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The map label for this gene is holA [H]

Identifier: 197117150

GI number: 197117150

Start: 888997

End: 889986

Strand: Direct

Name: holA [H]

Synonym: Gbem_0755

Alternate gene names: 197117150

Gene position: 888997-889986 (Clockwise)

Preceding gene: 308535186

Following gene: 197117152

Centisome position: 19.26

GC content: 61.72

Gene sequence:

>990_bases
ATGAAACCGGAAGAATTCAGCAAGGCGGTCGACAAGGGGGAACTCGCGTCCCTTTACCTTTTGTACGGGGACGAGCCGTA
CCTGGTGGAGCGCGCCGTGAAGAAACTCCTGGAGCGAGCCGTTGATCCCTCCTTCCGCGACTTCAACCTGAACGTCTTCT
ACGGCAACGAGTGCAAGGGGGACGAGGTCTTCAGCGCCGCGCAGACGCTGCCGATGTTCGCCGAGCGGCGGGTCGTGCTG
GTGAAGAAGGGGGGGGAGCTTTCCGCCGGCGCCATGGAGATACTCCTCTCCTACGTGCAGGATCCTTGTCCCGGGACCTG
CCTGATCTTGCAGGCGGAGAAGGTCGACGGGCGCAAGAAGTTCTACGCCGAGTTCAAGAAGCGCGGCGAGGCCGTCGAGT
TCAAGCGCCCCTATGAGAACCAGCTGGGACCCTACGTGCGCGACGAGGTGCGTGCCGCAGGGAAGAAGATAGATTCCGCA
GCCGCCGAGCTTCTGGCCTACCTGGTCGGCAACAACCTGCAGGAACTAGTCTCCCAGATCGAGAAACTCTGCATCTACTG
CGGCAAGAAGGAATCGATCGGCGTAGCCGACGTCAAGGCGATCGTCTCCGACACCAAGGTCGAGAGCGTCTTCGAGTTCA
CCGATGCCCTGGGGGCAAAGGAACTTCCCCGTGCGCTCAGGATGCTCACCTCCCTTTTGCAGGACGGCGAGGCGCCGCTG
CGCATCCTCGGCGCCGTTTCGCGCCATTACCGGCAGTTGTGGCAGATGCGGGAGCTTTTGGACCGCAGGCTTTCCCCCGC
CGAACTGGCGAAGGCGTCCGGGATCAACCCGTATTTCCTGAAGAAGGTGACCGATCAGGCGAGAAACTACACGGTGGGGG
AACTGAAGCAGGTTTTCGAGCGGATGCTGGAACTCGACCTGGCCTTCAAGTCGGGAGCCCTGGAAGGAGCGGCCTTCGAG
CGATTCGTCATGGACGCCTGCCGGAAGTAG

Upstream 100 bases:

>100_bases
GAAGCGGTCTCTGGCACCTTCCCCCCTACCCCGACCCTCCCCCGCAAGGGGGGAGGGAGCATGAGAGCGAATCAGGTAAC
GAAACGAAAGAGGGACAGGC

Downstream 100 bases:

>100_bases
CGAGTCGCTCACTCACCCAGACCCGAAAAATCCCCTCACCCTCTGGGAGAGGGTGGCCGCAAGGCCGGGTGAGGGAGGTG
CCATGAGCAAGAACAGGCGC

Product: DNA polymerase III subunit delta

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKPEEFSKAVDKGELASLYLLYGDEPYLVERAVKKLLERAVDPSFRDFNLNVFYGNECKGDEVFSAAQTLPMFAERRVVL
VKKGGELSAGAMEILLSYVQDPCPGTCLILQAEKVDGRKKFYAEFKKRGEAVEFKRPYENQLGPYVRDEVRAAGKKIDSA
AAELLAYLVGNNLQELVSQIEKLCIYCGKKESIGVADVKAIVSDTKVESVFEFTDALGAKELPRALRMLTSLLQDGEAPL
RILGAVSRHYRQLWQMRELLDRRLSPAELAKASGINPYFLKKVTDQARNYTVGELKQVFERMLELDLAFKSGALEGAAFE
RFVMDACRK

Sequences:

>Translated_329_residues
MKPEEFSKAVDKGELASLYLLYGDEPYLVERAVKKLLERAVDPSFRDFNLNVFYGNECKGDEVFSAAQTLPMFAERRVVL
VKKGGELSAGAMEILLSYVQDPCPGTCLILQAEKVDGRKKFYAEFKKRGEAVEFKRPYENQLGPYVRDEVRAAGKKIDSA
AAELLAYLVGNNLQELVSQIEKLCIYCGKKESIGVADVKAIVSDTKVESVFEFTDALGAKELPRALRMLTSLLQDGEAPL
RILGAVSRHYRQLWQMRELLDRRLSPAELAKASGINPYFLKKVTDQARNYTVGELKQVFERMLELDLAFKSGALEGAAFE
RFVMDACRK
>Mature_329_residues
MKPEEFSKAVDKGELASLYLLYGDEPYLVERAVKKLLERAVDPSFRDFNLNVFYGNECKGDEVFSAAQTLPMFAERRVVL
VKKGGELSAGAMEILLSYVQDPCPGTCLILQAEKVDGRKKFYAEFKKRGEAVEFKRPYENQLGPYVRDEVRAAGKKIDSA
AAELLAYLVGNNLQELVSQIEKLCIYCGKKESIGVADVKAIVSDTKVESVFEFTDALGAKELPRALRMLTSLLQDGEAPL
RILGAVSRHYRQLWQMRELLDRRLSPAELAKASGINPYFLKKVTDQARNYTVGELKQVFERMLELDLAFKSGALEGAAFE
RFVMDACRK

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Delta Subunit Seems To Interact With The Gamma Subunit To Transfer The B

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: 2.7.7.7

Molecular weight: Translated: 36964; Mature: 36964

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEEFSKAVDKGELASLYLLYGDEPYLVERAVKKLLERAVDPSFRDFNLNVFYGNECKG
CCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
DEVFSAAQTLPMFAERRVVLVKKGGELSAGAMEILLSYVQDPCPGTCLILQAEKVDGRKK
HHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHCCCHHH
FYAEFKKRGEAVEFKRPYENQLGPYVRDEVRAAGKKIDSAAAELLAYLVGNNLQELVSQI
HHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EKLCIYCGKKESIGVADVKAIVSDTKVESVFEFTDALGAKELPRALRMLTSLLQDGEAPL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHH
RILGAVSRHYRQLWQMRELLDRRLSPAELAKASGINPYFLKKVTDQARNYTVGELKQVFE
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
RMLELDLAFKSGALEGAAFERFVMDACRK
HHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPEEFSKAVDKGELASLYLLYGDEPYLVERAVKKLLERAVDPSFRDFNLNVFYGNECKG
CCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
DEVFSAAQTLPMFAERRVVLVKKGGELSAGAMEILLSYVQDPCPGTCLILQAEKVDGRKK
HHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHHCCCHHH
FYAEFKKRGEAVEFKRPYENQLGPYVRDEVRAAGKKIDSAAAELLAYLVGNNLQELVSQI
HHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EKLCIYCGKKESIGVADVKAIVSDTKVESVFEFTDALGAKELPRALRMLTSLLQDGEAPL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHH
RILGAVSRHYRQLWQMRELLDRRLSPAELAKASGINPYFLKKVTDQARNYTVGELKQVFE
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
RMLELDLAFKSGALEGAAFERFVMDACRK
HHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]