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The map label for this gene is rsbU [H]

Identifier: 197117126

GI number: 197117126

Start: 862461

End: 863651

Strand: Direct

Name: rsbU [H]

Synonym: Gbem_0731

Alternate gene names: 197117126

Gene position: 862461-863651 (Clockwise)

Preceding gene: 197117123

Following gene: 197117130

Centisome position: 18.69

GC content: 60.37

Gene sequence:

>1191_bases
ATGCGTACGACAATCCTCGTAGTAGATGACAGCAAGTCTATGCGCAGGATTATTGTGGACTCCCTTAAGGACGAGGGCTA
TGAATGCCGGTCCGCGGAGGATGTGGACACCGCCCTGATCGCTTTGGACGGGTGCGAGTTCAGTCTTGTGCTCTGCGACA
TCGAGATGCCTGGGCGCAACGGCATCTTCCTGCTCAACGAGATCCGCACCACACGCCCAGACACCTACGTCGTCATGCTG
ACCGGGGTCACCGACCCGGAGACGGTGATGCATTGCATCCACTTAGGCGCAAACGATTACCTGATGAAGCCGTTTACCTT
GGAGCGCCTGCTGCTCACGGTGAGGAATGCTCTCCAACAGCGAAAGCTCTACCTTGAGCACAGGTCATATCAGGAAACCC
TGGAGCAGAAGGTTCTGGAGCAAACCGAGCAGATACGCGCAAGCCTGTTGGAAAAAGCGCTCATCACCAAGGAGATGGAG
ATCGCCGGGGCCATACACGCGGCACTGGTCCCGCGGTTCATACCTTCTACCCCGCGCCTCTCCTTTGCCGCCCACTATCT
CTCAGCCGGCCCTCTGGGAGGAGACTATTTCGACATATTCCACCGTGGCGACGGCTTGGTGGACGTTGTCATCGCCGATG
TCGCAGGCCATAACATAGGGTCGGCGCTTATAGTGGCGCAGTTGCGCGGCGCCTTGCGGGTGCAGGCGTCGGCAGCGCGC
CTTGGCTGCGCGGAGATGCTGAAGCTGATCAACGAATCCTTTTACGAAGATCTGACCGGCGCCGAAATCTTCGTGAGCAT
GCTGTACCTCCGCCTGGACGAGGTGAGCATGCAGCTGCAATACGCCTGCGCCGGCCACAACCCCGGCATCATGCTCCGTG
CCGACGGCTCCGTGCTGGAACTCGACGCCGAGGGGATGATCATCGGCGTGGCGCGCGAGGTTGATTTCGAGGAGAAAGCG
CTTCCCCTGGAGGCGGGTAGCCGCCTGATGCTTTATACCGACGGGATAGTGGAGGCGGAAAACGGCGCAGGTGAGCTGTT
CGGGCTTGCCAAGTTTGTCGCATCGCTTAGCGAGGCGCGGGAGGGGGAGCCGGGCGTGCTGGTCGAGGCTGCGCTCTCTT
CGATGAAGCGCTTCACCTCGGCCGCGCCGCTAAAGGACGACCTGACGGTCGTGGTGGTGGGAGTCTCATAA

Upstream 100 bases:

>100_bases
ATCTATCTGGTAACCACGCTAATGTCCGGGCCGTTGCCTGCCGATAAGTCCAAGTAACCTCCTTTCACTCATTCCGCATC
GTCATTTCCAGGTGGTTTCT

Downstream 100 bases:

>100_bases
AGGAAAGGCCGCCCTGAATGCAGGACGGCCTTTCTTCTTTGTCTTGCTCTCAGGAAATCATCTCGCAATCATCCTCGGGC
CAGCTGTCGAGGCTGCCATC

Product: response receiver phosphatase, PP2C_SIG domain-containing protein

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRTTILVVDDSKSMRRIIVDSLKDEGYECRSAEDVDTALIALDGCEFSLVLCDIEMPGRNGIFLLNEIRTTRPDTYVVML
TGVTDPETVMHCIHLGANDYLMKPFTLERLLLTVRNALQQRKLYLEHRSYQETLEQKVLEQTEQIRASLLEKALITKEME
IAGAIHAALVPRFIPSTPRLSFAAHYLSAGPLGGDYFDIFHRGDGLVDVVIADVAGHNIGSALIVAQLRGALRVQASAAR
LGCAEMLKLINESFYEDLTGAEIFVSMLYLRLDEVSMQLQYACAGHNPGIMLRADGSVLELDAEGMIIGVAREVDFEEKA
LPLEAGSRLMLYTDGIVEAENGAGELFGLAKFVASLSEAREGEPGVLVEAALSSMKRFTSAAPLKDDLTVVVVGVS

Sequences:

>Translated_396_residues
MRTTILVVDDSKSMRRIIVDSLKDEGYECRSAEDVDTALIALDGCEFSLVLCDIEMPGRNGIFLLNEIRTTRPDTYVVML
TGVTDPETVMHCIHLGANDYLMKPFTLERLLLTVRNALQQRKLYLEHRSYQETLEQKVLEQTEQIRASLLEKALITKEME
IAGAIHAALVPRFIPSTPRLSFAAHYLSAGPLGGDYFDIFHRGDGLVDVVIADVAGHNIGSALIVAQLRGALRVQASAAR
LGCAEMLKLINESFYEDLTGAEIFVSMLYLRLDEVSMQLQYACAGHNPGIMLRADGSVLELDAEGMIIGVAREVDFEEKA
LPLEAGSRLMLYTDGIVEAENGAGELFGLAKFVASLSEAREGEPGVLVEAALSSMKRFTSAAPLKDDLTVVVVGVS
>Mature_396_residues
MRTTILVVDDSKSMRRIIVDSLKDEGYECRSAEDVDTALIALDGCEFSLVLCDIEMPGRNGIFLLNEIRTTRPDTYVVML
TGVTDPETVMHCIHLGANDYLMKPFTLERLLLTVRNALQQRKLYLEHRSYQETLEQKVLEQTEQIRASLLEKALITKEME
IAGAIHAALVPRFIPSTPRLSFAAHYLSAGPLGGDYFDIFHRGDGLVDVVIADVAGHNIGSALIVAQLRGALRVQASAAR
LGCAEMLKLINESFYEDLTGAEIFVSMLYLRLDEVSMQLQYACAGHNPGIMLRADGSVLELDAEGMIIGVAREVDFEEKA
LPLEAGSRLMLYTDGIVEAENGAGELFGLAKFVASLSEAREGEPGVLVEAALSSMKRFTSAAPLKDDLTVVVVGVS

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=35.3846153846154, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790299, Length=130, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=36.8421052631579, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082012, Length=131, Percent_Identity=30.5343511450382, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 43329; Mature: 43329

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTILVVDDSKSMRRIIVDSLKDEGYECRSAEDVDTALIALDGCEFSLVLCDIEMPGRN
CCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCC
GIFLLNEIRTTRPDTYVVMLTGVTDPETVMHCIHLGANDYLMKPFTLERLLLTVRNALQQ
CEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHH
RKLYLEHRSYQETLEQKVLEQTEQIRASLLEKALITKEMEIAGAIHAALVPRFIPSTPRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SFAAHYLSAGPLGGDYFDIFHRGDGLVDVVIADVAGHNIGSALIVAQLRGALRVQASAAR
HHHHHHHHCCCCCCCHHEEEECCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEHHHHH
LGCAEMLKLINESFYEDLTGAEIFVSMLYLRLDEVSMQLQYACAGHNPGIMLRADGSVLE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCEEE
LDAEGMIIGVAREVDFEEKALPLEAGSRLMLYTDGIVEAENGAGELFGLAKFVASLSEAR
ECCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHC
EGEPGVLVEAALSSMKRFTSAAPLKDDLTVVVVGVS
CCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRTTILVVDDSKSMRRIIVDSLKDEGYECRSAEDVDTALIALDGCEFSLVLCDIEMPGRN
CCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCC
GIFLLNEIRTTRPDTYVVMLTGVTDPETVMHCIHLGANDYLMKPFTLERLLLTVRNALQQ
CEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHH
RKLYLEHRSYQETLEQKVLEQTEQIRASLLEKALITKEMEIAGAIHAALVPRFIPSTPRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SFAAHYLSAGPLGGDYFDIFHRGDGLVDVVIADVAGHNIGSALIVAQLRGALRVQASAAR
HHHHHHHHCCCCCCCHHEEEECCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEHHHHH
LGCAEMLKLINESFYEDLTGAEIFVSMLYLRLDEVSMQLQYACAGHNPGIMLRADGSVLE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCEEE
LDAEGMIIGVAREVDFEEKALPLEAGSRLMLYTDGIVEAENGAGELFGLAKFVASLSEAR
ECCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHC
EGEPGVLVEAALSSMKRFTSAAPLKDDLTVVVVGVS
CCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]