Click here to switch to the map view.

The map label for this gene is gcvH-1 [H]

Identifier: 197116794

GI number: 197116794

Start: 471755

End: 472126

Strand: Direct

Name: gcvH-1 [H]

Synonym: Gbem_0396

Alternate gene names: 197116794

Gene position: 471755-472126 (Clockwise)

Preceding gene: 197116793

Following gene: 197116795

Centisome position: 10.22

GC content: 62.9

Gene sequence:

>372_bases
ATGGCAAAGTTCTTCACCAAGGAGCACGAGTGGGTAGAGATGGACGGGGCGCAGGCGATCGTCGGCATCAGCGAGCACGC
GGCGCACGAACTGGGGGACATCACCTTCGTGGAACTCCCGAAGATCGGCAAGAGCGTGAAGCAGTTCGACACCCTGGCGG
GGATCGAATCGGTCAAGGCCGCAAGCGACATCTACGCGCCGGTTTCCGGCAAGGTGGCCCAGGTGAACGACAAGCTGGAG
GACGCCCCTGAGCTGGTGAACCAGGGCGCCGAGAGCGAAGGGTGGATCTGCAGGCTGGAAGGTGTTGACGCCTCCGAGCT
TGGTGCGCTCCTGGATGCAGCGGCGTACGCCAAGTACCTGGAAGGACTGTAG

Upstream 100 bases:

>100_bases
CCCCCCTCTTGCCATTCCCAGATCAGGCGCGGACCGGCCTGGGCTGCCAATTAACTCTTTATGCGAACGATACGAACCAC
ACAAAACGGAGGATCACCAG

Downstream 100 bases:

>100_bases
GGATGGGATACTGTCCCAACACACCGGACGAGATCCGGGAGATGCTCGCCGTGATCGGCGCGGGTAGCATCGAGGAGCTC
TTCGCTCCCATCCCGGCGGA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MAKFFTKEHEWVEMDGAQAIVGISEHAAHELGDITFVELPKIGKSVKQFDTLAGIESVKAASDIYAPVSGKVAQVNDKLE
DAPELVNQGAESEGWICRLEGVDASELGALLDAAAYAKYLEGL

Sequences:

>Translated_123_residues
MAKFFTKEHEWVEMDGAQAIVGISEHAAHELGDITFVELPKIGKSVKQFDTLAGIESVKAASDIYAPVSGKVAQVNDKLE
DAPELVNQGAESEGWICRLEGVDASELGALLDAAAYAKYLEGL
>Mature_122_residues
AKFFTKEHEWVEMDGAQAIVGISEHAAHELGDITFVELPKIGKSVKQFDTLAGIESVKAASDIYAPVSGKVAQVNDKLED
APELVNQGAESEGWICRLEGVDASELGALLDAAAYAKYLEGL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=38.0165289256198, Blast_Score=103, Evalue=2e-23,
Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=38.0165289256198, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=54.6218487394958, Blast_Score=120, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=43.5897435897436, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=44.4444444444444, Blast_Score=108, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=39.1666666666667, Blast_Score=98, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=44.5378151260504, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13164; Mature: 13033

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFFTKEHEWVEMDGAQAIVGISEHAAHELGDITFVELPKIGKSVKQFDTLAGIESVKA
CCCCCCCCCCEEECCCCEEEECCHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ASDIYAPVSGKVAQVNDKLEDAPELVNQGAESEGWICRLEGVDASELGALLDAAAYAKYL
HHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
EGL
HCC
>Mature Secondary Structure 
AKFFTKEHEWVEMDGAQAIVGISEHAAHELGDITFVELPKIGKSVKQFDTLAGIESVKA
CCCCCCCCCEEECCCCEEEECCHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ASDIYAPVSGKVAQVNDKLEDAPELVNQGAESEGWICRLEGVDASELGALLDAAAYAKYL
HHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
EGL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]