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The map label for this gene is pckA [H]

Identifier: 197117129

GI number: 197117129

Start: 865682

End: 867625

Strand: Reverse

Name: pckA [H]

Synonym: Gbem_0734

Alternate gene names: 197117129

Gene position: 867625-865682 (Counterclockwise)

Preceding gene: 197117131

Following gene: 197117128

Centisome position: 18.8

GC content: 63.43

Gene sequence:

>1944_bases
ATGGAAACGATGGAAATCGGCAGCACCATGCAGACCGCAGTCTCTTCCAAGCATGCGATGGAGTCATACCCAAAGGAGGG
AGTAAAAATGAGTTATGAGGTAAAGAACAAGCAGCTGCTCGCCTGGGTCAAGGAAGTAGCAGAGATGTGCCAGCCCGACA
GCATCCACTGGTGCGACGGGAGCGAGGAGGAGTACAACAGCCTGTGCGATCTGCTGGTCAAAGGCGGCACCTTCCGTAAG
CTGAACCCCGAGAAGCGCCCGAACAGCTACCTTGCCTGCTCCGATCCGGGCGACGTCGCCCGCGTCGAGGACCGCACCTT
CATCTGCAGCCTCGCCAAGCAGGATGCAGGTCCGACCAATAACTGGATGCCCCCCAAAGAGATGAAGAAGACCCTCACCG
GGCTCTTCACCGGCTGCATGAAGGGGCGCACCATGTACGTCATCCCCTTCAGCATGGGTCCCCTGGGCTCCAACATCGCC
AAGATCGGCGTCGAGATCTCCGACTCAGCCTACGTCGCCGTCAACATGAAGATCATGACCCGCATGGGTAAGAAGGTGGT
CGACATCCTGGGCGAGAACGGCGAGTTCGTCCCCTGCCTGCACTCGGTCGGCGCACCGCTCGCCGCGGGACAAAAGGACG
TCACCTGGCCCTGCAACAAGGAGAAGTACATCGTCCACTTCCCCGAAGAGCGCTCCATCTGGTCCTTCGGCTCCGGCTAC
GGCGGGAACGCCCTTCTGGGCAAGAAGTGCCTGGCCCTGAGGATCGCCTCCAAGATCGCCAAGGACGAAGGGTGGCTTGC
CGAGCACATGCTGATCCTGGGCCTCGAGAACCCGGAAGGGGAGAAGACCTACCTGGGCGCCGCCTTCCCGAGCGCCTGCG
GCAAGACCAACCTCTCCATGCTGGTCCCGCCCGACCACTTCAAGAACAGCGGCTGGAAGGTTTCCACCGTGGGCGACGAC
ATCGCCTGGATCAAGCCCGGCGCCGACGGCCAGCTCTACGCCATCAACCCGGAAGCCGGCTTCTTCGGCGTGGCCCCCGG
CACCTCGTTCAAGTCGAACCCGAACGCCATGCACTCCTGCGCCAAGAACACCATCTTCACCAACGTGGCGCTCACCCCGG
ACGGCGACGTCTGGTGGGAGGGGATGACCGACGAGCTCCCGGCGAAGCTGACCGACTGGCAGGGTAACGAGTGGACCGCA
GGATGCGGCAGGAACGCGGCGCACCCGAACTCCCGCTTCACCTCCCCGGCCAACCAGTGCCCCTCCATCGACCCGGCCTG
GGAAGATCCCAAAGGGGTGCCGATGAAGGCCTTCGTGTTCGGCGGCCGCCGCAACAACGTGATCCCGCTGGTGTACCAGT
CCTTCAACTGGAGCTACGGCGTCTACCTCGCGGCCACCATGGGTAGCGAGACCACCGCGGCTGCGGTCGGCGCCATCGGC
AACGTCCGCCGCGACCCGTTCGCTATGCTCCCCTTCACCGGGTACCACGTGGCCGACTACTTCAACCACTGGCTGCAGGT
CGGGCGCACCACCCCGAACCCGCCGCGCATCTTCTCGGTCAACTGGTTCAGGAAGGACGCCAACGGCAAGTTCCTCTGGC
CGGGCTTCGGCGACAACATGCGCATCCTCAAGTGGATCGCCGACCGCGCCAACGGCCGCGGAGCTTCCGTGGAAAGCCCG
CTGGGGTGGATGCCGCGCTACGAGGACATCGACTGGACCGGCCTCAACTCCGTCAGCCGCGAGCAGTACCACGAGCTCAT
GTCCATCGACCGCGACGTCTGGAAGGAGGAGATCCTCTCCCACGAGGAGCTGTTCGTGAAGATGTACGACCGCCTCCCCA
AAGAGTTCCTCCACATCCGTGAGATGATCCTCTCCGGCCTTTGGCGTTCCCCGGAGCATTGGGAGCAGTTCAACCCGCTC
TCCGCCGAAGGGTTCAACGACTAG

Upstream 100 bases:

>100_bases
AACACTAACCGCAGACCGGAATCAGCAGCGGACCCGGCGACAGGGGTTCCGTTCCGTCTCCTTTCGGTCTTCCTGCCAGT
AACGGCAGAGGAGGGCAGCC

Downstream 100 bases:

>100_bases
AGAAAGTTGAAAATCCAATGAAACAAGCAGGGGCCCCGGTCTTAACGACCGGGGCCCCTTTGCTTTGCCGCGCCAGTTGC
AAAGATACCCGCCCCTGCTA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
METMEIGSTMQTAVSSKHAMESYPKEGVKMSYEVKNKQLLAWVKEVAEMCQPDSIHWCDGSEEEYNSLCDLLVKGGTFRK
LNPEKRPNSYLACSDPGDVARVEDRTFICSLAKQDAGPTNNWMPPKEMKKTLTGLFTGCMKGRTMYVIPFSMGPLGSNIA
KIGVEISDSAYVAVNMKIMTRMGKKVVDILGENGEFVPCLHSVGAPLAAGQKDVTWPCNKEKYIVHFPEERSIWSFGSGY
GGNALLGKKCLALRIASKIAKDEGWLAEHMLILGLENPEGEKTYLGAAFPSACGKTNLSMLVPPDHFKNSGWKVSTVGDD
IAWIKPGADGQLYAINPEAGFFGVAPGTSFKSNPNAMHSCAKNTIFTNVALTPDGDVWWEGMTDELPAKLTDWQGNEWTA
GCGRNAAHPNSRFTSPANQCPSIDPAWEDPKGVPMKAFVFGGRRNNVIPLVYQSFNWSYGVYLAATMGSETTAAAVGAIG
NVRRDPFAMLPFTGYHVADYFNHWLQVGRTTPNPPRIFSVNWFRKDANGKFLWPGFGDNMRILKWIADRANGRGASVESP
LGWMPRYEDIDWTGLNSVSREQYHELMSIDRDVWKEEILSHEELFVKMYDRLPKEFLHIREMILSGLWRSPEHWEQFNPL
SAEGFND

Sequences:

>Translated_647_residues
METMEIGSTMQTAVSSKHAMESYPKEGVKMSYEVKNKQLLAWVKEVAEMCQPDSIHWCDGSEEEYNSLCDLLVKGGTFRK
LNPEKRPNSYLACSDPGDVARVEDRTFICSLAKQDAGPTNNWMPPKEMKKTLTGLFTGCMKGRTMYVIPFSMGPLGSNIA
KIGVEISDSAYVAVNMKIMTRMGKKVVDILGENGEFVPCLHSVGAPLAAGQKDVTWPCNKEKYIVHFPEERSIWSFGSGY
GGNALLGKKCLALRIASKIAKDEGWLAEHMLILGLENPEGEKTYLGAAFPSACGKTNLSMLVPPDHFKNSGWKVSTVGDD
IAWIKPGADGQLYAINPEAGFFGVAPGTSFKSNPNAMHSCAKNTIFTNVALTPDGDVWWEGMTDELPAKLTDWQGNEWTA
GCGRNAAHPNSRFTSPANQCPSIDPAWEDPKGVPMKAFVFGGRRNNVIPLVYQSFNWSYGVYLAATMGSETTAAAVGAIG
NVRRDPFAMLPFTGYHVADYFNHWLQVGRTTPNPPRIFSVNWFRKDANGKFLWPGFGDNMRILKWIADRANGRGASVESP
LGWMPRYEDIDWTGLNSVSREQYHELMSIDRDVWKEEILSHEELFVKMYDRLPKEFLHIREMILSGLWRSPEHWEQFNPL
SAEGFND
>Mature_647_residues
METMEIGSTMQTAVSSKHAMESYPKEGVKMSYEVKNKQLLAWVKEVAEMCQPDSIHWCDGSEEEYNSLCDLLVKGGTFRK
LNPEKRPNSYLACSDPGDVARVEDRTFICSLAKQDAGPTNNWMPPKEMKKTLTGLFTGCMKGRTMYVIPFSMGPLGSNIA
KIGVEISDSAYVAVNMKIMTRMGKKVVDILGENGEFVPCLHSVGAPLAAGQKDVTWPCNKEKYIVHFPEERSIWSFGSGY
GGNALLGKKCLALRIASKIAKDEGWLAEHMLILGLENPEGEKTYLGAAFPSACGKTNLSMLVPPDHFKNSGWKVSTVGDD
IAWIKPGADGQLYAINPEAGFFGVAPGTSFKSNPNAMHSCAKNTIFTNVALTPDGDVWWEGMTDELPAKLTDWQGNEWTA
GCGRNAAHPNSRFTSPANQCPSIDPAWEDPKGVPMKAFVFGGRRNNVIPLVYQSFNWSYGVYLAATMGSETTAAAVGAIG
NVRRDPFAMLPFTGYHVADYFNHWLQVGRTTPNPPRIFSVNWFRKDANGKFLWPGFGDNMRILKWIADRANGRGASVESP
LGWMPRYEDIDWTGLNSVSREQYHELMSIDRDVWKEEILSHEELFVKMYDRLPKEFLHIREMILSGLWRSPEHWEQFNPL
SAEGFND

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=614, Percent_Identity=50, Blast_Score=606, Evalue=1e-173,
Organism=Homo sapiens, GI66346721, Length=591, Percent_Identity=51.4382402707276, Blast_Score=605, Evalue=1e-173,
Organism=Homo sapiens, GI66346723, Length=376, Percent_Identity=54.5212765957447, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17555492, Length=586, Percent_Identity=51.0238907849829, Blast_Score=585, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71989645, Length=585, Percent_Identity=49.9145299145299, Blast_Score=574, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989658, Length=585, Percent_Identity=49.9145299145299, Blast_Score=573, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989653, Length=562, Percent_Identity=51.2455516014235, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI32564411, Length=538, Percent_Identity=52.9739776951673, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI32564407, Length=494, Percent_Identity=54.251012145749, Blast_Score=538, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71989649, Length=494, Percent_Identity=50.8097165991903, Blast_Score=495, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI32564409, Length=340, Percent_Identity=52.0588235294118, Blast_Score=357, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17540956, Length=635, Percent_Identity=23.1496062992126, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655085, Length=588, Percent_Identity=51.1904761904762, Blast_Score=593, Evalue=1e-169,
Organism=Drosophila melanogaster, GI24655082, Length=577, Percent_Identity=51.6464471403813, Blast_Score=582, Evalue=1e-166,
Organism=Drosophila melanogaster, GI161077226, Length=547, Percent_Identity=52.2851919561243, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24655088, Length=474, Percent_Identity=54.4303797468354, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 72088; Mature: 72088

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METMEIGSTMQTAVSSKHAMESYPKEGVKMSYEVKNKQLLAWVKEVAEMCQPDSIHWCDG
CCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEECCC
SEEEYNSLCDLLVKGGTFRKLNPEKRPNSYLACSDPGDVARVEDRTFICSLAKQDAGPTN
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCEEEHHHCCCCCCC
NWMPPKEMKKTLTGLFTGCMKGRTMYVIPFSMGPLGSNIAKIGVEISDSAYVAVNMKIMT
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCEEEEEEHHHHH
RMGKKVVDILGENGEFVPCLHSVGAPLAAGQKDVTWPCNKEKYIVHFPEERSIWSFGSGY
HHHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
GGNALLGKKCLALRIASKIAKDEGWLAEHMLILGLENPEGEKTYLGAAFPSACGKTNLSM
CCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCCEEEECCCCHHCCCCCCEE
LVPPDHFKNSGWKVSTVGDDIAWIKPGADGQLYAINPEAGFFGVAPGTSFKSNPNAMHSC
EECCHHHCCCCEEEEECCCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHH
AKNTIFTNVALTPDGDVWWEGMTDELPAKLTDWQGNEWTAGCGRNAAHPNSRFTSPANQC
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHC
PSIDPAWEDPKGVPMKAFVFGGRRNNVIPLVYQSFNWSYGVYLAATMGSETTAAAVGAIG
CCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHC
NVRRDPFAMLPFTGYHVADYFNHWLQVGRTTPNPPRIFSVNWFRKDANGKFLWPGFGDNM
CCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCH
RILKWIADRANGRGASVESPLGWMPRYEDIDWTGLNSVSREQYHELMSIDRDVWKEEILS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HEELFVKMYDRLPKEFLHIREMILSGLWRSPEHWEQFNPLSAEGFND
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
METMEIGSTMQTAVSSKHAMESYPKEGVKMSYEVKNKQLLAWVKEVAEMCQPDSIHWCDG
CCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEECCC
SEEEYNSLCDLLVKGGTFRKLNPEKRPNSYLACSDPGDVARVEDRTFICSLAKQDAGPTN
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCCCEEEHHHCCCCCCC
NWMPPKEMKKTLTGLFTGCMKGRTMYVIPFSMGPLGSNIAKIGVEISDSAYVAVNMKIMT
CCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCEEEEEEHHHHH
RMGKKVVDILGENGEFVPCLHSVGAPLAAGQKDVTWPCNKEKYIVHFPEERSIWSFGSGY
HHHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
GGNALLGKKCLALRIASKIAKDEGWLAEHMLILGLENPEGEKTYLGAAFPSACGKTNLSM
CCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCCEEEECCCCHHCCCCCCEE
LVPPDHFKNSGWKVSTVGDDIAWIKPGADGQLYAINPEAGFFGVAPGTSFKSNPNAMHSC
EECCHHHCCCCEEEEECCCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHH
AKNTIFTNVALTPDGDVWWEGMTDELPAKLTDWQGNEWTAGCGRNAAHPNSRFTSPANQC
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHC
PSIDPAWEDPKGVPMKAFVFGGRRNNVIPLVYQSFNWSYGVYLAATMGSETTAAAVGAIG
CCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHC
NVRRDPFAMLPFTGYHVADYFNHWLQVGRTTPNPPRIFSVNWFRKDANGKFLWPGFGDNM
CCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCH
RILKWIADRANGRGASVESPLGWMPRYEDIDWTGLNSVSREQYHELMSIDRDVWKEEILS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HEELFVKMYDRLPKEFLHIREMILSGLWRSPEHWEQFNPLSAEGFND
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA