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The map label for this gene is hybA [C]

Identifier: 197117061

GI number: 197117061

Start: 789931

End: 790839

Strand: Reverse

Name: hybA [C]

Synonym: Gbem_0665

Alternate gene names: 197117061

Gene position: 790839-789931 (Counterclockwise)

Preceding gene: 197117062

Following gene: 197117060

Centisome position: 17.14

GC content: 60.51

Gene sequence:

>909_bases
ATGAAACGAAGAAACTTCCTGAAGGCGTGCCTGATTGGCGGGACCACCATAGCGATGGGCAAGACCAACAAGGGGTGGGC
AAGTGGTTCCTTCGAAGGATACCCGGAATCGATGGGGGTCCTGGTGGATACCACCCGCTGCATCGGCTGCCGCAGCTGCG
AGGCTGCCTGCAACAAGGAACAGAAACTGCCGGAGCCGGACACCTCCTTCGACGACAAGTCGGTATTCGAGGAGCCCCGC
CGCCCGACGGCCAAGGCGTACACGGTGGTGAACCAGTACGAGAAGGAAAAGCCCGGAGGCCCCGTCTACCGCAAGGTGCA
GTGCAACCACTGCAACGAACCGGCCTGCCTCACCTCGTGCTTCGTGAACGCCTATACGAAGACCAAGGAGGGGGCGGTCA
TCTACAACTCCAAGGTCTGCGTCGGCTGCCGCAACTGCATGATCGCCTGCCCCTTCAACGTCCCGGGGTACGACTACGAA
AGCGTCACCAACCCGGTGATCAAGAAATGCATCCTTTGCTACGACACCCGCCTCAAGGTGGGCAAACCCCCGGCATGCGT
CGACATCTGCCCTCAAGAGGCGCTCACCTTCGGGCACCGGGCCGACCTGATCAAGATCGCGCACGAGCGTATCCGCTCGA
ACCCCGACCGCTACCTAGACCATGTCTACGGTGAAAAGGAGGTCGGCGGCACCAGCTGGCTCTACCTCTCCGGCACCGAG
TTCGACAAGATCGGTTTCGACCCGTCGCTCAGCAACGACCCGATCATTTCCAACGTGAAGGACTTCCTCGGCATGGTGCC
CATGGTGCTTTCCATCTGGCCGGCACTCTTTTCCGGGATCCACCTCCTCTCCAAACACGAATCGGGGGGAGAGGGACATG
ACACCGAATCCAAAGAGGAGGAGCCCTAA

Upstream 100 bases:

>100_bases
GTTCAACAGCTCTTCGCGACTGGGGGCCAAACCCGCCCATGCCAAGAAGCACTAGAATCGACATCGATAGCAGCTCATTC
ATGATTTGAGGAGGACGCTG

Downstream 100 bases:

>100_bases
CCATGAAAAACATGATTAAACACGGCTTGGCCGTCGTCGATTCGCCCTATGACGAGTCCGGGAAGAAGCGGACCGTGGTC
CAGAAGCTCCTTCTGGGGCT

Product: hydrogenase 2, 4Fe-4S ferredoxin-type protein

Products: NA

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKRRNFLKACLIGGTTIAMGKTNKGWASGSFEGYPESMGVLVDTTRCIGCRSCEAACNKEQKLPEPDTSFDDKSVFEEPR
RPTAKAYTVVNQYEKEKPGGPVYRKVQCNHCNEPACLTSCFVNAYTKTKEGAVIYNSKVCVGCRNCMIACPFNVPGYDYE
SVTNPVIKKCILCYDTRLKVGKPPACVDICPQEALTFGHRADLIKIAHERIRSNPDRYLDHVYGEKEVGGTSWLYLSGTE
FDKIGFDPSLSNDPIISNVKDFLGMVPMVLSIWPALFSGIHLLSKHESGGEGHDTESKEEEP

Sequences:

>Translated_302_residues
MKRRNFLKACLIGGTTIAMGKTNKGWASGSFEGYPESMGVLVDTTRCIGCRSCEAACNKEQKLPEPDTSFDDKSVFEEPR
RPTAKAYTVVNQYEKEKPGGPVYRKVQCNHCNEPACLTSCFVNAYTKTKEGAVIYNSKVCVGCRNCMIACPFNVPGYDYE
SVTNPVIKKCILCYDTRLKVGKPPACVDICPQEALTFGHRADLIKIAHERIRSNPDRYLDHVYGEKEVGGTSWLYLSGTE
FDKIGFDPSLSNDPIISNVKDFLGMVPMVLSIWPALFSGIHLLSKHESGGEGHDTESKEEEP
>Mature_302_residues
MKRRNFLKACLIGGTTIAMGKTNKGWASGSFEGYPESMGVLVDTTRCIGCRSCEAACNKEQKLPEPDTSFDDKSVFEEPR
RPTAKAYTVVNQYEKEKPGGPVYRKVQCNHCNEPACLTSCFVNAYTKTKEGAVIYNSKVCVGCRNCMIACPFNVPGYDYE
SVTNPVIKKCILCYDTRLKVGKPPACVDICPQEALTFGHRADLIKIAHERIRSNPDRYLDHVYGEKEVGGTSWLYLSGTE
FDKIGFDPSLSNDPIISNVKDFLGMVPMVLSIWPALFSGIHLLSKHESGGEGHDTESKEEEP

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=333, Percent_Identity=30.6306306306306, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787749, Length=271, Percent_Identity=31.3653136531365, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790326, Length=280, Percent_Identity=30.3571428571429, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787122, Length=167, Percent_Identity=37.125748502994, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787872, Length=160, Percent_Identity=36.875, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI2367345, Length=228, Percent_Identity=30.2631578947368, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI226510944, Length=196, Percent_Identity=28.0612244897959, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082180, Length=176, Percent_Identity=30.1136363636364, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367245, Length=182, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789079, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=175, Percent_Identity=30.8571428571429, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788811, Length=179, Percent_Identity=26.2569832402235, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 33462; Mature: 33462

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRNFLKACLIGGTTIAMGKTNKGWASGSFEGYPESMGVLVDTTRCIGCRSCEAACNKE
CCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCHHHHHCCH
QKLPEPDTSFDDKSVFEEPRRPTAKAYTVVNQYEKEKPGGPVYRKVQCNHCNEPACLTSC
HCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
FVNAYTKTKEGAVIYNSKVCVGCRNCMIACPFNVPGYDYESVTNPVIKKCILCYDTRLKV
HHHHHHCCCCCEEEECCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GKPPACVDICPQEALTFGHRADLIKIAHERIRSNPDRYLDHVYGEKEVGGTSWLYLSGTE
CCCCCHHCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEEECCC
FDKIGFDPSLSNDPIISNVKDFLGMVPMVLSIWPALFSGIHLLSKHESGGEGHDTESKEE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EP
CC
>Mature Secondary Structure
MKRRNFLKACLIGGTTIAMGKTNKGWASGSFEGYPESMGVLVDTTRCIGCRSCEAACNKE
CCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCHHHHHCCH
QKLPEPDTSFDDKSVFEEPRRPTAKAYTVVNQYEKEKPGGPVYRKVQCNHCNEPACLTSC
HCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
FVNAYTKTKEGAVIYNSKVCVGCRNCMIACPFNVPGYDYESVTNPVIKKCILCYDTRLKV
HHHHHHCCCCCEEEECCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GKPPACVDICPQEALTFGHRADLIKIAHERIRSNPDRYLDHVYGEKEVGGTSWLYLSGTE
CCCCCHHCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEEEEECCC
FDKIGFDPSLSNDPIISNVKDFLGMVPMVLSIWPALFSGIHLLSKHESGGEGHDTESKEE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]