Click here to switch to the map view.

The map label for this gene is pckA [H]

Identifier: 197117128

GI number: 197117128

Start: 863944

End: 865557

Strand: Reverse

Name: pckA [H]

Synonym: Gbem_0733

Alternate gene names: 197117128

Gene position: 865557-863944 (Counterclockwise)

Preceding gene: 197117129

Following gene: 197117127

Centisome position: 18.75

GC content: 63.14

Gene sequence:

>1614_bases
ATGAGAATCAAAAAGGGGGGCGCCGTGAGATTCAATGACATCACACGGGGAAGCGGTCTGGAGCAGCACGGTATCACTAA
CCCGAACCTCATCTACTGGACCTCCCCCACCTCGGTGCTTTACGAGCAGGTGGTAAGAAGAGGCGAGGGGCTCATCTCCC
ACCTGGGTGCTTTGACCGTAAAGACCGGCCACTATACCGGGCGCGCCGCCAACGACAAGTTCATCGTCGACGAACCGGAA
AGCAGCAGCCATATCAACTGGGGCAAGGTGAACCGCCCCTTCCCGCCGGAGAGTTTCGACCACCTCTACAAGAAGATGAC
GAACTACATGCAGGGGCGCGACCTCTTCGTGCAGGACTGCTTCGCCGGGGCGAGCCCGGAGCACCGGATCCCGGTGCGCA
TCATCTCCGAGCGCGCCTGGCACGCGCTATTCGCGCGCAACATGTTCCTGCGCGCGACCCCGGAGGAACTCCTCACCCAC
AACACCGAGTTCACCGTAATAGACCTCCCCGCCTTCCACGCGCAGCCCAAAGCCGACGGGACCAACTCCGAGGCCTTCAT
CATCATCAACTTCGCCCGGAAGCTCGTGATCATAGGCGGCACCAGCTACGCCGGCGAGATCAAGAAGTCAATATTCACCG
TGCTCAACTACCTCTTGCCGCAAAAAGGGGTGATGTCGATGCACTGCTCGGCCAACGCCGGGGAGAAAGGGGAGGTCGCC
ATCTTCTTCGGGCTCTCCGGCACCGGCAAGACCACCCTCTCCGCCGCCTCCAACCGGCTTCTCATCGGCGACGACGAGCA
CGGCTGGGACGACGCAGGCGTCTTCAACTTCGAGGGAGGGTGCTACGCCAAGGTGATCAACCTCTCCCCGCAGAGCGAAC
CGGAGATCTACCAGTGCACCAGGAAGTTCGGCACCATCCTGGAGAACGTCGCCATCGACACCATCTCGCGCCGCATCGAT
CTCGACGACGCCTCCTTCACCGAGAACACCCGGGCCTCCTACCCCATCACCCACATCCCCAACATCGTCGGCGCAGGTGC
CGGCGGGCACCCGAACCACGTCATCATGCTCACCTGCGACGCCTTCGGCGTGCTCCCCCCGATCGCCAGGCTCACCCCGG
CGCAGGCGATGTACCATTTCCTCTCCGGGTATACGGCGAAGGTGGCGGGGACCGAAGCCGGCGTTACCGAGCCGCAGGCA
ACCTTCTCCGCCTGCTTCGGAGCGCCGTTCATGGCGCTCAGGCCCTCTCTTTACGCCGACCTCCTGGGAAAGAAGATCGC
CTCTCACCAGGTTTCCTGCTGGCTTGTCAACACCGGCTGGAGCGGCGGCGGCCCCGGCGTCGGGGAAAGGATGAAGATCG
CCTACTCCCGAGCCCTAGTAAACGCCGCCCTGGACGGTACCCTCGGCGCCGGCAGCTTCGTCAAGGACCCGGTCTTCGGC
CTCGACATCCCCACCGCCTGTCCCGGCCTTCCCGCCGACATCCTCAATCCGAGGAACGTCTGGAGCGACAAGGGGGCCTA
CGACGCCACCGCCCAGAAACTGGTAGAGATGTTCCGTCACAACTTCGAGCAGTTCAAGAGCACCACGTCGCCGGAGATCG
CCTCCGTCCTTTAG

Upstream 100 bases:

>100_bases
AAGCAGGGGCCCCGGTCTTAACGACCGGGGCCCCTTTGCTTTGCCGCGCCAGTTGCAAAGATACCCGCCCCTGCTATCTT
ATTAGCTTGTTACAAATCCA

Downstream 100 bases:

>100_bases
CTGTAAAGCGGATGGCGCCGCTGTCACCAATTCGAAGCCGAGGAGGCTGCCATGAAAAAGGTACATTCAGAGATATGCGA
CACGACCGAGAGGAAAGAGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MRIKKGGAVRFNDITRGSGLEQHGITNPNLIYWTSPTSVLYEQVVRRGEGLISHLGALTVKTGHYTGRAANDKFIVDEPE
SSSHINWGKVNRPFPPESFDHLYKKMTNYMQGRDLFVQDCFAGASPEHRIPVRIISERAWHALFARNMFLRATPEELLTH
NTEFTVIDLPAFHAQPKADGTNSEAFIIINFARKLVIIGGTSYAGEIKKSIFTVLNYLLPQKGVMSMHCSANAGEKGEVA
IFFGLSGTGKTTLSAASNRLLIGDDEHGWDDAGVFNFEGGCYAKVINLSPQSEPEIYQCTRKFGTILENVAIDTISRRID
LDDASFTENTRASYPITHIPNIVGAGAGGHPNHVIMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEAGVTEPQA
TFSACFGAPFMALRPSLYADLLGKKIASHQVSCWLVNTGWSGGGPGVGERMKIAYSRALVNAALDGTLGAGSFVKDPVFG
LDIPTACPGLPADILNPRNVWSDKGAYDATAQKLVEMFRHNFEQFKSTTSPEIASVL

Sequences:

>Translated_537_residues
MRIKKGGAVRFNDITRGSGLEQHGITNPNLIYWTSPTSVLYEQVVRRGEGLISHLGALTVKTGHYTGRAANDKFIVDEPE
SSSHINWGKVNRPFPPESFDHLYKKMTNYMQGRDLFVQDCFAGASPEHRIPVRIISERAWHALFARNMFLRATPEELLTH
NTEFTVIDLPAFHAQPKADGTNSEAFIIINFARKLVIIGGTSYAGEIKKSIFTVLNYLLPQKGVMSMHCSANAGEKGEVA
IFFGLSGTGKTTLSAASNRLLIGDDEHGWDDAGVFNFEGGCYAKVINLSPQSEPEIYQCTRKFGTILENVAIDTISRRID
LDDASFTENTRASYPITHIPNIVGAGAGGHPNHVIMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEAGVTEPQA
TFSACFGAPFMALRPSLYADLLGKKIASHQVSCWLVNTGWSGGGPGVGERMKIAYSRALVNAALDGTLGAGSFVKDPVFG
LDIPTACPGLPADILNPRNVWSDKGAYDATAQKLVEMFRHNFEQFKSTTSPEIASVL
>Mature_537_residues
MRIKKGGAVRFNDITRGSGLEQHGITNPNLIYWTSPTSVLYEQVVRRGEGLISHLGALTVKTGHYTGRAANDKFIVDEPE
SSSHINWGKVNRPFPPESFDHLYKKMTNYMQGRDLFVQDCFAGASPEHRIPVRIISERAWHALFARNMFLRATPEELLTH
NTEFTVIDLPAFHAQPKADGTNSEAFIIINFARKLVIIGGTSYAGEIKKSIFTVLNYLLPQKGVMSMHCSANAGEKGEVA
IFFGLSGTGKTTLSAASNRLLIGDDEHGWDDAGVFNFEGGCYAKVINLSPQSEPEIYQCTRKFGTILENVAIDTISRRID
LDDASFTENTRASYPITHIPNIVGAGAGGHPNHVIMLTCDAFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEAGVTEPQA
TFSACFGAPFMALRPSLYADLLGKKIASHQVSCWLVNTGWSGGGPGVGERMKIAYSRALVNAALDGTLGAGSFVKDPVFG
LDIPTACPGLPADILNPRNVWSDKGAYDATAQKLVEMFRHNFEQFKSTTSPEIASVL

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=533, Percent_Identity=46.9043151969981, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322950, Length=506, Percent_Identity=47.2332015810277, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58378; Mature: 58378

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKKGGAVRFNDITRGSGLEQHGITNPNLIYWTSPTSVLYEQVVRRGEGLISHLGALTV
CCCCCCCCEEECCCCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHCEEEE
KTGHYTGRAANDKFIVDEPESSSHINWGKVNRPFPPESFDHLYKKMTNYMQGRDLFVQDC
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
FAGASPEHRIPVRIISERAWHALFARNMFLRATPEELLTHNTEFTVIDLPAFHAQPKADG
HCCCCCCCCCCCEEHHHHHHHHHHHHCHHEECCHHHHHHCCCCEEEEECCCCCCCCCCCC
TNSEAFIIINFARKLVIIGGTSYAGEIKKSIFTVLNYLLPQKGVMSMHCSANAGEKGEVA
CCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEE
IFFGLSGTGKTTLSAASNRLLIGDDEHGWDDAGVFNFEGGCYAKVINLSPQSEPEIYQCT
EEEECCCCCCEEHHHCCCEEEECCCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHH
RKFGTILENVAIDTISRRIDLDDASFTENTRASYPITHIPNIVGAGAGGHPNHVIMLTCD
HHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEEEEC
AFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEAGVTEPQATFSACFGAPFMALRPSLYAD
CCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LLGKKIASHQVSCWLVNTGWSGGGPGVGERMKIAYSRALVNAALDGTLGAGSFVKDPVFG
HHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCC
LDIPTACPGLPADILNPRNVWSDKGAYDATAQKLVEMFRHNFEQFKSTTSPEIASVL
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MRIKKGGAVRFNDITRGSGLEQHGITNPNLIYWTSPTSVLYEQVVRRGEGLISHLGALTV
CCCCCCCCEEECCCCCCCCCHHCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHCEEEE
KTGHYTGRAANDKFIVDEPESSSHINWGKVNRPFPPESFDHLYKKMTNYMQGRDLFVQDC
ECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
FAGASPEHRIPVRIISERAWHALFARNMFLRATPEELLTHNTEFTVIDLPAFHAQPKADG
HCCCCCCCCCCCEEHHHHHHHHHHHHCHHEECCHHHHHHCCCCEEEEECCCCCCCCCCCC
TNSEAFIIINFARKLVIIGGTSYAGEIKKSIFTVLNYLLPQKGVMSMHCSANAGEKGEVA
CCCCEEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEE
IFFGLSGTGKTTLSAASNRLLIGDDEHGWDDAGVFNFEGGCYAKVINLSPQSEPEIYQCT
EEEECCCCCCEEHHHCCCEEEECCCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHH
RKFGTILENVAIDTISRRIDLDDASFTENTRASYPITHIPNIVGAGAGGHPNHVIMLTCD
HHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEEEEC
AFGVLPPIARLTPAQAMYHFLSGYTAKVAGTEAGVTEPQATFSACFGAPFMALRPSLYAD
CCCCCCCHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
LLGKKIASHQVSCWLVNTGWSGGGPGVGERMKIAYSRALVNAALDGTLGAGSFVKDPVFG
HHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCC
LDIPTACPGLPADILNPRNVWSDKGAYDATAQKLVEMFRHNFEQFKSTTSPEIASVL
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA