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The map label for this gene is bkdF [H]

Identifier: 197116859

GI number: 197116859

Start: 554561

End: 555781

Strand: Direct

Name: bkdF [H]

Synonym: Gbem_0461

Alternate gene names: 197116859

Gene position: 554561-555781 (Clockwise)

Preceding gene: 197116858

Following gene: 308535165

Centisome position: 12.02

GC content: 67.24

Gene sequence:

>1221_bases
ATGTCTATCGATTTCAAACTCCCCGATCTGGGCGAAGGCATCGCCGAGGTGGAACTGCGCCGCTGGCTGGTGGCGGAAGG
GGATGCTGTTGCGGAACACCAGCCGCTGGTCGAGGTGGAGACGGACAAGGCGGTGGTCGAGGTCCCATCCCCGCGCGCCG
GTGTCGTCGCCCGCCTTCACTGCAAGGAGGGGGAGACGGTTCAGGTCGGCGCCACGCTGGTGACTTTCGCCGAGGCGAAG
GAGGCCGCCAAGAAAGAGGAGCCCGAAGGGGAGCGCAGGCCGGCGCAGCGCCCGCCCTCGGTCGGCATCGTCGGCTCGCT
GCCGGAACCGGAGGAGGAGGCAACTCCGGCCGCACCGGCGGGGTTCGAGGGACTGGCGACCCCGATGGTGAGGAAGATGG
CCCGGGAGCGGGGTATCGACCTGAAAAGCGTGCGGGGCACCGGGCCGCGCGGCTGCATCAAGCCCGAGGATCTGGACCAG
GTTCCCCTGGCGGCGCAGAAAGCGAAGCCGGCGCCGCCAGACGGGGAACGGGTGCCGCTCAGAGGCCTGCGGCGTACCAT
CGCCCGGAACGTGCTGGCCTCCCAAAGGACCACCGCCTTCGTCACCAGCATGGAAGAGGTCGACATTACCGACATATGGG
AGATGCGGGGGCGCGAGCAGGGGGAAGTGGAGTCGCGGGGGGCGCACCTGACCTTCCTCCCCTTCTTCATCAAGGCGGTC
CAGCATGCGCTGCGCGAACACCCGCTTTTGAACGGCTCCATCGACGACGAGGCGCAGGAACTGGTGCTGAAAAAGCACTA
CCATTTCGGGATCGCGGTGGACACCCCGGAGGGGCTCATGGTCCCGGTGATCCGGGACGTGGACAAGAAGAGCATCATCG
AGCTGGCGCAGGCGGTCCAGGAACTCGGCCGCAAGGCGCGCGAGCGGAGCATTTCGTTGGAGGAACTGCGCGGCAGCAGT
TTCACCATCACCAACTACGGCCACTTCGGCGGCACCTTCGCCACTCCCATCATCAACTGGCCCGACGTCGCCATCATGGG
CTTTGGGCGCATCGTAGAACGCCCCTGGGTGCACCGGGGCCAGATCGCCATCAGGAAGATCCTGCCGTTGTCGCTCACCT
TCGACCACCGCGCCACCGACGGCGCCGACGCCGCCAGGTTCCTGGGCAAGGTGCTCCGCTACCTCGAGGACCCCGCGCTC
CTCTTCCTGGACAGCGCCTAG

Upstream 100 bases:

>100_bases
AGGCTCTCACCACAGAGGAACACAGAGGAAGGCAAAATCTGAAGGCTTTGTTGTTGCTCTTTGCTCCCTGGTCACGCTTT
TCGGTTTTGAGGGGTTCACT

Downstream 100 bases:

>100_bases
CCCGGGCCGTCGCCGTTTCGGGCCCGGCAGCACCAGCACCAGCGCCCCTCCCAGCCCCCCCCCAGCCCCCCCCAGCCCCT
AGCCCCTAAAGCGGATCACA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSIDFKLPDLGEGIAEVELRRWLVAEGDAVAEHQPLVEVETDKAVVEVPSPRAGVVARLHCKEGETVQVGATLVTFAEAK
EAAKKEEPEGERRPAQRPPSVGIVGSLPEPEEEATPAAPAGFEGLATPMVRKMARERGIDLKSVRGTGPRGCIKPEDLDQ
VPLAAQKAKPAPPDGERVPLRGLRRTIARNVLASQRTTAFVTSMEEVDITDIWEMRGREQGEVESRGAHLTFLPFFIKAV
QHALREHPLLNGSIDDEAQELVLKKHYHFGIAVDTPEGLMVPVIRDVDKKSIIELAQAVQELGRKARERSISLEELRGSS
FTITNYGHFGGTFATPIINWPDVAIMGFGRIVERPWVHRGQIAIRKILPLSLTFDHRATDGADAARFLGKVLRYLEDPAL
LFLDSA

Sequences:

>Translated_406_residues
MSIDFKLPDLGEGIAEVELRRWLVAEGDAVAEHQPLVEVETDKAVVEVPSPRAGVVARLHCKEGETVQVGATLVTFAEAK
EAAKKEEPEGERRPAQRPPSVGIVGSLPEPEEEATPAAPAGFEGLATPMVRKMARERGIDLKSVRGTGPRGCIKPEDLDQ
VPLAAQKAKPAPPDGERVPLRGLRRTIARNVLASQRTTAFVTSMEEVDITDIWEMRGREQGEVESRGAHLTFLPFFIKAV
QHALREHPLLNGSIDDEAQELVLKKHYHFGIAVDTPEGLMVPVIRDVDKKSIIELAQAVQELGRKARERSISLEELRGSS
FTITNYGHFGGTFATPIINWPDVAIMGFGRIVERPWVHRGQIAIRKILPLSLTFDHRATDGADAARFLGKVLRYLEDPAL
LFLDSA
>Mature_405_residues
SIDFKLPDLGEGIAEVELRRWLVAEGDAVAEHQPLVEVETDKAVVEVPSPRAGVVARLHCKEGETVQVGATLVTFAEAKE
AAKKEEPEGERRPAQRPPSVGIVGSLPEPEEEATPAAPAGFEGLATPMVRKMARERGIDLKSVRGTGPRGCIKPEDLDQV
PLAAQKAKPAPPDGERVPLRGLRRTIARNVLASQRTTAFVTSMEEVDITDIWEMRGREQGEVESRGAHLTFLPFFIKAVQ
HALREHPLLNGSIDDEAQELVLKKHYHFGIAVDTPEGLMVPVIRDVDKKSIIELAQAVQELGRKARERSISLEELRGSSF
TITNYGHFGGTFATPIINWPDVAIMGFGRIVERPWVHRGQIAIRKILPLSLTFDHRATDGADAARFLGKVLRYLEDPALL
FLDSA

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=31.712962962963, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI19923748, Length=244, Percent_Identity=38.1147540983607, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=29.0322580645161, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=26.1437908496732, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI203098816, Length=459, Percent_Identity=26.1437908496732, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=32.5, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786946, Length=420, Percent_Identity=33.8095238095238, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1786305, Length=419, Percent_Identity=34.1288782816229, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17537937, Length=426, Percent_Identity=32.8638497652582, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI25146366, Length=404, Percent_Identity=31.6831683168317, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=432, Percent_Identity=28.4722222222222, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=322, Percent_Identity=28.2608695652174, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=408, Percent_Identity=32.3529411764706, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=23.5555555555556, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=32.1678321678322, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=36.6812227074236, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=30.2721088435374, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=30.2721088435374, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44625; Mature: 44493

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDFKLPDLGEGIAEVELRRWLVAEGDAVAEHQPLVEVETDKAVVEVPSPRAGVVARLH
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCEEEECCCEEEECCCCCCCEEEEEE
CKEGETVQVGATLVTFAEAKEAAKKEEPEGERRPAQRPPSVGIVGSLPEPEEEATPAAPA
CCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCCCCCC
GFEGLATPMVRKMARERGIDLKSVRGTGPRGCIKPEDLDQVPLAAQKAKPAPPDGERVPL
CCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCH
RGLRRTIARNVLASQRTTAFVTSMEEVDITDIWEMRGREQGEVESRGAHLTFLPFFIKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCEEEHHHHHHHHH
QHALREHPLLNGSIDDEAQELVLKKHYHFGIAVDTPEGLMVPVIRDVDKKSIIELAQAVQ
HHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCEEHHHHCCCHHHHHHHHHHHH
ELGRKARERSISLEELRGSSFTITNYGHFGGTFATPIINWPDVAIMGFGRIVERPWVHRG
HHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
QIAIRKILPLSLTFDHRATDGADAARFLGKVLRYLEDPALLFLDSA
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
SIDFKLPDLGEGIAEVELRRWLVAEGDAVAEHQPLVEVETDKAVVEVPSPRAGVVARLH
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCEEEECCCEEEECCCCCCCEEEEEE
CKEGETVQVGATLVTFAEAKEAAKKEEPEGERRPAQRPPSVGIVGSLPEPEEEATPAAPA
CCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCHHHCCCCCCC
GFEGLATPMVRKMARERGIDLKSVRGTGPRGCIKPEDLDQVPLAAQKAKPAPPDGERVPL
CCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCH
RGLRRTIARNVLASQRTTAFVTSMEEVDITDIWEMRGREQGEVESRGAHLTFLPFFIKAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCEEEHHHHHHHHH
QHALREHPLLNGSIDDEAQELVLKKHYHFGIAVDTPEGLMVPVIRDVDKKSIIELAQAVQ
HHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEEECCCCCEEHHHHCCCHHHHHHHHHHHH
ELGRKARERSISLEELRGSSFTITNYGHFGGTFATPIINWPDVAIMGFGRIVERPWVHRG
HHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
QIAIRKILPLSLTFDHRATDGADAARFLGKVLRYLEDPALLFLDSA
HHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]