Click here to switch to the map view.

The map label for this gene is yagI [H]

Identifier: 197117053

GI number: 197117053

Start: 778587

End: 779369

Strand: Direct

Name: yagI [H]

Synonym: Gbem_0656

Alternate gene names: 197117053

Gene position: 778587-779369 (Clockwise)

Preceding gene: 197117046

Following gene: 308535181

Centisome position: 16.87

GC content: 59.39

Gene sequence:

>783_bases
ATGGCGAAAAAAGAGAAATCCGAGTACATCATCCAGGCCGTTTCGCACGCTCTCGACCTGCTGGAGCAGTTTCACGACGA
AGTGGACGAGCTGGGCGTGACGGAGCTGAGCAAAAGGCTCAAGCTTCATAAGAACAACGTTTTCAGGCTTCTCGCTACCC
TGGAGTCGAGAAACTACATCGAGCAGAACAGGGTCACGGAGAACTACCGGCTGGGGCTCAAGACCCTGGAACTGGGGCAG
ACCTTCATCAAGCAGATGGGGCTTTTAAGGCAGTCCCGCCCGGTGCTGGAGACGCTGGTGAAGGAGTGCAACGAGACTAC
CTACGTGGCGATCCTCAAGGAGTTCCACATCGTCTATCTCGACGTGGTGGAGACCGACCTGACCGTGAGGGTGGTGCCGA
GGGTCGGCGCCCGGCTCCCCGCCTACTGCACAGCGGCAGGGAAGGTCCAGATCGCCTACATGACCGACGAGGAACTGGAG
AACTACCTCCCCACCAAGGAGATGAAGCGCTACACCCCGAAGACGGTGATCGACCGCGAGGAGCTGAAAAAGCACCTCAA
GGTGATCGCGGACCAGGGTTATGCCATAGACGACGAGGAAATGGATGTCGGCGTGAAATGCGTCGGCGCCCCGATCCGCG
ACTACACCCGCAGGATCATCGGCGCGGTGAGCATCTCCGGCCCCTCGATGCGCTTCACCGACGAGCGCCTGGAGAAGGAG
CTGATCCCGCTGGTGATTCGCTCCGGCGAGGAGATCTCCCACAAGCTCGGCTACCACAAATAA

Upstream 100 bases:

>100_bases
TAAAAGCGTTTATCAAGCTATTTATTTCCTTGACAGCTTTTATTAAATGTCCTAGTTAATACTGTTTGAAATTTTTTCCA
TAACAGAATAGGTGCAGCCA

Downstream 100 bases:

>100_bases
AGAAAAGGCCCCGTTCGCGGGGCCTTTTCTTTTTCAGTTCTACGGTTCGGCGGGGGGGGGCTGCCCGGCCGCGATCTCCT
CGCGCCAGACCCTTAGCACC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAKKEKSEYIIQAVSHALDLLEQFHDEVDELGVTELSKRLKLHKNNVFRLLATLESRNYIEQNRVTENYRLGLKTLELGQ
TFIKQMGLLRQSRPVLETLVKECNETTYVAILKEFHIVYLDVVETDLTVRVVPRVGARLPAYCTAAGKVQIAYMTDEELE
NYLPTKEMKRYTPKTVIDREELKKHLKVIADQGYAIDDEEMDVGVKCVGAPIRDYTRRIIGAVSISGPSMRFTDERLEKE
LIPLVIRSGEEISHKLGYHK

Sequences:

>Translated_260_residues
MAKKEKSEYIIQAVSHALDLLEQFHDEVDELGVTELSKRLKLHKNNVFRLLATLESRNYIEQNRVTENYRLGLKTLELGQ
TFIKQMGLLRQSRPVLETLVKECNETTYVAILKEFHIVYLDVVETDLTVRVVPRVGARLPAYCTAAGKVQIAYMTDEELE
NYLPTKEMKRYTPKTVIDREELKKHLKVIADQGYAIDDEEMDVGVKCVGAPIRDYTRRIIGAVSISGPSMRFTDERLEKE
LIPLVIRSGEEISHKLGYHK
>Mature_259_residues
AKKEKSEYIIQAVSHALDLLEQFHDEVDELGVTELSKRLKLHKNNVFRLLATLESRNYIEQNRVTENYRLGLKTLELGQT
FIKQMGLLRQSRPVLETLVKECNETTYVAILKEFHIVYLDVVETDLTVRVVPRVGARLPAYCTAAGKVQIAYMTDEELEN
YLPTKEMKRYTPKTVIDREELKKHLKVIADQGYAIDDEEMDVGVKCVGAPIRDYTRRIIGAVSISGPSMRFTDERLEKEL
IPLVIRSGEEISHKLGYHK

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=249, Percent_Identity=34.9397590361446, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1788131, Length=249, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI87082359, Length=249, Percent_Identity=29.3172690763052, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786715, Length=246, Percent_Identity=29.2682926829268, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1789999, Length=261, Percent_Identity=27.2030651340996, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790752, Length=225, Percent_Identity=25.3333333333333, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788581, Length=248, Percent_Identity=22.9838709677419, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29909; Mature: 29778

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKEKSEYIIQAVSHALDLLEQFHDEVDELGVTELSKRLKLHKNNVFRLLATLESRNYI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EQNRVTENYRLGLKTLELGQTFIKQMGLLRQSRPVLETLVKECNETTYVAILKEFHIVYL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHEEEE
DVVETDLTVRVVPRVGARLPAYCTAAGKVQIAYMTDEELENYLPTKEMKRYTPKTVIDRE
EEECCCCEEEEECHHCCCCCHHEECCCCEEEEEECHHHHHHCCCHHHHHHCCCHHHCCHH
ELKKHLKVIADQGYAIDDEEMDVGVKCVGAPIRDYTRRIIGAVSISGPSMRFTDERLEKE
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHEECCCCCCCCCHHHHHHH
LIPLVIRSGEEISHKLGYHK
HHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure 
AKKEKSEYIIQAVSHALDLLEQFHDEVDELGVTELSKRLKLHKNNVFRLLATLESRNYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EQNRVTENYRLGLKTLELGQTFIKQMGLLRQSRPVLETLVKECNETTYVAILKEFHIVYL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHEEEE
DVVETDLTVRVVPRVGARLPAYCTAAGKVQIAYMTDEELENYLPTKEMKRYTPKTVIDRE
EEECCCCEEEEECHHCCCCCHHEECCCCEEEEEECHHHHHHCCCHHHHHHCCCHHHCCHH
ELKKHLKVIADQGYAIDDEEMDVGVKCVGAPIRDYTRRIIGAVSISGPSMRFTDERLEKE
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHEECCCCCCCCCHHHHHHH
LIPLVIRSGEEISHKLGYHK
HHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]