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The map label for this gene is ddl

Identifier: 197116892

GI number: 197116892

Start: 596677

End: 597594

Strand: Direct

Name: ddl

Synonym: Gbem_0494

Alternate gene names: 197116892

Gene position: 596677-597594 (Clockwise)

Preceding gene: 197116891

Following gene: 197116893

Centisome position: 12.93

GC content: 64.71

Gene sequence:

>918_bases
ATGACGGTAGAGGAGCTGAAAAAGAAGAAGATCGCGGTGCTGATGGGTGGACTTTCCGCCGAGCGCGAAGTGTCCCTCAA
CAGCGGCAAGGCGGTCCTTGCCTCCCTGGTCAAGCAGGGTTTCCGGGCGGTGGGGATCGACGTCGGTCGCGACCTGCCGC
AGCGGCTGGCCGAGGAGCAGGTGGAATTGGCCTTCATCGCGCTGCACGGCCGCTTCGGCGAGGATGGCTCGGTCCAGGGA
CTCCTGGAACTGATGGGCATCCCCTATACCGGCTCCGGGGTACTTGCCAGCGCCCTCGCCATCGACAAAATAGCTTCCAA
GGTCATCTTCGCCTCCGCCGGGCTGAAGCTCGCTCCCTACCAGGTGCTGCGCCGGGGCGAGGAGCTCAAGCTAGCCAACC
CGCTTCCGGTCGTGGTGAAGCCTTCCCGCGAAGGCTCCTCCGTCGGAGTCGGCATCGTGCGCGACCCTTCCCGGATGCAG
GCGGCGCTCGACGAGGCGTTCCGCTACGACTCCGAGATCCTCATCGAGGGTTTCATCGACGGTCGCGAAGTCCAGGTCGG
CATACTGAACGGCAAGGCGCTGGGCGCCATCGAGATCATCCCCAAGGGGGAATTCTACGACTACGAGGCGAAGTACACCG
ACGGCGGCGCGCAGCACATCCTGCCGGCGCGCCTCCCCGAGGCGGTCTACGCCGAGGTGCTTCGTCAGGGTGAGAAGGCG
CACGCGGCGCTGGGGTGCGACTGCTACAGCAGGGTCGACTTCCTGGTCACCGAAACGGGCGACTGCTTCCTCTTGGAGGT
GAACACCCTCCCCGGGATGACGGACTTAAGCCTTCTTCCCGAGATAGCGGGGGGAGCCGGAATCGCTTTCGGCGAGTTGG
TACTGCGCATACTGCAGGCGGCATCGCTGAAGATCTAG

Upstream 100 bases:

>100_bases
GGGGGGATCAAGAGCGTCTTTCCCCGGAAGAACCTTTGAAAAAGGGGGGAGGGGCGTTGGCGGATGGTTCTTGTAATAGA
TGAACCGAAAGGATTGGGCG

Downstream 100 bases:

>100_bases
CTAGCGGCACGAGTAACCTGTGATGTTATTCAATGCTTCCATGACTCAACTTTTTTGACGGTAAAAAAATGCGCGATCTT
CATGCCAAAAAACAGCGGGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTVEELKKKKIAVLMGGLSAEREVSLNSGKAVLASLVKQGFRAVGIDVGRDLPQRLAEEQVELAFIALHGRFGEDGSVQG
LLELMGIPYTGSGVLASALAIDKIASKVIFASAGLKLAPYQVLRRGEELKLANPLPVVVKPSREGSSVGVGIVRDPSRMQ
AALDEAFRYDSEILIEGFIDGREVQVGILNGKALGAIEIIPKGEFYDYEAKYTDGGAQHILPARLPEAVYAEVLRQGEKA
HAALGCDCYSRVDFLVTETGDCFLLEVNTLPGMTDLSLLPEIAGGAGIAFGELVLRILQAASLKI

Sequences:

>Translated_305_residues
MTVEELKKKKIAVLMGGLSAEREVSLNSGKAVLASLVKQGFRAVGIDVGRDLPQRLAEEQVELAFIALHGRFGEDGSVQG
LLELMGIPYTGSGVLASALAIDKIASKVIFASAGLKLAPYQVLRRGEELKLANPLPVVVKPSREGSSVGVGIVRDPSRMQ
AALDEAFRYDSEILIEGFIDGREVQVGILNGKALGAIEIIPKGEFYDYEAKYTDGGAQHILPARLPEAVYAEVLRQGEKA
HAALGCDCYSRVDFLVTETGDCFLLEVNTLPGMTDLSLLPEIAGGAGIAFGELVLRILQAASLKI
>Mature_304_residues
TVEELKKKKIAVLMGGLSAEREVSLNSGKAVLASLVKQGFRAVGIDVGRDLPQRLAEEQVELAFIALHGRFGEDGSVQGL
LELMGIPYTGSGVLASALAIDKIASKVIFASAGLKLAPYQVLRRGEELKLANPLPVVVKPSREGSSVGVGIVRDPSRMQA
ALDEAFRYDSEILIEGFIDGREVQVGILNGKALGAIEIIPKGEFYDYEAKYTDGGAQHILPARLPEAVYAEVLRQGEKAH
AALGCDCYSRVDFLVTETGDCFLLEVNTLPGMTDLSLLPEIAGGAGIAFGELVLRILQAASLKI

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=49.0066225165563, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=31.7142857142857, Blast_Score=177, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_GEOBB (B5EBQ4)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137319.1
- ProteinModelPortal:   B5EBQ4
- SMR:   B5EBQ4
- GeneID:   6783323
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0494
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- ProtClustDB:   CLSK829085
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32505; Mature: 32374

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEELKKKKIAVLMGGLSAEREVSLNSGKAVLASLVKQGFRAVGIDVGRDLPQRLAEEQ
CCHHHHHHHHEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHEECCHHCCHHHHHHHHHH
VELAFIALHGRFGEDGSVQGLLELMGIPYTGSGVLASALAIDKIASKVIFASAGLKLAPY
HEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QVLRRGEELKLANPLPVVVKPSREGSSVGVGIVRDPSRMQAALDEAFRYDSEILIEGFID
HHHHCCCCEEECCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCCEEEEECCC
GREVQVGILNGKALGAIEIIPKGEFYDYEAKYTDGGAQHILPARLPEAVYAEVLRQGEKA
CCEEEEEEECCCCEEEEEEEECCCEECCCCEECCCCCCCCCHHCCCHHHHHHHHHCCCHH
HAALGCDCYSRVDFLVTETGDCFLLEVNTLPGMTDLSLLPEIAGGAGIAFGELVLRILQA
HHEECCCHHHCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ASLKI
HHCCC
>Mature Secondary Structure 
TVEELKKKKIAVLMGGLSAEREVSLNSGKAVLASLVKQGFRAVGIDVGRDLPQRLAEEQ
CHHHHHHHHEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHEECCHHCCHHHHHHHHHH
VELAFIALHGRFGEDGSVQGLLELMGIPYTGSGVLASALAIDKIASKVIFASAGLKLAPY
HEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QVLRRGEELKLANPLPVVVKPSREGSSVGVGIVRDPSRMQAALDEAFRYDSEILIEGFID
HHHHCCCCEEECCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCCEEEEECCC
GREVQVGILNGKALGAIEIIPKGEFYDYEAKYTDGGAQHILPARLPEAVYAEVLRQGEKA
CCEEEEEEECCCCEEEEEEEECCCEECCCCEECCCCCCCCCHHCCCHHHHHHHHHCCCHH
HAALGCDCYSRVDFLVTETGDCFLLEVNTLPGMTDLSLLPEIAGGAGIAFGELVLRILQA
HHEECCCHHHCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ASLKI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA