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The map label for this gene is lexA-2 [H]

Identifier: 197116899

GI number: 197116899

Start: 607937

End: 608686

Strand: Reverse

Name: lexA-2 [H]

Synonym: Gbem_0501

Alternate gene names: 197116899

Gene position: 608686-607937 (Counterclockwise)

Preceding gene: 197116918

Following gene: 197116898

Centisome position: 13.19

GC content: 59.87

Gene sequence:

>750_bases
ATGACGCCTAAACAGAAGAGGGTGCTTGATTTCATTTTGTCATTCGCGGAGCGGCACGGGTTCCAGCCCTCGCAACAGGA
GATCGCCTCTGGATGCGGCTTCGGCTCGCTGGGGACGGTGCAGCATTACTTGCGCGCTCTGGAGAAAGACGGACACCTGA
CTCGCCAGTGGAACGCGAAACGAGGTCTGCAACTGGCAAATCCCCCCTGTCCCCCCTTCGCAAAGGGGGGGACGCAAGTT
CTCGCGCAACGCTTCGTGGGCAAATACTCTCCAGGTTCTCCGGAATCCCAGGAACTGCACTTTGCTGAGGGGGGAAATCC
ACAAACGCCTGCTGTCGCACCGATCATAGGAATGGAGCTGCCGCTGGTGGGGATCGTGGCGGCGGGACGGCCGGTGCAGG
CTTTCCAACTGGCGGACGCCATCGAGGTCCCTTCTGCGATGGCGGGACCGGGCAACGTGGTGTACGAGGTGCGCGGCGAT
TCGATGGTGGAGATGGGGATCATGGACGGCGATTACGTGGCTGTGCACCCGCAGGCTGTGGCGGAGACGGGCCAGACGGT
GATCGCGGAGGTGAACGGTTCGATCACGATCAAGAAGTACGTCCGGAAGGGAAATTCGATTCAGTTGCAGCCGGCGAACT
CGGCGATGAGCCCCATCATGGTGACGGAAGACGACGAGTTTCATATCAGGGGCGTGCTGGTGGGCTCTATGAGGTTTTAT
AAGAAGTGGACCGGGAAATCCTCCACATAA

Upstream 100 bases:

>100_bases
TCCTCCTCCTCTCTCCTTCTTCCTCACCCTTTCTCCCTCTCTTGACTTACGACAAACTTGCGAGTATTCTCGCAACCCTG
ATTCGGGGAGAGGGGGACAG

Downstream 100 bases:

>100_bases
CTGTTCCCGCTTTTCCCATCGCGCTGGCCCGGGTCGTCGACTCGAACCTGCGCGGACGGCCGGTGGCGGTGGCTCCGCTC
AACTCGGAACGGGCGCTGCT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTPKQKRVLDFILSFAERHGFQPSQQEIASGCGFGSLGTVQHYLRALEKDGHLTRQWNAKRGLQLANPPCPPFAKGGTQV
LAQRFVGKYSPGSPESQELHFAEGGNPQTPAVAPIIGMELPLVGIVAAGRPVQAFQLADAIEVPSAMAGPGNVVYEVRGD
SMVEMGIMDGDYVAVHPQAVAETGQTVIAEVNGSITIKKYVRKGNSIQLQPANSAMSPIMVTEDDEFHIRGVLVGSMRFY
KKWTGKSST

Sequences:

>Translated_249_residues
MTPKQKRVLDFILSFAERHGFQPSQQEIASGCGFGSLGTVQHYLRALEKDGHLTRQWNAKRGLQLANPPCPPFAKGGTQV
LAQRFVGKYSPGSPESQELHFAEGGNPQTPAVAPIIGMELPLVGIVAAGRPVQAFQLADAIEVPSAMAGPGNVVYEVRGD
SMVEMGIMDGDYVAVHPQAVAETGQTVIAEVNGSITIKKYVRKGNSIQLQPANSAMSPIMVTEDDEFHIRGVLVGSMRFY
KKWTGKSST
>Mature_248_residues
TPKQKRVLDFILSFAERHGFQPSQQEIASGCGFGSLGTVQHYLRALEKDGHLTRQWNAKRGLQLANPPCPPFAKGGTQVL
AQRFVGKYSPGSPESQELHFAEGGNPQTPAVAPIIGMELPLVGIVAAGRPVQAFQLADAIEVPSAMAGPGNVVYEVRGDS
MVEMGIMDGDYVAVHPQAVAETGQTVIAEVNGSITIKKYVRKGNSIQLQPANSAMSPIMVTEDDEFHIRGVLVGSMRFYK
KWTGKSST

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=241, Percent_Identity=30.2904564315353, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787431, Length=80, Percent_Identity=40, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 26791; Mature: 26660

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKQKRVLDFILSFAERHGFQPSQQEIASGCGFGSLGTVQHYLRALEKDGHLTRQWNAK
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
RGLQLANPPCPPFAKGGTQVLAQRFVGKYSPGSPESQELHFAEGGNPQTPAVAPIIGMEL
CCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCC
PLVGIVAAGRPVQAFQLADAIEVPSAMAGPGNVVYEVRGDSMVEMGIMDGDYVAVHPQAV
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCEEEECCHHH
AETGQTVIAEVNGSITIKKYVRKGNSIQLQPANSAMSPIMVTEDDEFHIRGVLVGSMRFY
HHCCCEEEEECCCCEEEEHHHHCCCCEEEEECCCCCCCEEEECCCCEEEEEEEECCHHHH
KKWTGKSST
HHHCCCCCC
>Mature Secondary Structure 
TPKQKRVLDFILSFAERHGFQPSQQEIASGCGFGSLGTVQHYLRALEKDGHLTRQWNAK
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
RGLQLANPPCPPFAKGGTQVLAQRFVGKYSPGSPESQELHFAEGGNPQTPAVAPIIGMEL
CCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCC
PLVGIVAAGRPVQAFQLADAIEVPSAMAGPGNVVYEVRGDSMVEMGIMDGDYVAVHPQAV
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCEEEECCHHH
AETGQTVIAEVNGSITIKKYVRKGNSIQLQPANSAMSPIMVTEDDEFHIRGVLVGSMRFY
HHCCCEEEEECCCCEEEEHHHHCCCCEEEEECCCCCCCEEEECCCCEEEEEEEECCHHHH
KKWTGKSST
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA