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The map label for this gene is angR [H]

Identifier: 197116940

GI number: 197116940

Start: 654527

End: 656131

Strand: Reverse

Name: angR [H]

Synonym: Gbem_0542

Alternate gene names: 197116940

Gene position: 656131-654527 (Counterclockwise)

Preceding gene: 197116941

Following gene: 197116939

Centisome position: 14.22

GC content: 63.8

Gene sequence:

>1605_bases
ATGTACCTACTGCAACGATTACTCACCCGAAGCGCCACCTCCTTCCCCGACAAAACCGCGGTCTCCTTCCGCAACGAGGA
GCTCTCCTACGCCGAGCTCGAGGCGGCGAGCAACCAGCTAAGCGCAGCCCTGAAGGCGCACGGCGTGCAAAGAGGAGACA
GGGTCGGAATCCTCATGAACAAATCGCTTGAATCCATCGTCTCCGTCTTTGGCATCCTGAAGGCGGGCGCGATCTACGTC
CCACTGGACCCAGGGGCGCCGGCTAGCAGGCAGGCCTCCATCATCAGGCACTGCGGCATCGAGATCGTCCTCGCCGCGCC
GCAACTGCTGGAGCAACTCCTGGCCGAGGCAGGGGAGGCCCCGCCGCTGCGCGCAGCCATCGTCACCGGCTCCCCGGCGG
TGACACTCCCCCACCCGGCCGGCAGCATGAGCTGCAGCGGCTGGGACGAGATCCTAGGCGAGAGCTGCGAGTGCCCTGCG
GACGACGGGCTTTGCGGCGCTGCTCCCGCCTATATCCTGCACACCTCAGGCTCCACAGGCTCACCCAAGGGGGTGGTGAT
CTCCCACCTGAACGCCCTCACCTTCGTTGAGATGGCTGTCCGCTTCTTCGAGATATCGCCGCAGGACCGTCTGGCCAATC
ACGCGCCGCTGCATTTCGACCTCTCCATCTTCGACATTTTCTGCGCCGCCAGAAGCGCTGCAACGATGGTGCTGATTCCC
GATGCGCTCTCGGCCTTCCCGGTGCGCCTGGCGGATTTCATGCAGAGCGAGGGAGTCACGGTGTGGAACTCGGTGGCGTC
GCTCCTTACCAAGCTCGCGGACCAGGGGGCGCTGGACCGGCTCACCCTGGACAGGCTGCGCCTGGTCCACTTCTCCGGGG
ACCTGATGCCGGTCAAATACCTGAAGATCCTGAAGCAGTGCATGCCGGCTGCAGTCTTTTACAACATCTACGGCCAGACC
GAGGCCAACTCCTCCCTCTATTTCAGGGTCCCGGATGTCGTGGAGGAAGCGGCCTGGAAGATCCCGATCGGGACCCCCTT
CCCCAATTTCGAGGTGTTCGCTATCGACGAGGTGGGGAACGTGGTGACCGGCGCGGGAGAGGAGGGTGAGCTGCACGTTC
TCAGCTCCACCGTGGCCTTGGGCTACTGGAACGACTGCGGCAGAACTCAGGCGCAGTTCACCCCGGATCCGCGCAACCCC
TCCGCCCACGCCAGGGTGTACAAGACCGGGGACATGGCGCGCCTGGACGCCGCCGGCAACTTCGTCTTCGCCGGCCGCAA
GGACCATATGGTGAAGAGCAAGGGGTTCCGGGTGGAGTTGGGCGAGATCGAAATCGTACTGAACAGCCACCCCGACATCC
GGCAGGCGGTTGCGGTGGCCATCCCCGACGACCTCGCCGGAAGCAGGATCGTCGCTTACGTATGCCTGCGCGAAGGGGTC
GAACTCGAGCCCCAAACACTGGTCGTTCATTGCAGCGACCATCTCCCGAAATACATGGTGCCGGAGCAGATCAGGTACCT
CCCCTCCCTGCCGGTGACCTCCAACAGCAAGATAGACCGCAAGGCCTTGGTGCAGGCGTTTCTCGACGGGCCTGCTGAAC
CATAA

Upstream 100 bases:

>100_bases
CGCCATCGCCGGGAAGATCTACTCCGGCACCTCCGAGATCCAGCGCAACATCATCGCCACCTTCCTGGGGCTCTAGCGGA
GGGGGCCCAGCGAGGCATCC

Downstream 100 bases:

>100_bases
GGCGGGGGCACCGCCCCGCCTCCCCTCCTCTCTCCCTCCTGAGCCCCGCCCGGCCGATTCAAAGCAGATAATTAAAGCAC
AATTAATTGATTTCACGGCG

Product: AMP-forming acyl-CoA synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MYLLQRLLTRSATSFPDKTAVSFRNEELSYAELEAASNQLSAALKAHGVQRGDRVGILMNKSLESIVSVFGILKAGAIYV
PLDPGAPASRQASIIRHCGIEIVLAAPQLLEQLLAEAGEAPPLRAAIVTGSPAVTLPHPAGSMSCSGWDEILGESCECPA
DDGLCGAAPAYILHTSGSTGSPKGVVISHLNALTFVEMAVRFFEISPQDRLANHAPLHFDLSIFDIFCAARSAATMVLIP
DALSAFPVRLADFMQSEGVTVWNSVASLLTKLADQGALDRLTLDRLRLVHFSGDLMPVKYLKILKQCMPAAVFYNIYGQT
EANSSLYFRVPDVVEEAAWKIPIGTPFPNFEVFAIDEVGNVVTGAGEEGELHVLSSTVALGYWNDCGRTQAQFTPDPRNP
SAHARVYKTGDMARLDAAGNFVFAGRKDHMVKSKGFRVELGEIEIVLNSHPDIRQAVAVAIPDDLAGSRIVAYVCLREGV
ELEPQTLVVHCSDHLPKYMVPEQIRYLPSLPVTSNSKIDRKALVQAFLDGPAEP

Sequences:

>Translated_534_residues
MYLLQRLLTRSATSFPDKTAVSFRNEELSYAELEAASNQLSAALKAHGVQRGDRVGILMNKSLESIVSVFGILKAGAIYV
PLDPGAPASRQASIIRHCGIEIVLAAPQLLEQLLAEAGEAPPLRAAIVTGSPAVTLPHPAGSMSCSGWDEILGESCECPA
DDGLCGAAPAYILHTSGSTGSPKGVVISHLNALTFVEMAVRFFEISPQDRLANHAPLHFDLSIFDIFCAARSAATMVLIP
DALSAFPVRLADFMQSEGVTVWNSVASLLTKLADQGALDRLTLDRLRLVHFSGDLMPVKYLKILKQCMPAAVFYNIYGQT
EANSSLYFRVPDVVEEAAWKIPIGTPFPNFEVFAIDEVGNVVTGAGEEGELHVLSSTVALGYWNDCGRTQAQFTPDPRNP
SAHARVYKTGDMARLDAAGNFVFAGRKDHMVKSKGFRVELGEIEIVLNSHPDIRQAVAVAIPDDLAGSRIVAYVCLREGV
ELEPQTLVVHCSDHLPKYMVPEQIRYLPSLPVTSNSKIDRKALVQAFLDGPAEP
>Mature_534_residues
MYLLQRLLTRSATSFPDKTAVSFRNEELSYAELEAASNQLSAALKAHGVQRGDRVGILMNKSLESIVSVFGILKAGAIYV
PLDPGAPASRQASIIRHCGIEIVLAAPQLLEQLLAEAGEAPPLRAAIVTGSPAVTLPHPAGSMSCSGWDEILGESCECPA
DDGLCGAAPAYILHTSGSTGSPKGVVISHLNALTFVEMAVRFFEISPQDRLANHAPLHFDLSIFDIFCAARSAATMVLIP
DALSAFPVRLADFMQSEGVTVWNSVASLLTKLADQGALDRLTLDRLRLVHFSGDLMPVKYLKILKQCMPAAVFYNIYGQT
EANSSLYFRVPDVVEEAAWKIPIGTPFPNFEVFAIDEVGNVVTGAGEEGELHVLSSTVALGYWNDCGRTQAQFTPDPRNP
SAHARVYKTGDMARLDAAGNFVFAGRKDHMVKSKGFRVELGEIEIVLNSHPDIRQAVAVAIPDDLAGSRIVAYVCLREGV
ELEPQTLVVHCSDHLPKYMVPEQIRYLPSLPVTSNSKIDRKALVQAFLDGPAEP

Specific function: According to Ref.3:an enzyme involved in the biosynthesis of anguibactin; an iron-binding siderophore [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=561, Percent_Identity=26.9162210338681, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI187761343, Length=561, Percent_Identity=26.9162210338681, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI45580730, Length=560, Percent_Identity=26.0714285714286, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI156151445, Length=547, Percent_Identity=22.1206581352834, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI28416953, Length=543, Percent_Identity=23.0202578268877, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI122937307, Length=511, Percent_Identity=23.4833659491194, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI38505220, Length=530, Percent_Identity=24.3396226415094, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI157311624, Length=528, Percent_Identity=21.969696969697, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI157311622, Length=528, Percent_Identity=21.969696969697, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI58082049, Length=534, Percent_Identity=22.6591760299625, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI42544132, Length=543, Percent_Identity=25.2302025782689, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI227499619, Length=553, Percent_Identity=24.0506329113924, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI13325057, Length=534, Percent_Identity=25.6554307116105, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13236579, Length=512, Percent_Identity=26.3671875, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI115511026, Length=234, Percent_Identity=26.0683760683761, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1786801, Length=551, Percent_Identity=28.6751361161525, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI145693145, Length=536, Percent_Identity=25.9328358208955, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788107, Length=555, Percent_Identity=25.4054054054054, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1790505, Length=561, Percent_Identity=23.5294117647059, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=27.7246653919694, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786810, Length=540, Percent_Identity=23.5185185185185, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1786529, Length=565, Percent_Identity=22.1238938053097, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=554, Percent_Identity=24.187725631769, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=513, Percent_Identity=22.0272904483431, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=551, Percent_Identity=22.5045372050817, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=551, Percent_Identity=22.5045372050817, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=551, Percent_Identity=22.5045372050817, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=509, Percent_Identity=22.5933202357564, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=548, Percent_Identity=22.2627737226277, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI32564422, Length=372, Percent_Identity=22.3118279569892, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32564420, Length=372, Percent_Identity=22.3118279569892, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556356, Length=363, Percent_Identity=25.6198347107438, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=601, Percent_Identity=24.459234608985, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6323182, Length=566, Percent_Identity=24.5583038869258, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319699, Length=365, Percent_Identity=24.3835616438356, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6319264, Length=562, Percent_Identity=24.1992882562278, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648676, Length=534, Percent_Identity=27.1535580524345, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24581924, Length=553, Percent_Identity=24.2314647377938, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24656500, Length=514, Percent_Identity=23.9299610894942, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI18859661, Length=527, Percent_Identity=23.9089184060721, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356947, Length=363, Percent_Identity=23.6914600550964, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=20.8728652751423, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648260, Length=369, Percent_Identity=23.5772357723577, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 57645; Mature: 57645

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLLQRLLTRSATSFPDKTAVSFRNEELSYAELEAASNQLSAALKAHGVQRGDRVGILMN
CHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
KSLESIVSVFGILKAGAIYVPLDPGAPASRQASIIRHCGIEIVLAAPQLLEQLLAEAGEA
CCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCC
PPLRAAIVTGSPAVTLPHPAGSMSCSGWDEILGESCECPADDGLCGAAPAYILHTSGSTG
CCCEEEEEECCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCC
SPKGVVISHLNALTFVEMAVRFFEISPQDRLANHAPLHFDLSIFDIFCAARSAATMVLIP
CCCCCEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEHHHHHHHHHHHCCEEEEEEC
DALSAFPVRLADFMQSEGVTVWNSVASLLTKLADQGALDRLTLDRLRLVHFSGDLMPVKY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCHHHH
LKILKQCMPAAVFYNIYGQTEANSSLYFRVPDVVEEAAWKIPIGTPFPNFEVFAIDEVGN
HHHHHHHCCHHEEEEECCCCCCCCEEEEECHHHHHHCCEECCCCCCCCCEEEEEECCCCC
VVTGAGEEGELHVLSSTVALGYWNDCGRTQAQFTPDPRNPSAHARVYKTGDMARLDAAGN
EEECCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCC
FVFAGRKDHMVKSKGFRVELGEIEIVLNSHPDIRQAVAVAIPDDLAGSRIVAYVCLREGV
EEEECCCCCEECCCCCEEEECEEEEEECCCCCHHHHEEEECCCCCCCCHHHHHHHHHCCC
ELEPQTLVVHCSDHLPKYMVPEQIRYLPSLPVTSNSKIDRKALVQAFLDGPAEP
CCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYLLQRLLTRSATSFPDKTAVSFRNEELSYAELEAASNQLSAALKAHGVQRGDRVGILMN
CHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
KSLESIVSVFGILKAGAIYVPLDPGAPASRQASIIRHCGIEIVLAAPQLLEQLLAEAGEA
CCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCC
PPLRAAIVTGSPAVTLPHPAGSMSCSGWDEILGESCECPADDGLCGAAPAYILHTSGSTG
CCCEEEEEECCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCC
SPKGVVISHLNALTFVEMAVRFFEISPQDRLANHAPLHFDLSIFDIFCAARSAATMVLIP
CCCCCEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEEEEHHHHHHHHHHHCCEEEEEEC
DALSAFPVRLADFMQSEGVTVWNSVASLLTKLADQGALDRLTLDRLRLVHFSGDLMPVKY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEECCCCCHHHH
LKILKQCMPAAVFYNIYGQTEANSSLYFRVPDVVEEAAWKIPIGTPFPNFEVFAIDEVGN
HHHHHHHCCHHEEEEECCCCCCCCEEEEECHHHHHHCCEECCCCCCCCCEEEEEECCCCC
VVTGAGEEGELHVLSSTVALGYWNDCGRTQAQFTPDPRNPSAHARVYKTGDMARLDAAGN
EEECCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCC
FVFAGRKDHMVKSKGFRVELGEIEIVLNSHPDIRQAVAVAIPDDLAGSRIVAYVCLREGV
EEEECCCCCEECCCCCEEEECEEEEEECCCCCHHHHEEEECCCCCCCCHHHHHHHHHCCC
ELEPQTLVVHCSDHLPKYMVPEQIRYLPSLPVTSNSKIDRKALVQAFLDGPAEP
CCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2311935; 8335354 [H]