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The map label for this gene is hybB [C]

Identifier: 197117060

GI number: 197117060

Start: 788573

End: 789928

Strand: Reverse

Name: hybB [C]

Synonym: Gbem_0664

Alternate gene names: 197117060

Gene position: 789928-788573 (Counterclockwise)

Preceding gene: 197117061

Following gene: 197117059

Centisome position: 17.12

GC content: 62.91

Gene sequence:

>1356_bases
ATGAAAAACATGATTAAACACGGCTTGGCCGTCGTCGATTCGCCCTATGACGAGTCCGGGAAGAAGCGGACCGTGGTCCA
GAAGCTCCTTCTGGGGCTCTCCCCGAGCCAGTACCTGTTGCAGGCGTTCAGAAACCCCTTCAACTGGATACTCGCCGCCA
TCTTCGCCATCGGCATCCCGATCCTTTTGGGGCGCTTCGTCTTCGGGCTCGCCTGGGCCACCAGCGGCTCCAACGACTAC
CCCTGGGGCCTTTTCCTGGGCTTCGGCCTCTTCGCCATGGTGCCGCTATCCTCCTCGGGGTTCCAGTTGGGAACCGCCTG
CGAGATCTTCGGCCGCCACGACCTGGAGCCGATCGAGCGGCTCGCCCTTTTGAACGGGCTTTTGGGTTACTTCTTCGCGG
TAGTCTACCTGCTGGCGGACCTGGGGCAGCCCTGGCGGCTCCCCTACCCGATGGTGGTCTCCTTCGGCCCGGCGGCGGTC
CTCTTCCTGGTCGCCTGGCACGTCGCCACCTACCTCTCGGTGCAGGTGGCCGAGGTCTCCACCGCCTTCTTCGAGTGGAT
CGGGTTCCCGGCGGGCAAGCGCGGGGTGCGCAGGATCACCTTGGGGCTCACCGTTTCCGGGATCATCCTCTCCACGCTGC
ACCAGGGGGCGTTAGGGGCGCTCTTCACCTACGCGCCCGGCAAGGTGCACCCGCTTTGGTATTCCTCATCCTTCCAGTGG
CTGCACTTCTTCGTGTCGTCCCTTCCCGGGGGCCTTTGCATGGTGATCACGGTCAGCACCATCGCCTACAAGTTCATGGG
ATGGCGCACCGACAGAAGGTTCAAGGAAGCCCTCGACCGCTGCACCGTGACCCTCGCCAAAGGGGCCTGCATGGGGATCG
CGACCTACATCACCATCAAGCTCATCGGCATCGCCCATGACAACAAGTGGAGCTACCTGGACAGCGGTTGGGGGCAGTGG
TTCATGCTCGAGATCATCGTCGGCTTCATGCTGCCGCTCGTGCTGATGACCTACGGCGTGCACACCCGCAAGATGGGCGT
CATCCGCCTGGGCGCCTTCGCCACCGTCTTCGGCGTGGTGCTGAATCGGGTCAACACCGCCATCATCACCTTCAACTGGA
AACTGCCGGAAAGGGAGATCCCGCCCTGGCGCGAGGTCGTCATCAGCCTGACCATCTTCGCCATCTACATCGTGGTGTAC
CGCTTCATCCTGTACCGCCTACCCATCCTCTACAAGTGGAGGCAGCAGGAGGAAGAGGTCACGGTAACCGAGGCGGTGAC
CGCACCGGCGGCTGGCCACCGCGAGGCGAGCCCCGTGGCCGTCGCCTCCTACAGCAAAATGACCAAATTGGATTGA

Upstream 100 bases:

>100_bases
CCATCTGGCCGGCACTCTTTTCCGGGATCCACCTCCTCTCCAAACACGAATCGGGGGGAGAGGGACATGACACCGAATCC
AAAGAGGAGGAGCCCTAACC

Downstream 100 bases:

>100_bases
CCCTCGCGAAACCGGCGGCGGCTCAAAGGACGAAACGAGGTCCGGCGAGCCGCCAGGGTAAGGCGCGCGAAGAGCGCAAG
GGAGATGGCGCCATGTTCGA

Product: hydrogenase 2, cytochrome b-type protein

Products: NA

Alternate protein names: HMC operon ORF 3 [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKNMIKHGLAVVDSPYDESGKKRTVVQKLLLGLSPSQYLLQAFRNPFNWILAAIFAIGIPILLGRFVFGLAWATSGSNDY
PWGLFLGFGLFAMVPLSSSGFQLGTACEIFGRHDLEPIERLALLNGLLGYFFAVVYLLADLGQPWRLPYPMVVSFGPAAV
LFLVAWHVATYLSVQVAEVSTAFFEWIGFPAGKRGVRRITLGLTVSGIILSTLHQGALGALFTYAPGKVHPLWYSSSFQW
LHFFVSSLPGGLCMVITVSTIAYKFMGWRTDRRFKEALDRCTVTLAKGACMGIATYITIKLIGIAHDNKWSYLDSGWGQW
FMLEIIVGFMLPLVLMTYGVHTRKMGVIRLGAFATVFGVVLNRVNTAIITFNWKLPEREIPPWREVVISLTIFAIYIVVY
RFILYRLPILYKWRQQEEEVTVTEAVTAPAAGHREASPVAVASYSKMTKLD

Sequences:

>Translated_451_residues
MKNMIKHGLAVVDSPYDESGKKRTVVQKLLLGLSPSQYLLQAFRNPFNWILAAIFAIGIPILLGRFVFGLAWATSGSNDY
PWGLFLGFGLFAMVPLSSSGFQLGTACEIFGRHDLEPIERLALLNGLLGYFFAVVYLLADLGQPWRLPYPMVVSFGPAAV
LFLVAWHVATYLSVQVAEVSTAFFEWIGFPAGKRGVRRITLGLTVSGIILSTLHQGALGALFTYAPGKVHPLWYSSSFQW
LHFFVSSLPGGLCMVITVSTIAYKFMGWRTDRRFKEALDRCTVTLAKGACMGIATYITIKLIGIAHDNKWSYLDSGWGQW
FMLEIIVGFMLPLVLMTYGVHTRKMGVIRLGAFATVFGVVLNRVNTAIITFNWKLPEREIPPWREVVISLTIFAIYIVVY
RFILYRLPILYKWRQQEEEVTVTEAVTAPAAGHREASPVAVASYSKMTKLD
>Mature_451_residues
MKNMIKHGLAVVDSPYDESGKKRTVVQKLLLGLSPSQYLLQAFRNPFNWILAAIFAIGIPILLGRFVFGLAWATSGSNDY
PWGLFLGFGLFAMVPLSSSGFQLGTACEIFGRHDLEPIERLALLNGLLGYFFAVVYLLADLGQPWRLPYPMVVSFGPAAV
LFLVAWHVATYLSVQVAEVSTAFFEWIGFPAGKRGVRRITLGLTVSGIILSTLHQGALGALFTYAPGKVHPLWYSSSFQW
LHFFVSSLPGGLCMVITVSTIAYKFMGWRTDRRFKEALDRCTVTLAKGACMGIATYITIKLIGIAHDNKWSYLDSGWGQW
FMLEIIVGFMLPLVLMTYGVHTRKMGVIRLGAFATVFGVVLNRVNTAIITFNWKLPEREIPPWREVVISLTIFAIYIVVY
RFILYRLPILYKWRQQEEEVTVTEAVTAPAAGHREASPVAVASYSKMTKLD

Specific function: HMWC (high-molecular-weight cytochrome c), ORF2, ORF3, ORF4, ORF5 and ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfates [H]

COG id: COG5557

COG function: function code C; Polysulphide reductase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI2367183, Length=373, Percent_Identity=26.0053619302949, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 50377; Mature: 50377

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMIKHGLAVVDSPYDESGKKRTVVQKLLLGLSPSQYLLQAFRNPFNWILAAIFAIGIP
CCCHHHHCHHEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
ILLGRFVFGLAWATSGSNDYPWGLFLGFGLFAMVPLSSSGFQLGTACEIFGRHDLEPIER
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHH
LALLNGLLGYFFAVVYLLADLGQPWRLPYPMVVSFGPAAVLFLVAWHVATYLSVQVAEVS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
TAFFEWIGFPAGKRGVRRITLGLTVSGIILSTLHQGALGALFTYAPGKVHPLWYSSSFQW
HHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
LHFFVSSLPGGLCMVITVSTIAYKFMGWRTDRRFKEALDRCTVTLAKGACMGIATYITIK
HHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEHEE
LIGIAHDNKWSYLDSGWGQWFMLEIIVGFMLPLVLMTYGVHTRKMGVIRLGAFATVFGVV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEHHHHHHHHHHH
LNRVNTAIITFNWKLPEREIPPWREVVISLTIFAIYIVVYRFILYRLPILYKWRQQEEEV
HHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCE
TVTEAVTAPAAGHREASPVAVASYSKMTKLD
EHHHHHCCCCCCCCCCCCEEEEHHHHHHCCC
>Mature Secondary Structure
MKNMIKHGLAVVDSPYDESGKKRTVVQKLLLGLSPSQYLLQAFRNPFNWILAAIFAIGIP
CCCHHHHCHHEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
ILLGRFVFGLAWATSGSNDYPWGLFLGFGLFAMVPLSSSGFQLGTACEIFGRHDLEPIER
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCHHHH
LALLNGLLGYFFAVVYLLADLGQPWRLPYPMVVSFGPAAVLFLVAWHVATYLSVQVAEVS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHH
TAFFEWIGFPAGKRGVRRITLGLTVSGIILSTLHQGALGALFTYAPGKVHPLWYSSSFQW
HHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
LHFFVSSLPGGLCMVITVSTIAYKFMGWRTDRRFKEALDRCTVTLAKGACMGIATYITIK
HHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEHEE
LIGIAHDNKWSYLDSGWGQWFMLEIIVGFMLPLVLMTYGVHTRKMGVIRLGAFATVFGVV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEHHHHHHHHHHH
LNRVNTAIITFNWKLPEREIPPWREVVISLTIFAIYIVVYRFILYRLPILYKWRQQEEEV
HHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCE
TVTEAVTAPAAGHREASPVAVASYSKMTKLD
EHHHHHCCCCCCCCCCCCEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]