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The map label for this gene is uraA [H]

Identifier: 197116862

GI number: 197116862

Start: 557879

End: 559156

Strand: Direct

Name: uraA [H]

Synonym: Gbem_0464

Alternate gene names: 197116862

Gene position: 557879-559156 (Clockwise)

Preceding gene: 197116861

Following gene: 197116863

Centisome position: 12.09

GC content: 65.41

Gene sequence:

>1278_bases
ATGTCACAAGCAAAAGAACCGGTATGGCGCCAGGCGCTTTCCGGAGCGCAGATCCTTTTTGTCGCGTTCGGGGCACTGGT
GCTGGTGCCGATTCTCACCGGGCTCAATCCGAGCATGGCGCTTTTGGGCGCCGGCGTGGGCACCCTCATCTTCCAGCTCT
GCACCAAGCGCGAGGTCCCCATCTTCTTAGGCTCCTCCTTCGCCTTCATAGCGCCCATCGTCTACAGCGTCCAGACCTGG
GGGATGCCGGCGACCTTGGGCGGGCTCTTCGCCGCGGGATGGCTCTACCTCGCGCTCTCCCTGGTGATCTACCTGCGCGG
CGCGGATTTCATCCACCGCATCATGCCTCCCGTGGTGGTGGGCCCCATCATCATGGTCATCGGCCTGGGACTTGCCGGCG
TCGCGGTCAACATGGCGATGGGCAAAACCGGAGACGGCAAGGGTGTGCTGGTCGATTACGAAACCGCCATCCTGGTGGCG
GCGGTCTCGCTTGCCACGACCGTCGCGGTGGCCGTGCGCGCCAAGGGGATCTTCCGGCTCTTGCCGGTACTCTCCGGCGT
CGCCGTCGGCTACGTCCTCTCCATCTTCCTGGGGCTGGTCGATTTCACCAAGATCGCCGCCGCCCCCTGGATCGAGGTGC
CGACCTTCGTGACCCCGCAGTTCAACTGGGCGGCTGTCCTGTTCATGATCCCGGTGGCGCTTGCCCCGGCCATCGAGCAT
GTCGGCGACGTCGTCGCCATCGGGGCTGTGACCGGCAAGGATTACACCGTGAAGCCGGGCCTGCACCGCACCATGCTGGG
GGACGGGCTCGCCGTCTGCACGGCCGCGCTGATCGGCGGCCCACCCGTCACCACCTATGCCGAGGTGACCGGGGCGGTGA
TGATCACCCGTTGCTACAACCCGGTCATCATGACCTGGGCCGCAGGCTTCGCCATCGTCATGGCCTTCTTCGGCAAGTTC
AACGCCATCCTGCAGTCGATACCGGTGCCGGTCATGGGGGGGATCATGATGCTCCTCTTCGGCTCCATCGCATCGGTCGG
TCTCAACACCCTGATCCACGCCCAGGTCGACATGCACCGCCCGCGCAACCTGGTCATCGTCTCGCTGGTTCTGGTCTTCG
GCATCGGCGGCCTCAGCCTCAACGTTGCCGGGCAGCACCTGCACGGCGTCTCGCTCTGCGGCATCGCGGCAATCCTGCTC
AACCTCGTCCTGCCCAAAGGCGAGGCACCACCGATGGGAGTTGAGACGGACGATGCGGAGCCGGAGACGGTCCCCTGA

Upstream 100 bases:

>100_bases
AGACGCCGGCGACAAGATCTTCGGCACCAAGTAACGCACCCGGCTGCAACCAAAGCGGGGGCGTTCCCTCCCGTATCACT
TTCCTAGCGAGGTCAACTGC

Downstream 100 bases:

>100_bases
TTTCATTCATCCTGGCGCGCCGGCCTCTGCCGCCGGCGCGCCAGCTTTTCCCCTGTACCGCCCCTTCCCCGTCGCCTCCC
CAACCCGTGTAGCGTGCCCC

Product: uracil transporter

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSQAKEPVWRQALSGAQILFVAFGALVLVPILTGLNPSMALLGAGVGTLIFQLCTKREVPIFLGSSFAFIAPIVYSVQTW
GMPATLGGLFAAGWLYLALSLVIYLRGADFIHRIMPPVVVGPIIMVIGLGLAGVAVNMAMGKTGDGKGVLVDYETAILVA
AVSLATTVAVAVRAKGIFRLLPVLSGVAVGYVLSIFLGLVDFTKIAAAPWIEVPTFVTPQFNWAAVLFMIPVALAPAIEH
VGDVVAIGAVTGKDYTVKPGLHRTMLGDGLAVCTAALIGGPPVTTYAEVTGAVMITRCYNPVIMTWAAGFAIVMAFFGKF
NAILQSIPVPVMGGIMMLLFGSIASVGLNTLIHAQVDMHRPRNLVIVSLVLVFGIGGLSLNVAGQHLHGVSLCGIAAILL
NLVLPKGEAPPMGVETDDAEPETVP

Sequences:

>Translated_425_residues
MSQAKEPVWRQALSGAQILFVAFGALVLVPILTGLNPSMALLGAGVGTLIFQLCTKREVPIFLGSSFAFIAPIVYSVQTW
GMPATLGGLFAAGWLYLALSLVIYLRGADFIHRIMPPVVVGPIIMVIGLGLAGVAVNMAMGKTGDGKGVLVDYETAILVA
AVSLATTVAVAVRAKGIFRLLPVLSGVAVGYVLSIFLGLVDFTKIAAAPWIEVPTFVTPQFNWAAVLFMIPVALAPAIEH
VGDVVAIGAVTGKDYTVKPGLHRTMLGDGLAVCTAALIGGPPVTTYAEVTGAVMITRCYNPVIMTWAAGFAIVMAFFGKF
NAILQSIPVPVMGGIMMLLFGSIASVGLNTLIHAQVDMHRPRNLVIVSLVLVFGIGGLSLNVAGQHLHGVSLCGIAAILL
NLVLPKGEAPPMGVETDDAEPETVP
>Mature_424_residues
SQAKEPVWRQALSGAQILFVAFGALVLVPILTGLNPSMALLGAGVGTLIFQLCTKREVPIFLGSSFAFIAPIVYSVQTWG
MPATLGGLFAAGWLYLALSLVIYLRGADFIHRIMPPVVVGPIIMVIGLGLAGVAVNMAMGKTGDGKGVLVDYETAILVAA
VSLATTVAVAVRAKGIFRLLPVLSGVAVGYVLSIFLGLVDFTKIAAAPWIEVPTFVTPQFNWAAVLFMIPVALAPAIEHV
GDVVAIGAVTGKDYTVKPGLHRTMLGDGLAVCTAALIGGPPVTTYAEVTGAVMITRCYNPVIMTWAAGFAIVMAFFGKFN
AILQSIPVPVMGGIMMLLFGSIASVGLNTLIHAQVDMHRPRNLVIVSLVLVFGIGGLSLNVAGQHLHGVSLCGIAAILLN
LVLPKGEAPPMGVETDDAEPETVP

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=383, Percent_Identity=25.8485639686684, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI44680143, Length=387, Percent_Identity=25.3229974160207, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI44680148, Length=381, Percent_Identity=22.8346456692913, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI40316845, Length=381, Percent_Identity=22.8346456692913, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788843, Length=407, Percent_Identity=38.5749385749386, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87081818, Length=434, Percent_Identity=34.7926267281106, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87082178, Length=346, Percent_Identity=26.878612716763, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790087, Length=402, Percent_Identity=26.3681592039801, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082181, Length=329, Percent_Identity=25.2279635258359, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17542262, Length=246, Percent_Identity=27.2357723577236, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17558856, Length=203, Percent_Identity=29.5566502463054, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17541904, Length=220, Percent_Identity=23.6363636363636, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356175, Length=291, Percent_Identity=26.4604810996564, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 44231; Mature: 44099

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCC
>Mature Secondary Structure 
SQAKEPVWRQALSGAQILFVAFGALVLVPILTGLNPSMALLGAGVGTLIFQLCTKREVP
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCC
IFLGSSFAFIAPIVYSVQTWGMPATLGGLFAAGWLYLALSLVIYLRGADFIHRIMPPVVV
EEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHH
GPIIMVIGLGLAGVAVNMAMGKTGDGKGVLVDYETAILVAAVSLATTVAVAVRAKGIFRL
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPVLSGVAVGYVLSIFLGLVDFTKIAAAPWIEVPTFVTPQFNWAAVLFMIPVALAPAIEH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
VGDVVAIGAVTGKDYTVKPGLHRTMLGDGLAVCTAALIGGPPVTTYAEVTGAVMITRCYN
HCCEEEEEEECCCCEEECCCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHEEHHCC
PVIMTWAAGFAIVMAFFGKFNAILQSIPVPVMGGIMMLLFGSIASVGLNTLIHAQVDMHR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRNLVIVSLVLVFGIGGLSLNVAGQHLHGVSLCGIAAILLNLVLPKGEAPPMGVETDDAE
CCHHHHHHHHHHHCCCCCEEEECCCHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PETVP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]