCAGACCCTGCTCAAGGGCGAGATCGTCCATGACTGA

Upstream 100 bases:

>100_bases
CATCCCCCCCGGCAATGGGCGTATCTGCCCAGCTAATAGGGGTTGACTGGGTGTGTCCAATTCCCGTACTATTCAACAAT
TTAATCATCAGGATAAACAC

Downstream 100 bases:

>100_bases
AACGAAAATGGGAGAAGCCAATATGTATCTGGAGATGAAGGTGTTCGGCTTCGCGCTAGATGCCATCGCGCAGATGCCGG
TGATCATATTGAAGGATACC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNQAKKLYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRVYGHLGKFKSIKKTKPGLIIG
VGGCVAQQEGEKLLKKAPFVNLVFGTHNLHLLQGMVAAAEEGKRSSQTDFLDDEKRFDLFPHSEAEGGVTRFVTVMQGCD
NFCAYCIVPHVRGREISRSAAKVVEEVRALADSGVTEVTLLGQNVNSYCSKQPGEPDFPDLLRLVAQVDGIERIRFTTSH
PKDMSPRLIECFADLPKLAPHIHLPAQSGSDRVLERMNRGYTAQQYLAKVAALKEACPAIQFTGDMIVGFPGEDEAAFQD
TMALMEQVQYADLFSFIYSARPGTKAAEYADDATRAEKQGRLERLQAAQKKTTLARNRSLEGTVQKVLVEGLSSTGDSLF
GRTGGNRGTVMAGDPSLAGRVLDVKIVEGLQTLLKGEIVHD

Sequences:

>Translated_441_residues
MNQAKKLYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRVYGHLGKFKSIKKTKPGLIIG
VGGCVAQQEGEKLLKKAPFVNLVFGTHNLHLLQGMVAAAEEGKRSSQTDFLDDEKRFDLFPHSEAEGGVTRFVTVMQGCD
NFCAYCIVPHVRGREISRSAAKVVEEVRALADSGVTEVTLLGQNVNSYCSKQPGEPDFPDLLRLVAQVDGIERIRFTTSH
PKDMSPRLIECFADLPKLAPHIHLPAQSGSDRVLERMNRGYTAQQYLAKVAALKEACPAIQFTGDMIVGFPGEDEAAFQD
TMALMEQVQYADLFSFIYSARPGTKAAEYADDATRAEKQGRLERLQAAQKKTTLARNRSLEGTVQKVLVEGLSSTGDSLF
GRTGGNRGTVMAGDPSLAGRVLDVKIVEGLQTLLKGEIVHD
>Mature_441_residues
MNQAKKLYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRVYGHLGKFKSIKKTKPGLIIG
VGGCVAQQEGEKLLKKAPFVNLVFGTHNLHLLQGMVAAAEEGKRSSQTDFLDDEKRFDLFPHSEAEGGVTRFVTVMQGCD
NFCAYCIVPHVRGREISRSAAKVVEEVRALADSGVTEVTLLGQNVNSYCSKQPGEPDFPDLLRLVAQVDGIERIRFTTSH
PKDMSPRLIECFADLPKLAPHIHLPAQSGSDRVLERMNRGYTAQQYLAKVAALKEACPAIQFTGDMIVGFPGEDEAAFQD
TMALMEQVQYADLFSFIYSARPGTKAAEYADDATRAEKQGRLERLQAAQKKTTLARNRSLEGTVQKVLVEGLSSTGDSLF
GRTGGNRGTVMAGDPSLAGRVLDVKIVEGLQTLLKGEIVHD

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=36.5904365904366, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=36.5904365904366, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI93277076, Length=454, Percent_Identity=27.3127753303965, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=45.124716553288, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787057, Length=428, Percent_Identity=25.9345794392523, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=423, Percent_Identity=39.9527186761229, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=261, Percent_Identity=29.5019157088123, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=37.3417721518987, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI19922432, Length=455, Percent_Identity=26.5934065934066, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_GEOBB (B5EE49)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137583.1
- ProteinModelPortal:   B5EE49
- SMR:   B5EE49
- GeneID:   6783594
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0761
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48222; Mature: 48222

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAKKLYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRV
CCCHHHHHHHHHCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
YGHLGKFKSIKKTKPGLIIGVGGCVAQQEGEKLLKKAPFVNLVFGTHNLHLLQGMVAAAE
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
EGKRSSQTDFLDDEKRFDLFPHSEAEGGVTRFVTVMQGCDNFCAYCIVPHVRGREISRSA
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AKVVEEVRALADSGVTEVTLLGQNVNSYCSKQPGEPDFPDLLRLVAQVDGIERIRFTTSH
HHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHEEEECCC
PKDMSPRLIECFADLPKLAPHIHLPAQSGSDRVLERMNRGYTAQQYLAKVAALKEACPAI
CCCCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCE
QFTGDMIVGFPGEDEAAFQDTMALMEQVQYADLFSFIYSARPGTKAAEYADDATRAEKQG
EECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RLERLQAAQKKTTLARNRSLEGTVQKVLVEGLSSTGDSLFGRTGGNRGTVMAGDPSLAGR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEECCCCCCCC
VLDVKIVEGLQTLLKGEIVHD
EEHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQAKKLYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRV
CCCHHHHHHHHHCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
YGHLGKFKSIKKTKPGLIIGVGGCVAQQEGEKLLKKAPFVNLVFGTHNLHLLQGMVAAAE
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
EGKRSSQTDFLDDEKRFDLFPHSEAEGGVTRFVTVMQGCDNFCAYCIVPHVRGREISRSA
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AKVVEEVRALADSGVTEVTLLGQNVNSYCSKQPGEPDFPDLLRLVAQVDGIERIRFTTSH
HHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHEEEECCC
PKDMSPRLIECFADLPKLAPHIHLPAQSGSDRVLERMNRGYTAQQYLAKVAALKEACPAI
CCCCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCE
QFTGDMIVGFPGEDEAAFQDTMALMEQVQYADLFSFIYSARPGTKAAEYADDATRAEKQG
EECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RLERLQAAQKKTTLARNRSLEGTVQKVLVEGLSSTGDSLFGRTGGNRGTVMAGDPSLAGR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEECCCCCCCC
VLDVKIVEGLQTLLKGEIVHD
EEHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA