Click here to switch to the map view.

The map label for this gene is hpaG [H]

Identifier: 194738089

GI number: 194738089

Start: 1146892

End: 1148181

Strand: Direct

Name: hpaG [H]

Synonym: SeSA_A1165

Alternate gene names: 194738089

Gene position: 1146892-1148181 (Clockwise)

Preceding gene: 194737332

Following gene: 194734436

Centisome position: 24.35

GC content: 55.66

Gene sequence:

>1290_bases
ATGAAGGGTACTGTTTTCGCCGTTGCGTTAAACCATCGCAGCCAGCTTGATGCCTGGCAAGAGGCTTTCTCTCAGCCTCC
CTATAATGCGCCGCCTAAAACCGCAGTGTGGTTCATCAAGCCGCGTAATACGGTGATTCGTCACGGCGAACCCATTCCTT
ATCCGCAGGGAGAAAAGGTACTGAGCGGCGCGACAGTGGCGCTCATTGTGGGGAAAACCGCCAGTCGGATACGCCCTGAA
GCGGCGGCGGACTATATCGCCGGGTATGCGCTGGCTAACGAGGTCAGCCTGCCGGAAGAGAGCTTTTATCGCCCGGCGAT
TAAAGCGAAATGTCGCGATGGCTTTTGCCCTCTGGGTGAAATGGCGCCGCTGAGTGATGTGGATAACCTCACCATTATCA
CTGAAATCAACGGACGAGAAGCGGACCACTGGAATACTGCCGATTTACAGCGTAGCGCCGCACAGCTGCTTAGCGCGTTA
AGTGAGTTCGCTACGCTTAACCCTGGCGATGCGATCTTACTCGGTACGCCACAGAATCGCGTTGCGCTGCGTCCCGGCGA
TCGGGTGCGTATCCTGGCGAAAGGTTTACCCGCGCTGGAAAATCCGGTTGTCGCAGAAGATGAATTCGCCCGCCACCAGA
CGTTTACGTGGCCGTTGTCAGCAACGGGAACGCTATTTGCGCTGGGGTTGAACTACGCCGATCACGCCAGCGAGCTGGCA
TTTACGCCGCCGAAAGAGCCGCTGGTATTTATCAAAGCGCCAAACACCTTTACCGAACATCATCAAACGTCGGTGCGCCC
GAACAACGTCGAATATATGCACTATGAAGCCGAACTGGTCGTGGTAATTGGCAAAACGGCGCGTAAGGTGAGCGAAGCCG
AAGCCATGGAGTATGTGGCCGGTTACACCGTCTGTAACGACTACGCGATCCGCGACTATCTGGAAAACTACTACCGTCCA
AATCTGCGGGTAAAAAGCCGCGACGGCCTGACGCCGATAGGCCCGTGGATTGTGGATAAAGAGGCGGTTTCTGATCCGCA
TAACCTGACGTTACGCACCTTTGTCAACGGTGAGCTGCGGCAGGAAGGGACGACCGCCGATCTGATCTTCAGCATCCCGT
TCCTGATTTCTTATCTGAGCGAATTTATGACGTTGCAACCGGGCGACATGATTGCCACCGGTACGCCGAAAGGGTTGTCC
GATGTGGTGCCGGGGGATGAAGTTGTCGTGGAAGTAGAAGGCGTGGGTCGCCTGGTTAACCGAATCGTCAGTGAGGAGAG
CGCAAAATGA

Upstream 100 bases:

>100_bases
ACTTTACTTCAGCTTGCTAAATGTACAGCGAAAGCATAAATCTAATCATTAATATATTAATGAAATCACAGCCCGTTAAA
TCGGCCTGAGGAGTTTATGT

Downstream 100 bases:

>100_bases
AGAAAATAAATCATTGGATTAACGGCAAAAAAGTTGCAGGTAACGACTACTTCCAAACCACTAACCCGGCGACTGGTGAC
GTGCTGGCGGAAGTGGCCTC

Product: 4-hydroxyphenylacetate degradation bifunctionalisomerase/decarboxylase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKGTVFAVALNHRSQLDAWQEAFSQPPYNAPPKTAVWFIKPRNTVIRHGEPIPYPQGEKVLSGATVALIVGKTASRIRPE
AAADYIAGYALANEVSLPEESFYRPAIKAKCRDGFCPLGEMAPLSDVDNLTIITEINGREADHWNTADLQRSAAQLLSAL
SEFATLNPGDAILLGTPQNRVALRPGDRVRILAKGLPALENPVVAEDEFARHQTFTWPLSATGTLFALGLNYADHASELA
FTPPKEPLVFIKAPNTFTEHHQTSVRPNNVEYMHYEAELVVVIGKTARKVSEAEAMEYVAGYTVCNDYAIRDYLENYYRP
NLRVKSRDGLTPIGPWIVDKEAVSDPHNLTLRTFVNGELRQEGTTADLIFSIPFLISYLSEFMTLQPGDMIATGTPKGLS
DVVPGDEVVVEVEGVGRLVNRIVSEESAK

Sequences:

>Translated_429_residues
MKGTVFAVALNHRSQLDAWQEAFSQPPYNAPPKTAVWFIKPRNTVIRHGEPIPYPQGEKVLSGATVALIVGKTASRIRPE
AAADYIAGYALANEVSLPEESFYRPAIKAKCRDGFCPLGEMAPLSDVDNLTIITEINGREADHWNTADLQRSAAQLLSAL
SEFATLNPGDAILLGTPQNRVALRPGDRVRILAKGLPALENPVVAEDEFARHQTFTWPLSATGTLFALGLNYADHASELA
FTPPKEPLVFIKAPNTFTEHHQTSVRPNNVEYMHYEAELVVVIGKTARKVSEAEAMEYVAGYTVCNDYAIRDYLENYYRP
NLRVKSRDGLTPIGPWIVDKEAVSDPHNLTLRTFVNGELRQEGTTADLIFSIPFLISYLSEFMTLQPGDMIATGTPKGLS
DVVPGDEVVVEVEGVGRLVNRIVSEESAK
>Mature_429_residues
MKGTVFAVALNHRSQLDAWQEAFSQPPYNAPPKTAVWFIKPRNTVIRHGEPIPYPQGEKVLSGATVALIVGKTASRIRPE
AAADYIAGYALANEVSLPEESFYRPAIKAKCRDGFCPLGEMAPLSDVDNLTIITEINGREADHWNTADLQRSAAQLLSAL
SEFATLNPGDAILLGTPQNRVALRPGDRVRILAKGLPALENPVVAEDEFARHQTFTWPLSATGTLFALGLNYADHASELA
FTPPKEPLVFIKAPNTFTEHHQTSVRPNNVEYMHYEAELVVVIGKTARKVSEAEAMEYVAGYTVCNDYAIRDYLENYYRP
NLRVKSRDGLTPIGPWIVDKEAVSDPHNLTLRTFVNGELRQEGTTADLIFSIPFLISYLSEFMTLQPGDMIATGTPKGLS
DVVPGDEVVVEVEGVGRLVNRIVSEESAK

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=249, Percent_Identity=34.9397590361446, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI40786394, Length=249, Percent_Identity=34.9397590361446, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI66348062, Length=194, Percent_Identity=41.2371134020619, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI215422413, Length=197, Percent_Identity=40.6091370558376, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI13654274, Length=201, Percent_Identity=39.8009950248756, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1787428, Length=193, Percent_Identity=38.3419689119171, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=37.4331550802139, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324161, Length=228, Percent_Identity=26.3157894736842, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28572127, Length=219, Percent_Identity=41.5525114155251, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28571789, Length=210, Percent_Identity=39.5238095238095, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24663695, Length=217, Percent_Identity=34.1013824884793, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 47173; Mature: 47173

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTVFAVALNHRSQLDAWQEAFSQPPYNAPPKTAVWFIKPRNTVIRHGEPIPYPQGEKV
CCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCHH
LSGATVALIVGKTASRIRPEAAADYIAGYALANEVSLPEESFYRPAIKAKCRDGFCPLGE
HCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHCCCCCCCCCC
MAPLSDVDNLTIITEINGREADHWNTADLQRSAAQLLSALSEFATLNPGDAILLGTPQNR
CCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
VALRPGDRVRILAKGLPALENPVVAEDEFARHQTFTWPLSATGTLFALGLNYADHASELA
EEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCEEEEECCCCCEEEEEECCCHHHCCCCC
FTPPKEPLVFIKAPNTFTEHHQTSVRPNNVEYMHYEAELVVVIGKTARKVSEAEAMEYVA
CCCCCCCEEEEECCCCHHHCCCCCCCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHH
GYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPIGPWIVDKEAVSDPHNLTLRTFVNGELR
HHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECHHHCCCCCCEEEEEEECCHHH
QEGTTADLIFSIPFLISYLSEFMTLQPGDMIATGTPKGLSDVVPGDEVVVEVEGVGRLVN
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHCCCCCEEEEEECHHHHHHH
RIVSEESAK
HHHHHCCCC
>Mature Secondary Structure
MKGTVFAVALNHRSQLDAWQEAFSQPPYNAPPKTAVWFIKPRNTVIRHGEPIPYPQGEKV
CCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCHH
LSGATVALIVGKTASRIRPEAAADYIAGYALANEVSLPEESFYRPAIKAKCRDGFCPLGE
HCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHCCCCCCCCCC
MAPLSDVDNLTIITEINGREADHWNTADLQRSAAQLLSALSEFATLNPGDAILLGTPQNR
CCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
VALRPGDRVRILAKGLPALENPVVAEDEFARHQTFTWPLSATGTLFALGLNYADHASELA
EEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCEEEEECCCCCEEEEEECCCHHHCCCCC
FTPPKEPLVFIKAPNTFTEHHQTSVRPNNVEYMHYEAELVVVIGKTARKVSEAEAMEYVA
CCCCCCCEEEEECCCCHHHCCCCCCCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHH
GYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPIGPWIVDKEAVSDPHNLTLRTFVNGELR
HHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECHHHCCCCCCEEEEEEECCHHH
QEGTTADLIFSIPFLISYLSEFMTLQPGDMIATGTPKGLSDVVPGDEVVVEVEGVGRLVN
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHCCCCCEEEEEECHHHHHHH
RIVSEESAK
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA